Status
Please wait ...
Gene information
General information
TC004111 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1878985..1889601
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004111
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03690

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Fertility
    mature egg deposition >5 number 2nd lateral oviduct: number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva cuticle dorsal irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004111 RA
      MAHTFRNSY EKTFKVLDN SEAKMHHEL NGSTSKPIH 
VQRVERAVQ LNNFLKEVD YQLDENSNS DDSIDISNN 
FDNLLRESE SHSTKDYYN LKHVSQRRA WLAEVLQEN 
ADSTPDDAQ YKHITKLHT WQKQIRSTK DVDFKRYIY 
YGSGLLSEI DRYPEIEKS LPVKSVKKR HIKEETSKS 
PLYPPEILE DISDEPCIG LSLDNVIDF QNLDEDSNV 
NSDFGQPVS QTSHQTNTS HSSSKKRVK KFKDEESRK 
KWEDMMKVK RKKIFTNMA KKEIGKQHR SKINKHKEM 
LIQCRRVAQ QCLKYGRQK ALQSARTVK EQQWRMKRL 
SRENIAYWK RSRRFDREV KKRLEKEAE EQRKIDHEL 
IEAKRQQRK LNFLITQTE LYAHFMSRK LGKASPEEQ 
LRILSQLDE EQSSRLLNI DDYDSEVMK EKAKRNAQE 
AFQNEKVRT RHFDIEAKS SVDVNAIDG EQPQPSIFQ 
GKLKGYQLR GMNWLANLY AQGISGILA DEMGLGKTV 
QSIAFLCHI AERYSVWGP FLIISPAST LHNWQQEIA 
KFVPNFKVV PYWGNPNER KILRQFWDQ KDIYTKDAS 
FHIVITSYQ IVITDIKYF NRIKWQYMI LDEAQAIKS 
TSSMRWKTL LGFSCRNRL LLSGTPIQN SMAELWALL 
HFIMPTLFD SHEEFNEWF SKDIESHAE NKTGIDEKH 
LSRLHMILK PFMLRRIKK DVENELSDK IEVMVYCPL 
TTRQQLLYM ALKQKIKIE DLLHYSVGG GDSHTVDKN 
FTSNLMNLV MQFRKVCNH PELFERRDA KSPIHIAPC 
VFRIPYLIY DFNVTAVLM LTLQKYFVF TPGYIAEAV 
KNDFNTLFR VHSFLNLSF YELYMILQG DVRTRWTHF 
FRSENEDEI LYHRYFWNE GSTKPSMRI SYKFKLTNA 
NIRTSSILP ELIFTRKNS GSRVFYTHT DQYYYPMPE 
TVEHRNIRC KNALNNEED AIIDVVADE SPLTEEVKS 
EGGEAGTYR DETPRIQKH ALTDLPSFL FCTMPKVTT 
IFPNLYCYS RRVAWLIRR HNYEFSYNS LNHYWSEAT 
GHRNRKPAE TLYPQPTNA IDVLRPLKG WSYIAIPDK 
ETLVTDSGK LSVLDGLLK RLKEEGHRV LIYSQMTKM 
IDLLEEYMW HRHHKYMRL DGSSKISER RDMVADFQA 
RTDIFVFLL STRAGGLGI NLTAADTVI FYDSDWNPT 
VDQQAMDRA HRLGQTKQV TVYRLICKG SIEERILQR 
AREKSEIQK LVISGGNFK PDTLKPKEV VSLLLDDAE 
LIHRSEKLR VDFNKTKDE DKELEKKRK HMLAFKTIP 
AVPELKKTK SDSSQETYI EDWSSTLDS GPSSPHSAN 
SPDVLTDDM AESGYGEED SPITNKYTV YNVYGSTVK 
PRIPGRGGS RRGRPRGSR RMSSLGRTN SSISSTSTN 
GENEYSTNS QSTLASPTS NNYLNVPLG ASGSQSPNL 
AIRRGPGRP RLKPTGPPN TGSRGTYRP RKPARPLPV 
PLPSDGSNV SCSTSGASA TPSYSTYLY DLPDQDS
    CDS sequence
    • >TC004111 RA
      atggcacac acttttagg aacagctac gagaaaaca 
ttcaaagta ttagataac agcgaagct aaaatgcat 
cacgagttg aacgggagc acctccaaa cctatccat 
gttcagcgc gttgaaagg gcggtgcaa ttaaataat 
tttctcaaa gaggttgac tatcagttg gacgaaaat 
agtaacagt gatgattca atcgatatt tcgaacaat 
tttgataat ttattaaga gagtcagaa tcacattca 
accaaagat tattataac ttgaaacat gtctcgcaa 
aggcgggct tggttggct gaggtttta caagagaat 
gcagacagc accccggac gatgcacaa tacaaacac 
attacgaaa cttcacact tggcaaaaa cagatacgc 
agtacaaaa gatgttgat tttaagcgt tatatttat 
tacggatcc gggttatta agcgagatt gataggtat 
ccggaaata gaaaaatca ctcccagtc aagagtgtt 
aaaaaacgc cacattaag gaagaaacg tccaaatct 
cccctgtac ccacccgaa attctcgaa gatatttca 
gatgagccc tgtataggt ctgtcttta gacaacgtc 
atagatttt caaaatctt gacgaggac tctaatgtt 
aattccgat tttgggcaa ccagttagt caaacctca 
catcaaaca aacacatca catagttca agtaagaag 
cgagtaaag aagtttaaa gacgaagaa tctcggaaa 
aagtgggaa gacatgatg aaagtcaag cgtaaaaaa 
atattcaca aacatggcc aaaaaagaa atcgggaag 
caacaccgt tctaaaata aacaaacac aaagagatg 
ttgatacag tgtcggcgg gtagcccaa cagtgtctt 
aaatacggc agacagaaa gctcttcaa tctgcccga 
acagtcaag gaacaacag tggcggatg aaacgcctg 
tcacgcgaa aacatagct tactggaaa cgctcgaga 
cgattcgac cgcgaagtg aaaaaacgc ctggaaaag 
gaagcagaa gagcagagg aaaatcgac cacgaacta 
atcgaagcc aagcgccag caacgcaaa ctaaacttc 
ctcatcaca cagaccgag ctttacgcc cacttcatg 
tcgcggaag ctgggaaaa gcgtcacca gaggaacag 
ttacggatt ttgagccag ctggacgaa gagcagagc 
tcgcgattg ttgaacatc gacgactac gacagcgaa 
gtgatgaaa gagaaggcg aagcgaaac gcacaagag 
gcgtttcag aacgagaaa gtcaggact cggcatttc 
gacattgag gcaaaatcc tcggtggac gtgaacgcc 
atcgacggg gagcagccc cagcccagt attttccaa 
gggaagctc aaaggctac caactgcgg ggcatgaac 
tggctggcg aacttgtac gcccagggc ataagcggg 
atcttggcg gatgaaatg ggcttgggc aaaacggtg 
caaagcatc gcgtttctg tgccacatt gcggagaga 
tactcggtt tggggacca tttcttatt atttcgccg 
gcgtccact ctgcacaac tggcagcag gaaatcgcg 
aaattcgtg cctaacttc aaagtcgtc ccctattgg 
ggaaacccg aacgagagg aaaattttg cgccagttt 
tgggatcaa aaagacatc tatacgaaa gatgccagc 
tttcacata gtcatcacc agttatcag atcgttatc 
acggatatt aaatatttt aataggatt aagtggcag 
tatatgatt ctggatgaa gcccaagcg atcaaaagc 
acaagcagc atgcggtgg aagacactt ttaggtttc 
agttgcaga aatcgcctc ttgctgagt ggcaccccc 
attcagaac agcatggcc gagctttgg gccttactg 
cacttcatt atgcccaca cttttcgac tcgcacgag 
gagtttaac gagtggttc tccaaagat atcgagagc 
cacgccgaa aataaaact ggaattgac gaaaagcat 
ctctcgcgc ctgcacatg attctgaag ccgttcatg 
ctgcgccgc atcaagaaa gacgttgaa aacgagctt 
tccgacaaa atcgaagtg atggtctac tgtcctctt 
acgacgcgc cagcagctt ctgtacatg gcacttaaa 
caaaaaatt aaaattgag gacctgctc cattacagt 
gttggcggt ggcgattcg cacacagtc gacaaaaac 
ttcacgtcc aacttgatg aatctagtt atgcaattt 
agaaaggtg tgtaaccat ccggaatta tttgaaaga 
agagatgcc aagtcgccg attcatata gcaccttgc 
gtgttccga atcccgtat ttaatttat gattttaat 
gtcaccgct gtgctcatg cttacattg caaaaatat 
ttcgtattc acgcctgga tacatcgcg gaagcggtc 
aagaatgat tttaatacg ctttttaga gttcatagt 
tttttaaat ctgtcgttc tatgagttg tatatgatt 
ttacaaggg gatgtgaga acgaggtgg acgcacttt 
ttccgtagt gaaaatgag gacgaaatt ttatatcat 
cgctacttc tggaatgag ggttcaacc aaaccttca 
atgcgtatt agttataag tttaaactc actaacgct 
aatataaga acaagtagt attttaccc gagttaatt 
tttacgagg aaaaattca ggaagtcgt gttttttac 
acacacact gatcagtat tattacccc atgccggaa 
acagtcgaa cataggaat attaggtgt aagaacgcg 
ctcaataat gaggaagat gccattatt gacgtagtg 
gcggacgag tctcctctc accgaggaa gttaaatcg 
gaaggtggt gaagctggc acctacaga gacgaaaca 
cctcgcata caaaaacat gcgctaact gacttgcct 
tcattttta ttctgcacg atgcccaag gtgacaacc 
atcttccct aatctatat tgttattca cgacgagtc 
gcttggcta atccgacgt cacaattac gagtttagt 
tacaattct cttaatcac tattggtcg gaagccacc 
ggccacagg aatcgcaaa ccggcggaa actttatac 
cctcaacca accaatgct attgacgtt ctcaggcct 
ttaaaaggc tggtcctac attgcaatc ccagataag 
gaaacgcta gtgactgat tcgggaaaa ctctcggtg 
cttgacggc ctcctcaag cgcctgaag gaggaaggc 
caccgagtg ttgatttac tcccaaatg acgaaaatg 
atcgacttg ctggaagaa tatatgtgg caccggcac 
cataaatac atgcgtctt gacggttct tcaaaaata 
tcagagaga cgtgatatg gttgcagat ttccaggcc 
aggaccgat atatttgtt ttcctgctc tccacacgg 
gccggcggt ttgggaatt aacctgacg gctgctgat 
accgtgata ttttacgat agcgattgg aacccaacg 
gttgaccaa caagccatg gaccgagct caccgtctc 
gggcaaact aaacaagtc actgtgtat agacttatt 
tgcaaaggg tcaatcgaa gagcgaatc cttcaaagg 
gctagagag aaaagcgaa atccaaaaa ttagtcatt 
agtggaggc aatttcaag cccgacact ctaaaacca 
aaagaagtc gtttcttta ctcttggat gacgctgaa 
ttgattcat agatcggaa aagttgaga gttgacttt 
aacaaaacg aaagacgaa gacaaggag ctggaaaag 
aagagaaaa catatgctt gcttttaag acgattcct 
gccgtcccc gagctgaaa aaaaccaag tcggacagc 
agccaagag acgtacata gaagactgg agcagcact 
ctggacagt ggtccaagc agcccccac agtgcgaat 
tctccggac gttttaaca gacgatatg gcggagagc 
ggctacggc gaggaggac tctccaatt actaacaaa 
tacactgtt tataacgtg tatggttca acggtaaag 
ccgcggatt ccgggacga ggaggatcg agacgggga 
cgacctcgc ggttcgcga aggatgtca agcctgggc 
cgaacaaac agcagcatt tcatccacg agcaccaac 
ggtgaaaat gagtattca acgaacagc caatcaact 
ttggcttcg ccaacttcg aacaattac ctaaacgtc 
cctcttgga gcgagtgga agtcagtcg ccaaacctt 
gcgatacgg cgcggcccc gggcgtccg aggctcaag 
ccaacagga ccgccaaac acaggatca agagggacg 
tacagaccg aggaaacct gcgagacct ttgccagtc 
ccactgcca tctgacggt tcaaatgtc agttgttcg 
acttctgga gccagtgcg acaccttct tattctacc 
tacctttac gaccttccg gatcaagat tcatag
    mRNA sequence
    • >TC004111 RA
      agtagttga tttgttttg ataccaacc accgcaatg 
tgaggttat gtgataaaa atatataat taaatggca 
ttcactgta ggaattatg aaagtatta gtgaagtaa 
cgtttgttt taaccgaaa taacacacc ctccagcca 
tggcacaca cttttagga acagctacg agaaaacat 
tcaaagtat tagataaca gcgaagcta aaatgcatc 
acgagttga acgggagca cctccaaac ctatccatg 
ttcagcgcg ttgaaaggg cggtgcaat taaataatt 
ttctcaaag aggttgact atcagttgg acgaaaata 
gtaacagtg atgattcaa tcgatattt cgaacaatt 
ttgataatt tattaagag agtcagaat cacattcaa 
ccaaagatt attataact tgaaacatg tctcgcaaa 
ggcgggctt ggttggctg aggttttac aagagaatg 
cagacagca ccccggacg atgcacaat acaaacaca 
ttacgaaac ttcacactt ggcaaaaac agatacgca 
gtacaaaag atgttgatt ttaagcgtt atatttatt 
acggatccg ggttattaa gcgagattg ataggtatc 
cggaaatag aaaaatcac tcccagtca agagtgtta 
aaaaacgcc acattaagg aagaaacgt ccaaatctc 
ccctgtacc cacccgaaa ttctcgaag atatttcag 
atgagccct gtataggtc tgtctttag acaacgtca 
tagattttc aaaatcttg acgaggact ctaatgtta 
attccgatt ttgggcaac cagttagtc aaacctcac 
atcaaacaa acacatcac atagttcaa gtaagaagc 
gagtaaaga agtttaaag acgaagaat ctcggaaaa 
agtgggaag acatgatga aagtcaagc gtaaaaaaa 
tattcacaa acatggcca aaaaagaaa tcgggaagc 
aacaccgtt ctaaaataa acaaacaca aagagatgt 
tgatacagt gtcggcggg tagcccaac agtgtctta 
aatacggca gacagaaag ctcttcaat ctgcccgaa 
cagtcaagg aacaacagt ggcggatga aacgcctgt 
cacgcgaaa acatagctt actggaaac gctcgagac 
gattcgacc gcgaagtga aaaaacgcc tggaaaagg 
aagcagaag agcagagga aaatcgacc acgaactaa 
tcgaagcca agcgccagc aacgcaaac taaacttcc 
tcatcacac agaccgagc tttacgccc acttcatgt 
cgcggaagc tgggaaaag cgtcaccag aggaacagt 
tacggattt tgagccagc tggacgaag agcagagct 
cgcgattgt tgaacatcg acgactacg acagcgaag 
tgatgaaag agaaggcga agcgaaacg cacaagagg 
cgtttcaga acgagaaag tcaggactc ggcatttcg 
acattgagg caaaatcct cggtggacg tgaacgcca 
tcgacgggg agcagcccc agcccagta ttttccaag 
ggaagctca aaggctacc aactgcggg gcatgaact 
ggctggcga acttgtacg cccagggca taagcggga 
tcttggcgg atgaaatgg gcttgggca aaacggtgc 
aaagcatcg cgtttctgt gccacattg cggagagat 
actcggttt ggggaccat ttcttatta tttcgccgg 
cgtccactc tgcacaact ggcagcagg aaatcgcga 
aattcgtgc ctaacttca aagtcgtcc cctattggg 
gaaacccga acgagagga aaattttgc gccagtttt 
gggatcaaa aagacatct atacgaaag atgccagct 
ttcacatag tcatcacca gttatcaga tcgttatca 
cggatatta aatatttta ataggatta agtggcagt 
atatgattc tggatgaag cccaagcga tcaaaagca 
caagcagca tgcggtgga agacacttt taggtttca 
gttgcagaa atcgcctct tgctgagtg gcaccccca 
ttcagaaca gcatggccg agctttggg ccttactgc 
acttcatta tgcccacac ttttcgact cgcacgagg 
agtttaacg agtggttct ccaaagata tcgagagcc 
acgccgaaa ataaaactg gaattgacg aaaagcatc 
tctcgcgcc tgcacatga ttctgaagc cgttcatgc 
tgcgccgca tcaagaaag acgttgaaa acgagcttt 
ccgacaaaa tcgaagtga tggtctact gtcctctta 
cgacgcgcc agcagcttc tgtacatgg cacttaaac 
aaaaaatta aaattgagg acctgctcc attacagtg 
ttggcggtg gcgattcgc acacagtcg acaaaaact 
tcacgtcca acttgatga atctagtta tgcaattta 
gaaaggtgt gtaaccatc cggaattat ttgaaagaa 
gagatgcca agtcgccga ttcatatag caccttgcg 
tgttccgaa tcccgtatt taatttatg attttaatg 
tcaccgctg tgctcatgc ttacattgc aaaaatatt 
tcgtattca cgcctggat acatcgcgg aagcggtca 
agaatgatt ttaatacgc tttttagag ttcatagtt 
ttttaaatc tgtcgttct atgagttgt atatgattt 
tacaagggg atgtgagaa cgaggtgga cgcactttt 
tccgtagtg aaaatgagg acgaaattt tatatcatc 
gctacttct ggaatgagg gttcaacca aaccttcaa 
tgcgtatta gttataagt ttaaactca ctaacgcta 
atataagaa caagtagta ttttacccg agttaattt 
ttacgagga aaaattcag gaagtcgtg ttttttaca 
cacacactg atcagtatt attacccca tgccggaaa 
cagtcgaac ataggaata ttaggtgta agaacgcgc 
tcaataatg aggaagatg ccattattg acgtagtgg 
cggacgagt ctcctctca ccgaggaag ttaaatcgg 
aaggtggtg aagctggca cctacagag acgaaacac 
ctcgcatac aaaaacatg cgctaactg acttgcctt 
catttttat tctgcacga tgcccaagg tgacaacca 
tcttcccta atctatatt gttattcac gacgagtcg 
cttggctaa tccgacgtc acaattacg agtttagtt 
acaattctc ttaatcact attggtcgg aagccaccg 
gccacagga atcgcaaac cggcggaaa ctttatacc 
ctcaaccaa ccaatgcta ttgacgttc tcaggcctt 
taaaaggct ggtcctaca ttgcaatcc cagataagg 
aaacgctag tgactgatt cgggaaaac tctcggtgc 
ttgacggcc tcctcaagc gcctgaagg aggaaggcc 
accgagtgt tgatttact cccaaatga cgaaaatga 
tcgacttgc tggaagaat atatgtggc accggcacc 
ataaataca tgcgtcttg acggttctt caaaaatat 
cagagagac gtgatatgg ttgcagatt tccaggcca 
ggaccgata tatttgttt tcctgctct ccacacggg 
ccggcggtt tgggaatta acctgacgg ctgctgata 
ccgtgatat tttacgata gcgattgga acccaacgg 
ttgaccaac aagccatgg accgagctc accgtctcg 
ggcaaacta aacaagtca ctgtgtata gacttattt 
gcaaagggt caatcgaag agcgaatcc ttcaaaggg 
ctagagaga aaagcgaaa tccaaaaat tagtcatta 
gtggaggca atttcaagc ccgacactc taaaaccaa 
aagaagtcg tttctttac tcttggatg acgctgaat 
tgattcata gatcggaaa agttgagag ttgacttta 
acaaaacga aagacgaag acaaggagc tggaaaaga 
agagaaaac atatgcttg cttttaaga cgattcctg 
ccgtccccg agctgaaaa aaaccaagt cggacagca 
gccaagaga cgtacatag aagactgga gcagcactc 
tggacagtg gtccaagca gcccccaca gtgcgaatt 
ctccggacg ttttaacag acgatatgg cggagagcg 
gctacggcg aggaggact ctccaatta ctaacaaat 
acactgttt ataacgtgt atggttcaa cggtaaagc 
cgcggattc cgggacgag gaggatcga gacggggac 
gacctcgcg gttcgcgaa ggatgtcaa gcctgggcc 
gaacaaaca gcagcattt catccacga gcaccaacg 
gtgaaaatg agtattcaa cgaacagcc aatcaactt 
tggcttcgc caacttcga acaattacc taaacgtcc 
ctcttggag cgagtggaa gtcagtcgc caaaccttg 
cgatacggc gcggccccg ggcgtccga ggctcaagc 
caacaggac cgccaaaca caggatcaa gagggacgt 
acagaccga ggaaacctg cgagacctt tgccagtcc 
cactgccat ctgacggtt caaatgtca gttgttcga 
cttctggag ccagtgcga caccttctt attctacct 
acctttacg accttccgg atcaagatt catagtcgt 
atattttta tcagacgtc gtcttttta acagaatag 
ctgctatag taattttat gacatattt aattttaaa 
taaaatctg gatgaagtg atctattta tgatgttaa 
tttaataaa aacttcaca aatga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY