Status
Please wait ...
Gene information
General information
TC004127 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1533167..1577906
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004127
    iBeetle screen iB_06566

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     larva partially dorsalnot closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_03694

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004127 RA
      MAVLIKKCS LILPGFRTT SCLTVVLLV CMETVFLCT 
QSTCSKTFQ VHWNTSDSI FHIDNTDHI IDVNKGNAQ 
FEYDQVNII CPVYTPGTY GDDLEKYII YNVSKEEYE 
TCRITNPNP RIIAICDKP YKLMYFTIT FRPFTPQPG 
GLEFLPGKD YYFISTSSK DDLHRRIGG RCSSNNMKI 
VFKVCCAPD QSQANFTTK LTSTSTTTL GWPDIHRPM 
VEATSSSTT QWAPRTDAP PTRKPTKKM NEYEKHPNE 
VMKNEELTY SKGVWLSAG PPSFLVVIA CIAVIRWR
    CDS sequence
    • >TC004127 RA
      atggccgtg ttaataaag aagtgcagt ttgatcttg 
ccggggttt agaacaacc tcctgcctg accgttgtg 
ttgctcgtc tgcatggag accgtcttc ttgtgcacg 
cagtccacc tgctcaaaa acattccag gttcactgg 
aataccagc gatagcata tttcacatc gacaacact 
gaccacatc atcgacgta aataagggc aacgcccaa 
tttgaatac gaccaagtc aacatcatc tgtccggtg 
tacacgcca ggaacgtac ggagatgac ttagaaaaa 
tacatcatt tacaacgta tcgaaggag gagtacgaa 
acctgccga ataaccaac cccaacccc cgaataata 
gccatatgc gacaaaccg tacaaactt atgtatttc 
acaattaca tttcgccct tttacgccc cagcccggc 
ggcttggag ttcctcccc ggcaaagat tactacttt 
atatcgacg tcgtcaaag gacgacctc caccgccgg 
atcgggggc cggtgctcc tccaacaac atgaaaatc 
gtcttcaaa gtgtgctgc gcccccgac cagagccag 
gccaacttc acgacgaaa ttaacctcg acctcgacg 
acgacgctg ggctggccg gacatccac cggcccatg 
gtcgaagcg acgtcgtcg tcgaccacg caatgggcc 
cctcggacg gacgcgccg ccgacgcgg aaacccacc 
aagaaaatg aacgagtac gagaagcat ccgaacgaa 
gtcatgaaa aatgaagag ttgacgtac agcaaagga 
gtctggcta tcggcgggc ccgccgagt tttctagtt 
gtcattgcc tgcatcgca gtcatccga tggcggtga
    mRNA sequence
    • >TC004127 RA
      agagacgga ggacggagc cggccgccg ccaccggcc 
ctcacctgc tgatcctcg tcgattttc gtcgagaag 
cgacgaccg gaggccgag ggccgacca actgccgcg 
ttgccgatc agcgcacca cgccggcga ggccgagtc 
caagagaaa tagagagta aagagtaga gagtagaac 
gcgttgggc cgttggagg aagaaatca cgtccgcga 
gcacttcgg cacttccac gtcgcgatt cgaacatgt 
gccgggaca atgagagtt tctggtggc ggtcggccg 
gtgattgtt gagaatgac gacaacccc tccatggta 
gaagggtgg tcttggtgt tcagtgcaa gagtgcgtt 
ttggtggcc ggcgcgcga aatggccgt gttaataaa 
gaagtgcag tttgatctt gccggggtt tagaacaac 
ctcctgcct gaccgttgt gttgctcgt ctgcatgga 
gaccgtctt cttgtgcac gcagtccac ctgctcaaa 
aacattcca ggttcactg gaataccag cgatagcat 
atttcacat cgacaacac tgaccacat catcgacgt 
aaataaggg caacgccca atttgaata cgaccaagt 
caacatcat ctgtccggt gtacacgcc aggaacgta 
cggagatga cttagaaaa atacatcat ttacaacgt 
atcgaagga ggagtacga aacctgccg aataaccaa 
ccccaaccc ccgaataat agccatatg cgacaaacc 
gtacaaact tatgtattt cacaattac atttcgccc 
ttttacgcc ccagcccgg cggcttgga gttcctccc 
cggcaaaga ttactactt tatatcgac gtcgtcaaa 
ggacgacct ccaccgccg gatcggggg ccggtgctc 
ctccaacaa catgaaaat cgtcttcaa agtgtgctg 
cgcccccga ccagagcca ggccaactt cacgacgaa 
attaacctc gacctcgac gacgacgct gggctggcc 
ggacatcca ccggcccat ggtcgaagc gacgtcgtc 
gtcgaccac gcaatgggc ccctcggac ggacgcgcc 
gccgacgcg gaaacccac caagaaaat gaacgagta 
cgagaagca tccgaacga agtcatgaa aaatgaaga 
gttgacgta cagcaaagg agtctggct atcggcggg 
cccgccgag ttttctagt tgtcattgc ctgcatcgc 
agtcatccg atggcggtg agctaaaag acaggaacg 
ggagcgggg cctcgtctc cagctccac gttcacaca 
tgttgacac ttatctgta aaatactgt aaattattg 
gatgacgtg tccgcgagt gcgtgaata acggcgaca 
ctctaaata gtttgttta taagaaact gcgtaaaag 
ccaattttt gtagataaa aatagtgcg ttaatttaa 
gtgatggta cgatttttc agcgcgaag ctggtgtga 
taaaagtat taaaagact caaagagac tccttgtct 
aattactcc tcgcaatta cgtaaaata ctgtgcaat 
ttttatcga aaagtttcc tcgactacc gcgaggagt 
aactagatt atttcgtac gtgtacata aaagtgata 
ttattgtca ttgaaacgc ttcttgtag tccttaatt 
cccaaaaat gccgactga aacacttaa aagtttggg 
aatcgcaaa tacgacact gaaatttac cggcttacg 
gaaggtatt atttcattc ttccactct tcgagtact 
tacgacgta cattcccgc taattctag gctgtctct 
acgcaatta catagttcc aagtccaaa tttgtttta 
tccgagctt ttgggtgtg tccgtctta aaatgtatt 
ttattatac tatgaaata ttagtgtgt gtcgtttag 
caaagactt gtataaagt gtagagcgt acgtgctat 
atattgtat gttcattta atggatgaa atattttgt 
acttctctt aaaaaattg tgtaaacaa tgcagtgta 
aaagtaaat ggttgtctg atcagaatt gtcagagct 
ctacagtag ttcatatga aactttcgt tgtcgaaaa 
ttgttgggg ccaaccaga aattaccaa atcaagtgc 
gttttgtaa tccgggcgc ttgattcgc gtaatttta 
ttattttat aatagtttc ttcacagta ttatatttc 
atgtgcttt ttcttacgc ataagtttg tgtttccat 
attccttgg tgtctaatt tacctcaca ttaatacga 
tgcacctca ttattgtca tgccagtat ttattttac 
ttataacat acatccatc aaaatttct cttatttcc 
tctcttcgt gcgaaatca tcgatcatt cgacgtgta 
atttttata cacacattt aaccagatg tcatgttat 
gtagcacct caactcctt ataatccgg tgcttacga 
aaatattaa tgtatatcg ataaactta ccgactaaa 
ttgaaaaat gattcatcc agtataaaa tatctcttc 
atgatgcct tgttgtatt tacatcacc gataatctc 
aaaacgttg acagatatt ttgttaatt aacaactcg 
ccacgtaaa tctttcgtc caataaaca attcgacgt 
tttaaagtt atcggtgaa gatatatgg actagaagt 
aggactgta gaggcactg agaataaac aggtacaca 
atac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY