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Gene information
General information
TC003944 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 27884857..27900136
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC003944
      iBeetle screen iB_03656

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
      Days post injection


      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC003944 RA
        MCDDDVAAL VVDNGSGMC KAGFAGDDA PRAVFPSIV 
        GRPRHQGVM VGMGQKDSY VGDEAQSKR GILTLKYPI 
        EHGIITNWD DMEKIWHHT FYNELRVAP EEHPILLTE 
        APLNPKANR EKMTQIMFE TFNTPAMYV AIQAVLSLY 
        ASGRTTGIV LDSGDGVSH TVPIYEGYA LPHAILRLD 
        LAGRDLTDY LMKILTERG YSFTTTAER EIVRDIKEK 
        LCYVALDFE QEMATAANS TSLEKSYEL PDGQVITIG 
        NERFRCPEA LFQPSFLGM ESCGIHETV YNSIMKCDV 
        DIRKDLYAN TVLSGGTTM YPGIADRMQ KEITALAPS 
        TIKIKIIAP PERKYSVWI GGSILASLS TFQQMWISK 
        QEYDESGPG IVHRKCF
      CDS sequence
      • >TC003944 RA
        atgtgtgac gacgacgta gccgccctc gtggtagac 
        aacggctcc ggcatgtgc aaggccggt ttcgccggt 
        gatgacgcc ccacgtgct gtctttccc tccatcgtg 
        gggcgaccc cgccaccag ggtgtgatg gtgggcatg 
        ggtcagaag gactcctac gtcggagac gaggcccaa 
        agcaagcgc ggtattctc acccttaaa taccccatc 
        gagcacggc atcatcacc aactgggac gacatggag 
        aagatctgg caccacacc ttctacaac gagctgcgc 
        gtggcccca gaggagcat cccatcctc ctcaccgag 
        gccccactc aaccccaaa gccaaccgc gagaagatg 
        acccagatc atgttcgag acttttaat acaccggcc 
        atgtatgtg gccatccag gcggtgctc tcgttgtac 
        gcctctggc cgtaccacc ggcatcgtc ctggactca 
        ggcgatggt gtctcccac acagtcccc atctatgaa 
        ggctacgcc ctcccccat gccatcctt cgcctggac 
        ttggccgga agagactta acagactat ctcatgaaa 
        attttaacc gaaagaggc tactctttc acgaccacc 
        gccgagcgc gaaattgtc agggacatc aaggagaag 
        ctgtgctat gttgcccta gacttcgag caagaaatg 
        gcaactgca gccaactcc acctctctg gagaagagc 
        tacgagttg cccgacggt caggtcatc accatcggg 
        aacgagagg ttcaggtgc cctgaggca ctcttccag 
        ccttcgttc ctcggaatg gaatcgtgc ggcatccac 
        gaaacggtg tacaactcc atcatgaag tgtgatgtt 
        gatatcagg aaagatttg tatgccaat actgtactc 
        tctggtggt accaccatg tacccaggt attgccgac 
        aggatgcag aaggagatc acagccttg gcgccttcg 
        accatcaaa atcaagatt atcgctccg cctgaaaga 
        aagtactca gtctggatc ggtggttca attctggcg 
        tctttgtcc actttccag caaatgtgg atctcgaag 
        caagagtat gatgagtct ggacctgga attgttcat 
        cgtaaatgc ttctaa
      mRNA sequence
      • >TC003944 RA
        ggctataaa aacctccaa gtgcattta actagcact 
        cgatctgca gacggcact tagacgagt ccgaggtgt 
        ggtgttaag tgagtgggt ttttttttg ctgacgatt 
        ttggaacta gttgagtaa taattttct agtaatctc 
        cagccatgt gtgacgacg acgtagccg ccctcgtgg 
        tagacaacg gctccggca tgtgcaagg ccggtttcg 
        ccggtgatg acgccccac gtgctgtct ttccctcca 
        tcgtggggc gaccccgcc accagggtg tgatggtgg 
        gcatgggtc agaaggact cctacgtcg gagacgagg 
        cccaaagca agcgcggta ttctcaccc ttaaatacc 
        ccatcgagc acggcatca tcaccaact gggacgaca 
        tggagaaga tctggcacc acaccttct acaacgagc 
        tgcgcgtgg ccccagagg agcatccca tcctcctca 
        ccgaggccc cactcaacc ccaaagcca accgcgaga 
        agatgaccc agatcatgt tcgagactt ttaatacac 
        cggccatgt atgtggcca tccaggcgg tgctctcgt 
        tgtacgcct ctggccgta ccaccggca tcgtcctgg 
        actcaggcg atggtgtct cccacacag tccccatct 
        atgaaggct acgccctcc cccatgcca tccttcgcc 
        tggacttgg ccggaagag acttaacag actatctca 
        tgaaaattt taaccgaaa gaggctact ctttcacga 
        ccaccgccg agcgcgaaa ttgtcaggg acatcaagg 
        agaagctgt gctatgttg ccctagact tcgagcaag 
        aaatggcaa ctgcagcca actccacct ctctggaga 
        agagctacg agttgcccg acggtcagg tcatcacca 
        tcgggaacg agaggttca ggtgccctg aggcactct 
        tccagcctt cgttcctcg gaatggaat cgtgcggca 
        tccacgaaa cggtgtaca actccatca tgaagtgtg 
        atgttgata tcaggaaag atttgtatg ccaatactg 
        tactctctg gtggtacca ccatgtacc caggtattg 
        ccgacagga tgcagaagg agatcacag ccttggcgc 
        cttcgacca tcaaaatca agattatcg ctccgcctg 
        aaagaaagt actcagtct ggatcggtg gttcaattc 
        tggcgtctt tgtccactt tccagcaaa tgtggatct 
        cgaagcaag agtatgatg agtctggac ctggaattg 
        ttcatcgta aatgcttct aaagttaga gttttatga 
        ctgtggcaa gcactgccg gatgatttc tattgaatt 
        ataagactt gtgtaccta cacctctcc gataattaa 
        tttattcct aagagtatt gtaatttgt agttatttg 
        tatcgataa tttaattac ctgtaaaaa ttaatgtag 
        cgaaaatgg aaaattatt atctaataa agtgttttt 
        attaaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY