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Gene information
General information
TC003898 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid25: 921844..923271
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003898
    iBeetle screen iB_03647
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003898 RA
      MNPLCAYLG YLFVNYCLN DKVQNEPIK PIKNQTFQL 
      EYQWHYVNY TWPTYDDYK KAVIKRKYV PENVAITGI 
      KYHKGDLYL AMPQIRKGV PVTLGKIST ESAPKSNPL 
      VHPYPSWEH QPRSKDCTT LQSVQNMEI DKSGVMWVL 
      DGFRINNNT RCPPKLVLF DLNNDGKEL LSYIFPKDI 
      CSSQGGHLK DIVIDSTND GFAYITDNS FLDPGLIVY 
      SRGQNRAWK LRDRTMFDE EDASGFVVG NLTFKGLAP 
      LDGIALSPI HPKTRNRLL FYSPLAGMN LYAIKSKVL 
      KSERLINSG DWRRAIKVV GKKQSQSDG LIMDNKGNL 
      YYTLPPLYG VGKWNMHEE FSTAKIMAE DKNMVWTGS 
      FAFDEFGNL RLLTNNINK YMDAGFPLK LSQEVMFKI 
      FKYFTGTKS YLES
    CDS sequence
    • >TC003898 RA
      atgaaccct ctgtgcgcc tatctcggg taccttttc 
      gtcaactac tgtctcaat gacaaggtt caaaacgaa 
      cccatcaag cccatcaag aaccaaacg ttccaactg 
      gagtaccag tggcactac gtcaactac acgtggccc 
      acctacgac gactacaaa aaggcggtc atcaagcgg 
      aagtacgtc ccggaaaat gtcgcaatc accggaatc 
      aagtaccac aaaggggac ctttacctc gcgatgccc 
      caaatccgg aagggcgtt ccggtaacc ctgggtaaa 
      atctccacc gaaagcgcc cccaaaagc aaccctctg 
      gtgcacccc taccccagc tgggagcac cagccccgg 
      agcaaagac tgcacgact ttacaaagc gtccagaac 
      atggaaatc gacaaaagc ggggtcatg tgggttctg 
      gacggcttc cggatcaac aacaacacc agatgcccc 
      ccaaaacta gtcctcttc gacctaaac aacgacgga 
      aaagagctc ctctcgtac attttcccc aaggatatt 
      tgctcgagt caagggggg cacctgaag gacattgtg 
      atcgatagc acaaacgac ggcttcgcc tacatcacg 
      gacaacagt ttcctggac ccgggcctg atcgtctac 
      tccaggggc caaaaccgg gcctggaaa ctgcgcgac 
      cgcacgatg ttcgacgaa gaggacgcg tccggtttt 
      gtcgtgggg aatctgacg ttcaagggg ctggcgccg 
      ctggacggg attgccctc tctcccata caccccaag 
      acccgcaat cgcctcttg ttctactct cctctagcc 
      gggatgaac ctctacgcc atcaagagc aaagtcttg 
      aagagcgaa cggttgatt aatagtggc gattggagg 
      agggcgatc aaggttgtg gggaagaaa caaagtcag 
      agcgatggc ttgatcatg gacaacaag gggaatctc 
      tactatacg ctgccgccc ttgtatggg gttgggaag 
      tggaacatg cacgaggag ttttccacg gcgaaaatc 
      atggccgag gataagaat atggtctgg acggggagt 
      ttcgccttt gacgagttt gggaatttg agactgttg 
      accaacaat ataaataag tatatggac gcgggtttc 
      ccgctgaaa ttgagccag gaagtcatg ttcaaaatt 
      ttcaagtac ttcacgggc acgaagagc tatttagaa 
      tcctaa
    mRNA sequence
    • >TC003898 RA
      cccacgtta taaatatat gtcaaagca aaaacggcc 
      agtttagtg caatcgcca gcgttgcaa tgaaccctc 
      tgtgcgcct atctcgggt accttttcg tcaactact 
      gtctcaatg acaaggttc aaaacgaac ccatcaagc 
      ccatcaaga accaaacgt tccaactgg agtaccagt 
      ggcactacg tcaactaca cgtggccca cctacgacg 
      actacaaaa aggcggtca tcaagcgga agtacgtcc 
      cggaaaatg tcgcaatca ccggaatca agtaccaca 
      aaggggacc tttacctcg cgatgcccc aaatccgga 
      agggcgttc cggtaaccc tgggtaaaa tctccaccg 
      aaagcgccc ccaaaagca accctctgg tgcacccct 
      accccagct gggagcacc agccccgga gcaaagact 
      gcacgactt tacaaagcg tccagaaca tggaaatcg 
      acaaaagcg gggtcatgt gggttctgg acggcttcc 
      ggatcaaca acaacacca gatgccccc caaaactag 
      tcctcttcg acctaaaca acgacggaa aagagctcc 
      tctcgtaca ttttcccca aggatattt gctcgagtc 
      aaggggggc acctgaagg acattgtga tcgatagca 
      caaacgacg gcttcgcct acatcacgg acaacagtt 
      tcctggacc cgggcctga tcgtctact ccaggggcc 
      aaaaccggg cctggaaac tgcgcgacc gcacgatgt 
      tcgacgaag aggacgcgt ccggttttg tcgtgggga 
      atctgacgt tcaaggggc tggcgccgc tggacggga 
      ttgccctct ctcccatac accccaaga cccgcaatc 
      gcctcttgt tctactctc ctctagccg ggatgaacc 
      tctacgcca tcaagagca aagtcttga agagcgaac 
      ggttgatta atagtggcg attggagga gggcgatca 
      aggttgtgg ggaagaaac aaagtcaga gcgatggct 
      tgatcatgg acaacaagg ggaatctct actatacgc 
      tgccgccct tgtatgggg ttgggaagt ggaacatgc 
      acgaggagt tttccacgg cgaaaatca tggccgagg 
      ataagaata tggtctgga cggggagtt tcgcctttg 
      acgagtttg ggaatttga gactgttga ccaacaata 
      taaataagt atatggacg cgggtttcc cgctgaaat 
      tgagccagg aagtcatgt tcaaaattt tcaagtact 
      tcacgggca cgaagagct atttagaat cctaagtta 
      tttattgtg atttttgtt gatttgttg ttattaggt 
      ttagagtac acaaagagt aggtaggtt gagacactt 
      tcgagtcat tttttacac acttatgta tatttgatt 
      gccaaatat aattttatt ttcata