Status
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Gene information
General information
TC004114 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1842572..1852284
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004114
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03691

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004114 RA
      MSWILTFLV FVILAQHQA DSSGVFELR LISFDNEAG 
      KDDKGKCCS GVSGPNSEC EGMCRPRFR ICLKEYQVK 
      IDTTSPCTF GDVITTEFE TNPGSDNSQ SGFDNSIAL 
      PFPFTWPGT FSLIVEAWH GNQTSHPAV LVSRLTRQR 
      WLDVSDKWT EDVHSSNYS TLKFEYRVT CKSHYYGKG 
      CENLCRPRD DQFGHYSCS PTGERVCLA GWTGDYCTK 
      PRCLPGCDE QHGHCTKPN ECRCQSGWE GPLCDQCQR 
      YPGCMHGTC VKPWDCLCN EGWGGLFCN QDLNFCTNH 
      KPCRNGGTC FNTGQGSYT CSCPAGYTG TNCELPLHD 
      CAKTPCLNG GTCNRNSTL NICICPAGY SGPRCETSV 
      RSCDEKPCQ NGGSCTNTD SGYRCECRP GYSGPDCEY 
      QANSCNPNP CKNDGTCVE STNGFTCIC PSGFTGERC 
      ETNIDDCLG NPCQNGGTC VDKINEFRC QCVPGYVGP 
      LCQNRVDYC ITKPCANGG TCLQLINDY ECKCAPGFT 
      GKDCSEEVD ECQVEPCQH GGTCVNRVH GFECLCPVG 
      FLGRQCEEV SSSAAPSAR VSSESNLTT EHVVVIATI 
      STFVPLVVL VAVGVIVCL KQRRKREKA RADEEARLQ 
      NEQNTANSS FAKRGAAMA ADAHMIKNS WGKCTNNVM 
      SSNISSPDD CSVSNISVS ECDGFPKPT MHQVIDGRP 
      VYSLQRTRS QKQLNTEPG PRASALLAA KLHEPEYEH 
      IKRLSVMSN ASAVCGSSD PSLLKRPVE KESNGVYVI 
      EEHFLRPDA ISAGLFATE V
    CDS sequence
    • >TC004114 RA
      atgtcttgg atacttacg tttctagtc tttgtcatc 
      ttagcccag caccaggcc gattcttcc ggagtcttt 
      gaattacga ctgatatca tttgacaac gaagccggg 
      aaggacgat aaggggaag tgctgtagc ggtgtctcc 
      ggaccaaac tccgaatgc gagggaatg tgtcgtccg 
      cggtttcga atttgtctc aaggaatac caggtgaag 
      atcgacacc acgtctccg tgcaccttt ggagacgtt 
      atcacaaca gaattcgag acaaatccc ggatcggac 
      aactcacaa agcggattc gacaattcc atcgctttg 
      ccttttccc ttcacttgg ccgggaacg ttttccttg 
      attgtggaa gcgtggcac gggaaccag acatcacat 
      cctgcagta ttagtgagt cgcctgact agacagagg 
      tggctggac gtgagcgat aaatggacc gaagatgtg 
      catagttcg aactattcg accctgaaa ttcgagtac 
      agggtgact tgcaagtcg cattattac ggcaagggt 
      tgcgagaat ttgtgtcgt ccccgggac gaccaattc 
      ggccattac agttgctct cccacgggt gagcgggtt 
      tgtttggct ggatggacg ggagactac tgtaccaaa 
      cctcgatgc ctgcctggc tgtgacgag cagcatggc 
      cactgcacc aagcccaac gagtgtcgg tgtcaatcg 
      ggctgggaa ggtcctctg tgcgaccag tgccagcgg 
      taccccggg tgcatgcac ggcacttgc gtgaaaccg 
      tgggactgc ctctgcaac gagggctgg ggcggcctt 
      ttctgcaac caagatctc aatttttgc accaaccat 
      aaaccttgc cgcaacgga ggcacgtgt ttcaacacg 
      ggccaaggc agctacaca tgcagttgc cccgcaggt 
      tacacgggc accaactgc gagttaccc ttacacgat 
      tgcgccaag accccctgt ttgaatggc ggtacttgc 
      aatcgcaac agcaccctc aacatttgc atctgcccg 
      gcgggctac agcggcccg cggtgcgag acttccgtc 
      cgcagttgc gacgagaag ccgtgtcaa aacggcggc 
      tcctgcacc aacaccgac tccgggtac cggtgtgag 
      tgccgtccg ggatacagc ggacccgat tgcgagtac 
      caagccaac agttgcaat ccgaatcct tgcaaaaac 
      gatggaact tgcgtcgaa agtaccaac ggcttcacg 
      tgcatttgc ccgtcgggg ttcaccggc gagcgctgc 
      gagaccaac atcgacgac tgtctaggc aacccgtgc 
      cagaacggg ggtacttgc gtcgataaa atcaacgag 
      tttcgctgc cagtgcgtt cccggctac gtgggcccc 
      ctgtgccag aaccgcgta gactattgt atcaccaaa 
      ccttgtgcc aacggagga acttgtctg cagctgatc 
      aatgactat gagtgcaag tgcgcgccg ggtttcaca 
      ggaaaagac tgtagtgaa gaagtagac gaatgccaa 
      gttgaacct tgccagcac ggtggtact tgtgtaaac 
      cgcgtccac ggttttgag tgtctttgt ccggtcggc 
      ttcttgggc cggcagtgc gaggaagtg tcgtcgagc 
      gccgctccg agtgcgaga gtgtcctcc gagtcgaac 
      ttgacgacg gaacacgtc gtggtcatc gcgacgatc 
      tcaacattc gtaccgcta gtagtatta gtcgcagtc 
      ggcgtcatc gtctgcctg aaacaacgc cgcaaacgg 
      gagaaagcc cgggcagac gaggaagcc agactgcag 
      aacgagcaa aacaccgcc aatagcagt ttcgcgaaa 
      cggggggcg gctatggcg gcggacgcc cacatgatc 
      aaaaactcg tggggcaaa tgcaccaac aacgtgatg 
      agttcgaac atttcctcg cccgatgac tgcagtgtg 
      tccaatatt agtgttagc gaatgtgat ggcttcccc 
      aaaccgact atgcaccaa gtcatagac ggaagacca 
      gtgtacagt ctgcagaga acgcgttct caaaagcag 
      ctaaatacc gaaccggga cctcgtgct tcggcgctt 
      cttgcagcc aaactgcat gagcccgag tacgaacat 
      attaaacgt ttgtctgtg atgtcaaac gcgtcggct 
      gtgtgtggc tccagtgac ccctccctg ctcaagagg 
      ccggtagag aaggagtcc aatggcgtt tatgtgata 
      gaagaacac ttcctccgc ccggatgcg atttcagcg 
      ggtctcttc gccacggag gtgtag
    mRNA sequence
    • >TC004114 RA
      gttttaaat tacaatgta actatttta tctgcacca 
      atacagaaa atgcgcccg ttttatcgt tagctatcg 
      gatgtccct tgggaaatg ccgaaaacg ggcacgccg 
      ggtggctgc ccattcgag ggtagcaag cttgcgagt 
      gtagttgta gtataaaat acgagtagt agtgaggcc 
      gagtcgcgt aaagtgtgg agagctctg gcggaagca 
      cgcagtacc ccttagagc gctcccctc cctagacgc 
      taccacacg gccgagcta aagccggct ccgtgactg 
      tgactgttg aaagcttca gtctgcatc tgccgtccg 
      tgtttgcag cagttccgt aacgaccgc ttcgggtag 
      tcacgagtg ttggtggtg tgtcgtgtc gatatgggg 
      tgcccttcg acgtactaa ttgctaaca ccttcgtat 
      gcatgtgct gtgctgtga atccccggg agtgaggca 
      cgcatgttg ccgcctgtg actgcagtg agcggaatt 
      gactattgc ctatggcgt ggtgttgca gattacctc 
      tgaggtaac agcacaagg aagctgctg aatccctcg 
      tgagcgcaa ggagccatc accatgtct tggatactt 
      acgtttcta gtctttgtc atcttagcc cagcaccag 
      gccgattct tccggagtc tttgaatta cgactgata 
      tcatttgac aacgaagcc gggaaggac gataagggg 
      aagtgctgt agcggtgtc tccggacca aactccgaa 
      tgcgaggga atgtgtcgt ccgcggttt cgaatttgt 
      ctcaaggaa taccaggtg aagatcgac accacgtct 
      ccgtgcacc tttggagac gttatcaca acagaattc 
      gagacaaat cccggatcg gacaactca caaagcgga 
      ttcgacaat tccatcgct ttgcctttt cccttcact 
      tggccggga acgttttcc ttgattgtg gaagcgtgg 
      cacgggaac cagacatca catcctgca gtattagtg 
      agtcgcctg actagacag aggtggctg gacgtgagc 
      gataaatgg accgaagat gtgcatagt tcgaactat 
      tcgaccctg aaattcgag tacagggtg acttgcaag 
      tcgcattat tacggcaag ggttgcgag aatttgtgt 
      cgtccccgg gacgaccaa ttcggccat tacagttgc 
      tctcccacg ggtgagcgg gtttgtttg gctggatgg 
      acgggagac tactgtacc aaacctcga tgcctgcct 
      ggctgtgac gagcagcat ggccactgc accaagccc 
      aacgagtgt cggtgtcaa tcgggctgg gaaggtcct 
      ctgtgcgac cagtgccag cggtacccc gggtgcatg 
      cacggcact tgcgtgaaa ccgtgggac tgcctctgc 
      aacgagggc tggggcggc cttttctgc aaccaagat 
      ctcaatttt tgcaccaac cataaacct tgccgcaac 
      ggaggcacg tgtttcaac acgggccaa ggcagctac 
      acatgcagt tgccccgca ggttacacg ggcaccaac 
      tgcgagtta cccttacac gattgcgcc aagaccccc 
      tgtttgaat ggcggtact tgcaatcgc aacagcacc 
      ctcaacatt tgcatctgc ccggcgggc tacagcggc 
      ccgcggtgc gagacttcc gtccgcagt tgcgacgag 
      aagccgtgt caaaacggc ggctcctgc accaacacc 
      gactccggg taccggtgt gagtgccgt ccgggatac 
      agcggaccc gattgcgag taccaagcc aacagttgc 
      aatccgaat ccttgcaaa aacgatgga acttgcgtc 
      gaaagtacc aacggcttc acgtgcatt tgcccgtcg 
      gggttcacc ggcgagcgc tgcgagacc aacatcgac 
      gactgtcta ggcaacccg tgccagaac gggggtact 
      tgcgtcgat aaaatcaac gagtttcgc tgccagtgc 
      gttcccggc tacgtgggc cccctgtgc cagaaccgc 
      gtagactat tgtatcacc aaaccttgt gccaacgga 
      ggaacttgt ctgcagctg atcaatgac tatgagtgc 
      aagtgcgcg ccgggtttc acaggaaaa gactgtagt 
      gaagaagta gacgaatgc caagttgaa ccttgccag 
      cacggtggt acttgtgta aaccgcgtc cacggtttt 
      gagtgtctt tgtccggtc ggcttcttg ggccggcag 
      tgcgaggaa gtgtcgtcg agcgccgct ccgagtgcg 
      agagtgtcc tccgagtcg aacttgacg acggaacac 
      gtcgtggtc atcgcgacg atctcaaca ttcgtaccg 
      ctagtagta ttagtcgca gtcggcgtc atcgtctgc 
      ctgaaacaa cgccgcaaa cgggagaaa gcccgggca 
      gacgaggaa gccagactg cagaacgag caaaacacc 
      gccaatagc agtttcgcg aaacggggg gcggctatg 
      gcggcggac gcccacatg atcaaaaac tcgtggggc 
      aaatgcacc aacaacgtg atgagttcg aacatttcc 
      tcgcccgat gactgcagt gtgtccaat attagtgtt 
      agcgaatgt gatggcttc cccaaaccg actatgcac 
      caagtcata gacggaaga ccagtgtac agtctgcag 
      agaacgcgt tctcaaaag cagctaaat accgaaccg 
      ggacctcgt gcttcggcg cttcttgca gccaaactg 
      catgagccc gagtacgaa catattaaa cgtttgtct 
      gtgatgtca aacgcgtcg gctgtgtgt ggctccagt 
      gacccctcc ctgctcaag aggccggta gagaaggag 
      tccaatggc gtttatgtg atagaagaa cacttcctc 
      cgcccggat gcgatttca gcgggtctc ttcgccacg 
      gaggtgtag agttagttt atatgtatg actgattga 
      gtggatgtg acgccgatc ccgccgcta gcctagggc 
      acgatgtac agttatatt tattattcg ctctaattg 
      ctagcgcgc tggatcgtc gatgtaact tatggttgc 
      atgtgtact aaaatttgg gactggtgt agtcccgtg 
      tccaaggag ccttcgccc tgcccgtga cgccgcttt 
      aaacaccgc caataacga ggactagaa cttcgctcc 
      aatgattac acttttgtt tatattgta aatattacg 
      ctagaataa tttttccgt gtctctttt tttgtcatt 
      gaaggaaga ttctggtta gaaaaccaa agattttgg 
      tgtcgtaga aagcctgct ttaagggtc gggcgcggc 
      gccgactcg tcaagtagt atgtaacta atataacac 
      ttaaattgt tattgtaat atgtgatga atctaaaat 
      taactaagt cagcattgt ctacttaca tggacttat 
      ctcaatact tgtaagcat ttttttctt agtaataac 
      ttgcttccg atttgaact tctcttctt tttttcatt 
      tggagctct agtcagaag caacttaca tgatctttt 
      ataatatga tcaacgtta attagtatt agaccctgt 
      atctgatca tgataattt tgaacaaag acgtaatgt 
      tcctcttat tataactaa gtcatttgc tgttgtgtc 
      atagaacga ttgtaaaat cgttctacg acacggatc 
      taaaacact tgttgtact acttaacca cttggacaa 
      gtttaaaga cttataatt gtcaaaaag accttttgt 
      aatatatta ttgtatatt taaagcatg taaactaaa 
      tgtcatatt agaaattgt tcatgtttc ttaattaaa 
      ccttcccta gtgaagctg tattgttcc tcacgtgaa 
      tgatgttat ttaaagtta tcataataa attttttgt 
      tctcta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY