Status
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Gene information
General information
TC006136 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 3115024..3118918
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC006136
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_03698

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC006136 RA
        MAPTKENEL KSLQGKRQS LFLRIQRLY NDSRNLDDE 
        TVCKNFKIR YNTLEETRR LFSNCIDSI NLLSLELDS 
        DYTPSFAEL EAVDELYCH IVEAAKKVF TKTENLLKP 
        VKAIAKLPK IELMEFSGE MSDWPIFYD TFRTLIHEN 
        PDLDDITRM HYLLGKLTG KALLVCSGI QPTGANYPI 
        LWKALVEKY DDKRLLANA YLSQILDFR PLQNESVVC 
        LNTFLEKFD TAVNALKQL DIENLSDFI ISSIALSKL 
        DGQTRRHFE LSQKKSEIP LYKEIVDFV KDHTKILAC 
        TLKSTATTA NRTNYSAPF SKPSRTHSL VVSNRASNN 
        ICPICNEAS HLVYQCAKL LKLGPLERY QLVKDKALC 
        LNCLSPKHK IIACKSTTT CSLCRKRHH SLLHFDKPH 
        IETSEQPAK KDLVPISDV NSTSREPHS FCVASNTSF 
        KSSRYSNTL LATAIVEIF APNSKKKLV RLLVDPASQ 
        SHFITTNCC TRLNLPIRK INASVKGIG AITHPIKGK 
        IDTVIASRF NSDYRYKFE ALVIDQIID NLPDRNIPQ 
        SSIENLLNL PLADEKFFE SGEIDGIIS ARLIHFVES 
        TFQSRLIIE NIVALTDSS VVLNWINAS PHRWHTFVA 
        NRVAKIQEL VPSVNWYHV DGKENPADP ISRGLTPAH 
        LLNHPIWLT GPSWLFMDE QLWPINSST NTDEPPLEE 
        KPIVLVTFS RCVNPLRQL IDRSSSYSK LRNAVVYVL 
        RFLKILPKG NLITLKDLE TAELTLVRL VQREHFASD 
        LALLEQNKP CSPKIRALC PFLKDGVIR VGGRLSNSN 
        LEYDQQHPF LLPKKDHLV DLLIDYFHQ RNLHTGPHL 
        TLSLLRQKF WILAARNVV RHRIRNCNY CFKFRPRAT 
        FPCMADLPA PRITEAKPF LHSGVDYAG PFYITLTRR 
        RGVKSQKAY LCLFICLVT KALHLEVAS DLSTATFIN 
        AFKRFLSRR GPCSVLYSD CGTNFIGAK TALDDLSKF 
        VTSQEYHSS IDDELIKRN VVWKFNPPA APHFGGIWE 
        ANIKSVKSH LTKVIGTQI LTYEEFTTV ITQIEALLN 
        SRPLCTLSS DPNEPLALT PAHFLTGTP LQSLPIQNL 
        DMSTNLVTR KELLDQILQ TYWKRWHME YLHNLQVRQ 
        KWNKPSSPI KVGTVVVLR TDNTPPLHW PLGVVQEVF 
        PGKDGIVRV ASVKTPNGL YKRPIVRLC PFQISDRFD 
        PIILFLVIC SLGGCNYLQ FNLI
      CDS sequence
      • >TC006136 RA
        atggctcca actaaagag aatgaatta aagtctctt 
        caaggcaag cgtcaatca ctttttttg agaattcag 
        cgtttatat aacgactct agaaacctg gatgatgag 
        acggtttgc aaaaacttt aaaattaga tataataca 
        ctagaagaa acgcgccga ttgtttagt aattgcatt 
        gattcgata aatttatta agtttagaa ctcgactct 
        gactatacg cctagcttt gctgagttg gaggcggtg 
        gacgaatta tattgtcac attgtagaa gcagcaaaa 
        aaggttttt acaaaaact gaaaatttg ctcaagccg 
        gtgaaagct atagcaaaa ttgcccaag atcgaattg 
        atggaattt tcaggggaa atgtccgat tggccaatt 
        ttttatgac acgtttaga actttaata cacgaaaat 
        cccgattta gacgatatt actagaatg cattactta 
        ttgggaaaa ttgacgggt aaagctttg ctggtttgc 
        tctggaata caaccaaca ggagcaaat tatcccatc 
        ttatggaaa gcgctcgta gaaaaatat gatgataaa 
        cgtcttctg gctaatgct tatttgtcg caaattctt 
        gactttaga cctttacaa aatgaatcg gtagtttgt 
        ttaaataca tttttggaa aagtttgat acagcggtg 
        aacgctttg aaacaatta gacatagaa aatctttcg 
        gactttatt ataagttca atcgcactt tcaaaatta 
        gatggtcaa actcgtaga cattttgag ctgtcacag 
        aaaaaatct gaaattcct ttgtacaag gaaattgtt 
        gactttgta aaggatcac acaaaaatc ttagcttgt 
        actttgaaa tcaactgct acaacagcc aatcgaacg 
        aactatagc gctcctttc agtaaacct agtagaacc 
        cattcgctt gttgttagt aatcgcgca tcgaataat 
        atttgtcca atttgtaac gaggcttct catttagtt 
        tatcagtgt gcgaagctt ttaaagtta ggtcccttg 
        gaaagatat caattagtc aaagataaa gctttatgt 
        ttaaattgc cttagtccg aaacataaa ataatcgct 
        tgtaaatcg acaaccact tgtagtttg tgtcgaaaa 
        agacatcat tctctgctt cattttgat aaaccgcac 
        attgagaca tcagaacag cctgctaaa aaggatttg 
        gttccaatt agtgacgtt aatagtact tcccgcgag 
        ccccatagt ttttgtgtc gcttcaaat actagcttt 
        aaatcatct agatatagt aacactttg cttgctacc 
        gcaatagta gaaatattc gctccaaat tcgaaaaag 
        aaattagtt agactttta gtagacccc gctagtcaa 
        tcgcatttt attactaca aattgttgt acccgctta 
        aatttgcca attagaaaa attaacgct tctgttaaa 
        ggcataggt gccatcacg catcctata aaaggaaaa 
        atagatacg gtcattgct tctcgcttt aattcagat 
        tatagatac aagttcgag gctttagtt atcgatcaa 
        attattgac aatttgcca gatcgaaat attccgcaa 
        tcatctata gaaaatttg cttaatctt ccgctagcc 
        gacgagaaa ttttttgag tcgggggaa atcgatgga 
        ataattagt gctagactg atccatttt gtagaaagc 
        actttccaa tcgcgatta attatagaa aacattgtt 
        gctctgact gattcttcg gtcgtactt aactggata 
        aacgcatct cctcatcgt tggcacact tttgtagca 
        aatcgcgtg gcaaaaatt caagaatta gtgccgtcg 
        gttaattgg tatcatgtc gatgggaag gaaaaccca 
        gccgaccca atttctagg ggtctaaca ccggcgcac 
        cttttaaac cacccgatt tggctaact ggtcctagt 
        tggttattt atggacgaa cagttatgg cctattaac 
        tcttcaact aatacagac gaacctcca ctcgaagag 
        aaaccgata gttctcgtc actttctca cgttgtgtc 
        aacccttta cgacaactt atagataga tcgtcttcg 
        tatagtaaa ttgcgcaat gcagttgta tatgttctc 
        agatttctt aagatattg ccaaaagga aatcttatt 
        acactcaaa gacttagaa accgctgaa ttaacgcta 
        gttagactt gtccaaaga gaacatttt gcttcagat 
        ttagctttg ttagagcaa aataaacct tgttcgcct 
        aaaattcgt gccttatgt ccgtttctt aaagatgga 
        gttattaga gtaggagga cgtctatca aactcaaat 
        ttagaatat gatcagcag catccgttt ttgctgccc 
        aaaaaagac cacttggtg gatctctta atagactat 
        tttcaccaa cgtaattta catactggt ccgcatctt 
        acactgtcg ttgttgcgt cagaaattt tggattcta 
        gctgcacga aatgttgtt agacatcgc attcgcaac 
        tgtaattat tgctttaaa tttcgtcct cgcgcaact 
        ttcccatgt atggcggat ttgcccgct cctagaatt 
        acagaagcg aaaccattt ttgcacagt ggtgtagat 
        tatgccgga cctttctat atcactctg actcgtcgt 
        agaggtgta aaaagccag aaagcttat ttatgctta 
        tttatttgt ttagttacc aaagcctta catctagaa 
        gtggcttct gacctatcc actgcgact tttattaat 
        gcctttaaa cgtttttta tcccgtcgt ggtccatgc 
        tcggttttg tattccgat tgtggtaca aattttatt 
        ggagctaaa actgcccta gacgatttg tcaaaattt 
        gtgacctca caagagtat cattctagt attgatgat 
        gaattgata aaacgaaat gtagtatgg aaatttaat 
        cctccagca gcacctcac tttggagga atctgggag 
        gcaaacatt aaaagtgtg aagtctcat ttaactaaa 
        gttattggc acccaaatt ttgacttat gaagaattt 
        acgacggtt attactcaa atagaggct ttattaaat 
        tctcgacca ttgtgtacg ctcagctcg gatcctaac 
        gagccgtta gctttgacg cctgctcat tttttgaca 
        gggacgccc cttcaatcg ctaccaatt caaaattta 
        gacatgtcg actaattta gtaacccga aaagaactg 
        ctcgatcaa attttgcaa acgtattgg aaacgatgg 
        cacatggaa tatctacat aatttgcaa gttcgtcaa 
        aaatggaat aagccatct tctccaata aaggtcggt 
        acggtcgta gttttgcgg acggataac accccaccg 
        ttgcattgg ccattaggg gtcgttcaa gaggtcttt 
        cctggcaag gatgggata gttcgcgtc gccagcgtc 
        aaaactccc aatggtctt tataaacga cctatagtt 
        cgtctatgt cccttccaa atcagtgat cgttttgat 
        cctataatt ttgttttta gttatttgt tctttgggt 
        ggatgcaat tacttgcag tttaattta atttaa
      mRNA sequence
      • >TC006136 RA
        atggctcca actaaagag aatgaatta aagtctctt 
        caaggcaag cgtcaatca ctttttttg agaattcag 
        cgtttatat aacgactct agaaacctg gatgatgag 
        acggtttgc aaaaacttt aaaattaga tataataca 
        ctagaagaa acgcgccga ttgtttagt aattgcatt 
        gattcgata aatttatta agtttagaa ctcgactct 
        gactatacg cctagcttt gctgagttg gaggcggtg 
        gacgaatta tattgtcac attgtagaa gcagcaaaa 
        aaggttttt acaaaaact gaaaatttg ctcaagccg 
        gtgaaagct atagcaaaa ttgcccaag atcgaattg 
        atggaattt tcaggggaa atgtccgat tggccaatt 
        ttttatgac acgtttaga actttaata cacgaaaat 
        cccgattta gacgatatt actagaatg cattactta 
        ttgggaaaa ttgacgggt aaagctttg ctggtttgc 
        tctggaata caaccaaca ggagcaaat tatcccatc 
        ttatggaaa gcgctcgta gaaaaatat gatgataaa 
        cgtcttctg gctaatgct tatttgtcg caaattctt 
        gactttaga cctttacaa aatgaatcg gtagtttgt 
        ttaaataca tttttggaa aagtttgat acagcggtg 
        aacgctttg aaacaatta gacatagaa aatctttcg 
        gactttatt ataagttca atcgcactt tcaaaatta 
        gatggtcaa actcgtaga cattttgag ctgtcacag 
        aaaaaatct gaaattcct ttgtacaag gaaattgtt 
        gactttgta aaggatcac acaaaaatc ttagcttgt 
        actttgaaa tcaactgct acaacagcc aatcgaacg 
        aactatagc gctcctttc agtaaacct agtagaacc 
        cattcgctt gttgttagt aatcgcgca tcgaataat 
        atttgtcca atttgtaac gaggcttct catttagtt 
        tatcagtgt gcgaagctt ttaaagtta ggtcccttg 
        gaaagatat caattagtc aaagataaa gctttatgt 
        ttaaattgc cttagtccg aaacataaa ataatcgct 
        tgtaaatcg acaaccact tgtagtttg tgtcgaaaa 
        agacatcat tctctgctt cattttgat aaaccgcac 
        attgagaca tcagaacag cctgctaaa aaggatttg 
        gttccaatt agtgacgtt aatagtact tcccgcgag 
        ccccatagt ttttgtgtc gcttcaaat actagcttt 
        aaatcatct agatatagt aacactttg cttgctacc 
        gcaatagta gaaatattc gctccaaat tcgaaaaag 
        aaattagtt agactttta gtagacccc gctagtcaa 
        tcgcatttt attactaca aattgttgt acccgctta 
        aatttgcca attagaaaa attaacgct tctgttaaa 
        ggcataggt gccatcacg catcctata aaaggaaaa 
        atagatacg gtcattgct tctcgcttt aattcagat 
        tatagatac aagttcgag gctttagtt atcgatcaa 
        attattgac aatttgcca gatcgaaat attccgcaa 
        tcatctata gaaaatttg cttaatctt ccgctagcc 
        gacgagaaa ttttttgag tcgggggaa atcgatgga 
        ataattagt gctagactg atccatttt gtagaaagc 
        actttccaa tcgcgatta attatagaa aacattgtt 
        gctctgact gattcttcg gtcgtactt aactggata 
        aacgcatct cctcatcgt tggcacact tttgtagca 
        aatcgcgtg gcaaaaatt caagaatta gtgccgtcg 
        gttaattgg tatcatgtc gatgggaag gaaaaccca 
        gccgaccca atttctagg ggtctaaca ccggcgcac 
        cttttaaac cacccgatt tggctaact ggtcctagt 
        tggttattt atggacgaa cagttatgg cctattaac 
        tcttcaact aatacagac gaacctcca ctcgaagag 
        aaaccgata gttctcgtc actttctca cgttgtgtc 
        aacccttta cgacaactt atagataga tcgtcttcg 
        tatagtaaa ttgcgcaat gcagttgta tatgttctc 
        agatttctt aagatattg ccaaaagga aatcttatt 
        acactcaaa gacttagaa accgctgaa ttaacgcta 
        gttagactt gtccaaaga gaacatttt gcttcagat 
        ttagctttg ttagagcaa aataaacct tgttcgcct 
        aaaattcgt gccttatgt ccgtttctt aaagatgga 
        gttattaga gtaggagga cgtctatca aactcaaat 
        ttagaatat gatcagcag catccgttt ttgctgccc 
        aaaaaagac cacttggtg gatctctta atagactat 
        tttcaccaa cgtaattta catactggt ccgcatctt 
        acactgtcg ttgttgcgt cagaaattt tggattcta 
        gctgcacga aatgttgtt agacatcgc attcgcaac 
        tgtaattat tgctttaaa tttcgtcct cgcgcaact 
        ttcccatgt atggcggat ttgcccgct cctagaatt 
        acagaagcg aaaccattt ttgcacagt ggtgtagat 
        tatgccgga cctttctat atcactctg actcgtcgt 
        agaggtgta aaaagccag aaagcttat ttatgctta 
        tttatttgt ttagttacc aaagcctta catctagaa 
        gtggcttct gacctatcc actgcgact tttattaat 
        gcctttaaa cgtttttta tcccgtcgt ggtccatgc 
        tcggttttg tattccgat tgtggtaca aattttatt 
        ggagctaaa actgcccta gacgatttg tcaaaattt 
        gtgacctca caagagtat cattctagt attgatgat 
        gaattgata aaacgaaat gtagtatgg aaatttaat 
        cctccagca gcacctcac tttggagga atctgggag 
        gcaaacatt aaaagtgtg aagtctcat ttaactaaa 
        gttattggc acccaaatt ttgacttat gaagaattt 
        acgacggtt attactcaa atagaggct ttattaaat 
        tctcgacca ttgtgtacg ctcagctcg gatcctaac 
        gagccgtta gctttgacg cctgctcat tttttgaca 
        gggacgccc cttcaatcg ctaccaatt caaaattta 
        gacatgtcg actaattta gtaacccga aaagaactg 
        ctcgatcaa attttgcaa acgtattgg aaacgatgg 
        cacatggaa tatctacat aatttgcaa gttcgtcaa 
        aaatggaat aagccatct tctccaata aaggtcggt 
        acggtcgta gttttgcgg acggataac accccaccg 
        ttgcattgg ccattaggg gtcgttcaa gaggtcttt 
        cctggcaag gatgggata gttcgcgtc gccagcgtc 
        aaaactccc aatggtctt tataaacga cctatagtt 
        cgtctatgt cccttccaa atcagtgat cgttttgat 
        cctataatt ttgttttta gttatttgt tctttgggt 
        ggatgcaat tacttgcag tttaattta atttaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY