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Gene information
General information
TC033895
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004041
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 16367100..16423243
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033895
    iBeetle screen iB_03678

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033895 RA
      MWRNVAITY PRNCVRYLN SVTIDATEL QHFKRFTSE 
      WWDESGYLK ALHALNKLR VPFIRDGII NSAVMLQEH 
      INISLPIKG LSILEVGCG GGILTEPLA RIGADVTGL 
      DANPDLIER AKLHSKNDN LNIKYICSP VEKYAVVNV 
      EKHDAIVAS EILEHVTKK KEFLKHCVK CMKPGGSIF 
      ITTINKTVV AKLFGVVIA ESFFKLLPE GTHQYDKFI 
      EPHKLERIL EELGCKTIL THGIFYNVL TNEWRWCSD 
      DSINYALHA VKLK
    CDS sequence
    • >TC033895 RA
      atgtggaga aatgtagca attacgtat ccgaggaat 
      tgcgtgcgg tatttaaat agtgttact atagatgct 
      accgaatta cagcatttt aaacggttt acatctgaa 
      tggtgggac gaatcggga tatttaaag gctcttcat 
      gctttgaac aaattacgg gtaccattt attcgagat 
      ggtataata aattctgca gttatgcta caggagcat 
      ataaatatt tcattacct ataaaagga ctttcaatt 
      ctagaagtt ggttgtggt ggtggaata ttaacggaa 
      cctttagct cgaataggc gctgatgta actggcctc 
      gatgccaac ccagattta atcgaaaga gcaaaactt 
      cattcaaaa aatgataat ttaaacata aaatatatt 
      tgttcacct gttgagaaa tatgctgtt gtgaatgtg 
      gaaaagcat gacgccatt gttgcatca gaaatcctt 
      gaacacgtt actaagaaa aaggaattt ttaaaacat 
      tgtgtcaag tgtatgaaa cctggagga tccattttc 
      atcacaacc attaataaa accgtggtt gcaaaactt 
      tttggagtg gttatagcg gaaagtttt ttcaaactt 
      ttgcccgaa ggaacccat caatatgat aagtttata 
      gaacctcat aagttagaa aggatatta gaagaactt 
      ggatgtaaa acgatactt acacatggc attttttat 
      aacgtttta acaaatgag tggcgatgg tgttccgac 
      gattctatt aattatgct ttacatgct gttaaattg 
      aaataa
    mRNA sequence
    • >TC033895 RA
      ctaccgtca cgtcacatg ttagaaaca aaacagacc 
      aattgtaac atttataac gtattcttt attttataa 
      gtttataag tagcacttg aatagtgtc tgtgagttt 
      gctgtgttc ttcatattc aaaaataaa cttgtcaag 
      gtgaagttt gcatccaag taaatcaga tattagtgg 
      tatgagaaa atggattaa ggtaaataa atttataac 
      taataacta attcgatat tcagagaat gcagaccca 
      tctaattca atattcagg gaatgcaga cccactatt 
      taccaaatt aaatcaatc aaacagacg atgaaaacc 
      aacaactag gtaaataat gtatttgtt tttcccatt 
      tagaatctg aagtgttcc ggcaccagc cagttgtag 
      atttcgagc caccctctt ccagtcttt aattgaaga 
      tttctgccg ggtttatca ctgcacgga aaaaaacac 
      atttcgaaa tgtggagaa atgtagcaa ttacgtatc 
      cgaggaatt gcgtgcggt atttaaata gtgttacta 
      tagatgcta ccgaattac agcatttta aacggttta 
      catctgaat ggtgggacg aatcgggat atttaaagg 
      ctcttcatg ctttgaaca aattacggg taccattta 
      ttcgagatg gtataataa attctgcag ttatgctac 
      aggagcata taaatattt cattaccta taaaaggac 
      tttcaattc tagaagttg gttgtggtg gtggaatat 
      taacggaac ctttagctc gaataggcg ctgatgtaa 
      ctggcctcg atgccaacc cagatttaa tcgaaagag 
      caaaacttc attcaaaaa atgataatt taaacataa 
      aatatattt gttcacctg ttgagaaat atgctgttg 
      tgaatgtgg aaaagcatg acgccattg ttgcatcag 
      aaatccttg aacacgtta ctaagaaaa aggaatttt 
      taaaacatt gtgtcaagt gtatgaaac ctggaggat 
      ccattttca tcacaacca ttaataaaa ccgtggttg 
      caaaacttt ttggagtgg ttatagcgg aaagttttt 
      tcaaacttt tgcccgaag gaacccatc aatatgata 
      agtttatag aacctcata agttagaaa ggatattag 
      aagaacttg gatgtaaaa cgatactta cacatggca 
      ttttttata acgttttaa caaatgagt ggcgatggt 
      gttccgacg attctatta attatgctt tacatgctg 
      ttaaattga aataataat tgtgtaatt tttgaaaac 
      aataaattt ggccaacag tc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY