Status
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Gene information
General information
TC032682
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004011,TC004012
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid30: 970..22257
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032682
    iBeetle screen iB_03671
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032682 RA
      MTSMSSTLR QSIFILIKV GVLILVINT VDCIVVNKN 
      ETLNRLIRA SNGNPVLGN CYPSKSIAQ KNYYLIKNA 
      LMLEPSWTN IDPSSTALP EYEKYYWKY TATRETLEK 
      TSENYDLSK HQAENNQSL KFDRFVTPP GVYKITTTL 
      TPKDEFKNL GQIPSFDCF FEIRALNPL VVIQGGPDR 
      VVPNDTEIV VSGADSIDP NTPLGENVL QFEWACTKL 
      KSKDPCAPK ANSTGKEFR IPAEEAIDE EIFEVQVTV 
      TTQFQTSSH NRQTIEVAK NAALLRISC EKNCPPKTD 
      SPNVQDVTF LRAICEKNC KDIKDDDYA WEINQYWPV 
      YGSFSFDYN TQTHFGRIG LKFIIKKNE LKEGYFYNI 
      ELHLRPKAK RYGSTSITL KYASAPLIE RCDTTPNIG 
      LSLTTKFRF SCVQESPDN TFFYELYYS DEKTGSAGN 
      LIISGLYPD VFNYQFLLP PSIGHQFYV KVSKSGGPV 
      TYKQLYPNV VSVTDRKTG PEVVEIYKK FYEGSEKSI 
      SKMVGSNNA KERSEGAEK FQALVEQII SKKIESNDA 
      KSFIKNLTV EVVRDALRS RAGDVNDLL TISDSITKL 
      INQHEVTLL SPVIAKMTT TICKKMIDK HAEAIETNN 
      QALTSKDQM ELIQTSLTK CLEAAVNED YWALEPLNR 
      SITIKELPA VPLEAYIVE NYPDYVDQD KHYTEQMEN 
      FNTITNNVV QATAKAAKA LAMTMVAGQ HVSRSFGQS 
      SATVVAKDA GKYIPHTKI NLLNVTLIP SKDFHNIDH 
      DFDLLMSVW TKDLMWWNH AKTRPATSI VSVQFWHAS 
      CKKRIRHFL NYVELQLKV TKDFKTQLK PGNVTVPDN 
      YLELEGDQL NKYIRVRRV GFPKHSTLY FDFFNMSDE 
      NALDVVFTQ FEFPTIDHF RKNMTIDRD NMTSMKITN 
      HNSYDVWAY IGVLPNRRV VENGTTSKI LHYNLTLVS 
      SFCIRWVKE DRTWQLDCK IGEESTRHD RIICQCKHF 
      SVISGYFQN LNSIVQEVV TIQVVLVLQ SCYIIFFTV 
      AVTYAIFCC FFFLTMFKT YKQVFYLAD NVIDDLYAY 
      LIIVRTENV HHAGTTSKI TIRLKGDKR ISEPHVLNY 
      PDPKLRLLQ NQSEDIFVL TTNRHLGVI KEIELWFDC 
      SGFNPSWYC RDIVIYDMQ KRIQVYFKV KTRFAVDRK 
      GVNYLCIGP REEKGKVTN HIRICNHFH TWMLWAGET 
      NFSYETRLT VVFSNILMI YMFVLLFLG TPTLTLKDC 
      LDRFEEFIF NFDLLILAM QCAFLSFLV HIPLAWTFR 
      FSQIEFKIF DTVYHKSHR YSNYCRTLL CIFVISSTS 
      FLIIYGFWV PVNSSLVWL VTSLVACLF SIVVIENVF 
      IIIQTSICK FKLRVSFDK KFNAILRNI EKQRLFLYG 
      RFNESLFRP LLQHLYAPL KAGQLLRRR IVVELRKSI 
      YEDLQDLFM YSLYNFILY MFILVYHGT LTTVSKSEI 
      ESMVQGEHT RTLKFSDIE SIPDVYRYI NTTLIQIIH 
      PTIWYTNFT IPPSGIMID NANKVIGVA RIRQHRIAA 
      NACEVPAQM NFLNMSCQP EFSFQDAET GDFLPKWAI 
      SVTGVEYDR LKKIWKYAT QSETETWGT VGWIGTYSG 
      GGYVAFLGR TLRNSYANF KKIFDMSWI DHQTRSIFI 
      EFLTYNANY NVFVAVNLL FEQSATGYT SKILQINVV 
      RMLYTQNEV EIESMVYFV LFLFWVSML NIKLLFGIA 
      KNLTDFYKK FWLMLDVVI NFMSLLSIS MFILRIKMV 
      QRYLTSLEA VKHNDFISY FNMMYLEEF LTYFVAVLV 
      CFATARLWK FLRFGQFFR IMERTLAMA AVPLGAVTF 
      AFGVIITAF AFVGIIIFN NEFVDLSFF VKIVATLIQ 
      LSLKPDQFD LMKFITFKL AYVYFSLYL IIMQVILLV 
      YITVIIMAY VKARLELSC VPDRYTVKD YVKEHSEYI 
      PIFVKSLIA RLRAGGSEE PPLTPKADE FRYANCVGV 
      AKNRLKSMR FIVSCIFRN MKEKEEDQR VLTDHETTL 
      MLGVCNTFV LKSSEDDEQ ELFFKGHFE GQNIQLVDE 
      KRIDKIAAF VDIMFEDRP EFGHIAATD SEKTLIDCC 
      KLAKRQNET LRKCTLLLK LVEFKMGDV DRLVNGFVE 
      DVRRSS
    CDS sequence
    • >TC032682 RA
      atgacatca atgagcagt actttaagg caaagtatt 
      tttatttta attaaagtc ggagtttta atactagtg 
      ataaataca gtagactgt attgttgtg aataagaac 
      gaaacgtta aataggcta attagagca agtaacgga 
      aacccagtt ctcggcaat tgttatccg agcaaatcc 
      atcgcgcaa aaaaactac tacttaatt aaaaatgcg 
      cttatgctt gagccaagc tggacaaac atagaccca 
      agttcaacg gctttgcca gagtatgaa aaatactat 
      tggaaatac actgcaaca agagagaca ctcgaaaaa 
      accagcgaa aactacgac ttatccaaa caccaagcc 
      gaaaacaac caaagcttg aaattcgac cgttttgta 
      acccctcca ggtgtctac aaaatcaca accactcta 
      accccaaaa gacgaattc aaaaatttg gggcaaatt 
      ccctctttt gactgcttt ttcgaaatc agagctttg 
      aacccgctt gttgtgatc caaggtggt ccagatcgc 
      gtcgttccc aatgatact gaaattgtg gtaagtggg 
      gccgattcc atcgaccct aacacacca cttggtgaa 
      aatgtgctg caatttgaa tgggcttgc acgaagttg 
      aaaagcaaa gatccatgt gctcccaaa gcaaattcc 
      actgggaaa gagtttcgc attccggca gaggaagcc 
      attgacgaa gagattttt gaagttcaa gttaccgtc 
      accacacag tttcaaact tcgagtcat aaccggcaa 
      acaattgag gttgcgaaa aatgcggca ctgctgagg 
      atcagctgc gagaaaaac tgtcctcct aaaaccgac 
      tcgcccaac gttcaagac gtgacattt ttgcgcgca 
      atttgcgaa aaaaattgc aaagatata aaagacgat 
      gactacgcc tgggaaatc aatcaatac tggcccgta 
      tatggttcc ttcagtttc gattataac acacagaca 
      catttcggc cgaatcggc cttaaattc atcattaag 
      aagaatgag cttaaagaa ggctacttt tacaatata 
      gaactgcat cttcgaccg aaagctaaa cgttacgga 
      agcaccagt attacgctg aaatacgct tcagcaccg 
      cttatagaa cgttgtgat acaactcca aatatcggt 
      ctcagtctc acgaccaaa tttcgcttt agttgcgta 
      caagaatca cccgataat acatttttc tacgagttg 
      tactacagt gatgagaaa actggatca gctggaaat 
      ttaataatc agcggcttg tatccggac gttttcaat 
      tatcaattt ttattgcca ccgtcaatc ggccaccaa 
      ttttacgtt aaagtgtcg aaaagtggc ggacctgtt 
      acttataaa caactctat ccgaatgtg gtttcggtc 
      actgatcgt aaaacaggc cctgaagtt gttgaaatc 
      tacaaaaag ttttacgaa ggcagcgaa aaatcgatt 
      tcgaaaatg gttggtagt aataatgcc aaagaaaga 
      tcggaaggt gcggagaag tttcaagca cttgttgaa 
      caaattatt tcgaagaaa attgagtca aatgatgct 
      aaaagtttt attaagaat ttgacggtg gaagttgtt 
      cgtgatgcg ttaagatca agagcagga gatgttaat 
      gatttattg accataagt gattccatt acaaaatta 
      atcaaccag catgaggtg acacttttg agtccggtg 
      attgctaaa atgacaaca acgatttgt aagaaaatg 
      attgataaa catgcggaa gcaattgag acgaataat 
      caagcttta acgtccaag gatcaaatg gaacttatc 
      caaacttct ttgacgaaa tgtttggaa gctgctgta 
      aatgaagat tactgggct ttggagcct cttaatcgg 
      tctataact ataaaggag ttgccagca gtgcctctg 
      gaagcttac attgtggaa aattatccg gattatgtc 
      gatcaagat aaacattac acggaacaa atggaaaat 
      ttcaatacg atcacaaat aacgttgtt caagccaca 
      gcaaaagcg gccaaagca ttagccatg accatggtg 
      gcaggtcaa cacgtttcc cgaagcttt ggccaaagc 
      tccgccact gttgtagcc aaagacgcc ggaaaatac 
      atccctcac accaaaatc aatttactg aacgtgacc 
      ttaatccca tctaaagat ttccataac attgaccac 
      gatttcgat cttttgatg tccgtctgg accaaagat 
      ttgatgtgg tggaaccat gcaaaaaca cgtccagct 
      actagtatc gtaagtgtg caattttgg cacgcttct 
      tgcaagaaa cgaatacgg catttttta aactacgtg 
      gagcttcaa cttaaagta acgaaagat tttaagacg 
      cagttgaag ccaggaaat gttaccgtt cctgacaat 
      tatctcgaa ttggaagga gatcaattg aataagtac 
      ataagggtt cgaagggtt ggttttccc aaacactcg 
      actctttat ttcgatttt ttcaacatg agtgatgaa 
      aatgctctt gatgtggtt tttacccaa ttcgaattt 
      ccaacaatc gatcatttt cgcaagaac atgaccatc 
      gatcgtgac aacatgacg tcgatgaaa atcaccaat 
      cataacagt tacgacgtt tgggcatac attggtgtt 
      ttgccaaat cgaagagtt gttgaaaat ggaacaaca 
      tcaaaaatt ttgcactac aatctaact ctggtttct 
      tcgttttgt attcgttgg gtgaaagaa gatcgcacc 
      tggcaactt gattgtaaa attggtgaa gagtcaaca 
      cgtcatgat cgtattatt tgtcagtgc aaacatttt 
      tcggtgatt agtggttat tttcaaaat ttgaactca 
      atcgttcaa gaagtggtg acgattcaa gtggttttg 
      gtgctacaa tcgtgctac attattttt ttcactgtt 
      gcagtgact tatgccatt ttttgttgt ttcttcttc 
      ttgacaatg tttaagact tataagcag gtgttttat 
      cttgctgat aacgttata gatgattta tatgcctat 
      ttgataatc gttagaacg gaaaacgtc catcatgct 
      ggtactact tccaaaatc accatcaga cttaaaggg 
      gataaaagg ataagtgag ccacatgtc ctgaactat 
      cctgatcct aaactgcgc cttttgcaa aaccaaagc 
      gaagacatt tttgtgtta acaacaaat cgtcatctt 
      ggtgttatc aaagaaatc gagttgtgg tttgattgt 
      agcggtttc aacccttct tggtattgt cgtgatatc 
      gtcatctac gacatgcaa aaacgtatc caagtttat 
      ttcaaagtg aagacacgt ttcgctgtt gatcgaaaa 
      ggtgttaac tacctttgt atcggccca agagaagaa 
      aaaggcaaa gttacaaac cacataaga atctgtaat 
      cacttccat acgtggatg ttatgggct ggagagacg 
      aattttagc tacgaaacc agactaact gttgttttc 
      tccaacatt ctgatgatt tatatgttt gtgttgctg 
      ttcctcggt actccaaca ctaaccctt aaggactgt 
      cttgacaga ttcgaagaa tttattttt aattttgat 
      ttgttgatt ctggcaatg cagtgtgcg tttttgtcg 
      tttctcgtt cacatacct cttgcttgg acgttcaga 
      ttttcacaa attgaattt aaaatcttc gatacggtt 
      tatcacaaa tcgcacaga tactcgaat tattgcagg 
      acgttgttg tgtattttt gtcatatct tcgacttca 
      tttttgatt atttatgga ttttgggtg cctgttaat 
      tccagtttg gtttggttg gtgacgagt ttagtggct 
      tgtcttttt tcaattgtg gtgattgag aatgttttt 
      attataata cagacgtcc atctgtaaa tttaaactg 
      agagtttcg tttgataaa aaatttaac gcaattctt 
      cgcaacatt gaaaaacag agattgttt ctgtatggt 
      cggtttaac gagtcgctt tttcgaccc ctattacag 
      catttgtac gcacctttg aaagccgga caattgctt 
      agacgacga attgttgtc gaacttcgc aaaagtatc 
      tacgaagat ttgcaagac ctgttcatg tactcgctt 
      tataacttt atcctttac atgtttatt ctagtctat 
      cacggaaca ctaacaaca gttagtaag agcgaaatc 
      gagagcatg gtccaaggg gaacacacc cgaacttta 
      aaattttcc gatatcgaa tcaattcct gatgtttat 
      cgttacatt aacaccact ttaattcaa atcatccac 
      ccaactatt tggtacaca aatttcacg attccaccc 
      agtggaata atgatagat aatgccaat aaagtgatt 
      ggggttgca aggattcgt caacatcgt attgcagca 
      aatgcgtgt gaagttcca gctcaaatg aattttttg 
      aatatgtcg tgtcagccg gaattttca tttcaagat 
      gcggaaaca ggcgatttt ctgcccaag tgggctatt 
      tccgtaacg ggggtggag tatgacagg ttgaaaaaa 
      atttggaag tatgccact caatcagaa accgaaact 
      tgggggact gtaggctgg atagggacg tacagtggt 
      ggcggttat gtggcattt cttggccgc actcttcgc 
      aactcttat gcaaatttt aaaaaaatc ttcgatatg 
      agttggatc gaccaccag accagaagc atctttatc 
      gaattttta acctacaac gcaaactat aacgttttt 
      gttgccgtt aatttactg tttgaacaa agcgcaaca 
      ggatacact tcgaaaatt cttcaaatc aacgttgtt 
      cgaatgctg tacacccaa aacgaagtc gaaatcgaa 
      tcaatggtc tatttcgtg cttttcctc ttctgggtc 
      agcatgctc aacattaaa ttgctgttc ggaatagcg 
      aaaaatctc accgatttc tacaaaaag ttctggctg 
      atgcttgat gtggtcatc aatttcatg agtcttttg 
      agcatttcg atgtttatt cttcggatt aagatggtc 
      cagcggtat ctcacgtcg ttggaagcg gtgaagcat 
      aacgatttc atcagctat ttcaacatg atgtatttg 
      gaggagttt ttgacttat tttgtggcg gttttggtg 
      tgttttgcc accgccagg ttgtggaag tttcttcgg 
      ttcgggcag tttttccgc attatggag cgcactctt 
      gccatggct gctgtgcct cttggagca gtgacgttc 
      gcctttggg gtgataatc acagctttt gcgtttgtg 
      gggattata attttcaac aacgagttt gttgacttg 
      tcgtttttt gtgaaaatt gtggccacg cttattcag 
      ctttcgctg aaaccggac cagttcgat ttgatgaag 
      ttcataact ttcaagctg gcgtatgtt tacttttct 
      ttgtatttg attataatg caagtgatt cttctggtt 
      tacatcact gttattata atggcttat gtgaaggct 
      cgcttggag ttgtcctgt gttccagac aggtacact 
      gtcaaggac tacgtcaag gaacactcg gaatacatc 
      ccgattttt gtcaaaagt ttaatcgct cgtttacga 
      gctgggggt tccgaagaa ccgcctctg acccccaaa 
      gctgacgaa ttccgctac gcgaattgt gtgggagtt 
      gccaaaaat cggctcaaa tctatgagg tttatcgtt 
      tcgtgtatt tttcgcaac atgaaggaa aaggaagag 
      gatcaaagg gttttgacc gatcatgaa actactttg 
      atgctagga gtttgcaat acatttgtc ttgaaaagc 
      agtgaggat gacgaacag gaactcttc ttcaagggg 
      catttcgaa ggccagaac atacagcta gtggatgag 
      aaaaggatt gataagatt gctgctttt gtggatatc 
      atgtttgag gacaggcct gagtttgga catattgca 
      gcaactgac tcggaaaaa acactcatc gattgttgt 
      aaactggcc aaaaggcaa aatgagact ttgcgcaag 
      tgtaccttg ctgttgaag ttggtggag tttaagatg 
      ggcgacgtt gataggctt gtgaatggg tttgttgaa 
      gatgttagg cgttcttct tga
    mRNA sequence
    • >TC032682 RA
      atgtcataa tgacatcaa tgagcagta ctttaaggc 
      aaagtattt ttattttaa ttaaagtcg gagttttaa 
      tactagtga taaatacag tagactgta ttgttgtga 
      ataagaacg aaacgttaa ataggctaa ttagagcaa 
      gtaacggaa acccagttc tcggcaatt gttatccga 
      gcaaatcca tcgcgcaaa aaaactact acttaatta 
      aaaatgcgc ttatgcttg agccaagct ggacaaaca 
      tagacccaa gttcaacgg ctttgccag agtatgaaa 
      aatactatt ggaaataca ctgcaacaa gagagacac 
      tcgaaaaaa ccagcgaaa actacgact tatccaaac 
      accaagccg aaaacaacc aaagcttga aattcgacc 
      gttttgtaa cccctccag gtgtctaca aaatcacaa 
      ccactctaa ccccaaaag acgaattca aaaatttgg 
      ggcaaattc cctcttttg actgctttt tcgaaatca 
      gagctttga acccgcttg ttgtgatcc aaggtggtc 
      cagatcgcg tcgttccca atgatactg aaattgtgg 
      taagtgggg ccgattcca tcgacccta acacaccac 
      ttggtgaaa atgtgctgc aatttgaat gggcttgca 
      cgaagttga aaagcaaag atccatgtg ctcccaaag 
      caaattcca ctgggaaag agtttcgca ttccggcag 
      aggaagcca ttgacgaag agatttttg aagttcaag 
      ttaccgtca ccacacagt ttcaaactt cgagtcata 
      accggcaaa caattgagg ttgcgaaaa atgcggcac 
      tgctgagga tcagctgcg agaaaaact gtcctccta 
      aaaccgact cgcccaacg ttcaagacg tgacatttt 
      tgcgcgcaa tttgcgaaa aaaattgca aagatataa 
      aagacgatg actacgcct gggaaatca atcaatact 
      ggcccgtat atggttcct tcagtttcg attataaca 
      cacagacac atttcggcc gaatcggcc ttaaattca 
      tcattaaga agaatgagc ttaaagaag gctactttt 
      acaatatag aactgcatc ttcgaccga aagctaaac 
      gttacggaa gcaccagta ttacgctga aatacgctt 
      cagcaccgc ttatagaac gttgtgata caactccaa 
      atatcggtc tcagtctca cgaccaaat ttcgcttta 
      gttgcgtac aagaatcac ccgataata catttttct 
      acgagttgt actacagtg atgagaaaa ctggatcag 
      ctggaaatt taataatca gcggcttgt atccggacg 
      ttttcaatt atcaatttt tattgccac cgtcaatcg 
      gccaccaat tttacgtta aagtgtcga aaagtggcg 
      gacctgtta cttataaac aactctatc cgaatgtgg 
      tttcggtca ctgatcgta aaacaggcc ctgaagttg 
      ttgaaatct acaaaaagt tttacgaag gcagcgaaa 
      aatcgattt cgaaaatgg ttggtagta ataatgcca 
      aagaaagat cggaaggtg cggagaagt ttcaagcac 
      ttgttgaac aaattattt cgaagaaaa ttgagtcaa 
      atgatgcta aaagtttta ttaagaatt tgacggtgg 
      aagttgttc gtgatgcgt taagatcaa gagcaggag 
      atgttaatg atttattga ccataagtg attccatta 
      caaaattaa tcaaccagc atgaggtga cacttttga 
      gtccggtga ttgctaaaa tgacaacaa cgatttgta 
      agaaaatga ttgataaac atgcggaag caattgaga 
      cgaataatc aagctttaa cgtccaagg atcaaatgg 
      aacttatcc aaacttctt tgacgaaat gtttggaag 
      ctgctgtaa atgaagatt actgggctt tggagcctc 
      ttaatcggt ctataacta taaaggagt tgccagcag 
      tgcctctgg aagcttaca ttgtggaaa attatccgg 
      attatgtcg atcaagata aacattaca cggaacaaa 
      tggaaaatt tcaatacga tcacaaata acgttgttc 
      aagccacag caaaagcgg ccaaagcat tagccatga 
      ccatggtgg caggtcaac acgtttccc gaagctttg 
      gccaaagct ccgccactg ttgtagcca aagacgccg 
      gaaaataca tccctcaca ccaaaatca atttactga 
      acgtgacct taatcccat ctaaagatt tccataaca 
      ttgaccacg atttcgatc ttttgatgt ccgtctgga 
      ccaaagatt tgatgtggt ggaaccatg caaaaacac 
      gtccagcta ctagtatcg taagtgtgc aattttggc 
      acgcttctt gcaagaaac gaatacggc attttttaa 
      actacgtgg agcttcaac ttaaagtaa cgaaagatt 
      ttaagacgc agttgaagc caggaaatg ttaccgttc 
      ctgacaatt atctcgaat tggaaggag atcaattga 
      ataagtaca taagggttc gaagggttg gttttccca 
      aacactcga ctctttatt tcgattttt tcaacatga 
      gtgatgaaa atgctcttg atgtggttt ttacccaat 
      tcgaatttc caacaatcg atcattttc gcaagaaca 
      tgaccatcg atcgtgaca acatgacgt cgatgaaaa 
      tcaccaatc ataacagtt acgacgttt gggcataca 
      ttggtgttt tgccaaatc gaagagttg ttgaaaatg 
      gaacaacat caaaaattt tgcactaca atctaactc 
      tggtttctt cgttttgta ttcgttggg tgaaagaag 
      atcgcacct ggcaacttg attgtaaaa ttggtgaag 
      agtcaacac gtcatgatc gtattattt gtcagtgca 
      aacattttt cggtgatta gtggttatt ttcaaaatt 
      tgaactcaa tcgttcaag aagtggtga cgattcaag 
      tggttttgg tgctacaat cgtgctaca ttatttttt 
      tcactgttg cagtgactt atgccattt tttgttgtt 
      tcttcttct tgacaatgt ttaagactt ataagcagg 
      tgttttatc ttgctgata acgttatag atgatttat 
      atgcctatt tgataatcg ttagaacgg aaaacgtcc 
      atcatgctg gtactactt ccaaaatca ccatcagac 
      ttaaagggg ataaaagga taagtgagc cacatgtcc 
      tgaactatc ctgatccta aactgcgcc ttttgcaaa 
      accaaagcg aagacattt ttgtgttaa caacaaatc 
      gtcatcttg gtgttatca aagaaatcg agttgtggt 
      ttgattgta gcggtttca acccttctt ggtattgtc 
      gtgatatcg tcatctacg acatgcaaa aacgtatcc 
      aagtttatt tcaaagtga agacacgtt tcgctgttg 
      atcgaaaag gtgttaact acctttgta tcggcccaa 
      gagaagaaa aaggcaaag ttacaaacc acataagaa 
      tctgtaatc acttccata cgtggatgt tatgggctg 
      gagagacga attttagct acgaaacca gactaactg 
      ttgttttct ccaacattc tgatgattt atatgtttg 
      tgttgctgt tcctcggta ctccaacac taaccctta 
      aggactgtc ttgacagat tcgaagaat ttattttta 
      attttgatt tgttgattc tggcaatgc agtgtgcgt 
      ttttgtcgt ttctcgttc acatacctc ttgcttgga 
      cgttcagat tttcacaaa ttgaattta aaatcttcg 
      atacggttt atcacaaat cgcacagat actcgaatt 
      attgcagga cgttgttgt gtatttttg tcatatctt 
      cgacttcat ttttgatta tttatggat tttgggtgc 
      ctgttaatt ccagtttgg tttggttgg tgacgagtt 
      tagtggctt gtctttttt caattgtgg tgattgaga 
      atgttttta ttataatac agacgtcca tctgtaaat 
      ttaaactga gagtttcgt ttgataaaa aatttaacg 
      caattcttc gcaacattg aaaaacaga gattgtttc 
      tgtatggtc ggtttaacg agtcgcttt ttcgacccc 
      tattacagc atttgtacg cacctttga aagccggac 
      aattgctta gacgacgaa ttgttgtcg aacttcgca 
      aaagtatct acgaagatt tgcaagacc tgttcatgt 
      actcgcttt ataacttta tcctttaca tgtttattc 
      tagtctatc acggaacac taacaacag ttagtaaga 
      gcgaaatcg agagcatgg tccaagggg aacacaccc 
      gaactttaa aattttccg atatcgaat caattcctg 
      atgtttatc gttacatta acaccactt taattcaaa 
      tcatccacc caactattt ggtacacaa atttcacga 
      ttccaccca gtggaataa tgatagata atgccaata 
      aagtgattg gggttgcaa ggattcgtc aacatcgta 
      ttgcagcaa atgcgtgtg aagttccag ctcaaatga 
      attttttga atatgtcgt gtcagccgg aattttcat 
      ttcaagatg cggaaacag gcgattttc tgcccaagt 
      gggctattt ccgtaacgg gggtggagt atgacaggt 
      tgaaaaaaa tttggaagt atgccactc aatcagaaa 
      ccgaaactt gggggactg taggctgga tagggacgt 
      acagtggtg gcggttatg tggcatttc ttggccgca 
      ctcttcgca actcttatg caaatttta aaaaaatct 
      tcgatatga gttggatcg accaccaga ccagaagca 
      tctttatcg aatttttaa cctacaacg caaactata 
      acgtttttg ttgccgtta atttactgt ttgaacaaa 
      gcgcaacag gatacactt cgaaaattc ttcaaatca 
      acgttgttc gaatgctgt acacccaaa acgaagtcg 
      aaatcgaat caatggtct atttcgtgc ttttcctct 
      tctgggtca gcatgctca acattaaat tgctgttcg 
      gaatagcga aaaatctca ccgatttct acaaaaagt 
      tctggctga tgcttgatg tggtcatca atttcatga 
      gtcttttga gcatttcga tgtttattc ttcggatta 
      agatggtcc agcggtatc tcacgtcgt tggaagcgg 
      tgaagcata acgatttca tcagctatt tcaacatga 
      tgtatttgg aggagtttt tgacttatt ttgtggcgg 
      ttttggtgt gttttgcca ccgccaggt tgtggaagt 
      ttcttcggt tcgggcagt ttttccgca ttatggagc 
      gcactcttg ccatggctg ctgtgcctc ttggagcag 
      tgacgttcg cctttgggg tgataatca cagcttttg 
      cgtttgtgg ggattataa ttttcaaca acgagtttg 
      ttgacttgt cgttttttg tgaaaattg tggccacgc 
      ttattcagc tttcgctga aaccggacc agttcgatt 
      tgatgaagt tcataactt tcaagctgg cgtatgttt 
      acttttctt tgtatttga ttataatgc aagtgattc 
      ttctggttt acatcactg ttattataa tggcttatg 
      tgaaggctc gcttggagt tgtcctgtg ttccagaca 
      ggtacactg tcaaggact acgtcaagg aacactcgg 
      aatacatcc cgatttttg tcaaaagtt taatcgctc 
      gtttacgag ctgggggtt ccgaagaac cgcctctga 
      cccccaaag ctgacgaat tccgctacg cgaattgtg 
      tgggagttg ccaaaaatc ggctcaaat ctatgaggt 
      ttatcgttt cgtgtattt ttcgcaaca tgaaggaaa 
      aggaagagg atcaaaggg ttttgaccg atcatgaaa 
      ctactttga tgctaggag tttgcaata catttgtct 
      tgaaaagca gtgaggatg acgaacagg aactcttct 
      tcaaggggc atttcgaag gccagaaca tacagctag 
      tggatgaga aaaggattg ataagattg ctgcttttg 
      tggatatca tgtttgagg acaggcctg agtttggac 
      atattgcag caactgact cggaaaaaa cactcatcg 
      attgttgta aactggcca aaaggcaaa atgagactt 
      tgcgcaagt gtaccttgc tgttgaagt tggtggagt 
      ttaagatgg gcgacgttg ataggcttg tgaatgggt 
      ttgttgaag atgttaggc gttcttctt gaattatag 
      ttttggttc aattattta tgaaataaa agggttttc 
      gttgt