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Gene information
General information
TC004026 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid31: 608943..611645
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004026
    iBeetle screen iB_03673
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004026 RA
      MLLILTLSL SLVIGTCHS TTYEGHKVY KLYPTTPAH 
      NEFLTSLKS TEFDFWSPI NNVGKPVTV MASPQVQKK 
      FEKKLKKHK ISYELEIDN LQSTIDQEK KYHEDKLGI 
      AFDHYLRHS EINNYLDQL AQNYPNIVS VGTIGTSYE 
      NRTMKTITI SAKPGPKPV IFIEAGMHA REWIAPALS 
      LYIINQLVE NPSYGFLQD VDWVILPVM NPDGYEYTW 
      TTDRMWRKT RSPGSSCNG TDGNRNFGF HWMEKGASG 
      SECSETYAG SEAFSEIET QNLRNVLNK TGNITAFIS 
      LHSYGQYLF YPYSYDYVR ADNWKELAF LAHKVNDVI 
      KSVNGTEYT IGNSAEVLY PAAGCSDDW AMGGAGVRL 
      VYVFELPGG GKYGFDLPP EKILGVCEE TWEGIKVIA 
      DYVVQNCK
    CDS sequence
    • >TC004026 RA
      atgttgctt atcttaaca ctaagtttg tctcttgtg 
      atcggtact tgccatagt accacctac gaaggtcat 
      aaagtgtac aaactttac ccaacaacc ccagcacac 
      aatgaattt ttaaccagt ttgaaatca accgaattt 
      gacttttgg tccccaatt aacaatgtc ggtaaacca 
      gtcactgtt atggcttca ccccaagtc caaaaaaaa 
      ttcgaaaaa aaactcaaa aaacacaaa ataagttat 
      gaacttgaa atcgacaac ttacaaagt accatcgat 
      caggagaaa aagtaccat gaagacaag cttggtatt 
      gcttttgac cattactta cgccactct gaaatcaac 
      aattatttg gaccaactc gcccaaaac tatcccaat 
      atagtgtca gttggtacc attggtact tcgtacgaa 
      aacagaacc atgaaaaca atcacaatt tcggcaaaa 
      cctggccca aaacccgtc atttttatc gaagctgga 
      atgcatgcc agagaatgg attgctcca gctttgtcc 
      ctttacatc atcaaccaa ctggtggaa aatccctct 
      tatgggttc ctccaggac gttgactgg gtcattctc 
      ccggtcatg aacccagat gggtacgaa tacacctgg 
      accacggac agaatgtgg cgcaaaaca cgatcaccg 
      ggtagttcc tgcaatggt accgatggt aaccgcaat 
      tttggcttc cactggatg gaaaaaggt gccagtggt 
      tccgaatgt tcggaaact tatgccggt agtgaggcg 
      ttttccgaa atcgagact caaaacttg agaaatgtt 
      ttaaacaaa actggaaac attactgcc tttatttcc 
      ttgcacagt tatggacag tacttgttc tatccgtac 
      agttatgac tacgtgagg gctgataat tggaaggag 
      ttggcgttt ttggcacac aaggtgaac gatgtgatc 
      aagtcggtc aatggaacg gaatatacc attgggaat 
      tcggcggag gttttgtac ccagctgct gggtgtagt 
      gatgattgg gccatgggt ggcgctggg gtcaggttg 
      gtttatgtt tttgaactg cctggaggt gggaaatat 
      gggtttgat ctgccgccg gagaaaatc ttgggggtg 
      tgtgaggag acatgggag gggattaaa gttatagct 
      gattatgta gtccagaat tgtaaataa
    mRNA sequence
    • >TC004026 RA
      caatcacag tttattaaa atgttgctt atcttaaca 
      ctaagtttg tctcttgtg atcggtact tgccatagt 
      accacctac gaaggtcat aaagtgtac aaactttac 
      ccaacaacc ccagcacac aatgaattt ttaaccagt 
      ttgaaatca accgaattt gacttttgg tccccaatt 
      aacaatgtc ggtaaacca gtcactgtt atggcttca 
      ccccaagtc caaaaaaaa ttcgaaaaa aaactcaaa 
      aaacacaaa ataagttat gaacttgaa atcgacaac 
      ttacaaagt accatcgat caggagaaa aagtaccat 
      gaagacaag cttggtatt gcttttgac cattactta 
      cgccactct gaaatcaac aattatttg gaccaactc 
      gcccaaaac tatcccaat atagtgtca gttggtacc 
      attggtact tcgtacgaa aacagaacc atgaaaaca 
      atcacaatt tcggcaaaa cctggccca aaacccgtc 
      atttttatc gaagctgga atgcatgcc agagaatgg 
      attgctcca gctttgtcc ctttacatc atcaaccaa 
      ctggtggaa aatccctct tatgggttc ctccaggac 
      gttgactgg gtcattctc ccggtcatg aacccagat 
      gggtacgaa tacacctgg accacggac agaatgtgg 
      cgcaaaaca cgatcaccg ggtagttcc tgcaatggt 
      accgatggt aaccgcaat tttggcttc cactggatg 
      gaaaaaggt gccagtggt tccgaatgt tcggaaact 
      tatgccggt agtgaggcg ttttccgaa atcgagact 
      caaaacttg agaaatgtt ttaaacaaa actggaaac 
      attactgcc tttatttcc ttgcacagt tatggacag 
      tacttgttc tatccgtac agttatgac tacgtgagg 
      gctgataat tggaaggag ttggcgttt ttggcacac 
      aaggtgaac gatgtgatc aagtcggtc aatggaacg 
      gaatatacc attgggaat tcggcggag gttttgtac 
      ccagctgct gggtgtagt gatgattgg gccatgggt 
      ggcgctggg gtcaggttg gtttatgtt tttgaactg 
      cctggaggt gggaaatat gggtttgat ctgccgccg 
      gagaaaatc ttgggggtg tgtgaggag acatgggag 
      gggattaaa gttatagct gattatgta gtccagaat 
      tgtaaataa ataaagtta aataaagtt cggtctaaa 
      aactagaat aaataatct acaaaaaa