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Gene information
General information
TC034633 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid26: 652823..702403
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034633
    iBeetle screen iB_03652
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition >5 number 2nd lateral oviduct: number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 5
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    iBeetle screen iB_03653
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    remarks: later than schedule, hours 48 adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    remarks: later than schedule, hours 48 remarks: later than schedule, hours 48
    Pupal morphology
    remarks: later than schedule, hours 48
    Adult morphology
    remarks: later than schedule, hours 48 remarks: later than schedule, hours 48
    Fertility
    mature egg deposition >5 number 2nd lateral oviduct: number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    remarks: later than schedule, hours 48 (egglay)
    Analysis of larval stage 1 cuticle
    remarks: later than schedule, hours 48
    remarks - cuticles: later than schedule, hours 48
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034633 RA
      MTVTYTGQV PNGSTFGCF WKVLGIWRG SVYKLVWRE 
      LVAYLFLYY SINFLYRFG LNQDQKKIF ERVQLYFSQ 
      HTETIPMSF VLGFYVTLV VKRWWEQYR LLPWPDTLA 
      LFLNAGIPG ADERQRLMR RNVVRYAVL AYVITLQRV 
      SLRVRKRFP THQHIVDSG LMLESEKKI FELMDTKTP 
      MSKYWMPLV WATNLIIRA RKENLINSD QLVQTILME 
      LSEIRRRLG SLIGYDTVC VPLVYTQVV SLALYLYFI 
      AALLGRQFV AVGPPKEVT TKEHPDMYF PFFTAIQFC 
      FYVGWLKVA EVLINPFGE DDDDIELNW LIDRHIKAA 
      YMIVDEMHE EHPELLKDQ YWDEVVPKE LPYTVASEH 
      YRREEPKGS AEMYKIKTT DGLYANLLA PKKSLIHDD 
      MYADYESVD TPIVERRKS SNWFTRQIS RTGMGSIRS 
      ASTAYSSGG LFGRHRGNS VYANPENGQ LPGATPPQK 
      MSIYDRLVG RKSGRNQKK QGSRFSGAA PMQVRNRPR 
      IPTPDVTKE VVDRENRMA AANVANIQN QMSHGITLM 
      PHLQTAYPT YPSGDGPVV QVVLSPIQE LDGSNSVNN 
      TLHPHQPGT AALAQAVLS PGLGPVLTT TPVSVPMTV 
      SQLTSLGLT SVHNSPLMA RNEPEAKKT SFISVFEGE 
      KGANQPLVL TELTPGEEE VQLTSRSSD VSEASSSGS 
      KESKNSTAT ASMGAVAET SLTPSNDGS RSRKTSVVS 
      QNNVNNNSS KRGEVYV
    CDS sequence
    • >TC034633 RA
      atgactgtt acatacacg ggacaagtt cctaacgga 
      agtactttc ggttgtttt tggaaagta ctaggaata 
      tggagaggc agtgtgtac aaattagtg tggcgggag 
      ctagtggcc tatttattt ctatactat tccatcaat 
      tttttatat aggtttggg ctaaatcaa gaccaaaaa 
      aaaatattt gaaagggta caactgtac tttagccaa 
      cacactgag acaatacca atgtcattt gtattagga 
      ttctatgta acattggta gtgaagagg tggtgggaa 
      caatataga ctgttgccg tggcctgat actcttgct 
      ctgtttttg aatgcgggc attccgggt gcggatgaa 
      agacaaaga ctgatgaga cgaaacgta gttagatat 
      gcagtttta gcctatgtg ataacactc cagagggtt 
      tcgttacga gtgaggaaa cggtttcca actcatcaa 
      catattgtg gattccggt ctcatgttg gaaagtgag 
      aagaaaatc ttcgagctg atggacacc aaaacaccc 
      atgtccaag tactggatg cccttggtc tgggcaacg 
      aatcttatc ataagggcg aggaaggaa aatctgata 
      aatagcgac caattggtc caaaccatt ttgatggaa 
      ttatcggaa ataagacga cgtctgggc tccttgatc 
      ggttatgat acggtgtgt gtgcccctg gtttacaca 
      caggtcgtt tccttagca ttatatctc tacttcata 
      gcggctttg ctgggccgc caattcgtt gcggtcgga 
      cctccaaaa gaagtaaca acaaaggaa catccagac 
      atgtacttc ccttttttc accgcaata caattttgt 
      ttttacgtt ggatggctc aaagtagcc gaagtattg 
      attaatccc tttggcgaa gacgatgac gatattgaa 
      ttgaattgg ctcattgac agacacatc aaggctgct 
      tacatgatt gtcgatgaa atgcacgag gagcatccc 
      gaattgtta aaagaccag tactgggat gaagtggta 
      ccgaaagaa ttaccatat acggtggct tcagaacac 
      tatcgaaga gaggaaccc aaaggctca gccgaaatg 
      tacaaaatc aagaccaca gatgggcta tatgccaat 
      ttattggct cccaaaaaa tcgctgatt catgacgac 
      atgtatgcc gattacgaa agtgtggac actccgatt 
      gtggagaga aggaaaagc tccaattgg ttcacccgt 
      cagatttcc aggaccggc atgggctca attcgttcc 
      gcctcaacg gcttattct tccggaggg ttgtttggt 
      cgacatcgg ggtaattcg gtttatgcc aaccctgaa 
      aatgggcaa ttaccgggg gccacccca ccgcaaaaa 
      atgtccatt tacgatcga ttagttggg cgaaaatcg 
      ggccggaat cagaaaaaa caaggctca agattcagc 
      ggcgccgct cccatgcaa gtccgcaac cgcccccgc 
      atccccacc cccgacgtg accaaagaa gtggtcgac 
      cgtgagaac cgcatggct gccgccaac gtggccaac 
      atccagaac cagatgtcg cacggcatc accctcatg 
      ccccacctc cagaccgcc taccccacc taccccagc 
      ggcgacggc cccgtggtc caggtggtc ctctccccc 
      atccaggaa ctggacggc tccaactcc gtcaacaac 
      accctccac ccccaccag cctggcacc gccgccctg 
      gcccaagcc gtgctatcc ccaggtctg gggccagtc 
      ctgaccaca acccccgtc agtgttccc atgacggtt 
      tcacagctg acgagcttg gggttgact tcggtgcat 
      aacagcccc ctgatggct cggaatgag ccggaggcg 
      aagaaaacg agctttatt tccgttttt gagggagaa 
      aaaggggcg aatcagccg ctggtcttg acggagttg 
      accccaggg gaggaggag gtgcagttg acgagtcgg 
      agtagtgac gtgtcggag gcgtcgtcg agtggctcc 
      aaggagagt aagaattcg acggcgacg gcgtccatg 
      ggggctgtg gcagagacg tcgctaacg ccctcgaat 
      gatggctcc aggagtagg aaaacgtcg gtggtttcg 
      cagaataat gttaataat aattcgtcg aaaaggggc 
      gaagtgtat gtgtga
    mRNA sequence
    • >TC034633 RA
      gtcagtttg taacaacta gtcgtctag tgacgtttt 
      cgttagtac tttgaaaca tcattagct tccaatgtc 
      cataatttc ggtcgcaaa taagtgaag tgtgtgcgt 
      gttgtggtg atgtgtagt tttgcttgt ttgtggtaa 
      aatgactgt tacatacac gggacaagt tcctaacgg 
      aagtacttt cggttgttt ttggaaagt actaggaat 
      atggagagg cagtgtgta caaattagt gtggcggga 
      gctagtggc ctatttatt tctatacta ttccatcaa 
      ttttttata taggtttgg gctaaatca agaccaaaa 
      aaaaatatt tgaaagggt acaactgta ctttagcca 
      acacactga gacaatacc aatgtcatt tgtattagg 
      attctatgt aacattggt agtgaagag gtggtggga 
      acaatatag actgttgcc gtggcctga tactcttgc 
      tctgttttt gaatgcggg cattccggg tgcggatga 
      aagacaaag actgatgag acgaaacgt agttagata 
      tgcagtttt agcctatgt gataacact ccagagggt 
      ttcgttacg agtgaggaa acggtttcc aactcatca 
      acatattgt ggattccgg tctcatgtt ggaaagtga 
      gaagaaaat cttcgagct gatggacac caaaacacc 
      catgtccaa gtactggat gcccttggt ctgggcaac 
      gaatcttat cataagggc gaggaagga aaatctgat 
      aaatagcga ccaattggt ccaaaccat tttgatgga 
      attatcgga aataagacg acgtctggg ctccttgat 
      cggttatga tacggtgtg tgtgcccct ggtttacac 
      acaggtcgt ttccttagc attatatct ctacttcat 
      agcggcttt gctgggccg ccaattcgt tgcggtcgg 
      acctccaaa agaagtaac aacaaagga acatccaga 
      catgtactt ccctttttt caccgcaat acaattttg 
      tttttacgt tggatggct caaagtagc cgaagtatt 
      gattaatcc ctttggcga agacgatga cgatattga 
      attgaattg gctcattga cagacacat caaggctgc 
      ttacatgat tgtcgatga aatgcacga ggagcatcc 
      cgaattgtt aaaagacca gtactggga tgaagtggt 
      accgaaaga attaccata tacggtggc ttcagaaca 
      ctatcgaag agaggaacc caaaggctc agccgaaat 
      gtacaaaat caagaccac agatgggct atatgccaa 
      tttattggc tcccaaaaa atcgctgat tcatgacga 
      catgtatgc cgattacga aagtgtgga cactccgat 
      tgtggagag aaggaaaag ctccaattg gttcacccg 
      tcagatttc caggaccgg catgggctc aattcgttc 
      cgcctcaac ggcttattc ttccggagg gttgtttgg 
      tcgacatcg gggtaattc ggtttatgc caaccctga 
      aaatgggca attaccggg ggccacccc accgcaaaa 
      aatgtccat ttacgatcg attagttgg gcgaaaatc 
      gggccggaa tcagaaaaa acaaggctc aagattcag 
      cggcgccgc tcccatgca agtccgcaa ccgcccccg 
      catccccac ccccgacgt gaccaaaga agtggtcga 
      ccgtgagaa ccgcatggc tgccgccaa cgtggccaa 
      catccagaa ccagatgtc gcacggcat caccctcat 
      gccccacct ccagaccgc ctaccccac ctaccccag 
      cggcgacgg ccccgtggt ccaggtggt cctctcccc 
      catccagga actggacgg ctccaactc cgtcaacaa 
      caccctcca cccccacca gcctggcac cgccgccct 
      ggcccaagc cgtgctatc cccaggtct ggggccagt 
      cctgaccac aacccccgt cagtgttcc catgacggt 
      ttcacagct gacgagctt ggggttgac ttcggtgca 
      taacagccc cctgatggc tcggaatga gccggaggc 
      gaagaaaac gagctttat ttccgtttt tgagggaga 
      aaaaggggc gaatcagcc gctggtctt gacggagtt 
      gaccccagg ggaggagga ggtgcagtt gacgagtcg 
      gagtagtga cgtgtcgga ggcgtcgtc gagtggctc 
      caaggagag taagaattc gacggcgac ggcgtccat 
      gggggctgt ggcagagac gtcgctaac gccctcgaa 
      tgatggctc caggagtag gaaaacgtc ggtggtttc 
      gcagaataa tgttaataa taattcgtc gaaaagggg 
      cgaagtgta tgtgtgaag gggactcct gtttgaggg 
      atcgaagcg ttgggagtg gacggaaat attgtttca 
      gatgtgtta aagatgggg gttaagtga tttatcgga 
      tgaaataaa caaactata aaaaaatga ataagacaa 
      aaattgaaa tttggcaaa aatgcgatt ttgtttgtc 
      tctcttata cacattttc gaaagaagt ttatgtcgt 
      ttgtcactt ttaaattaa tacatacaa tgaaaaaaa 
      taaaagaat acaaagata attaaataa aagaaacaa 
      atttttgga aaggcttat cgaagaaca gtattcaaa 
      cgtttctgc attacacgt atttacata taattctgt 
      agataatac aaaatgaca cattgtaat tcaaacgga 
      aataaataa ataaaaatt ttgtgataa taaaactta 
      aaagaaatt tggaatggg tgtgtgtat tttatttat 
      gttttttac aaatgtata gttaattat cgaaatttt 
      taataaatt aaacgtgtc gttatacaa acaaacaaa 
      agacaaaac ttgggaaga aaaagaacg aaaaagcac 
      aaattcttt attggcttt gcaaaactt cacaatata 
      tcatgtgat gtatatttt ttattgatt taatgattt 
      attaaataa atgtaagca ataaccaaa accttccac 
      ggaaacatc ttacaagaa aaatgcctg aaacattaa 
      aaaaaatca taatctcta aaaagcgcc aatttttct 
      ttgttttag acaacttat agtttgaat aaaataaca 
      tattgtgag aacaattat caaaaaaat acacaaaag 
      tgattgtat ttttcagct ttcccttat attttgtca 
      cattcatta aaatgcaat aaatataag acacacaga 
      aataaaann nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnntatcaa aaaaataca 
      gaaaagtga ttgtatttt tcagctttc ccttatatt 
      ttgtcagat tcattaaaa tgcaataaa tataagaca 
      cacagaaat aaaagaaag tctaggaaa aataataaa 
      tagttgctg agaagttca ataaaacac tgaaaaagt 
      gtttttttt tttaatttc tttacattt tttcactag 
      atgtttgtt acttgtatc gctaacttt acaaataaa 
      ttataaaca aactgtaag aaaaacata aaaaaacat 
      aagtaaaac ataaaaaca acaaaatct acgaagaag 
      acgttcttt tcttaacaa attttcaca aaactttcc 
      gtgaattat agattgagg aaaagaaaa tcaaaaagt 
      aataatatt aaaatagac caaaaattg ttggaaaaa 
      tattaaaat actgaaaca cttaatact tacgaacga 
      gaaaccaaa tagaacaaa aattatgga ggaaaataa 
      aattgttaa ttaatacta aaaaaagaa taaaaaaag 
      agatgaaga aataattga agatgaaaa atacagaaa 
      cttaaaaaa gtgcttttt gtcgttccc ttgaaatcc 
      ttgaacatt ctctgaaaa aattgaaat cttcttaca 
      caatttatt gtgtgtaaa ataaattaa atgctaaaa 
      aaatcgatg aaaaatgaa cttttaacc aaaaattct 
      cgcgacaaa aagttatta gaacttttc aaaaaaagg 
      aggaattcg aaaaactat agaaaaaag tcggaattt 
      taattaaaa ctttaaaaa tttg