Status
Please wait ...
Gene information
General information
TC004111 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1878985..1889601
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004111
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03690

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition >5 number 2nd lateral oviduct: number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva cuticle dorsal irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004111 RA
      MAHTFRNSY EKTFKVLDN SEAKMHHEL NGSTSKPIH 
      VQRVERAVQ LNNFLKEVD YQLDENSNS DDSIDISNN 
      FDNLLRESE SHSTKDYYN LKHVSQRRA WLAEVLQEN 
      ADSTPDDAQ YKHITKLHT WQKQIRSTK DVDFKRYIY 
      YGSGLLSEI DRYPEIEKS LPVKSVKKR HIKEETSKS 
      PLYPPEILE DISDEPCIG LSLDNVIDF QNLDEDSNV 
      NSDFGQPVS QTSHQTNTS HSSSKKRVK KFKDEESRK 
      KWEDMMKVK RKKIFTNMA KKEIGKQHR SKINKHKEM 
      LIQCRRVAQ QCLKYGRQK ALQSARTVK EQQWRMKRL 
      SRENIAYWK RSRRFDREV KKRLEKEAE EQRKIDHEL 
      IEAKRQQRK LNFLITQTE LYAHFMSRK LGKASPEEQ 
      LRILSQLDE EQSSRLLNI DDYDSEVMK EKAKRNAQE 
      AFQNEKVRT RHFDIEAKS SVDVNAIDG EQPQPSIFQ 
      GKLKGYQLR GMNWLANLY AQGISGILA DEMGLGKTV 
      QSIAFLCHI AERYSVWGP FLIISPAST LHNWQQEIA 
      KFVPNFKVV PYWGNPNER KILRQFWDQ KDIYTKDAS 
      FHIVITSYQ IVITDIKYF NRIKWQYMI LDEAQAIKS 
      TSSMRWKTL LGFSCRNRL LLSGTPIQN SMAELWALL 
      HFIMPTLFD SHEEFNEWF SKDIESHAE NKTGIDEKH 
      LSRLHMILK PFMLRRIKK DVENELSDK IEVMVYCPL 
      TTRQQLLYM ALKQKIKIE DLLHYSVGG GDSHTVDKN 
      FTSNLMNLV MQFRKVCNH PELFERRDA KSPIHIAPC 
      VFRIPYLIY DFNVTAVLM LTLQKYFVF TPGYIAEAV 
      KNDFNTLFR VHSFLNLSF YELYMILQG DVRTRWTHF 
      FRSENEDEI LYHRYFWNE GSTKPSMRI SYKFKLTNA 
      NIRTSSILP ELIFTRKNS GSRVFYTHT DQYYYPMPE 
      TVEHRNIRC KNALNNEED AIIDVVADE SPLTEEVKS 
      EGGEAGTYR DETPRIQKH ALTDLPSFL FCTMPKVTT 
      IFPNLYCYS RRVAWLIRR HNYEFSYNS LNHYWSEAT 
      GHRNRKPAE TLYPQPTNA IDVLRPLKG WSYIAIPDK 
      ETLVTDSGK LSVLDGLLK RLKEEGHRV LIYSQMTKM 
      IDLLEEYMW HRHHKYMRL DGSSKISER RDMVADFQA 
      RTDIFVFLL STRAGGLGI NLTAADTVI FYDSDWNPT 
      VDQQAMDRA HRLGQTKQV TVYRLICKG SIEERILQR 
      AREKSEIQK LVISGGNFK PDTLKPKEV VSLLLDDAE 
      LIHRSEKLR VDFNKTKDE DKELEKKRK HMLAFKTIP 
      AVPELKKTK SDSSQETYI EDWSSTLDS GPSSPHSAN 
      SPDVLTDDM AESGYGEED SPITNKYTV YNVYGSTVK 
      PRIPGRGGS RRGRPRGSR RMSSLGRTN SSISSTSTN 
      GENEYSTNS QSTLASPTS NNYLNVPLG ASGSQSPNL 
      AIRRGPGRP RLKPTGPPN TGSRGTYRP RKPARPLPV 
      PLPSDGSNV SCSTSGASA TPSYSTYLY DLPDQDS
    CDS sequence
    • >TC004111 RA
      atggcacac acttttagg aacagctac gagaaaaca 
      ttcaaagta ttagataac agcgaagct aaaatgcat 
      cacgagttg aacgggagc acctccaaa cctatccat 
      gttcagcgc gttgaaagg gcggtgcaa ttaaataat 
      tttctcaaa gaggttgac tatcagttg gacgaaaat 
      agtaacagt gatgattca atcgatatt tcgaacaat 
      tttgataat ttattaaga gagtcagaa tcacattca 
      accaaagat tattataac ttgaaacat gtctcgcaa 
      aggcgggct tggttggct gaggtttta caagagaat 
      gcagacagc accccggac gatgcacaa tacaaacac 
      attacgaaa cttcacact tggcaaaaa cagatacgc 
      agtacaaaa gatgttgat tttaagcgt tatatttat 
      tacggatcc gggttatta agcgagatt gataggtat 
      ccggaaata gaaaaatca ctcccagtc aagagtgtt 
      aaaaaacgc cacattaag gaagaaacg tccaaatct 
      cccctgtac ccacccgaa attctcgaa gatatttca 
      gatgagccc tgtataggt ctgtcttta gacaacgtc 
      atagatttt caaaatctt gacgaggac tctaatgtt 
      aattccgat tttgggcaa ccagttagt caaacctca 
      catcaaaca aacacatca catagttca agtaagaag 
      cgagtaaag aagtttaaa gacgaagaa tctcggaaa 
      aagtgggaa gacatgatg aaagtcaag cgtaaaaaa 
      atattcaca aacatggcc aaaaaagaa atcgggaag 
      caacaccgt tctaaaata aacaaacac aaagagatg 
      ttgatacag tgtcggcgg gtagcccaa cagtgtctt 
      aaatacggc agacagaaa gctcttcaa tctgcccga 
      acagtcaag gaacaacag tggcggatg aaacgcctg 
      tcacgcgaa aacatagct tactggaaa cgctcgaga 
      cgattcgac cgcgaagtg aaaaaacgc ctggaaaag 
      gaagcagaa gagcagagg aaaatcgac cacgaacta 
      atcgaagcc aagcgccag caacgcaaa ctaaacttc 
      ctcatcaca cagaccgag ctttacgcc cacttcatg 
      tcgcggaag ctgggaaaa gcgtcacca gaggaacag 
      ttacggatt ttgagccag ctggacgaa gagcagagc 
      tcgcgattg ttgaacatc gacgactac gacagcgaa 
      gtgatgaaa gagaaggcg aagcgaaac gcacaagag 
      gcgtttcag aacgagaaa gtcaggact cggcatttc 
      gacattgag gcaaaatcc tcggtggac gtgaacgcc 
      atcgacggg gagcagccc cagcccagt attttccaa 
      gggaagctc aaaggctac caactgcgg ggcatgaac 
      tggctggcg aacttgtac gcccagggc ataagcggg 
      atcttggcg gatgaaatg ggcttgggc aaaacggtg 
      caaagcatc gcgtttctg tgccacatt gcggagaga 
      tactcggtt tggggacca tttcttatt atttcgccg 
      gcgtccact ctgcacaac tggcagcag gaaatcgcg 
      aaattcgtg cctaacttc aaagtcgtc ccctattgg 
      ggaaacccg aacgagagg aaaattttg cgccagttt 
      tgggatcaa aaagacatc tatacgaaa gatgccagc 
      tttcacata gtcatcacc agttatcag atcgttatc 
      acggatatt aaatatttt aataggatt aagtggcag 
      tatatgatt ctggatgaa gcccaagcg atcaaaagc 
      acaagcagc atgcggtgg aagacactt ttaggtttc 
      agttgcaga aatcgcctc ttgctgagt ggcaccccc 
      attcagaac agcatggcc gagctttgg gccttactg 
      cacttcatt atgcccaca cttttcgac tcgcacgag 
      gagtttaac gagtggttc tccaaagat atcgagagc 
      cacgccgaa aataaaact ggaattgac gaaaagcat 
      ctctcgcgc ctgcacatg attctgaag ccgttcatg 
      ctgcgccgc atcaagaaa gacgttgaa aacgagctt 
      tccgacaaa atcgaagtg atggtctac tgtcctctt 
      acgacgcgc cagcagctt ctgtacatg gcacttaaa 
      caaaaaatt aaaattgag gacctgctc cattacagt 
      gttggcggt ggcgattcg cacacagtc gacaaaaac 
      ttcacgtcc aacttgatg aatctagtt atgcaattt 
      agaaaggtg tgtaaccat ccggaatta tttgaaaga 
      agagatgcc aagtcgccg attcatata gcaccttgc 
      gtgttccga atcccgtat ttaatttat gattttaat 
      gtcaccgct gtgctcatg cttacattg caaaaatat 
      ttcgtattc acgcctgga tacatcgcg gaagcggtc 
      aagaatgat tttaatacg ctttttaga gttcatagt 
      tttttaaat ctgtcgttc tatgagttg tatatgatt 
      ttacaaggg gatgtgaga acgaggtgg acgcacttt 
      ttccgtagt gaaaatgag gacgaaatt ttatatcat 
      cgctacttc tggaatgag ggttcaacc aaaccttca 
      atgcgtatt agttataag tttaaactc actaacgct 
      aatataaga acaagtagt attttaccc gagttaatt 
      tttacgagg aaaaattca ggaagtcgt gttttttac 
      acacacact gatcagtat tattacccc atgccggaa 
      acagtcgaa cataggaat attaggtgt aagaacgcg 
      ctcaataat gaggaagat gccattatt gacgtagtg 
      gcggacgag tctcctctc accgaggaa gttaaatcg 
      gaaggtggt gaagctggc acctacaga gacgaaaca 
      cctcgcata caaaaacat gcgctaact gacttgcct 
      tcattttta ttctgcacg atgcccaag gtgacaacc 
      atcttccct aatctatat tgttattca cgacgagtc 
      gcttggcta atccgacgt cacaattac gagtttagt 
      tacaattct cttaatcac tattggtcg gaagccacc 
      ggccacagg aatcgcaaa ccggcggaa actttatac 
      cctcaacca accaatgct attgacgtt ctcaggcct 
      ttaaaaggc tggtcctac attgcaatc ccagataag 
      gaaacgcta gtgactgat tcgggaaaa ctctcggtg 
      cttgacggc ctcctcaag cgcctgaag gaggaaggc 
      caccgagtg ttgatttac tcccaaatg acgaaaatg 
      atcgacttg ctggaagaa tatatgtgg caccggcac 
      cataaatac atgcgtctt gacggttct tcaaaaata 
      tcagagaga cgtgatatg gttgcagat ttccaggcc 
      aggaccgat atatttgtt ttcctgctc tccacacgg 
      gccggcggt ttgggaatt aacctgacg gctgctgat 
      accgtgata ttttacgat agcgattgg aacccaacg 
      gttgaccaa caagccatg gaccgagct caccgtctc 
      gggcaaact aaacaagtc actgtgtat agacttatt 
      tgcaaaggg tcaatcgaa gagcgaatc cttcaaagg 
      gctagagag aaaagcgaa atccaaaaa ttagtcatt 
      agtggaggc aatttcaag cccgacact ctaaaacca 
      aaagaagtc gtttcttta ctcttggat gacgctgaa 
      ttgattcat agatcggaa aagttgaga gttgacttt 
      aacaaaacg aaagacgaa gacaaggag ctggaaaag 
      aagagaaaa catatgctt gcttttaag acgattcct 
      gccgtcccc gagctgaaa aaaaccaag tcggacagc 
      agccaagag acgtacata gaagactgg agcagcact 
      ctggacagt ggtccaagc agcccccac agtgcgaat 
      tctccggac gttttaaca gacgatatg gcggagagc 
      ggctacggc gaggaggac tctccaatt actaacaaa 
      tacactgtt tataacgtg tatggttca acggtaaag 
      ccgcggatt ccgggacga ggaggatcg agacgggga 
      cgacctcgc ggttcgcga aggatgtca agcctgggc 
      cgaacaaac agcagcatt tcatccacg agcaccaac 
      ggtgaaaat gagtattca acgaacagc caatcaact 
      ttggcttcg ccaacttcg aacaattac ctaaacgtc 
      cctcttgga gcgagtgga agtcagtcg ccaaacctt 
      gcgatacgg cgcggcccc gggcgtccg aggctcaag 
      ccaacagga ccgccaaac acaggatca agagggacg 
      tacagaccg aggaaacct gcgagacct ttgccagtc 
      ccactgcca tctgacggt tcaaatgtc agttgttcg 
      acttctgga gccagtgcg acaccttct tattctacc 
      tacctttac gaccttccg gatcaagat tcatag
    mRNA sequence
    • >TC004111 RA
      agtagttga tttgttttg ataccaacc accgcaatg 
      tgaggttat gtgataaaa atatataat taaatggca 
      ttcactgta ggaattatg aaagtatta gtgaagtaa 
      cgtttgttt taaccgaaa taacacacc ctccagcca 
      tggcacaca cttttagga acagctacg agaaaacat 
      tcaaagtat tagataaca gcgaagcta aaatgcatc 
      acgagttga acgggagca cctccaaac ctatccatg 
      ttcagcgcg ttgaaaggg cggtgcaat taaataatt 
      ttctcaaag aggttgact atcagttgg acgaaaata 
      gtaacagtg atgattcaa tcgatattt cgaacaatt 
      ttgataatt tattaagag agtcagaat cacattcaa 
      ccaaagatt attataact tgaaacatg tctcgcaaa 
      ggcgggctt ggttggctg aggttttac aagagaatg 
      cagacagca ccccggacg atgcacaat acaaacaca 
      ttacgaaac ttcacactt ggcaaaaac agatacgca 
      gtacaaaag atgttgatt ttaagcgtt atatttatt 
      acggatccg ggttattaa gcgagattg ataggtatc 
      cggaaatag aaaaatcac tcccagtca agagtgtta 
      aaaaacgcc acattaagg aagaaacgt ccaaatctc 
      ccctgtacc cacccgaaa ttctcgaag atatttcag 
      atgagccct gtataggtc tgtctttag acaacgtca 
      tagattttc aaaatcttg acgaggact ctaatgtta 
      attccgatt ttgggcaac cagttagtc aaacctcac 
      atcaaacaa acacatcac atagttcaa gtaagaagc 
      gagtaaaga agtttaaag acgaagaat ctcggaaaa 
      agtgggaag acatgatga aagtcaagc gtaaaaaaa 
      tattcacaa acatggcca aaaaagaaa tcgggaagc 
      aacaccgtt ctaaaataa acaaacaca aagagatgt 
      tgatacagt gtcggcggg tagcccaac agtgtctta 
      aatacggca gacagaaag ctcttcaat ctgcccgaa 
      cagtcaagg aacaacagt ggcggatga aacgcctgt 
      cacgcgaaa acatagctt actggaaac gctcgagac 
      gattcgacc gcgaagtga aaaaacgcc tggaaaagg 
      aagcagaag agcagagga aaatcgacc acgaactaa 
      tcgaagcca agcgccagc aacgcaaac taaacttcc 
      tcatcacac agaccgagc tttacgccc acttcatgt 
      cgcggaagc tgggaaaag cgtcaccag aggaacagt 
      tacggattt tgagccagc tggacgaag agcagagct 
      cgcgattgt tgaacatcg acgactacg acagcgaag 
      tgatgaaag agaaggcga agcgaaacg cacaagagg 
      cgtttcaga acgagaaag tcaggactc ggcatttcg 
      acattgagg caaaatcct cggtggacg tgaacgcca 
      tcgacgggg agcagcccc agcccagta ttttccaag 
      ggaagctca aaggctacc aactgcggg gcatgaact 
      ggctggcga acttgtacg cccagggca taagcggga 
      tcttggcgg atgaaatgg gcttgggca aaacggtgc 
      aaagcatcg cgtttctgt gccacattg cggagagat 
      actcggttt ggggaccat ttcttatta tttcgccgg 
      cgtccactc tgcacaact ggcagcagg aaatcgcga 
      aattcgtgc ctaacttca aagtcgtcc cctattggg 
      gaaacccga acgagagga aaattttgc gccagtttt 
      gggatcaaa aagacatct atacgaaag atgccagct 
      ttcacatag tcatcacca gttatcaga tcgttatca 
      cggatatta aatatttta ataggatta agtggcagt 
      atatgattc tggatgaag cccaagcga tcaaaagca 
      caagcagca tgcggtgga agacacttt taggtttca 
      gttgcagaa atcgcctct tgctgagtg gcaccccca 
      ttcagaaca gcatggccg agctttggg ccttactgc 
      acttcatta tgcccacac ttttcgact cgcacgagg 
      agtttaacg agtggttct ccaaagata tcgagagcc 
      acgccgaaa ataaaactg gaattgacg aaaagcatc 
      tctcgcgcc tgcacatga ttctgaagc cgttcatgc 
      tgcgccgca tcaagaaag acgttgaaa acgagcttt 
      ccgacaaaa tcgaagtga tggtctact gtcctctta 
      cgacgcgcc agcagcttc tgtacatgg cacttaaac 
      aaaaaatta aaattgagg acctgctcc attacagtg 
      ttggcggtg gcgattcgc acacagtcg acaaaaact 
      tcacgtcca acttgatga atctagtta tgcaattta 
      gaaaggtgt gtaaccatc cggaattat ttgaaagaa 
      gagatgcca agtcgccga ttcatatag caccttgcg 
      tgttccgaa tcccgtatt taatttatg attttaatg 
      tcaccgctg tgctcatgc ttacattgc aaaaatatt 
      tcgtattca cgcctggat acatcgcgg aagcggtca 
      agaatgatt ttaatacgc tttttagag ttcatagtt 
      ttttaaatc tgtcgttct atgagttgt atatgattt 
      tacaagggg atgtgagaa cgaggtgga cgcactttt 
      tccgtagtg aaaatgagg acgaaattt tatatcatc 
      gctacttct ggaatgagg gttcaacca aaccttcaa 
      tgcgtatta gttataagt ttaaactca ctaacgcta 
      atataagaa caagtagta ttttacccg agttaattt 
      ttacgagga aaaattcag gaagtcgtg ttttttaca 
      cacacactg atcagtatt attacccca tgccggaaa 
      cagtcgaac ataggaata ttaggtgta agaacgcgc 
      tcaataatg aggaagatg ccattattg acgtagtgg 
      cggacgagt ctcctctca ccgaggaag ttaaatcgg 
      aaggtggtg aagctggca cctacagag acgaaacac 
      ctcgcatac aaaaacatg cgctaactg acttgcctt 
      catttttat tctgcacga tgcccaagg tgacaacca 
      tcttcccta atctatatt gttattcac gacgagtcg 
      cttggctaa tccgacgtc acaattacg agtttagtt 
      acaattctc ttaatcact attggtcgg aagccaccg 
      gccacagga atcgcaaac cggcggaaa ctttatacc 
      ctcaaccaa ccaatgcta ttgacgttc tcaggcctt 
      taaaaggct ggtcctaca ttgcaatcc cagataagg 
      aaacgctag tgactgatt cgggaaaac tctcggtgc 
      ttgacggcc tcctcaagc gcctgaagg aggaaggcc 
      accgagtgt tgatttact cccaaatga cgaaaatga 
      tcgacttgc tggaagaat atatgtggc accggcacc 
      ataaataca tgcgtcttg acggttctt caaaaatat 
      cagagagac gtgatatgg ttgcagatt tccaggcca 
      ggaccgata tatttgttt tcctgctct ccacacggg 
      ccggcggtt tgggaatta acctgacgg ctgctgata 
      ccgtgatat tttacgata gcgattgga acccaacgg 
      ttgaccaac aagccatgg accgagctc accgtctcg 
      ggcaaacta aacaagtca ctgtgtata gacttattt 
      gcaaagggt caatcgaag agcgaatcc ttcaaaggg 
      ctagagaga aaagcgaaa tccaaaaat tagtcatta 
      gtggaggca atttcaagc ccgacactc taaaaccaa 
      aagaagtcg tttctttac tcttggatg acgctgaat 
      tgattcata gatcggaaa agttgagag ttgacttta 
      acaaaacga aagacgaag acaaggagc tggaaaaga 
      agagaaaac atatgcttg cttttaaga cgattcctg 
      ccgtccccg agctgaaaa aaaccaagt cggacagca 
      gccaagaga cgtacatag aagactgga gcagcactc 
      tggacagtg gtccaagca gcccccaca gtgcgaatt 
      ctccggacg ttttaacag acgatatgg cggagagcg 
      gctacggcg aggaggact ctccaatta ctaacaaat 
      acactgttt ataacgtgt atggttcaa cggtaaagc 
      cgcggattc cgggacgag gaggatcga gacggggac 
      gacctcgcg gttcgcgaa ggatgtcaa gcctgggcc 
      gaacaaaca gcagcattt catccacga gcaccaacg 
      gtgaaaatg agtattcaa cgaacagcc aatcaactt 
      tggcttcgc caacttcga acaattacc taaacgtcc 
      ctcttggag cgagtggaa gtcagtcgc caaaccttg 
      cgatacggc gcggccccg ggcgtccga ggctcaagc 
      caacaggac cgccaaaca caggatcaa gagggacgt 
      acagaccga ggaaacctg cgagacctt tgccagtcc 
      cactgccat ctgacggtt caaatgtca gttgttcga 
      cttctggag ccagtgcga caccttctt attctacct 
      acctttacg accttccgg atcaagatt catagtcgt 
      atattttta tcagacgtc gtcttttta acagaatag 
      ctgctatag taattttat gacatattt aattttaaa 
      taaaatctg gatgaagtg atctattta tgatgttaa 
      tttaataaa aacttcaca aatga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY