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Gene information
General information
TC003874 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 25506677..25521460
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003874
    iBeetle screen iB_03643

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    remarks: later than schedule, hours 48 remarks: later than schedule, hours 48 remarks: later than schedule, hours 48
    Pupal morphology
    remarks: later than schedule, hours 48
    Adult morphology
    remarks: later than schedule, hours 48 remarks: later than schedule, hours 48
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 remarks: later than schedule, hours 48 (egglay)
    remarks: later than schedule, hours 48
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    remarks: later than schedule, hours 48
    remarks - cuticles: later than schedule, hours 48
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003874 RA
      MSEAKVRDN EALPKGRVT EVCQEWMVR EDGALAYRL 
      QNQEITEHL SGNKYRNAL VREDFPRAK SEQLREQQL 
      AEQTALIYQ RMLAEQEEQ DNQVAKQLA EELEREERQ 
      KRRFIEIQD QNVARQLLH RERVSVERH ALSPQRPPP 
      PRNYDQNLS PHRPNSANY QANLPRRQA KPMPLPSDL 
      YTEPYRTPE ALTSQLERI DIAEVGLPL DELTERQIQ 
      EERDAELAR KLQEQEGSL EDTLLNRDR MLAIEAQDK 
      ELAKMLQER ERSKARRAR ERAKQKALA KKQLESQQS 
      TANQIMPDD AYSYPADMI PPRASVPRN HPIISDTYA 
      LPNQNEDDI NYSLPADVL PSHNLIDNN VKSNYSPQK 
      SYDSRYALG DKVCNSTDS SIRPSFDTD RNLDGPPAV 
      RPTQLDLKS PLNRINKPR YPEPEGCDN ASGLSPNSQ 
      HINIAMAID PTYPKRGMH SSSSYETTS STVTTSTSS 
      SSPGMVLPP PDISELDDE NNIPPYMPI QGQRRTASL 
      EKKQKKKSK DGGCKQQ
    CDS sequence
    • >TC003874 RA
      atgtctgaa gcaaaagtt cgcgacaac gaggcactg 
      ccaaagggc cgagtcacc gaagtgtgc caggaatgg 
      atggtgcgt gaagatggg gccctggca taccgcctc 
      cagaaccag gagatcacc gaacacctc tcaggtaac 
      aaatatcgc aatgcccta gtccgcgaa gacttccct 
      cgggccaaa agcgagcaa ctcagagaa cagcagctg 
      gccgaacag acggcccta atttaccag cggatgttg 
      gccgaacaa gaggagcaa gacaatcaa gtggctaag 
      cagctcgca gaggagctg gagcgagaa gaacgccaa 
      aaacgtaga tttatcgaa attcaggac caaaacgtt 
      gcccgacag cttttgcac agggagcga gtcagtgtt 
      gaaagacac gcactttcg ccccagagg cctccgccc 
      cctaggaac tacgaccag aatttgtcg ccccatcgg 
      cccaattcc gccaattat caggcgaac ttgccgaga 
      agacaggcc aagcctatg cctctgcct tcggatttg 
      tacacggag ccctatcgc acacctgag gctttaacc 
      tcacagttg gagcgaatc gacatcgct gaagtgggg 
      ctccctcta gacgaacta accgaacgt caaattcaa 
      gaagagcgc gacgctgaa ttggcccga aaattgcag 
      gaacaagaa ggcagtttg gaagacact ttgctaaat 
      cgcgatcga atgctcgca atcgaagcg caggacaaa 
      gaactggca aagatgttg caggaaagg gagaggagc 
      aaagcccga agggcgcgg gaaagggct aaacagaaa 
      gcccttgct aaaaaacag ctcgaatct caacagtcc 
      actgccaat caaatcatg ccagacgat gcgtattcg 
      tatccagct gacatgatc ccgcctaga gcctcagtg 
      ccaagaaac catccgatc atatcagat acatatgca 
      ctaccaaat cagaacgaa gatgatatt aattacagt 
      ttacctgct gatgttcta ccatcacat aatttaatc 
      gacaataat gttaaatca aattatagt ccgcagaaa 
      agctacgat tcacgttat gccttaggt gataaagtt 
      tgtaatagt actgactca agtattcgt ccgtccttt 
      gatactgat aggaattta gatgggcct ccagccgta 
      cgaccgact caattggac ttgaagtcg cccttgaac 
      cgaatcaat aaacccaga tatccggaa ccagaaggc 
      tgtgataat gcaagtggg ctgtcgccc aattcgcaa 
      catatcaac atagccatg gctatcgac cctacatat 
      cccaaaaga ggaatgcat tcctcgtcc agctatgaa 
      actacttca agtactgta actacaagt acttccagt 
      agtagccct ggtatggtt ttaccgccc cctgatatc 
      tcagaacta gatgatgaa aacaatatt ccaccctat 
      atgccaatc caaggccaa cgcagaacc gcctctttg 
      gagaaaaaa caaaagaaa aagagcaaa gatggtggc 
      tgtaaacag caatag
    mRNA sequence
    • >TC003874 RA
      tgtgttttg ggaaatgtg cccaacatt ttgacattt 
      ctgacagat aatcaacac attttataa cttgacaaa 
      cttcaaact gttctttgt tgttagaac atgaataat 
      gtaattatt ttacttatt tgtgtcgca ttatacgat 
      aaaatgtca tctctagtt agtaaatcc ccgattcac 
      taaccccaa aaatagtaa aataatatg agtaagagg 
      taattgtct tgtgctcag tgacccgta tgtttttgt 
      gtcaacacc agtgaatga gtcagttaa atatttact 
      tccgaccat gtctgaagc aaaagttcg cgacaacga 
      ggcactgcc aaagggccg agtcaccga agtgtgcca 
      ggaatggat ggtgcgtga agatggggc cctggcata 
      ccgcctcca gaaccagga gatcaccga acacctctc 
      aggtaacaa atatcgcaa tgccctagt ccgcgaaga 
      cttccctcg ggccaaaag cgagcaact cagagaaca 
      gcagctggc cgaacagac ggccctaat ttaccagcg 
      gatgttggc cgaacaaga ggagcaaga caatcaagt 
      ggctaagca gctcgcaga ggagctgga gcgagaaga 
      acgccaaaa acgtagatt tatcgaaat tcaggacca 
      aaacgttgc ccgacagct tttgcacag ggagcgagt 
      cagtgttga aagacacgc actttcgcc ccagaggcc 
      tccgccccc taggaacta cgaccagaa tttgtcgcc 
      ccatcggcc caattccgc caattatca ggcgaactt 
      gccgagaag acaggccaa gcctatgcc tctgccttc 
      ggatttgta cacggagcc ctatcgcac acctgaggc 
      tttaacctc acagttgga gcgaatcga catcgctga 
      agtggggct ccctctaga cgaactaac cgaacgtca 
      aattcaaga agagcgcga cgctgaatt ggcccgaaa 
      attgcagga acaagaagg cagtttgga agacacttt 
      gctaaatcg cgatcgaat gctcgcaat cgaagcgca 
      ggacaaaga actggcaaa gatgttgca ggaaaggga 
      gaggagcaa agcccgaag ggcgcggga aagggctaa 
      acagaaagc ccttgctaa aaaacagct cgaatctca 
      acagtccac tgccaatca aatcatgcc agacgatgc 
      gtattcgta tccagctga catgatccc gcctagagc 
      ctcagtgcc aagaaacca tccgatcat atcagatac 
      atatgcact accaaatca gaacgaaga tgatattaa 
      ttacagttt acctgctga tgttctacc atcacataa 
      tttaatcga caataatgt taaatcaaa ttatagtcc 
      gcagaaaag ctacgattc acgttatgc cttaggtga 
      taaagtttg taatagtac tgactcaag tattcgtcc 
      gtcctttga tactgatag gaatttaga tgggcctcc 
      agccgtacg accgactca attggactt gaagtcgcc 
      cttgaaccg aatcaataa acccagata tccggaacc 
      agaaggctg tgataatgc aagtgggct gtcgcccaa 
      ttcgcaaca tatcaacat agccatggc tatcgaccc 
      tacatatcc caaaagagg aatgcattc ctcgtccag 
      ctatgaaac tacttcaag tactgtaac tacaagtac 
      ttccagtag tagccctgg tatggtttt accgccccc 
      tgatatctc agaactaga tgatgaaaa caatattcc 
      accctatat gccaatcca aggccaacg cagaaccgc 
      ctctttgga gaaaaaaca aaagaaaaa gagcaaaga 
      tggtggctg taaacagca ataggatat tttttattt 
      taattcagt ttgttgtta ttgtgaaac gtttgatat 
      tttcgtaag cagctccaa attgacaat aggttaata 
      tctatttat atttttatt atttatagt tacataata 
      ccaaaatat tcatgctca acgtatatg ttaagtaat 
      atatttaca ctttatact tagtattaa ttaatttgg 
      taacaggtt ttacttttt tgtactttt tacaaaata 
      aagaatttt caatgttaa ttattgctt tatttacaa 
      tttcacaag atcaacctt actttttat aatgtgtac 
      aaaaggtaa tacgaaatt agagcaatt gcatttctg 
      gccagctac aaaattaaa tgccccaat tcgtgaatc 
      acactgtgg acaatttat gcgcccttt agaccagtt 
      ctcttccgc tatcctgtc tttaatcca ctgggggac 
      gtaatcctc attcaaaaa aacaacgtt ctccacatt 
      acattcaga gtcatttaa attgtccaa gacaacact 
      gtgggcact caaaaataa tgattcaca ttcgttatc 
      gccaaatat tcggcttcg acatcgttt acacctcac 
      ttcggaata catttgagt gcaaaaaaa tattttaat 
      taataagcc ataacctta aaatggcat gtgtctggt 
      ctgttgcca accgcaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY