Status
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Gene information
General information
TC031718
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004059,TC004058
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451702.1: 82801..96543
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031718
    iBeetle screen iB_03680

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031718 RA
      MSILATFAV LLTFSAQIS PKLLNLKPP QENSFRRQF 
      VKDLIDEYK TFQVIFHYD NDSSHHLNQ FLKRWAEKT 
      TPWIIFNHD LNQTSDKPF LVKSLHVVS LQNPTVFTN 
      YLQIKRNVG ASDVVVFLI DIFDLKYFE KNFWFMPRL 
      ETAGNVLVV DLTEEKSIN VFRICFFCG KNSGKLAKI 
      QTIRKTGEK VPPQYLYPR KFNNFEGHT FKIAYLDYY 
      PYAYHQKNS TKIMGIDPK LVKMLSQEL NFKYKFYKF 
      NDNLVEKLD SGEFHFAVG GFSLNSQHY SQVQYSFAI 
      NFEKVVAIY YYDTSFRFA LVTFFLPFP LLAWVGIAS 
      GVVGMAVIA YFVVKKWGF YVRREPLLL EWWGKRLYY 
      SSGYKSRLT SIWMKPPMR KSFTLQELQ NDGYDVIVD 
      DFNSEKFGN YVTAKFSQK FDTCQAIEH IRSHKSVHI 
      NEMSRVFLH SRLFCPKIR FYQKWGTRF GVSQVGQTV 
      LGWPFKIGT PFVKVIDDK LKRITSGGH LEQWRREMS 
      KSDRATMSR FRKKILALI FVEILWCCV GASDNNVMK 
      ILRRQSGQP LLKYNTAKE KAKNATSDD ATGYESFVA 
      YDLQIVIDA LEVLNDTQC SRDAMVVME GIARRELWA 
      LQMFDASSK FPVGLLHGN FYQLGNFDE CIKVRHESP 
      IQGQYCLAS VELIKKQSD GRVARSFEK HKDKSRHIF 
      NSTMFWSVC VPSSCQVDE VRRIITQLF TLATKQPIA 
      VTLRDDRCH VAKTEPITP LEIIYGCIV GVFSVFVIF 
      ATVFHYFRA ESGSVVKDA ILCFSLINT VGKFLTTKT 
      SSLNLECLS GIKFISMLF IIAGHTFLF LVGGPVQNT 
      DFYEQESRS LVNAIFVNS PLLVDTFLL VSGFLMCYL 
      LLLELDKRK GRINVLVLY IARYIRLTP SYLVVIGFY 
      CTFLSRIGD GPLWDSRIK HEQEKCQKS WWTNLLYVN 
      NYVNTDDMC MFQSWYLAA DYHLFIIAP FVIYPLWKF 
      AKVGKTVLG LGVFISVAI PFIVTIVYK LDPTLMIFA 
      PEVYEISEN HYFKTAYIK THMRAGSYF FGLFFGYLL 
      YKLQNQGTK LSRRVIWFG WLLCATCGI ISMYSIVVF 
      YAPEHQYNA FESAIYAAL HRVAWCISI GWIMVACIT 
      KNAPLVNRF LSWKPFMPL SRLTYCAYL CNGFIEVYS 
      IGSIRQGTY MGKLELGTK SLAHCLLTF MMAFVLCIF 
      FESPIHGLE KILLRRVPQ KTSKVKAVS NETVSTEI
    CDS sequence
    • >TC031718 RA
      atgtccatt ttggccact tttgctgtt ctactaaca 
      ttttcagca caaatatcc ccaaaacta ctaaatctc 
      aaaccgcca caagaaaac tccttcaga cgccaattc 
      gtcaaagac ctcatcgac gaatacaaa acgtttcaa 
      gtaattttt cactacgat aacgactcc agtcaccac 
      ttaaaccaa tttctcaaa cgctgggcc gaaaaaacc 
      accccttgg attattttt aaccacgat ttaaaccaa 
      acctccgat aaaccattt ctggtcaaa tcacttcac 
      gtcgtatct ttgcaaaac ccaaccgtg ttcacaaac 
      tacctccaa atcaaacga aacgttggg gcaagtgat 
      gtggtggtt ttcctcatc gatattttc gacttgaag 
      tattttgaa aaaaatttt tggttcatg ccacggctt 
      gaaaccgcc ggaaacgtc cttgttgtg gacttgacc 
      gaagaaaaa tctatcaat gtgttcagg atttgcttt 
      ttctgtggc aaaaacagc ggaaaactt gccaaaatc 
      caaacaatt cgtaaaact ggagagaaa gtaccaccc 
      caatatctc tatccaaga aaattcaac aatttcgaa 
      ggccatact ttcaaaatt gcatatctg gactactac 
      ccgtacgcc taccaccaa aaaaactca acaaaaatc 
      atgggaatt gacccaaaa ctcgtcaaa atgctcagc 
      caggaacta aatttcaaa tacaaattt tacaagttt 
      aatgataac ttggtcgag aaactggat tcgggcgaa 
      tttcatttc gcagttggt ggattttcg ctcaattcg 
      cagcattac agtcaggtc cagtacagt tttgccata 
      aatttcgaa aaagtggtg gcaatttat tactacgat 
      accagtttt aggttcgca cttgtgacg ttttttcta 
      ccgtttccg cttttggcg tgggtgggg atagcttcc 
      ggtgtggtg gggatggct gttattgcg tactttgtt 
      gtgaaaaag tggggtttt tacgtaagg cgtgagcct 
      ttactactc gagtggtgg gggaagcgc ctttactac 
      tcgagtggg tacaagtca cgcctgacc tcgatttgg 
      atgaaacca ccaatgagg aaaagtttc acgcttcag 
      gagctacaa aatgacggt tatgacgtc atagttgac 
      gattttaac agcgaaaaa tttggaaat tatgtgact 
      gcgaaattt tcccaaaaa tttgatact tgtcaagcc 
      atcgaacat atcagaagc cacaaaagc gttcacatc 
      aatgaaatg tcgcgtgtt tttctccac tcgaggtta 
      ttttgcccc aaaatcagg ttttaccaa aaatggggc 
      actagattt ggtgttagt caagttggc caaactgtg 
      ctaggttgg ccttttaaa atcgggact ccctttgtt 
      aaagtaatc gacgataag ttgaaacgc atcacttcg 
      ggaggacat ttggaacaa tggcgacgc gaaatgtcc 
      aagagcgat cgcgccaca atgagtcgt tttcgaaaa 
      aaaattctt gcgctaatt tttgtcgaa attttgtgg 
      tgttgtgtt ggtgccagt gacaacaat gtgatgaag 
      attcttcgg cgtcagagc ggccaaccg ctgctcaaa 
      tacaacact gccaaagaa aaggcaaag aacgccaca 
      tctgatgac gcaaccggt tatgaatcc ttcgtagct 
      tacgacctc caaatcgtc atcgacgcc ctcgaggtc 
      ctcaacgac acccagtgc tcccgcgac gccatggtg 
      gtgatggaa ggcatcgcc agaagggag ctctgggcc 
      ttacaaatg tttgacgca tcatcgaaa tttcccgtg 
      ggcctcctc catggcaac ttctaccaa ttaggaaat 
      ttcgacgaa tgcatcaaa gtgcgccat gaaagccca 
      atccaaggt caatactgt ctagcgtcc gttgaacta 
      atcaaaaaa caatcagac ggacgcgta gcgcgctca 
      ttcgaaaaa cacaaggac aaatcccgc cacattttc 
      aacagcacc atgttctgg agcgtctgc gttccatct 
      tcgtgccaa gtcgacgaa gttcgacgc atcatcacg 
      caactattc accttggca acgaaacaa ccaatcgcg 
      gtcacgttg cgtgacgac aggtgtcac gtggccaaa 
      actgaacca atcacgccg ctggagatt atctatggc 
      tgcattgtt ggggttttt agcgttttt gttattttt 
      gcaactgtt tttcattat tttcgagca gagtcaggc 
      agtgttgtc aaagatgcg attctttgt ttctcgtta 
      attaacaca gttggtaaa tttttgaca acaaaaaca 
      tcaagtctt aatctagag tgcttgagt gggattaaa 
      ttcatctca atgttgttt ataatcgcc ggacatacg 
      tttttattt ttggtgggg gggcctgtc caaaatacg 
      gatttttac gaacaggaa agtcgcagt cttgtgaac 
      gcaattttc gttaatagt ccgttatta gttgacaca 
      tttttactt gtcagtggc ttcttgatg tgttatttg 
      ctgttgttg gagcttgac aagagaaag ggtcgcata 
      aatgtgttg gtactttac atagcgcgt tacataaga 
      ctgacgcca tcttacttg gtcgtgatt ggtttttat 
      tgcactttc ttgtcacgg attggtgat gggccactt 
      tgggactca cgaatcaaa catgagcaa gaaaagtgc 
      caaaaatcc tggtggact aacctatta tatgtcaat 
      aattacgtt aacactgac gatatgtgc atgttccaa 
      tcgtggtac ttggcggcc gattatcac ttgtttatc 
      atagccccc ttcgttatt tacccactt tggaagttt 
      gccaaagtt ggcaaaact gttctcgga ctcggggtt 
      tttatcagt gttgccatc ccgtttatt gtcacaatt 
      gtgtataaa ctggacccg actttgatg atttttgca 
      ccggaagtg tacgaaatt agtgaaaat cactacttt 
      aaaacggcg tacattaag acacatatg agggccggc 
      tcgtacttt ttcggccta tttttcggt tatcttttg 
      tacaaattg caaaaccaa ggaaccaaa ttgtcgcga 
      cgtgtaatt tggttcggt tggttgctc tgtgccact 
      tgcggcatt atatcgatg tattcgatc gtggtgttt 
      tatgcccct gagcatcag tataatgcc ttcgagtcg 
      gcgatttat gccgcgctg catcgcgtc gcctggtgc 
      ataagtata gggtggatc atggtggca tgcatcacg 
      aaaaatgcc cctctagtc aatcggttt ctttcgtgg 
      aagcctttt atgccctta agtcggctt acgtactgt 
      gcttatctt tgtaatggt tttatcgaa gtttacagc 
      atcgggtcg ataaggcag ggaacctac atggggaaa 
      ttggagttg gggactaaa tcgttggca cactgcttg 
      ttgacgttt atgatggcg tttgttttg tgcattttc 
      ttcgaatca cccattcat gggttggag aagattttg 
      ttgaggaga gtgcctcaa aagacctca aaagtcaag 
      gccgtgagt aacgagacc gttagtact gagatatga
    mRNA sequence
    • >TC031718 RA
      ggagcacca acgtgacat aattacgtt tgtagttat 
      tgtctagat aacaagttt agttcgaga aaaattact 
      ggtttgggt taattggcg cagtcgctc aactttgac 
      caaattata atgtccatt ttggccact tttgctgtt 
      ctactaaca ttttcagca caaatatcc ccaaaacta 
      ctaaatctc aaaccgcca caagaaaac tccttcaga 
      cgccaattc gtcaaagac ctcatcgac gaatacaaa 
      acgtttcaa gtaattttt cactacgat aacgactcc 
      agtcaccac ttaaaccaa tttctcaaa cgctgggcc 
      gaaaaaacc accccttgg attattttt aaccacgat 
      ttaaaccaa acctccgat aaaccattt ctggtcaaa 
      tcacttcac gtcgtatct ttgcaaaac ccaaccgtg 
      ttcacaaac tacctccaa atcaaacga aacgttggg 
      gcaagtgat gtggtggtt ttcctcatc gatattttc 
      gacttgaag tattttgaa aaaaatttt tggttcatg 
      ccacggctt gaaaccgcc ggaaacgtc cttgttgtg 
      gacttgacc gaagaaaaa tctatcaat gtgttcagg 
      atttgcttt ttctgtggc aaaaacagc ggaaaactt 
      gccaaaatc caaacaatt cgtaaaact ggagagaaa 
      gtaccaccc caatatctc tatccaaga aaattcaac 
      aatttcgaa ggccatact ttcaaaatt gcatatctg 
      gactactac ccgtacgcc taccaccaa aaaaactca 
      acaaaaatc atgggaatt gacccaaaa ctcgtcaaa 
      atgctcagc caggaacta aatttcaaa tacaaattt 
      tacaagttt aatgataac ttggtcgag aaactggat 
      tcgggcgaa tttcatttc gcagttggt ggattttcg 
      ctcaattcg cagcattac agtcaggtc cagtacagt 
      tttgccata aatttcgaa aaagtggtg gcaatttat 
      tactacgat accagtttt aggttcgca cttgtgacg 
      ttttttcta ccgtttccg cttttggcg tgggtgggg 
      atagcttcc ggtgtggtg gggatggct gttattgcg 
      tactttgtt gtgaaaaag tggggtttt tacgtaagg 
      cgtgagcct ttactactc gagtggtgg gggaagcgc 
      ctttactac tcgagtggg tacaagtca cgcctgacc 
      tcgatttgg atgaaacca ccaatgagg aaaagtttc 
      acgcttcag gagctacaa aatgacggt tatgacgtc 
      atagttgac gattttaac agcgaaaaa tttggaaat 
      tatgtgact gcgaaattt tcccaaaaa tttgatact 
      tgtcaagcc atcgaacat atcagaagc cacaaaagc 
      gttcacatc aatgaaatg tcgcgtgtt tttctccac 
      tcgaggtta ttttgcccc aaaatcagg ttttaccaa 
      aaatggggc actagattt ggtgttagt caagttggc 
      caaactgtg ctaggttgg ccttttaaa atcgggact 
      ccctttgtt aaagtaatc gacgataag ttgaaacgc 
      atcacttcg ggaggacat ttggaacaa tggcgacgc 
      gaaatgtcc aagagcgat cgcgccaca atgagtcgt 
      tttcgaaaa aaaattctt gcgctaatt tttgtcgaa 
      attttgtgg tgttgtgtt ggtgccagt gacaacaat 
      gtgatgaag attcttcgg cgtcagagc ggccaaccg 
      ctgctcaaa tacaacact gccaaagaa aaggcaaag 
      aacgccaca tctgatgac gcaaccggt tatgaatcc 
      ttcgtagct tacgacctc caaatcgtc atcgacgcc 
      ctcgaggtc ctcaacgac acccagtgc tcccgcgac 
      gccatggtg gtgatggaa ggcatcgcc agaagggag 
      ctctgggcc ttacaaatg tttgacgca tcatcgaaa 
      tttcccgtg ggcctcctc catggcaac ttctaccaa 
      ttaggaaat ttcgacgaa tgcatcaaa gtgcgccat 
      gaaagccca atccaaggt caatactgt ctagcgtcc 
      gttgaacta atcaaaaaa caatcagac ggacgcgta 
      gcgcgctca ttcgaaaaa cacaaggac aaatcccgc 
      cacattttc aacagcacc atgttctgg agcgtctgc 
      gttccatct tcgtgccaa gtcgacgaa gttcgacgc 
      atcatcacg caactattc accttggca acgaaacaa 
      ccaatcgcg gtcacgttg cgtgacgac aggtgtcac 
      gtggccaaa actgaacca atcacgccg ctggagatt 
      atctatggc tgcattgtt ggggttttt agcgttttt 
      gttattttt gcaactgtt tttcattat tttcgagca 
      gagtcaggc agtgttgtc aaagatgcg attctttgt 
      ttctcgtta attaacaca gttggtaaa tttttgaca 
      acaaaaaca tcaagtctt aatctagag tgcttgagt 
      gggattaaa ttcatctca atgttgttt ataatcgcc 
      ggacatacg tttttattt ttggtgggg gggcctgtc 
      caaaatacg gatttttac gaacaggaa agtcgcagt 
      cttgtgaac gcaattttc gttaatagt ccgttatta 
      gttgacaca tttttactt gtcagtggc ttcttgatg 
      tgttatttg ctgttgttg gagcttgac aagagaaag 
      ggtcgcata aatgtgttg gtactttac atagcgcgt 
      tacataaga ctgacgcca tcttacttg gtcgtgatt 
      ggtttttat tgcactttc ttgtcacgg attggtgat 
      gggccactt tgggactca cgaatcaaa catgagcaa 
      gaaaagtgc caaaaatcc tggtggact aacctatta 
      tatgtcaat aattacgtt aacactgac gatatgtgc 
      atgttccaa tcgtggtac ttggcggcc gattatcac 
      ttgtttatc atagccccc ttcgttatt tacccactt 
      tggaagttt gccaaagtt ggcaaaact gttctcgga 
      ctcggggtt tttatcagt gttgccatc ccgtttatt 
      gtcacaatt gtgtataaa ctggacccg actttgatg 
      atttttgca ccggaagtg tacgaaatt agtgaaaat 
      cactacttt aaaacggcg tacattaag acacatatg 
      agggccggc tcgtacttt ttcggccta tttttcggt 
      tatcttttg tacaaattg caaaaccaa ggaaccaaa 
      ttgtcgcga cgtgtaatt tggttcggt tggttgctc 
      tgtgccact tgcggcatt atatcgatg tattcgatc 
      gtggtgttt tatgcccct gagcatcag tataatgcc 
      ttcgagtcg gcgatttat gccgcgctg catcgcgtc 
      gcctggtgc ataagtata gggtggatc atggtggca 
      tgcatcacg aaaaatgcc cctctagtc aatcggttt 
      ctttcgtgg aagcctttt atgccctta agtcggctt 
      acgtactgt gcttatctt tgtaatggt tttatcgaa 
      gtttacagc atcgggtcg ataaggcag ggaacctac 
      atggggaaa ttggagttg gggactaaa tcgttggca 
      cactgcttg ttgacgttt atgatggcg tttgttttg 
      tgcattttc ttcgaatca cccattcat gggttggag 
      aagattttg ttgaggaga gtgcctcaa aagacctca 
      aaagtcaag gccgtgagt aacgagacc gttagtact 
      gagatatga ttggtggtt atttatttg tacactctg 
      tatattact gttgactat ttgtaaatt ttgtaaaat 
      aaattttta aaaaattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY