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Gene information
General information
TC004119 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 1488374..1513993
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004119
    iBeetle screen iB_03692
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004119 RA
      MDEETDEFN FHIARYRPE ELQKLASKT 
      KFTRKEIQL MYRGFKQEC PTGMVDEDS 
      FKQIFSQFF PLGDATNYA HYVFNTIKQ 
      KQTGKISFE DFLSILSKV SRGSVQEKL 
      QWVFGLYDL NGDGLITKT EMVDVVSSI 
      YEMLGRATH PTVNESSAK EHVEKIFHL 
      IDTNKDGVV TIEELVQWC SRDEHVLKS 
      LETLDTVL
    CDS sequence
    • >TC004119 RA
      atggatgag gagactgac gaatttaat ttccatata 
      gcaaggtat aggccggaa gaattacag aaattagcg 
      tcgaaaaca aaatttacg aggaaagaa atccaatta 
      atgtaccga ggattcaaa caggaatgc cccactgga 
      atggtagac gaggactcc ttcaagcaa atcttctcg 
      cagtttttt ccattaggt gatgcaacg aactacgct 
      cattacgtc ttcaacaca atcaagcaa aagcaaaca 
      ggaaaaatc agttttgag gactttctt agcatattg 
      tccaaggtg tcccgaggc agcgtccag gagaagctc 
      cagtgggtg ttcgggctg tacgacttg aacggggac 
      ggcctcatc accaagacg gagatggtg gacgtcgtt 
      tcgagcatc tatgagatg ttgggccga gcgacgcat 
      ccaactgtt aacgaaagc tcggccaaa gaacacgta 
      gaaaaaata ttccatctg atagacacg aataaggac 
      ggcgtcgtc accatcgaa gaactggtg cagtggtgc 
      tctagggat gaacatgtt cttaaatct ttagaaact 
      ttggacact gtattgtga
    mRNA sequence
    • >TC004119 RA
      ctccagtca tgtaatgat gcgcgataa cgttgcgcc 
      ggtggttcc ttcagggat ctgttagta cactggcgt 
      ccgtggatt gggagtatc gtgcgtcgc gacgccgtc 
      gatcatacg tgcccacca cgcggactt gatgcatca 
      tggatgagg agactgacg aatttaatt tccatatag 
      caaggtata ggccggaag aattacaga aattagcgt 
      cgaaaacaa aatttacga ggaaagaaa tccaattaa 
      tgtaccgag gattcaaac aggaatgcc ccactggaa 
      tggtagacg aggactcct tcaagcaaa tcttctcgc 
      agttttttc cattaggtg atgcaacga actacgctc 
      attacgtct tcaacacaa tcaagcaaa agcaaacag 
      gaaaaatca gttttgagg actttctta gcatattgt 
      ccaaggtgt cccgaggca gcgtccagg agaagctcc 
      agtgggtgt tcgggctgt acgacttga acggggacg 
      gcctcatca ccaagacgg agatggtgg acgtcgttt 
      cgagcatct atgagatgt tgggccgag cgacgcatc 
      caactgtta acgaaagct cggccaaag aacacgtag 
      aaaaaatat tccatctga tagacacga ataaggacg 
      gcgtcgtca ccatcgaag aactggtgc agtggtgct 
      ctagggatg aacatgttc ttaaatctt tagaaactt 
      tggacactg tattgtgat cgtatctgc tgtcccgtt 
      ggcactcat cttatcttg tttcgaaag taaaccccg 
      gacctcccc gaacagcct gtacatatg cagatcaat 
      gttaaaacc ccacaaacc ccacctgtc ccattgaac 
      atctcatta cgtaatttg ttacggtag ttgtaactt 
      tgcaacttt atacgccaa attttaata ttgttgtgc 
      taattacta gagtagaga aacctgcac gaaactgta 
      tataaaaag ctaacgtgg ctaattttg gggccattt 
      atatctagt ggttgtagt tacgtagaa aagactatt 
      ttgtgctaa acgaattat tgttactta aaacatgat 
      tttggtgtt tctaccgta agcatcagt attaagtcc 
      gaatagcgt gctcacatt tatttaatg acatacttt 
      aatgtaaat aaatcttcc tcttcaccc acttcgcta 
      acgaccgca gtcctcaag accgttcgc acaagaaca 
      ccactggaa attttgcgg cttagcagt tttttacct 
      aaattaaat tagttttat aaacctaaa gttactcaa 
      tattgttat aagtttgac ttgaaattc taaattcta 
      gaactgtta cttagaaaa atgcatgtg ttatattct 
      tgtaagggc ccgacccgg caaagttcg tcaatattg 
      tgaatattt cataaaatt tacagaaac ttattgtga 
      tagcatatt tactataag taattaagt cattaactg 
      tcacttaaa tttaatgat gttactaag aacaataaa 
      tattaaata agtgttgta gtcagtgtc ataataaag 
      catcttatt tatt