Status
Please wait ...
Gene information
General information
TC032676
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003994,TC003995
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid29: 355574..362078
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032676
    iBeetle screen iB_00648
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    iBeetle screen iB_03665
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva anterior constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: larvae show constrictions only between the head and the thorax
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032676 RA
      MNRSSGDSG QSSSMSYIK SSTFKSVFE LCDVVSVIV 
      CCRADTSIM LIIESSSNE YWIPSIKVT GLNSWLGHL 
      AKDLLNDIF GCNASVQGL MRLNKLWLP SPHKFSYIC 
      HAIFTADVT LEMKKKSKS PFGKYRGKI RWCNLDDIL 
      KLFGSNVLK SPEILDFIA WINRKPSAI LNPPSDHET 
      VGSFMEISE NVIVSGKNT IYEQIVEAA GIDRHAQEV 
      LYKEFVLMS YPAIYLTFT NFAKFVVDL GWTKEEATH 
      LYRSADIYG RGGISFREF LYFLSATEP GTSHGGGPA 
      ELRCRYIFR YLDTDKNNL LKRDEMKNL VILVRKSRK 
      MSLDPTQVE KELVECYQS IGIAETYPL TMSDFLRAV 
      GELKVRGTS QIFRAPNSV QKIIKDIID RDAKSQEES 
      LQPVSIGGY AKCPLYELG IHSVKIGNM INVEQLWSI 
      ENATSCTST LPVSVNETS RKVSTDKFH QNSKSNELL 
      RSLRKLYDL NKKTTCYIK LDSFVYNWG TLDSSVIAK 
      NLASVCSEV KAIFLKERR LLDIPSPVY ILGDLHGNF 
      TDLLNFERI LWHVGPALC PCNLLFLGD YVDRGYFSF 
      EVISYLFSY KIQSPKKVF LLRGNHEIR RVQQQWTFE 
      KECRMKFGE KLGPFIFNS INDAMDCLP LAAVIDGRV 
      FCCHGGIPP PWLCPVVSA INDIPSLLN DPQEQSSLA 
      WELMWNDPI RAKTVTDKI AMELLANEG FAVNAKRGT 
      AHIFSVEAL EKFLASNGF THLVRAHEV VEAGFYLQQ 
      KGHLLTVFS SSKYCGGVN TAACILADQ GKIRILRLD 
      SE
    CDS sequence
    • >TC032676 RA
      atgaatcgt tcgtccggt gattctggc caaagctct 
      tccatgagt tacataaaa tcgtcaact tttaaatcc 
      gtcttcgag ctgtgtgac gtagtttcg gtgatagtt 
      tgttgccga gctgacacc tccataatg ctgatcatt 
      gagtcgagt tccaacgaa tactggatc ccgtccatc 
      aaagttacg gggctcaac agctggctg gggcaccta 
      gccaaggac ttactaaac gatattttc ggctgcaat 
      gcctccgtc caaggcctc atgcgcttg aacaaactg 
      tggttgcct tcgccacac aagttttcc tacatttgc 
      cacgcgatt ttcaccgcc gatgtcaca ctcgagatg 
      aaaaagaaa tcgaaaagt ccattcgga aaatacagg 
      gggaagatc cggtggtgc aatttagac gacattttg 
      aaacttttc ggttctaat gttttgaaa agtcccgaa 
      attttggat ttcattgcg tggattaac cgaaaacct 
      tcggcgatt ttaaacccg ccttcggat cacgaaact 
      gttggcagt tttatggaa atttccgaa aatgttata 
      gtttcgggg aaaaacacg atttatgaa caaattgtg 
      gaagctgca gggatcgac cgtcatgcc caagaagtt 
      ctttacaaa gagttcgtc ctaatgagc tatcccgct 
      atctaccta acttttaca aacttcgcc aaattcgtc 
      gttgattta ggttggacg aaagaagaa gccactcat 
      ttgtaccga tcagcggac atatacgga cgtggggga 
      atttctttt cgtgaattt ttgtatttt ttgtcggcc 
      actgagccg ggaactagc catggggga ggtcccgca 
      gaattgaga tgtcgctac atttttcgg tacttagac 
      actgacaaa aataatctc ctcaaacga gacgagatg 
      aaaaatttg gtgattctc gtgcgaaaa tcaaggaaa 
      atgtccctg gaccccacg caagtggag aaggaattg 
      gtcgagtgt taccaatcg atcggaatc gccgaaaca 
      tatccactc acaatgtct gattttctt cgagctgtt 
      ggtgaattg aaagtacga gggacttcg caaattttt 
      cgcgcacct aatagtgtc caaaaaatc ataaaagat 
      ataattgat cgagacgct aaaagtcag gaagagagt 
      ctccaacct gtttctatt ggtggttat gccaagtgc 
      cctctgtac gagttgggc attcacagt gttaaaatt 
      ggtaatatg atcaacgtc gaacagttg tggagcatt 
      gaaaatgcg acttcttgc acgagcaca cttcccgtc 
      tccgtcaac gaaacttcg cgaaaagta tcaaccgat 
      aaattccac cagaattcc aaatcgaac gaactcctt 
      cgctctcta cgaaaactt tacgacctt aacaaaaaa 
      accacctgt tacattaaa ctcgactct tttgtatac 
      aactgggga acgcttgat tcttcagtt attgccaaa 
      aacttagct tcggtttgt agcgaagtc aaggcaatt 
      tttctgaaa gaacgaaga ttgctagac attccgtca 
      ccagtctac attttaggc gatttacat ggaaatttc 
      actgatttg ctcaacttt gagcgaatt ttatggcac 
      gtgggacct gctttgtgt ccttgtaat cttcttttt 
      ttgggggat tatgttgat cgaggttac ttcagtttc 
      gaggtcatt tcctatttg ttctcctac aagatacaa 
      agtcccaaa aaagtgttt ctgttaaga ggcaaccac 
      gaaattcga cgcgtccaa caacagtgg actttcgaa 
      aaagaatgt agaatgaaa tttggtgaa aagttgggt 
      ccttttatt ttcaattcc ataaacgat gccatggac 
      tgtttgccc cttgctgcg gttattgac ggccgcgtt 
      ttctgctgt catgggggt ataccgccc ccgtggctt 
      tgcccggtt gtttcggca attaacgat attccctcg 
      ttgttgaat gatccacaa gagcaaagt tccctagct 
      tgggaactt atgtggaat gacccaatc cgggcaaaa 
      acagtcact gataaaatc gcaatggaa ttgttggca 
      aatgaggga tttgcggtc aatgccaaa agaggcaca 
      gctcatatt ttcagtgtc gaagctttg gagaagttt 
      ctcgcttcg aatgggttt acgcattta gtgcgagct 
      cacgaggtc gtagaagca ggattttat ttgcagcaa 
      aaaggccac cttttgact gttttttcc tcgtcaaag 
      tattgtgga ggggttaac actgcggcg tgtattctc 
      gccgatcaa ggtaaaatc aggattttg cgacttgac 
      agtgaataa
    mRNA sequence
    • >TC032676 RA
      ttattatta ttaaaaaaa tgtgacata gtgatacgt 
      caaatgttc cgctaaaat gacttttca atgaatcgt 
      tcgtccggt gattctggc caaagctct tccatgagt 
      tacataaaa tcgtcaact tttaaatcc gtcttcgag 
      ctgtgtgac gtagtttcg gtgatagtt tgttgccga 
      gctgacacc tccataatg ctgatcatt gagtcgagt 
      tccaacgaa tactggatc ccgtccatc aaagttacg 
      gggctcaac agctggctg gggcaccta gccaaggac 
      ttactaaac gatattttc ggctgcaat gcctccgtc 
      caaggcctc atgcgcttg aacaaactg tggttgcct 
      tcgccacac aagttttcc tacatttgc cacgcgatt 
      ttcaccgcc gatgtcaca ctcgagatg aaaaagaaa 
      tcgaaaagt ccattcgga aaatacagg gggaagatc 
      cggtggtgc aatttagac gacattttg aaacttttc 
      ggttctaat gttttgaaa agtcccgaa attttggat 
      ttcattgcg tggattaac cgaaaacct tcggcgatt 
      ttaaacccg ccttcggat cacgaaact gttggcagt 
      tttatggaa atttccgaa aatgttata gtttcgggg 
      aaaaacacg atttatgaa caaattgtg gaagctgca 
      gggatcgac cgtcatgcc caagaagtt ctttacaaa 
      gagttcgtc ctaatgagc tatcccgct atctaccta 
      acttttaca aacttcgcc aaattcgtc gttgattta 
      ggttggacg aaagaagaa gccactcat ttgtaccga 
      tcagcggac atatacgga cgtggggga atttctttt 
      cgtgaattt ttgtatttt ttgtcggcc actgagccg 
      ggaactagc catggggga ggtcccgca gaattgaga 
      tgtcgctac atttttcgg tacttagac actgacaaa 
      aataatctc ctcaaacga gacgagatg aaaaatttg 
      gtgattctc gtgcgaaaa tcaaggaaa atgtccctg 
      gaccccacg caagtggag aaggaattg gtcgagtgt 
      taccaatcg atcggaatc gccgaaaca tatccactc 
      acaatgtct gattttctt cgagctgtt ggtgaattg 
      aaagtacga gggacttcg caaattttt cgcgcacct 
      aatagtgtc caaaaaatc ataaaagat ataattgat 
      cgagacgct aaaagtcag gaagagagt ctccaacct 
      gtttctatt ggtggttat gccaagtgc cctctgtac 
      gagttgggc attcacagt gttaaaatt ggtaatatg 
      atcaacgtc gaacagttg tggagcatt gaaaatgcg 
      acttcttgc acgagcaca cttcccgtc tccgtcaac 
      gaaacttcg cgaaaagta tcaaccgat aaattccac 
      cagaattcc aaatcgaac gaactcctt cgctctcta 
      cgaaaactt tacgacctt aacaaaaaa accacctgt 
      tacattaaa ctcgactct tttgtatac aactgggga 
      acgcttgat tcttcagtt attgccaaa aacttagct 
      tcggtttgt agcgaagtc aaggcaatt tttctgaaa 
      gaacgaaga ttgctagac attccgtca ccagtctac 
      attttaggc gatttacat ggaaatttc actgatttg 
      ctcaacttt gagcgaatt ttatggcac gtgggacct 
      gctttgtgt ccttgtaat cttcttttt ttgggggat 
      tatgttgat cgaggttac ttcagtttc gaggtcatt 
      tcctatttg ttctcctac aagatacaa agtcccaaa 
      aaagtgttt ctgttaaga ggcaaccac gaaattcga 
      cgcgtccaa caacagtgg actttcgaa aaagaatgt 
      agaatgaaa tttggtgaa aagttgggt ccttttatt 
      ttcaattcc ataaacgat gccatggac tgtttgccc 
      cttgctgcg gttattgac ggccgcgtt ttctgctgt 
      catgggggt ataccgccc ccgtggctt tgcccggtt 
      gtttcggca attaacgat attccctcg ttgttgaat 
      gatccacaa gagcaaagt tccctagct tgggaactt 
      atgtggaat gacccaatc cgggcaaaa acagtcact 
      gataaaatc gcaatggaa ttgttggca aatgaggga 
      tttgcggtc aatgccaaa agaggcaca gctcatatt 
      ttcagtgtc gaagctttg gagaagttt ctcgcttcg 
      aatgggttt acgcattta gtgcgagct cacgaggtc 
      gtagaagca ggattttat ttgcagcaa aaaggccac 
      cttttgact gttttttcc tcgtcaaag tattgtgga 
      ggggttaac actgcggcg tgtattctc gccgatcaa 
      ggtaaaatc aggattttg cgacttgac agtgaataa 
      aaattgaaa agaaagtgt ttggttgtt t