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Gene information
General information
TC004134 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 1183114..1188102
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004134
    iBeetle screen iB_03697
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs segment shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs segment distal potentiallyfused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004134 RA
      MFGNRPIHP IRVCVRARP LTQSEISAN APKVVKYMN 
      TKHLLVRRG VNKRKRTYD RVFSEHADQ IEVFRHTVA 
      RFISSITRG YSFTFFAYG PTGTGKSYT LFGEGEQAL 
      VTDFKKDRA IGLIQRAAY QLFCELEQL DSSRVEYTV 
      RLSFIEINH EQVFDLLVE NGPLRVYDD PLENGSVCI 
      KDMSKVTVF NADDVNDWV NVGRRTLRK SAYYAYLSH 
      LMVTFFVDI RETTPEGAH VLRTARLSF VEVAGGEHA 
      TEIDEGRTL DTAVVHQSL DAFEECIKA LGMQQRSIP 
      YSSAKLTRI VQNSLGGTA RACFMSTFS TSTQAWTEI 
      LSSLQIATL MKRVANYPR LNTARTLAD ATRELDGII 
      ARKETELAE MRQALGPYV RADANLTED QISANKKIV 
      DLLRELLEL REHRALLEA EYRRLDASF IRTKRFREY 
      AHAQLEADQ DLTEQQRRC LVRRFIARN NEMIQNANN 
      LLDMCEERI AQAKIPPRQ VQVQADASN ADLAKQVVE 
      VTTDALSDV EQATSSYKR QQLTRVVKM AQLNTYLRR 
      RFLADLQQI SDMMGKIKD LRPLDINDL LNDQARSSA 
      DQIAYFIDN IYGEVSHYM KLPDQPKAE IGKMNEGIA 
      ADIEEVTRT ETEVRAELD RHQAERSQH YNLVFRFFD 
      DLLKKLDER EARIRIQFD EGNASVECL NNEMQKAKR 
      DKEILVKEV LLLQEAMQF VRDQLNGKD GSFFEEASK 
      MMSVPGKMA FSFENLNVG EVSEQFSNT VNDFYTEST 
      EQAENIEDF TDDIVDAFK SVVQTHNNH NARTARQIQ 
      TIGKGVREN ILEKAYANT THIAKIAEG ASANQEYYA 
      SKTRNIVDQ VMDQLESIH GKIQFPPGE GEEASAAEN 
      DPENIENVA PRV
    CDS sequence
    • >TC004134 RA
      atgttcggt aatcgtccc attcacccg ataagggtg 
      tgcgtgagg gctaggcct ctgacccag agcgagatc 
      tctgcaaat gccccgaaa gtggtgaag tacatgaac 
      acgaagcac cttctggtc cgaaggggg gtaaacaag 
      aggaagcgc acctatgac cgcgttttt agcgagcac 
      gccgaccaa atcgaagtt tttcggcac acggttgcc 
      cgcttcatt agtagcatt actcgaggt tactcgttc 
      acgtttttc gcgtacggc ccaacgggc accggcaag 
      tcgtacacg ctgttcggg gaaggcgaa caggccctt 
      gttacggac ttcaagaag gatagggcg atcggtttg 
      attcagcga gctgcttac cagctgttt tgcgagtta 
      gagcagctc gactcgagc agggttgag tacacggtg 
      cgcctgtcg ttcattgaa attaaccac gaacaggtt 
      ttcgatttg cttgtggaa aatgggcct ttgagagtt 
      tacgatgat cccctggag aacggttcg gtttgcatt 
      aaagatatg tcgaaggtc acggtgttt aacgctgac 
      gatgtcaac gactgggtt aatgttggc cgcagaacg 
      ctcagaaag agtgcttat tacgcgtat ctttcgcac 
      ctgatggtc actttcttc gtggacatc agagaaacg 
      accccggag ggagcgcat gttcttaga accgctcgt 
      ttgagcttt gttgaggtg gctggtggt gaacatgcc 
      actgaaatt gacgaagga cgaacactg gacactgcc 
      gttgtccac cagtcgctt gatgcgttt gaagagtgc 
      attaaagcg ttggggatg caacaacgg tcaatccca 
      tactctagc gcgaaattg acccgaatt gtgcaaaac 
      agtcttggc ggaaccgcc agagcttgt tttatgtcg 
      acgttctca accagcact caagcctgg actgagatt 
      ttgagtagt ctgcagata gcgaccttg atgaaacgt 
      gttgccaat tatcccagg ttgaatact gctagaacc 
      ctagcagat gcgacacgt gagctggac ggaataatt 
      gcacgcaag gagactgaa ttggcggag atgcgccaa 
      gcgctaggt ccgtacgta agagcggac gccaatctc 
      accgaagat cagatcagt gccaacaag aaaattgtt 
      gatttattg cgtgaattg ctcgaattg cgggaacac 
      cgggcgctg cttgaagcc gagtaccgc aggctggac 
      gcttcgttt atccgaacg aagagattc cgggaatac 
      gcccacgcc caattggaa gcggaccag gacctgacc 
      gaacaacag agaagatgt ctcgtgcgc cgtttcatc 
      gcgcgaaac aacgaaatg atccaaaat gctaacaac 
      ttgctcgac atgtgcgag gaaaggatt gcacaagca 
      aagattccg ccgcgccaa gttcaggtg caagccgat 
      gcaagtaac gccgatttg gctaagcaa gtcgttgaa 
      gtcacgact gatgctttg agcgatgtt gaacaagcg 
      acgagctcg tacaaacgc cagcaactt acacgagtt 
      gtgaagatg gcacaactt aacacctac ttgcgaaga 
      cgcttcttg gccgatttg caacaaatt agcgatatg 
      atgggcaaa attaaagac ttgcgtccg ttggatatc 
      aacgatttg ctcaatgac caagcacgc tcctctgcc 
      gatcaaatc gcgtatttt atcgacaat atttacggc 
      gaagtttcg cactatatg aaactacca gaccagccc 
      aaagccgag attgggaaa atgaatgag gggattgct 
      gcagacatc gaagaagtt acacgaact gagacggag 
      gttcgcgct gagttggat cgccatcag gccgagaga 
      agccagcat tataacctc gtgttccgt ttctttgat 
      gacctttta aagaaattg gatgagcgc gaagctaga 
      attcgcatt cagttcgac gaagggaac gcttcggtt 
      gaatgtttg aataacgaa atgcaaaag gctaaacgc 
      gacaaagag atcctcgtg aaggaggtg ttacttctg 
      caggaagca atgcaattt gtccgtgac cagctcaat 
      gggaaagac ggcagcttc ttcgaggaa gcgagcaag 
      atgatgagc gttccggga aagatggca ttctcgttc 
      gaaaatttg aacgtgggt gaagtttcg gaacaattt 
      agcaacacc gtgaatgat ttttacacg gaatccacc 
      gaacaggcg gaaaacatc gaggatttc actgatgat 
      atcgtagat gcttttaag tcggttgtt cagactcac 
      aataatcac aatgcccgc acggctagg caaatccaa 
      actattggg aaaggtgtc agggaaaac attttggaa 
      aaggcgtat gctaacact acccatatt gcaaaaatt 
      gccgaaggg gcaagcgcc aatcaggag tattacgct 
      tccaaaacg aggaatatc gttgatcag gtgatggat 
      cagctggaa tcgattcat gggaagatt cagttcccg 
      ccaggcgaa ggagaggag gcttctgcc gcagagaac 
      gatcccgag aacattgaa aatgttgcc ccacgtgtt 
      tga
    mRNA sequence
    • >TC004134 RA
      cactggtga cttcagtcg ggccctgtt ggtgcgccc 
      tgatatcac taaaaaaaa ccgttaaaa accgttagt 
      gacgctgca cacgagctg tgataacta cctgtgatt 
      acattgtaa aaaacctgt gaaaatgtt cggtaatcg 
      tcccattca cccgataag ggtgtgcgt gagggctag 
      gcctctgac ccagagcga gatctctgc aaatgcccc 
      gaaagtggt gaagtacat gaacacgaa gcaccttct 
      ggtccgaag gggggtaaa caagaggaa gcgcaccta 
      tgaccgcgt ttttagcga gcacgccga ccaaatcga 
      agtttttcg gcacacggt tgcccgctt cattagtag 
      cattactcg aggttactc gttcacgtt tttcgcgta 
      cggcccaac gggcaccgg caagtcgta cacgctgtt 
      cggggaagg cgaacaggc ccttgttac ggacttcaa 
      gaaggatag ggcgatcgg tttgattca gcgagctgc 
      ttaccagct gttttgcga gttagagca gctcgactc 
      gagcagggt tgagtacac ggtgcgcct gtcgttcat 
      tgaaattaa ccacgaaca ggttttcga tttgcttgt 
      ggaaaatgg gcctttgag agtttacga tgatcccct 
      ggagaacgg ttcggtttg cattaaaga tatgtcgaa 
      ggtcacggt gtttaacgc tgacgatgt caacgactg 
      ggttaatgt tggccgcag aacgctcag aaagagtgc 
      ttattacgc gtatctttc gcacctgat ggtcacttt 
      cttcgtgga catcagaga aacgacccc ggagggagc 
      gcatgttct tagaaccgc tcgtttgag ctttgttga 
      ggtggctgg tggtgaaca tgccactga aattgacga 
      aggacgaac actggacac tgccgttgt ccaccagtc 
      gcttgatgc gtttgaaga gtgcattaa agcgttggg 
      gatgcaaca acggtcaat cccatactc tagcgcgaa 
      attgacccg aattgtgca aaacagtct tggcggaac 
      cgccagagc ttgttttat gtcgacgtt ctcaaccag 
      cactcaagc ctggactga gattttgag tagtctgca 
      gatagcgac cttgatgaa acgtgttgc caattatcc 
      caggttgaa tactgctag aaccctagc agatgcgac 
      acgtgagct ggacggaat aattgcacg caaggagac 
      tgaattggc ggagatgcg ccaagcgct aggtccgta 
      cgtaagagc ggacgccaa tctcaccga agatcagat 
      cagtgccaa caagaaaat tgttgattt attgcgtga 
      attgctcga attgcggga acaccgggc gctgcttga 
      agccgagta ccgcaggct ggacgcttc gtttatccg 
      aacgaagag attccggga atacgccca cgcccaatt 
      ggaagcgga ccaggacct gaccgaaca acagagaag 
      atgtctcgt gcgccgttt catcgcgcg aaacaacga 
      aatgatcca aaatgctaa caacttgct cgacatgtg 
      cgaggaaag gattgcaca agcaaagat tccgccgcg 
      ccaagttca ggtgcaagc cgatgcaag taacgccga 
      tttggctaa gcaagtcgt tgaagtcac gactgatgc 
      tttgagcga tgttgaaca agcgacgag ctcgtacaa 
      acgccagca acttacacg agttgtgaa gatggcaca 
      acttaacac ctacttgcg aagacgctt cttggccga 
      tttgcaaca aattagcga tatgatggg caaaattaa 
      agacttgcg tccgttgga tatcaacga tttgctcaa 
      tgaccaagc acgctcctc tgccgatca aatcgcgta 
      ttttatcga caatattta cggcgaagt ttcgcacta 
      tatgaaact accagacca gcccaaagc cgagattgg 
      gaaaatgaa tgaggggat tgctgcaga catcgaaga 
      agttacacg aactgagac ggaggttcg cgctgagtt 
      ggatcgcca tcaggccga gagaagcca gcattataa 
      cctcgtgtt ccgtttctt tgatgacct tttaaagaa 
      attggatga gcgcgaagc tagaattcg cattcagtt 
      cgacgaagg gaacgcttc ggttgaatg tttgaataa 
      cgaaatgca aaaggctaa acgcgacaa agagatcct 
      cgtgaagga ggtgttact tctgcagga agcaatgca 
      atttgtccg tgaccagct caatgggaa agacggcag 
      cttcttcga ggaagcgag caagatgat gagcgttcc 
      gggaaagat ggcattctc gttcgaaaa tttgaacgt 
      gggtgaagt ttcggaaca atttagcaa caccgtgaa 
      tgattttta cacggaatc caccgaaca ggcggaaaa 
      catcgagga tttcactga tgatatcgt agatgcttt 
      taagtcggt tgttcagac tcacaataa tcacaatgc 
      ccgcacggc taggcaaat ccaaactat tgggaaagg 
      tgtcaggga aaacatttt ggaaaaggc gtatgctaa 
      cactaccca tattgcaaa aattgccga aggggcaag 
      cgccaatca ggagtatta cgcttccaa aacgaggaa 
      tatcgttga tcaggtgat ggatcagct ggaatcgat 
      tcatgggaa gattcagtt cccgccagg cgaaggaga 
      ggaggcttc tgccgcaga gaacgatcc cgagaacat 
      tgaaaatgt tgccccacg tgtttgaat ctcccccaa 
      gaagggaag gagaagtaa gtggtttaa gttacatta 
      acacagtta atatttcgt tttgattat taaacgttg 
      ggaaagtac ttgttttaa ttttatctg cagcatgcg 
      atttttttt tgtttttgt ggtggttac caaagttcc 
      tcttgttgc ttgcgaaaa ttgcttaca ttgctttaa 
      aattaccat aatcagatt atcgcccgg tatttcagt 
      tgactgttt aaaccaaag atattttcg ttatcaatt 
      tctttcctg gtttgacgt atttgagtg cggttttgc 
      caaaaagcc aaagatttt acccaagtt ctgaaatac 
      caggcgtta atgtgataa gcattaccc actttttta 
      aaaactgag tgcttggtt tagggtttt tttaatttt 
      atttgacgt tctgagttc ttaataaaa attactttc 
      agta