Status
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Gene information
General information
TC032700
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004031,TC004032
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid32: 30586..37493
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032700
    iBeetle screen iB_03676
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032700 RA
      MLKFRQLFH TTKCAVVGM VHVGALPGT PLCNKSVDS 
      LVFKACKEA EMYLKYGLD SILVENMHD VPYIQSKYF 
      TPETVATMT RVCTEIRKI APGTVPCGV QVLACGNLE 
      ALAVAKACN FDFIRAEGF VFGHVADEG YTDANAGLI 
      LRYRRQIQA ENVLILADI KKKHSSHAI TSDVSLVET 
      AQAAQFFQA DGLILTGVA TGSPANVSE LSQVKKFCS 
      LPVLVGSGV TGDNLGDYM GADGVIVGS YFKKGGVWY 
      EDVDEERVR NFMEKRKIF ITMALKLLF LVAICLTRT 
      VLCENSTEN FNETETAPQ DLYVIKTVV YEVGILTDA 
      GNDTNTNDT HEQVDVSFF DPSQNGTID LSNIPLPIQ 
      TNVSGVSVT GLLPAANLA AILVPPSDG LPVLPKKQV 
      TVTQNISTN NPDKGSEII SNLPKLLGL TKKNESETR 
      D
    • >TC032700 RB
      MLKFRQLFH TTKCAVVGM VHVGALPGN TPKITPWGH 
      FFTPQKNTH DTCKKRGTP LCNKSVDSL VFKACKEAE 
      MYLKYGLDS ILVENMHDV PYIQSKYFT PETVATMTR 
      VCTEIRKIA PGTVPCGVQ VLACGNLEA LAVAKACNF 
      DFIRAEGFV FGHVADEGY TDANAGLIL RYRRQIQAE 
      NVLILADIK KKHSSHAIT SDVSLVETA QAAQFFQAD 
      GLILTGVAT GSPANVSEL SQVKKFCSL PVLVGSGVT 
      GDNLGDYMG ADGVIVGSY FKKGGVWYE DVDEERVRN 
      FMEKRKIFI TMALKLLFL VAICLTRTV LCENSTENF 
      NETETAPQD LYVIKTVVY EVGILTDAG NDTNTNDTH 
      EQVDVSFFD PSQNGTIDL SNIPLPIQT NVSGVSVTG 
      LLPAANLAA ILVPPSDGL PVLPKKQVT VTQNISTNN 
      PDKGSEIIS NLPKLLGLT KKNESETRD
    CDS sequence
    • >TC032700 RA
      atgctgaaa tttcgccaa ttattccac acgactaaa 
      tgtgcagtg gtcggaatg gtccacgtg ggggcctta 
      ccagggacc ccactttgc aacaaatcg gtcgattct 
      ttggtgttt aaagcatgc aaagaggcg gaaatgtat 
      cttaaatac ggactcgac agcattttg gtcgaaaat 
      atgcatgat gtgccctat atccagtcc aagtatttt 
      acccccgaa actgtggcg acaatgact agggtttgt 
      acggaaatt cggaaaatt gccccaggg acggttccc 
      tgtggcgtc caggttctg gcttgtgga aacctggag 
      gctttagct gtggctaaa gcttgcaat tttgacttt 
      ataagggct gaagggttc gttttcggt cacgtggcc 
      gatgaaggg tacactgac gccaacgcc ggtttgatt 
      ttgcgctat cgcaggcaa atccaggcc gaaaatgtg 
      ctaattttg gccgatatt aagaaaaaa cacagctct 
      catgcaatc actagtgac gtttcattg gttgagacg 
      gctcaagca gcgcagttt tttcaagcc gatggcttg 
      attttgact ggggttgcg actggaagt ccggcaaat 
      gtgtccgaa ttgagtcaa gttaaaaag ttttgttcg 
      ttgccggtt ttggtgggg tctggggtc actggggat 
      aatttgggg gattatatg ggggctgat ggggttatt 
      gtggggtca tattttaaa aagggtggg gtatggtac 
      gaagatgtg gacgaggaa cgtgtgagg aattttatg 
      gagaagaga aaaattttt atcacaatg gcgctcaaa 
      ttattattt cttgtggcg atttgttta acgcgaacg 
      gttttgtgt gaaaatagc accgaaaat tttaatgaa 
      acagaaacg gcaccacag gacttatat gtcataaaa 
      acagttgtt tatgaagtt gggattctc acagatgct 
      ggaaacgat acaaatacg aacgacaca cacgaacaa 
      gtcgatgtt tcgtttttc gatccgtcc caaaacgga 
      actattgac ctctcaaat attccgctt ccgattcaa 
      acaaatgtg agtggtgtt tccgttact ggccttctt 
      cctgctgca aatttagcc gcaattttg gttccgccc 
      agcgatggt ttgcccgtt ttgcccaaa aaacaggtc 
      actgtgacg caaaatatc agcacgaat aatccggat 
      aaaggatcg gagattatt tcgaatttg cccaaactt 
      ttgggtttg acgaagaaa aatgagtcg gagacgaga 
      gattag
    • >TC032700 RB
      atgctgaaa tttcgccaa ttattccac acgactaaa 
      tgtgcagtg gtcggaatg gtccacgtg ggggcctta 
      ccaggtaac acccctaaa atcacacca tgggggcac 
      tttttcaca ccacaaaaa aacacacat gacacgtgc 
      aaaaaacga gggacccca ctttgcaac aaatcggtc 
      gattctttg gtgtttaaa gcatgcaaa gaggcggaa 
      atgtatctt aaatacgga ctcgacagc attttggtc 
      gaaaatatg catgatgtg ccctatatc cagtccaag 
      tattttacc cccgaaact gtggcgaca atgactagg 
      gtttgtacg gaaattcgg aaaattgcc ccagggacg 
      gttccctgt ggcgtccag gttctggct tgtggaaac 
      ctggaggct ttagctgtg gctaaagct tgcaatttt 
      gactttata agggctgaa gggttcgtt ttcggtcac 
      gtggccgat gaagggtac actgacgcc aacgccggt 
      ttgattttg cgctatcgc aggcaaatc caggccgaa 
      aatgtgcta attttggcc gatattaag aaaaaacac 
      agctctcat gcaatcact agtgacgtt tcattggtt 
      gagacggct caagcagcg cagtttttt caagccgat 
      ggcttgatt ttgactggg gttgcgact ggaagtccg 
      gcaaatgtg tccgaattg agtcaagtt aaaaagttt 
      tgttcgttg ccggttttg gtggggtct ggggtcact 
      ggggataat ttgggggat tatatgggg gctgatggg 
      gttattgtg gggtcatat tttaaaaag ggtggggta 
      tggtacgaa gatgtggac gaggaacgt gtgaggaat 
      tttatggag aagagaaaa atttttatc acaatggcg 
      ctcaaatta ttatttctt gtggcgatt tgtttaacg 
      cgaacggtt ttgtgtgaa aatagcacc gaaaatttt 
      aatgaaaca gaaacggca ccacaggac ttatatgtc 
      ataaaaaca gttgtttat gaagttggg attctcaca 
      gatgctgga aacgataca aatacgaac gacacacac 
      gaacaagtc gatgtttcg tttttcgat ccgtcccaa 
      aacggaact attgacctc tcaaatatt ccgcttccg 
      attcaaaca aatgtgagt ggtgtttcc gttactggc 
      cttcttcct gctgcaaat ttagccgca attttggtt 
      ccgcccagc gatggtttg cccgttttg cccaaaaaa 
      caggtcact gtgacgcaa aatatcagc acgaataat 
      ccggataaa ggatcggag attatttcg aatttgccc 
      aaacttttg ggtttgacg aagaaaaat gagtcggag 
      acgagagat tag
    mRNA sequence
    • >TC032700 RA
      ctcacattt gacaaaaaa tgctgaaat ttcgccaat 
      tattccaca cgactaaat gtgcagtgg tcggaatgg 
      tccacgtgg gggccttac cagggaccc cactttgca 
      acaaatcgg tcgattctt tggtgttta aagcatgca 
      aagaggcgg aaatgtatc ttaaatacg gactcgaca 
      gcattttgg tcgaaaata tgcatgatg tgccctata 
      tccagtcca agtatttta cccccgaaa ctgtggcga 
      caatgacta gggtttgta cggaaattc ggaaaattg 
      ccccaggga cggttccct gtggcgtcc aggttctgg 
      cttgtggaa acctggagg ctttagctg tggctaaag 
      cttgcaatt ttgacttta taagggctg aagggttcg 
      ttttcggtc acgtggccg atgaagggt acactgacg 
      ccaacgccg gtttgattt tgcgctatc gcaggcaaa 
      tccaggccg aaaatgtgc taattttgg ccgatatta 
      agaaaaaac acagctctc atgcaatca ctagtgacg 
      tttcattgg ttgagacgg ctcaagcag cgcagtttt 
      ttcaagccg atggcttga ttttgactg gggttgcga 
      ctggaagtc cggcaaatg tgtccgaat tgagtcaag 
      ttaaaaagt tttgttcgt tgccggttt tggtggggt 
      ctggggtca ctggggata atttggggg attatatgg 
      gggctgatg gggttattg tggggtcat attttaaaa 
      agggtgggg tatggtacg aagatgtgg acgaggaac 
      gtgtgagga attttatgg agaagagaa aaattttta 
      tcacaatgg cgctcaaat tattatttc ttgtggcga 
      tttgtttaa cgcgaacgg ttttgtgtg aaaatagca 
      ccgaaaatt ttaatgaaa cagaaacgg caccacagg 
      acttatatg tcataaaaa cagttgttt atgaagttg 
      ggattctca cagatgctg gaaacgata caaatacga 
      acgacacac acgaacaag tcgatgttt cgtttttcg 
      atccgtccc aaaacggaa ctattgacc tctcaaata 
      ttccgcttc cgattcaaa caaatgtga gtggtgttt 
      ccgttactg gccttcttc ctgctgcaa atttagccg 
      caattttgg ttccgccca gcgatggtt tgcccgttt 
      tgcccaaaa aacaggtca ctgtgacgc aaaatatca 
      gcacgaata atccggata aaggatcgg agattattt 
      cgaatttgc ccaaacttt tgggtttga cgaagaaaa 
      atgagtcgg agacgagag attagagaa ttgtgtgat 
      gtgtgatta tttattaaa gatggaata attaagttg 
      gtgattaat aaagtttaa tgatttta
    • >TC032700 RB
      ctcacattt gacaaaaaa tgctgaaat ttcgccaat 
      tattccaca cgactaaat gtgcagtgg tcggaatgg 
      tccacgtgg gggccttac caggtaaca cccctaaaa 
      tcacaccat gggggcact ttttcacac cacaaaaaa 
      acacacatg acacgtgca aaaaacgag ggaccccac 
      tttgcaaca aatcggtcg attctttgg tgtttaaag 
      catgcaaag aggcggaaa tgtatctta aatacggac 
      tcgacagca ttttggtcg aaaatatgc atgatgtgc 
      cctatatcc agtccaagt attttaccc ccgaaactg 
      tggcgacaa tgactaggg tttgtacgg aaattcgga 
      aaattgccc cagggacgg ttccctgtg gcgtccagg 
      ttctggctt gtggaaacc tggaggctt tagctgtgg 
      ctaaagctt gcaattttg actttataa gggctgaag 
      ggttcgttt tcggtcacg tggccgatg aagggtaca 
      ctgacgcca acgccggtt tgattttgc gctatcgca 
      ggcaaatcc aggccgaaa atgtgctaa ttttggccg 
      atattaaga aaaaacaca gctctcatg caatcacta 
      gtgacgttt cattggttg agacggctc aagcagcgc 
      agttttttc aagccgatg gcttgattt tgactgggg 
      ttgcgactg gaagtccgg caaatgtgt ccgaattga 
      gtcaagtta aaaagtttt gttcgttgc cggttttgg 
      tggggtctg gggtcactg gggataatt tgggggatt 
      atatggggg ctgatgggg ttattgtgg ggtcatatt 
      ttaaaaagg gtggggtat ggtacgaag atgtggacg 
      aggaacgtg tgaggaatt ttatggaga agagaaaaa 
      tttttatca caatggcgc tcaaattat tatttcttg 
      tggcgattt gtttaacgc gaacggttt tgtgtgaaa 
      atagcaccg aaaatttta atgaaacag aaacggcac 
      cacaggact tatatgtca taaaaacag ttgtttatg 
      aagttggga ttctcacag atgctggaa acgatacaa 
      atacgaacg acacacacg aacaagtcg atgtttcgt 
      ttttcgatc cgtcccaaa acggaacta ttgacctct 
      caaatattc cgcttccga ttcaaacaa atgtgagtg 
      gtgtttccg ttactggcc ttcttcctg ctgcaaatt 
      tagccgcaa ttttggttc cgcccagcg atggtttgc 
      ccgttttgc ccaaaaaac aggtcactg tgacgcaaa 
      atatcagca cgaataatc cggataaag gatcggaga 
      ttatttcga atttgccca aacttttgg gtttgacga 
      agaaaaatg agtcggaga cgagagatt agagaattg 
      tgtgatgtg tgattattt attaaagat ggaataatt 
      aagttggtg attaataaa gtttaatga tttta