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Gene information
General information
TC004142 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 1343235..1350950
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004142
    iBeetle screen iB_03699
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004142 RA
      MPLFGKEKK TKKDGKDSD KQPSLEDKY YLKELLGTG 
      AFSVVRLAE SKEKPGQVY AVKIIDKKA LKGKEDSLE 
      NEIKVLRRL THPNIVQLL ETFEDKSKV YLIMELVTG 
      GELFDRIVE KGSYTEKDA ADLIRQVLE AVDYMHEQG 
      VVHRDLKPE NLLYYSPDE DSKIMISDF GLSKMEDSG 
      IMATACGTP GYVAPEVLA QKPYGKAVD VWSIGVISY 
      ILLCGYPPF YDENDANLF AQILKGEFE FDSPYWDEI 
      SDSAKDFIR KLMCVNVEK RYTCRQALA HPWISGNAA 
      SNKNIHGTV AEQLKKNFA KSRWKQAYH ATTVIRQMQ 
      RMALSSGGS GGRSPVPQR SMSTGEPCG SLNGAQDK
    CDS sequence
    • >TC004142 RA
      atgcctctg tttggtaag gagaagaaa acgaaaaaa 
      gatgggaaa gactcagat aagcaacct tcactcgaa 
      gacaaatac tacttaaaa gaattactc ggcacgggc 
      gcattttcc gttgtcagg ctggccgaa agcaaggaa 
      aagccaggc caggtctac gccgtcaaa ataatcgac 
      aaaaaagct ttaaagggt aaagaagat tcgctcgaa 
      aatgaaatt aaggtgcta cgaagatta actcatcct 
      aatattgta cagttatta gaaacgttt gaagacaaa 
      tcgaaggtt tatttgata atggaatta gtgacgggt 
      ggcgaatta tttgatcgc attgttgag aagggttca 
      tacacggaa aaagacgca gctgatctc atacgacaa 
      gtcctagag gctgtagac tatatgcat gagcaaggt 
      gtagtccat cgtgatctg aagcctgaa aacctgctt 
      tattatagt ccagacgaa gatagcaaa ataatgatt 
      agtgatttc ggtttatca aaaatggaa gattctggg 
      atcatggcc actgcttgt gggactcca ggttacgtc 
      gctccggag gtcctcgcg cagaaaccg tacgggaag 
      gcggtggac gtctggagc ataggcgtg atatcttac 
      atcctcctc tgcggttat ccccctttt tatgacgaa 
      aacgacgca aatttattt gcacaaatc ctcaaaggc 
      gaatttgag ttcgattcg ccgtactgg gatgaaatc 
      agtgattct gcgaaagac tttattagg aaattgatg 
      tgtgttaat gtcgagaag cgctatact tgtcgccag 
      gctttggct catccttgg atatccggg aatgcggcc 
      agtaataag aacattcac ggtaccgtg gctgagcaa 
      cttaaaaag aatttcgca aaatcgcgc tggaagcaa 
      gcatatcat gctacgacg gtgattcga caaatgcaa 
      cgaatggcg ctaagtagt ggtggtagt gggggtcgt 
      agtccggta cctcaacgt agtatgtcc actggtgaa 
      ccatgtggg agcctaaac ggtgcccaa gataagtaa
    mRNA sequence
    • >TC004142 RA
      ttggtgttg ttcaggcag tgattttga gaaaaataa 
      ggagaatat cggttggtg gagtccagt gctagtccg 
      aaatgcact tatttcggt aaatgtgtg atatgaagg 
      cgtttggcg agctcgggg tgaagacct ttgatacta 
      gagttcggt ttgttgttg gtagcagta gtcggtaag 
      acgttgtaa ttttgacgg ttgtgtggc agctaggct 
      atcagtgta cagttttat cgcagttta actgtgata 
      agcaatata gatagcaat tataagaac acaataaac 
      gtttggagc aggtgaata acgcgcacg agtgtatag 
      taatgttgt gcgttgtct catgcgtgt agaagtgca 
      acatttatg tgaggagct cgagtaaaa aggaaattg 
      gaaaaatgc ctctgtttg gtaaggaga agaaaacga 
      aaaaagatg ggaaagact cagataagc aaccttcac 
      tcgaagaca aatactact taaaagaat tactcggca 
      cgggcgcat tttccgttg tcaggctgg ccgaaagca 
      aggaaaagc caggccagg tctacgccg tcaaaataa 
      tcgacaaaa aagctttaa agggtaaag aagattcgc 
      tcgaaaatg aaattaagg tgctacgaa gattaactc 
      atcctaata ttgtacagt tattagaaa cgtttgaag 
      acaaatcga aggtttatt tgataatgg aattagtga 
      cgggtggcg aattatttg atcgcattg ttgagaagg 
      gttcataca cggaaaaag acgcagctg atctcatac 
      gacaagtcc tagaggctg tagactata tgcatgagc 
      aaggtgtag tccatcgtg atctgaagc ctgaaaacc 
      tgctttatt atagtccag acgaagata gcaaaataa 
      tgattagtg atttcggtt tatcaaaaa tggaagatt 
      ctgggatca tggccactg cttgtggga ctccaggtt 
      acgtcgctc cggaggtcc tcgcgcaga aaccgtacg 
      ggaaggcgg tggacgtct ggagcatag gcgtgatat 
      cttacatcc tcctctgcg gttatcccc ctttttatg 
      acgaaaacg acgcaaatt tatttgcac aaatcctca 
      aaggcgaat ttgagttcg attcgccgt actgggatg 
      aaatcagtg attctgcga aagacttta ttaggaaat 
      tgatgtgtg ttaatgtcg agaagcgct atacttgtc 
      gccaggctt tggctcatc cttggatat ccgggaatg 
      cggccagta ataagaaca ttcacggta ccgtggctg 
      agcaactta aaaagaatt tcgcaaaat cgcgctgga 
      agcaagcat atcatgcta cgacggtga ttcgacaaa 
      tgcaacgaa tggcgctaa gtagtggtg gtagtgggg 
      gtcgtagtc cggtacctc aacgtagta tgtccactg 
      gtgaaccat gtgggagcc taaacggtg cccaagata 
      agtaatcaa attttttaa ctaccgaac taccgttca 
      tttctgcta cgcattttt aattggggt tgaaacatg 
      ttcttaaaa ttcgttact tttatggaa gttgaatta 
      gttagacat ttaaattaa cttatttta aggtcatga 
      tttataagc tattatggt cagaaggcc agctcactt 
      aagagaaga cttccaaaa caaaataat gatgtgatg 
      tatgtatca tgcaaacgt aacagtgtg taaaaatta 
      tccaacaat ctaatggca tatctgtta ttttgcttt 
      gcagatcta aagtttgtg tatattttc tttattttt 
      ttatttgtt ttacgtatt tttatatga atttttgtt 
      acgaaagct ctctttatt tctcaaata tacttaact 
      tttgtttag ctttagttt ttttactga ttgtttaat 
      gcacaaaat ttagtttat ttatggttt tgatagtta 
      acggtatta tgattatat tctaaaaag ttccgtaat 
      tacttagtg tataacata agattataa atcagacaa 
      aataaatgt tgaatatca ataataata atatatgaa 
      taataataa taataataa tagaaagca aatgttatt 
      gctatgtga cacataaat ctcgcatat tacgttttt 
      gtttgtaac tatagcgga attgtaggt tttcatcat 
      ggcattacc gaactgttc agaactggg aaaataatt 
      tattttgtt atgttgtta ttgttccaa gcaatatta 
      aatgttaga aatgttagt agtattaaa actaagaac 
      agaaataca ctaccttct aaaacacat atgcttgct 
      taagacata aatgtatta ttctgtgtg aggttcatt 
      tgatttttt tgttttatt ttgtatatg ttgtaacta 
      attgaacaa gtatatcat gttcaacat catttatca 
      agaaattgg tcgtcagaa acttaattt aaccactta 
      cactgaaac tattttttg aaacttatt atacaaaca 
      gtggcctac atgaaagcc tattgatcg gccatttga 
      aatgatatt atatccgtc ctttggatt atgttgaat 
      tgtgatttc gtgaaattg gccttgaat tttgaacat 
      gatatacgt aaacgtagt tcatttttt ctctaaact 
      cacaacatg tgatatgta ctatatttc atatctatt 
      ttatttatt tccttataa ggtgtacat tgtataaac 
      gaactaagt agagtttca gtgtatatt tttaggaat 
      ttgttgttt ttgcactgc atatatacg cctgtgtag 
      cataaaata gatatttag ttttatatt tttactaaa 
      tgtacaaac tgctacaaa tggtttatc aggtgtata 
      tttcttaaa acattctat gtaattttt tagttcaat 
      aatactgtg atgttatac agtaagaca tttagtata 
      cagttatat cttcctaaa tcatagggt gagctttta 
      ttatccgca taaaacaat tggattttg tcaaccact 
      aagacacta gtacgttat tttattgaa agagtataa 
      tttgtgacg ggaaataaa aactcacag gaagtgagc 
      aattatagg ataatttaa tggagcatt tcttcacat 
      tccaattat cgttactta atatgcatt tatttaaaa 
      gaaataact acgttcagt ttaggtaaa aaataaaca 
      tacatcgat caaactcta aacgctttt gtgttaatt 
      ttgacagac aaaagtttc gcaacgtta tcgctgatg 
      ttttaaatg aaatgtata tactaaatt aagcaattg 
      taaatgacc actgaaaag gcaatgtag ttttatgca 
      gaaaatagc ttttttgta attttataa ttggtttat 
      gaagattag aacaaagct atagcgtcg ctttaatct 
      tgttttagc ctctaaata taaaaatat tcattggaa 
      tgcagtgtt caaatattt taaatttta attattgtt 
      aataatact ttgtttcta cc