General information | |||
TC003998 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 28917148..28921285 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC003998 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03667 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (16 dpi)
- >TC003998 RA
MELDYQMAL ILQHRFEQE AGQTEQSDS ELAKQLQEQ FEQEAAVEQ PPSSLVYHQ PKKGSDSTK SLIDPSWEL VDPTPDIHN LFIAFDQRF FWNKLVAVC VSWSKKMTS CAGICSYSG RGGLCSITL SEPLLKLRP RKDLVETLL HEMIHAYLF VTHNNRDRD GHGPEFHKH MYRINNEAG TSITVYHNF HDEVRLYQQ HWWRCDGPC QHRKPFFGM VRRATNRAP GPNDRWWAE HLRTCGGTF IKVKEPEKK KQEKKNETK SVKSKDIRD FMGGNVKGF KDLKNGVVR GKSNSSSTI VVTKNTKTS ETTFKPTIF NASTSTILK TETNSFRAS TSTKPSDDY VAVRNHWAN KFNNANKRQ SSEESSTVS KIPKLFGSS PTSTQKNPN SGEFCECPV CNQRVLMAD LNQHLDQCL LKSPEERDA ESVDLTDSF HEEEDTKEC PLCTKEIVT SKFDTHVEK CLMEVYNGV EEKLAVPKA QEETVSCLA CGKKIVKSE LNSHLDECM VDIFDDEES KDKEVCDKN NSQFNCPFC LKLVEESEM KEHIDGCLM ADNLAEAFA DDDF
- >TC003998 RA
atggagctg gactaccaa atggcgctc attttgcag cacaggttt gaacaagaa gctgggcaa accgaacag tctgattct gagttggcc aaacaattg caagaacag ttcgaacag gaggctgca gttgaacag cccccttct agtcttgtc tatcaccag cctaaaaag gggtccgac tcgaccaaa tcactcata gacccctca tgggagctc gtggacccc acccccgac atacacaac ctgttcata gcttttgac caacgtttt ttttggaat aaattggtg gcggtttgt gtctcttgg agcaagaaa atgacttcg tgtgctggc atttgctcg tatagtggc agagggggg ctttgtagt atcaccttg agtgaaccg ttacttaaa ttaaggccc aggaaagac ttagttgag actcttctt catgaaatg attcacgct tatttattt gtaacccat aataataga gacagggat gggcacggg cccgagttt cataaacac atgtaccgg atcaataac gaggcaggg actagtatt accgtttac cataacttc catgatgaa gttcgttta tatcagcaa cactggtgg cgctgtgac ggaccttgt cagcaccgg aagcccttt tttggcatg gtcagaagg gccacaaat agggctccg gggcccaat gataggtgg tgggcagag catttaagg acttgcggt ggaaccttt attaaagtc aaagaaccg gaaaagaag aaacaggag aagaagaat gaaacgaaa agtgtgaag agtaaagat attagagat tttatgggt ggtaatgtt aaaggtttc aaagacttg aaaaatggc gttgttagg ggaaaaagc aactcatca agcactata gtagtcact aaaaacact aaaactagt gaaactact ttcaaaccg acgattttt aatgccagt actagtact attttgaag actgaaaca aatagcttt cgtgctagt actagtact aaaccgagt gatgattat gtagctgtt cgaaatcat tgggccaac aaattcaat aatgccaat aaaagacaa tcatccgaa gaatcatca actgtttcc aaaattccg aaattgttt gggtcttcg cctactagc acccaaaaa aatcccaat tctggtgaa ttttgtgaa tgtcctgtt tgtaatcaa agggtgctg atggctgat ttgaaccaa catctcgat cagtgtttg cttaaatca ccggaagaa agagatgca gaaagtgtt gatcttact gattctttc catgaggag gaagataca aaagaatgt ccactttgt accaaagaa attgtaact tccaaattt gacactcat gttgaaaag tgcctaatg gaggtttat aacggagtt gaggaaaag ttggctgtt ccaaaagcc caagaagaa acggtttcg tgtttggct tgtggtaaa aaaatcgta aaaagtgaa cttaattcg catttggat gagtgtatg gttgatatt ttcgatgat gaggaaagt aaagataag gaagtttgt gataagaat aattcgcag tttaattgt ccattttgt ttgaaactg gtggaagag agcgaaatg aaggaacat attgatggg tgtttgatg gctgataat ctcgccgag gcttttgca gatgatgat ttttga
- >TC003998 RA
attttacag ctaaaacgc ctcaagaca acgatcagg ggccgtttt tgaggttag gatggagct ggactacca aatggcgct cattttgca gcacaggtt tgaacaaga agctgggca aaccgaaca gtctgattc tgagttggc caaacaatt gcaagaaca gttcgaaca ggaggctgc agttgaaca gcccccttc tagtcttgt ctatcacca gcctaaaaa ggggtccga ctcgaccaa atcactcat agacccctc atgggagct cgtggaccc cacccccga catacacaa cctgttcat agcttttga ccaacgttt tttttggaa taaattggt ggcggtttg tgtctcttg gagcaagaa aatgacttc gtgtgctgg catttgctc gtatagtgg cagaggggg gctttgtag tatcacctt gagtgaacc gttacttaa attaaggcc caggaaaga cttagttga gactcttct tcatgaaat gattcacgc ttatttatt tgtaaccca taataatag agacaggga tgggcacgg gcccgagtt tcataaaca catgtaccg gatcaataa cgaggcagg gactagtat taccgttta ccataactt ccatgatga agttcgttt atatcagca acactggtg gcgctgtga cggaccttg tcagcaccg gaagccctt ttttggcat ggtcagaag ggccacaaa tagggctcc ggggcccaa tgataggtg gtgggcaga gcatttaag gacttgcgg tggaacctt tattaaagt caaagaacc ggaaaagaa gaaacagga gaagaagaa tgaaacgaa aagtgtgaa gagtaaaga tattagaga ttttatggg tggtaatgt taaaggttt caaagactt gaaaaatgg cgttgttag gggaaaaag caactcatc aagcactat agtagtcac taaaaacac taaaactag tgaaactac tttcaaacc gacgatttt taatgccag tactagtac tattttgaa gactgaaac aaatagctt tcgtgctag tactagtac taaaccgag tgatgatta tgtagctgt tcgaaatca ttgggccaa caaattcaa taatgccaa taaaagaca atcatccga agaatcatc aactgtttc caaaattcc gaaattgtt tgggtcttc gcctactag cacccaaaa aaatcccaa ttctggtga attttgtga atgtcctgt ttgtaatca aagggtgct gatggctga tttgaacca acatctcga tcagtgttt gcttaaatc accggaaga aagagatgc agaaagtgt tgatcttac tgattcttt ccatgagga ggaagatac aaaagaatg tccactttg taccaaaga aattgtaac ttccaaatt tgacactca tgttgaaaa gtgcctaat ggaggttta taacggagt tgaggaaaa gttggctgt tccaaaagc ccaagaaga aacggtttc gtgtttggc ttgtggtaa aaaaatcgt aaaaagtga acttaattc gcatttgga tgagtgtat ggttgatat tttcgatga tgaggaaag taaagataa ggaagtttg tgataagaa taattcgca gtttaattg tccattttg tttgaaact ggtggaaga gagcgaaat gaaggaaca tattgatgg gtgtttgat ggctgataa tctcgccga ggcttttgc agatgatga tttttgatt gttaattat ttttgtttt tttttatgc caaatatac atacttttt ctaa
