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Gene information
General information
TC003908 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 26565704..26612658
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003908
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03648

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature mostly fluorescence not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: no fluorescence, because larvae are dead
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    legs tibiotarsus shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs tibiotarsus potentially fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs femur potentially fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs segment cuticularisation decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003908 RA
      MDSGDCETE EDSAPEACV SPEGSLLPS NSREMRNRA 
      EKMRRDKLN SYIGELATL VPMVARSAK RMDKTSILR 
      LAATHLRIY QTLLSGKNH PHIQLPKHV DQYLLEQLV 
      CEQLGGFLL ILTPNGKIV FVSHTVEHL LGHLQTDLM 
      GQSIFNITS PDDHDRLRM YINTESVLD GDWKKCFNI 
      RLKRAGPRT ESAVYEPVR IMGVHRPGF DNDCNKNTS 
      TSKEIALNN DVLLFFVKV FRPEPLCER LFEASREEY 
      VTRHLIDGR IIGCDQRIS FIAGYMTEE VSGLSAFKF 
      MHREDVRWV MIALRQMYD RGESKGSSC YRLLSRNGQ 
      FIYLRTFGF LEIDDQGTV ESFVCVNTL VSEQEGLQL 
      INEMKKRYS ALINSQSCP ITSSGSTDS SSQSVEDPQ 
      QVEAAIVHL IANLPSPGS DQRSTPSPR VYGNVNENQ 
      DCSTPTENS PTKPYYKLK TANNKRPPS TELGTNIYT 
      SSKRQRTSP QLSPMSSLP PYPNRTQIT EVTQQTEPS 
      IYQHDQLLR NKV
    • >TC003908 RB
      MFHLYVHLF FSQNKRKIV ALIRVRTPD PSGREPPTM 
      DSGDCETEE DSAPEACVS PEGSLLPSN SREMRNRAE 
      KMRRDKLNS YIGELATLV PMVARSAKR MDKTSILRL 
      AATHLRIYQ TLLSGKNHP HIQLPKHVD QYLLEQLVC 
      EQLGGFLLI LTPNGKIVF VSHTVEHLL GHLQTDLMG 
      QSIFNITSP DDHDRLRMY INTESVLDG DWKKCFNIR 
      LKRAGPRTE SAVYEPVRI MGVHRPGFD NDCNKNTST 
      SKEIALNND VLLFFVKVF RPEPLCERL FEASREEYV 
      TRHLIDGRI IGCDQRISF IAGYMTEEV SGLSAFKFM 
      HREDVRWVM IALRQMYDR GESKGSSCY RLLSRNGQF 
      IYLRTFGFL EIDDQGTVE SFVCVNTLV SEQEGLQLI 
      NEMKKRYSA LINSQSCPI TSSGSTDSS SQSVEDPQQ 
      VEAAIVHLI ANLPSPGSD QRSTPSPRV YGNVNENQD 
      CSTPTENSP TKPYYKLKT ANNKRPPST ELGTNIYTS 
      SKRQRTSPQ LSPMSSLPP YPNRTQITE VTQQTEPSI 
      YQHDQLLRN KV
    CDS sequence
    • >TC003908 RA
      atggacagt ggagattgc gagactgaa gaagattca 
      gcaccagag gcgtgcgtt tctcctgaa ggttctctg 
      ttgccaagc aatagccgg gagatgagg aacagggcg 
      gaaaagatg cgaagggac aaactgaat tcgtacatt 
      ggggaattg gcaactttg gttccgatg gtcgcaaga 
      agtgccaaa agaatggac aagacgagc attcttcga 
      ctagcggcc acacatctg cgaatatat caaaccttg 
      ctgagtgga aagaaccat ccgcacatc cagttgccg 
      aaacatgtt gatcaatat ttattagaa caactggta 
      tgcgagcag ttgggtggt tttctgcta atcctgacg 
      cccaatgga aaaatagtg tttgtctcc cacactgtg 
      gagcatctt ttgggccac ttacagact gatttgatg 
      ggtcaatca atttttaat ataacgtca ccagatgac 
      cacgaccgc ctgcgtatg tacataaac acggagagt 
      gttctagac ggagactgg aaaaaatgc ttcaatatt 
      cgactgaaa agggccggc cctaggact gaatcggcc 
      gtttatgag cccgtcaga atcatgggc gtacataga 
      ccaggcttc gacaatgac tgcaacaaa aatacatcg 
      actagcaaa gaaatcgca ttgaataac gatgtgctg 
      ctctttttt gtcaaagtt ttccgtccg gagccactt 
      tgtgaacga cttttcgaa gcttcaaga gaggaatat 
      gtaaccaga catttgatt gacggccgg ataattggc 
      tgcgatcag cgaatttca ttcattgca ggttatatg 
      actgaggaa gtgtccgga ttatcggcg ttcaaattc 
      atgcatcgg gaggatgtt cgatgggtg atgatcgcc 
      ttaagacaa atgtatgac cgaggggaa agcaaagga 
      tcatcatgt taccgttta ctctcgagg aatggacag 
      tttatctat ttaaggacg ttcggtttt ctagaaata 
      gacgaccag ggaactgtt gaaagtttt gtttgtgtt 
      aacacttta gtgagtgag caagaaggc cttcaatta 
      ataaacgaa atgaagaag cggtactct gccttgatt 
      aattcacag agttgccca atcacttca agtggctca 
      accgactcg tcttcccag tcggttgaa gacccccaa 
      caggttgaa gcggcgatc gtgcacctc atagccaac 
      ttgccatcg ccgggctca gaccagcgc tcaaccccc 
      agccctcga gtttatggt aatgttaat gagaatcaa 
      gactgctca acaccgact gaaaattcc ccaaccaaa 
      ccatactac aaattaaag actgcaaac aacaaacgc 
      ccgcccagt actgaatta ggaaccaac atttatacg 
      tcgagtaaa cgacagagg acttcgcct caactctca 
      ccaatgtcg agtttgccg ccttacccg aaccgaact 
      caaatcacg gaagtaaca caacaaacc gaaccgtcg 
      atatatcag catgaccaa ctacttcgt aacaaagta 
      tga
    • >TC003908 RB
      atgtttcac ctttacgtt catctcttt ttctctcaa 
      aacaaaaga aaaattgtc gccctgatc cgggttcga 
      acacccgac ccctctggt cgtgaaccg ccaaccatg 
      gacagtgga gattgcgag actgaagaa gattcagca 
      ccagaggcg tgcgtttct cctgaaggt tctctgttg 
      ccaagcaat agccgggag atgaggaac agggcggaa 
      aagatgcga agggacaaa ctgaattcg tacattggg 
      gaattggca actttggtt ccgatggtc gcaagaagt 
      gccaaaaga atggacaag acgagcatt cttcgacta 
      gcggccaca catctgcga atatatcaa accttgctg 
      agtggaaag aaccatccg cacatccag ttgccgaaa 
      catgttgat caatattta ttagaacaa ctggtatgc 
      gagcagttg ggtggtttt ctgctaatc ctgacgccc 
      aatggaaaa atagtgttt gtctcccac actgtggag 
      catcttttg ggccactta cagactgat ttgatgggt 
      caatcaatt tttaatata acgtcacca gatgaccac 
      gaccgcctg cgtatgtac ataaacacg gagagtgtt 
      ctagacgga gactggaaa aaatgcttc aatattcga 
      ctgaaaagg gccggccct aggactgaa tcggccgtt 
      tatgagccc gtcagaatc atgggcgta catagacca 
      ggcttcgac aatgactgc aacaaaaat acatcgact 
      agcaaagaa atcgcattg aataacgat gtgctgctc 
      ttttttgtc aaagttttc cgtccggag ccactttgt 
      gaacgactt ttcgaagct tcaagagag gaatatgta 
      accagacat ttgattgac ggccggata attggctgc 
      gatcagcga atttcattc attgcaggt tatatgact 
      gaggaagtg tccggatta tcggcgttc aaattcatg 
      catcgggag gatgttcga tgggtgatg atcgcctta 
      agacaaatg tatgaccga ggggaaagc aaaggatca 
      tcatgttac cgtttactc tcgaggaat ggacagttt 
      atctattta aggacgttc ggttttcta gaaatagac 
      gaccaggga actgttgaa agttttgtt tgtgttaac 
      actttagtg agtgagcaa gaaggcctt caattaata 
      aacgaaatg aagaagcgg tactctgcc ttgattaat 
      tcacagagt tgcccaatc acttcaagt ggctcaacc 
      gactcgtct tcccagtcg gttgaagac ccccaacag 
      gttgaagcg gcgatcgtg cacctcata gccaacttg 
      ccatcgccg ggctcagac cagcgctca acccccagc 
      cctcgagtt tatggtaat gttaatgag aatcaagac 
      tgctcaaca ccgactgaa aattcccca accaaacca 
      tactacaaa ttaaagact gcaaacaac aaacgcccg 
      cccagtact gaattagga accaacatt tatacgtcg 
      agtaaacga cagaggact tcgcctcaa ctctcacca 
      atgtcgagt ttgccgcct tacccgaac cgaactcaa 
      atcacggaa gtaacacaa caaaccgaa ccgtcgata 
      tatcagcat gaccaacta cttcgtaac aaagtatga
    mRNA sequence
    • >TC003908 RA
      tgggaaaga aacctgtct aaaccgttc tctgtttcg 
      gctgattcc cctagatgg cgaaacgtt cgcaaaaat 
      aataaaata tacagagtg gtgtgcaac atacgcgta 
      gaggtgttt tccaagtgc aattttcta ttatttcca 
      tttattgca ttactttca aagctaaaa caataagtg 
      ataaatttt ttacgttca aaagagcca taacttttc 
      tacttacac gtcgttgaa ataaaactg gcaacgaaa 
      gttgcattt taaaggatg aaagtagag acatctttc 
      attcgtttc tcacgaaga gtatggtgg attgtaaac 
      ttgtgatgt gtttaaatg gtttgagat gatgttaat 
      tgtatcaaa caacgattt caatgtgct gtgactatt 
      ttttatcgt agtgaatgt agtttttcg acacgagac 
      tgcttttaa agactggag catattttg atctatctg 
      gtgaaccgc caaccatgg acagtggag attgcgaga 
      ctgaagaag attcagcac cagaggcgt gcgtttctc 
      ctgaaggtt ctctgttgc caagcaata gccgggaga 
      tgaggaaca gggcggaaa agatgcgaa gggacaaac 
      tgaattcgt acattgggg aattggcaa ctttggttc 
      cgatggtcg caagaagtg ccaaaagaa tggacaaga 
      cgagcattc ttcgactag cggccacac atctgcgaa 
      tatatcaaa ccttgctga gtggaaaga accatccgc 
      acatccagt tgccgaaac atgttgatc aatatttat 
      tagaacaac tggtatgcg agcagttgg gtggttttc 
      tgctaatcc tgacgccca atggaaaaa tagtgtttg 
      tctcccaca ctgtggagc atcttttgg gccacttac 
      agactgatt tgatgggtc aatcaattt ttaatataa 
      cgtcaccag atgaccacg accgcctgc gtatgtaca 
      taaacacgg agagtgttc tagacggag actggaaaa 
      aatgcttca atattcgac tgaaaaggg ccggcccta 
      ggactgaat cggccgttt atgagcccg tcagaatca 
      tgggcgtac atagaccag gcttcgaca atgactgca 
      acaaaaata catcgacta gcaaagaaa tcgcattga 
      ataacgatg tgctgctct tttttgtca aagttttcc 
      gtccggagc cactttgtg aacgacttt tcgaagctt 
      caagagagg aatatgtaa ccagacatt tgattgacg 
      gccggataa ttggctgcg atcagcgaa tttcattca 
      ttgcaggtt atatgactg aggaagtgt ccggattat 
      cggcgttca aattcatgc atcgggagg atgttcgat 
      gggtgatga tcgccttaa gacaaatgt atgaccgag 
      gggaaagca aaggatcat catgttacc gtttactct 
      cgaggaatg gacagttta tctatttaa ggacgttcg 
      gttttctag aaatagacg accagggaa ctgttgaaa 
      gttttgttt gtgttaaca ctttagtga gtgagcaag 
      aaggccttc aattaataa acgaaatga agaagcggt 
      actctgcct tgattaatt cacagagtt gcccaatca 
      cttcaagtg gctcaaccg actcgtctt cccagtcgg 
      ttgaagacc cccaacagg ttgaagcgg cgatcgtgc 
      acctcatag ccaacttgc catcgccgg gctcagacc 
      agcgctcaa cccccagcc ctcgagttt atggtaatg 
      ttaatgaga atcaagact gctcaacac cgactgaaa 
      attccccaa ccaaaccat actacaaat taaagactg 
      caaacaaca aacgcccgc ccagtactg aattaggaa 
      ccaacattt atacgtcga gtaaacgac agaggactt 
      cgcctcaac tctcaccaa tgtcgagtt tgccgcctt 
      acccgaacc gaactcaaa tcacggaag taacacaac 
      aaaccgaac cgtcgatat atcagcatg accaactac 
      ttcgtaaca aagtatgaa acgctttgt gtacttgga 
      gactgaaaa ctgggccat ctgttggcc gatttatgc 
      ctcaaggac tatttagaa ggtgtggtg atgtttaat 
      gatcatgtg ttaaccgct aacctgaca cgtaaggca 
      gattcaatt tattttacg attgaatta ttccgattc 
      gaatttgaa atgtgccca aggcacatt tcaaattcg 
      accgtttcg tcgagtgaa cagactgta catgtaaac 
      gttgtatag attttgcat attctcgta atataccta 
      tattaaatt aaatagtaa ttctgtgat gaaattatg 
      tttacgtta cttttaagc agtgtcttt tgtatattt 
      agtacaaat tcaaaaatt tctttattt tattttatg 
      tattactca cgtcccgtt tgctgcaag ccgtctcac 
      cgataactg cagtgttgt aacaaacgg gagccaatc 
      atcgacaac aaaatactt gtatgacat tatgccgcg 
      aaggtttca tgtgaatgt aggtatttc atattcata 
      tttaccatt tatttatca agttgaata tacatagcc 
      gtaattttt atatgctgc agttttagg tttttattg 
      tgaaataaa cgagttttg tttttggat gaagcttta 
      tttggttgt aaataaaag tattttgtt gttttttgt 
      taagtgtgc cctttgtgg ggcgtcaag cgaagcacc 
      ctcgcacag atttgaact gattgaaaa aacaaagga 
      aattaattt acttaacca cttggataa gcaaattac 
      cgctgaaga atctttgtc actattgtg tacttttga 
      ataaattcg aaaaacaaa cggtcaatg ccttcaaat 
      ttttaaata tgtgtacca tgtattctc gataatctg 
      tctttaata gaaaagaaa aattcttgc aaaaatcat 
      ggaaaaaat attttttat ttacgaggc gatgccgag 
      tatggaggc acacattga ataagttac tttatccca 
      gtgttgtct tgcctttat tcagtaata ataataata 
      aaacaaaaa tgataacaa gtagaaatt tttttcttc 
      gctccatat tgttttatt tttgaaaaa gttaatttt 
      tctagccat cgtttcaaa tgatttagg gttgtttta 
      aaaaaaatt agttgtcta caattttgg cccttatga 
      cattcataa taatgaatt tttgaatgt gtttgttgc 
      ccctgaaaa aatatgaaa cgaatttta ttatacagg 
      gtacctaac tcacataat atgacaagc atacaaggt 
      gtgtcgcct aaaagttta gttttacta tacttttct 
      tatctcggt ctaaaagta acaaaattt aaagttttt 
      ttgtgcaaa aaattcaaa aaaaaattg gcgccaacc 
      ccaaaaaat tattaaata aagatttaa ccaacgtat 
      tttttgggc tagagataa ttttttatg ttttttttg 
      cacaagata ttaagcatc gtttgattc aaaaattct 
      gaaaaaaac tttcggcag aacgcttga attatttaa 
      aaaatacat taatcaatt aggtgagat gtaaagctt 
      gacttttta caagaatta cctgtagtt tttggtaaa 
      atatttgct ataattttt atttcttat tctgaagta 
      ttattttat tattcctct gtctgtaat ttgtctgtt 
      tatttttct tcgcccttt ttcaaaatt ttgttgctc 
      ccaaactat aataattga atgacattt tt
    • >TC003908 RB
      tgggaaaga aacctgtct aaaccgttc tctgtttcg 
      gctgattcc cctagatgg cgaaacgtt cgcaaaaat 
      aataaaata tacagagtg gtgtgcaac atacgcgta 
      gaggtgttt tccaagtgc aattttcta ttatttcca 
      tttattgca ttactttca aagctaaaa caataagtg 
      ataaatttt ttacgttca aaagagcca taacttttc 
      tacttacac gtcgttgaa ataaaactg gcaacgaaa 
      gttgcattt taaaggatg aaagtagag acatctttc 
      attcgtttc tcacgaaga gtatggtgg attgtaaac 
      ttgtgatgt gtttaaatg gtttgagat gatgttaat 
      tgtatcaaa caacgattt caatgtgct gtgactatt 
      ttttatcgt agtgaatgt agtttttcg acacgagac 
      tgcttttaa agactggag catattttg atctatctg 
      gtaagtcaa catgctttc acgttgatt cagtgctaa 
      agtttgaag ttatgtttc acctttacg ttcatctct 
      ttttctctc aaaacaaaa gaaaaattg tcgccctga 
      tccgggttc gaacacccg acccctctg gtcgtgaac 
      cgccaacca tggacagtg gagattgcg agactgaag 
      aagattcag caccagagg cgtgcgttt ctcctgaag 
      gttctctgt tgccaagca atagccggg agatgagga 
      acagggcgg aaaagatgc gaagggaca aactgaatt 
      cgtacattg gggaattgg caactttgg ttccgatgg 
      tcgcaagaa gtgccaaaa gaatggaca agacgagca 
      ttcttcgac tagcggcca cacatctgc gaatatatc 
      aaaccttgc tgagtggaa agaaccatc cgcacatcc 
      agttgccga aacatgttg atcaatatt tattagaac 
      aactggtat gcgagcagt tgggtggtt ttctgctaa 
      tcctgacgc ccaatggaa aaatagtgt ttgtctccc 
      acactgtgg agcatcttt tgggccact tacagactg 
      atttgatgg gtcaatcaa tttttaata taacgtcac 
      cagatgacc acgaccgcc tgcgtatgt acataaaca 
      cggagagtg ttctagacg gagactgga aaaaatgct 
      tcaatattc gactgaaaa gggccggcc ctaggactg 
      aatcggccg tttatgagc ccgtcagaa tcatgggcg 
      tacatagac caggcttcg acaatgact gcaacaaaa 
      atacatcga ctagcaaag aaatcgcat tgaataacg 
      atgtgctgc tcttttttg tcaaagttt tccgtccgg 
      agccacttt gtgaacgac ttttcgaag cttcaagag 
      aggaatatg taaccagac atttgattg acggccgga 
      taattggct gcgatcagc gaatttcat tcattgcag 
      gttatatga ctgaggaag tgtccggat tatcggcgt 
      tcaaattca tgcatcggg aggatgttc gatgggtga 
      tgatcgcct taagacaaa tgtatgacc gaggggaaa 
      gcaaaggat catcatgtt accgtttac tctcgagga 
      atggacagt ttatctatt taaggacgt tcggttttc 
      tagaaatag acgaccagg gaactgttg aaagttttg 
      tttgtgtta acactttag tgagtgagc aagaaggcc 
      ttcaattaa taaacgaaa tgaagaagc ggtactctg 
      ccttgatta attcacaga gttgcccaa tcacttcaa 
      gtggctcaa ccgactcgt cttcccagt cggttgaag 
      acccccaac aggttgaag cggcgatcg tgcacctca 
      tagccaact tgccatcgc cgggctcag accagcgct 
      caaccccca gccctcgag tttatggta atgttaatg 
      agaatcaag actgctcaa caccgactg aaaattccc 
      caaccaaac catactaca aattaaaga ctgcaaaca 
      acaaacgcc cgcccagta ctgaattag gaaccaaca 
      tttatacgt cgagtaaac gacagagga cttcgcctc 
      aactctcac caatgtcga gtttgccgc cttacccga 
      accgaactc aaatcacgg aagtaacac aacaaaccg 
      aaccgtcga tatatcagc atgaccaac tacttcgta 
      acaaagtat gaaacgctt tgtgtactt ggagactga 
      aaactgggc catctgttg gccgattta tgcctcaag 
      gactattta gaaggtgtg gtgatgttt aatgatcat 
      gtgttaacc gctaacctg acacgtaag gcagattca 
      atttatttt acgattgaa ttattccga ttcgaattt 
      gaaatgtgc ccaaggcac atttcaaat tcgaccgtt 
      tcgtcgagt gaacagact gtacatgta aacgttgta 
      tagattttg catattctc gtaatatac ctatattaa 
      attaaatag taattctgt gatgaaatt atgtttacg 
      ttactttta agcagtgtc ttttgtata tttagtaca 
      aattcaaaa atttcttta ttttatttt atgtattac 
      tcacgtccc gtttgctgc aagccgtct caccgataa 
      ctgcagtgt tgtaacaaa cgggagcca atcatcgac 
      aacaaaata cttgtatga cattatgcc gcgaaggtt 
      tcatgtgaa tgtaggtat ttcatattc atatttacc 
      atttattta tcaagttga atatacata gccgtaatt 
      tttatatgc tgcagtttt aggttttta ttgtgaaat 
      aaacgagtt ttgtttttg gatgaagct ttatttggt 
      tgtaaataa aagtatttt gttgttttt tgttaagtg 
      tgccctttg tggggcgtc aagcgaagc accctcgca 
      cagatttga actgattga aaaaacaaa ggaaattaa 
      tttacttaa ccacttgga taagcaaat taccgctga 
      agaatcttt gtcactatt gtgtacttt tgaataaat 
      tcgaaaaac aaacggtca atgccttca aatttttaa 
      atatgtgta ccatgtatt ctcgataat ctgtcttta 
      atagaaaag aaaaattct tgcaaaaat catggaaaa 
      aatattttt tatttacga ggcgatgcc gagtatgga 
      ggcacacat tgaataagt tactttatc ccagtgttg 
      tcttgcctt tattcagta ataataata ataaaacaa 
      aaatgataa caagtagaa atttttttc ttcgctcca 
      tattgtttt atttttgaa aaagttaat ttttctagc 
      catcgtttc aaatgattt agggttgtt ttaaaaaaa 
      attagttgt ctacaattt tggccctta tgacattca 
      taataatga atttttgaa tgtgtttgt tgcccctga 
      aaaaatatg aaacgaatt ttattatac agggtacct 
      aactcacat aatatgaca agcatacaa ggtgtgtcg 
      cctaaaagt ttagtttta ctatacttt tcttatctc 
      ggtctaaaa gtaacaaaa tttaaagtt tttttgtgc 
      aaaaaattc aaaaaaaaa ttggcgcca accccaaaa 
      aattattaa ataaagatt taaccaacg tattttttg 
      ggctagaga taatttttt atgtttttt ttgcacaag 
      atattaagc atcgtttga ttcaaaaat tctgaaaaa 
      aactttcgg cagaacgct tgaattatt taaaaaata 
      cattaatca attaggtga gatgtaaag cttgacttt 
      ttacaagaa ttacctgta gtttttggt aaaatattt 
      gctataatt tttatttct tattctgaa gtattattt 
      tattattcc tctgtctgt aatttgtct gtttatttt 
      tcttcgccc tttttcaaa attttgttg ctcccaaac 
      tataataat tgaatgaca ttttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY