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Gene information
General information
TC003873 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 25498877..25500706
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003873
    iBeetle screen iB_03642

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003873 RA
      MNSKHLTKG SRKPSPVRR GKLRLYSNR 
      FCPYCQRVI LVLDAKRIP YEVVNINLV 
      NKPDWMFDK SPMGKVPAL ELDNGEVLY 
      ESLIIADYL DDKYNAHRL HAKDPLQKA 
      KDQLLLKQF TKIINALLK TVSLGRIEH 
      DEAQVISEG LAIFERELT DRPGPFFGG 
      SRPGMLDYM IWPWCERSD VLKIYGKDY 
      ILKKEKYKQ LMEWRKVMV EDEAVKKSW 
      CSANTHIKY LQSSRAGVP NYDLLI
    CDS sequence
    • >TC003873 RA
      atgaattcc aaacatctt accaaagga tcaagaaaa 
      ccatctcct gttcgaagg ggcaaactc cggttatac 
      agtaatcga ttttgtcct tattgccaa cgtgttatc 
      ctagtcctg gacgcaaaa aggatacca tacgaagtg 
      gttaacata aatctggtc aataaaccc gactggatg 
      ttcgataag tcaccaatg ggtaaagta ccggccctt 
      gaacttgat aacggtgaa gtcctctat gaatcttta 
      attatcgct gattattta gacgataaa tacaacgcc 
      caccggtta catgcaaag gaccctttg caaaaagcg 
      aaagaccaa cttttactg aagcaattc accaagatt 
      attaacgct ttactgaaa acggtctcc ttgggtcga 
      attgaacac gacgaagca caagtcatt tccgaagga 
      cttgcaata tttgaaagg gaattaact gacagacct 
      gggccattt tttggtggt agtaggccg ggaatgttg 
      gactacatg atatggcca tggtgtgaa cgttccgat 
      gtgctgaag atttatggc aaggactac attctcaaa 
      aaagaaaaa tacaaacaa cttatggaa tggcgtaag 
      gttatggtg gaagatgag gctgtgaaa aagagttgg 
      tgtagtgct aacacccac ataaaatat ttgcagtca 
      tccagggct ggagtaccc aactatgac ttactcata 
      taa
    mRNA sequence
    • >TC003873 RA
      tttattaaa taacacaat aaaaccaat taattgaaa 
      taacaaagt gcgaaaaaa ctataatga attccaaac 
      atcttacca aaggatcaa gaaaaccat ctcctgttc 
      gaaggggca aactccggt tatacagta atcgatttt 
      gtccttatt gccaacgtg ttatcctag tcctggacg 
      caaaaagga taccatacg aagtggtta acataaatc 
      tggtcaata aacccgact ggatgttcg ataagtcac 
      caatgggta aagtaccgg cccttgaac ttgataacg 
      gtgaagtcc tctatgaat ctttaatta tcgctgatt 
      atttagacg ataaataca acgcccacc ggttacatg 
      caaaggacc ctttgcaaa aagcgaaag accaacttt 
      tactgaagc aattcacca agattatta acgctttac 
      tgaaaacgg tctccttgg gtcgaattg aacacgacg 
      aagcacaag tcatttccg aaggacttg caatatttg 
      aaagggaat taactgaca gacctgggc cattttttg 
      gtggtagta ggccgggaa tgttggact acatgatat 
      ggccatggt gtgaacgtt ccgatgtgc tgaagattt 
      atggcaagg actacattc tcaaaaaag aaaaataca 
      aacaactta tggaatggc gtaaggtta tggtggaag 
      atgaggctg tgaaaaaga gttggtgta gtgctaaca 
      cccacataa aatatttgc agtcatcca gggctggag 
      tacccaact atgacttac tcatataag tagtaaaaa 
      agccccttt agaacactt tgtaacatt attatataa 
      acattataa tatataaaa tattatctc ttgttttac 
      ataaaatcg aattcgaaa taaataagt ctatttcgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY