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Gene information
General information
TC004062 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid1574: 72429..74115
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004062
    iBeetle screen iB_03682
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004062 RA
      MSEEPPHCN HFVIRKKRF CRMTVKPGD TFCGEHQPA 
      GATTDDKIR IVCPLDPKH TCYAHNLRK HLKICNARP 
      GAPVPYLQP GVNSGGAPE DHKLLSEFA PSDIVAVIG 
      KINTIFEDC LRGKITSKL GGHKVVEAE MAKPEYGDK 
      SKKHLKQAS AILGLLDES GLIKPETCF VEFGAGRGL 
      LSYWLAQAC PQATFLLIE RSSPKHKRD NKLEKSDKV 
      RRIRADIAD LVLDKLDAV SGNPIVGVT KHLCGDATD 
      LALRCLANV SSVKVGGLT MTFCCHHRC RWPSYVGKD 
      FFNRFGLTK NDFEMMCGM SSWATCGTG FSREKNQTG 
      GTVEGNDRD KEIGVTRSQ KEELGRRSK AILNWGRLQ 
      FLEGLGFEC RLHYYVEAD VSLENVCIV AKK
    CDS sequence
    • >TC004062 RA
      atgagtgag gagcccccc cattgcaac cactttgtg 
      attcgtaaa aagcgcttt tgtcgcatg accgtcaag 
      ccgggtgac acgttttgc ggggagcac caaccggct 
      ggggccaca acggacgat aaaatccga attgtgtgc 
      ccccttgac cctaaacac acgtgttac gcccataat 
      ttgcgcaaa cacttgaaa atttgcaac gcgcggccg 
      ggggccccc gtgccctac ctccagccg ggggtgaat 
      tcaggtggg gcacccgag gaccataaa ctcctgtcg 
      gagtttgcc cccagtgac attgttgca gttattggg 
      aaaattaat acgattttt gaggattgt ttaaggggg 
      aaaattaca agtaagtta ggggggcac aaagtggtg 
      gaggctgaa atggcaaag cccgagtat ggggacaag 
      agcaagaaa cacctaaaa caggcgtcg gcgattttg 
      gggttgttg gacgagagt ggtttgatt aaacccgaa 
      acgtgtttt gtggagttt ggggctggg agggggctg 
      ttgagttat tggttggct caggcgtgc ccccaggcc 
      acttttctc ttaatcgag cggtcttcc cccaaacac 
      aagcgtgat aataaattg gaaaaaagc gataaagtc 
      cgaaggatt cgggcggac attgccgac ttggtttta 
      gataagttg gacgctgtt tcggggaat cccattgtt 
      ggggtcacg aagcatttg tgcggcgac gccactgat 
      ttggccttg agatgtttg gccaacgtt tcaagcgtt 
      aaggtaggg ggtctcact atgactttt tgttgccac 
      cataggtgt cgctggccg agctatgtc ggcaaagat 
      ttttttaat cgatttggt ttaacaaaa aatgatttt 
      gaaatgatg tgtggtatg agcagttgg gccacttgt 
      ggcactggc tttagccgg gagaagaac caaactggg 
      ggcactgtg gagggaaat gacagagac aaggagatt 
      ggagtcacc agaagccag aaggaggag ttagggagg 
      aggtcaaaa gcaatccta aattggggg cgcctccaa 
      tttttggag ggattgggg tttgaatgt cgactacac 
      tactacgtc gaggcggac gtttcgttg gaaaacgtc 
      tgtattgtt gcaaaaaaa taa
    mRNA sequence
    • >TC004062 RA
      aaatgatgc ccccctaag ctttaaatt aggtcaagt 
      gcgacctaa ccttaaacc ttgtgacaa gtcactcag 
      agaggcaaa aaatatgat ttaattaat ccaattacg 
      tacagatta caactgtgc catttctaa gtccccagc 
      cgccacaaa atgagtgag gagcccccc cattgcaac 
      cactttgtg attcgtaaa aagcgcttt tgtcgcatg 
      accgtcaag ccgggtgac acgttttgc ggggagcac 
      caaccggct ggggccaca acggacgat aaaatccga 
      attgtgtgc ccccttgac cctaaacac acgtgttac 
      gcccataat ttgcgcaaa cacttgaaa atttgcaac 
      gcgcggccg ggggccccc gtgccctac ctccagccg 
      ggggtgaat tcaggtggg gcacccgag gaccataaa 
      ctcctgtcg gagtttgcc cccagtgac attgttgca 
      gttattggg aaaattaat acgattttt gaggattgt 
      ttaaggggg aaaattaca agtaagtta ggggggcac 
      aaagtggtg gaggctgaa atggcaaag cccgagtat 
      ggggacaag agcaagaaa cacctaaaa caggcgtcg 
      gcgattttg gggttgttg gacgagagt ggtttgatt 
      aaacccgaa acgtgtttt gtggagttt ggggctggg 
      agggggctg ttgagttat tggttggct caggcgtgc 
      ccccaggcc acttttctc ttaatcgag cggtcttcc 
      cccaaacac aagcgtgat aataaattg gaaaaaagc 
      gataaagtc cgaaggatt cgggcggac attgccgac 
      ttggtttta gataagttg gacgctgtt tcggggaat 
      cccattgtt ggggtcacg aagcatttg tgcggcgac 
      gccactgat ttggccttg agatgtttg gccaacgtt 
      tcaagcgtt aaggtaggg ggtctcact atgactttt 
      tgttgccac cataggtgt cgctggccg agctatgtc 
      ggcaaagat ttttttaat cgatttggt ttaacaaaa 
      aatgatttt gaaatgatg tgtggtatg agcagttgg 
      gccacttgt ggcactggc tttagccgg gagaagaac 
      caaactggg ggcactgtg gagggaaat gacagagac 
      aaggagatt ggagtcacc agaagccag aaggaggag 
      ttagggagg aggtcaaaa gcaatccta aattggggg 
      cgcctccaa tttttggag ggattgggg tttgaatgt 
      cgactacac tactacgtc gaggcggac gtttcgttg 
      gaaaacgtc tgtattgtt gcaaaaaaa taaaaaaat 
      aaatagttg cacgtgtgt ttttttccc tcatttctt 
      ttccttttt ttgtaactt tttttgctt taataaatg 
      tttattatt atttatgtt atttatctt tatttgatt 
      taaaattat tttttatct gtgttagaa taaagtatg 
      caactacta