Status
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Gene information
General information
TC031061
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003865,TC003867,TC003866
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 23733801..23748555
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031061
    iBeetle screen iB_03640

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031061 RA
      MSGTHLIMP NPWSDKNTA LVEKQLVYK DQEEFANQL 
      MKIAGNELR EDEKTRKQC LEQLREWIR KNQDIENCR 
      TDDRFLLRF LRVKKYSIH MAEQTLLKY LNFRKRFKE 
      FMYGLDHLK SPLISNGYI FVSPFRDAN GRRVIIYNV 
      SKVDPHQFT GTDVGLAHA ITYETLLAD EDNQIAGVN 
      HVCDADGLS AAFLTLWSI TEFATIIRW GEQSLPMRH 
      KEINLLNFP SALKYVYDF VHSTIKSEK LKNRIMIHG 
      SLTDLHNKV DKKCLPLEL GGVMPAKEM AELWKKELA 
      AKRELLLSY DAMNLLSDR GIICRRNLP NEDNTGVGS 
      LPGSFRKLE VD
    • >TC031061 RB
      MSGTHLIMP NPWSDKNTA LVEKQLVYK DQEEFANQL 
      MKIAGNELR EDEKTRKQC LEQLREWIR KNQDIENCR 
      TDDRFLLRF LRVKKYSIH MAEQTLLKY LNFRKRFKE 
      FMYGLDHLK SPLISNGYI FVSPFRDAN GRRVIIYNV 
      SKVDPHQFT GTDVGLAHA ITYETLLAD EDNQIAGVN 
      HVCDADGLS AAFLTLWSI TEFATIIRW GEQSLPMRH 
      KEINLLNFP SALKYVYDF VHSTIKSEK LKNRIMIHG 
      SLTDLHNKV DKKCLPLEL GGVMPAKEM AELWKKELA 
      AKRELLLSY DAMNLLSDR GIICRRNLP NEDNTGFHG 
      SLTELHNKV DKRCLPLEL GGVMPAKEM AELWKKELA 
      AKRELLLSY DAMNLLSDR GIICRRNLP NEDNTGVGS 
      LPGSFRKLE VD
    • >TC031061 RC
      MSGTHLIMP NPWSDKNTA LVEKQLVYK DQEEFANQL 
      MKIAGNELR EDEKTRKQC LEQLREWIR KNQDIENCR 
      TDDRFLLRF LRVKKYSIH MAEQTLLKY LNFRKRFKE 
      FMYGLDHLK SPLISNGYI FVSPFRDAN GRRVIIYNV 
      SKVDPHQFT GTDVGLAHA ITYETLLAD EDNQIAGVN 
      HVCDADGLS AAFLTLWSI TEFATIIRW GEQSLPMRH 
      KEINLLNFP SALKYVYDF VHSTIKSEK LKNRIMIHG 
      SLTDLHNKV DKKCLPLEL GGVMPAKEM AELWKKELA 
      AKRELLLSY DAMNLLSDR GIICRRNLP NEDNTGIHG 
      SLTELHNKV DKRCLPLEL GGVMPAKEM AELWKKELA 
      AKRELLLSY DAMNLLSDR GIICRRNLP NEDNTGFHG 
      SLTELHNKV DKRCLPLEL GGVMPAKEM AELWKKELA 
      AKRELLLSY DAMNLLSDR GIICRRNLP NEDNTGVGS 
      LPGSFRKLE VD
    CDS sequence
    • >TC031061 RA
      atgtctgga acacaccta ataatgcca aacccttgg 
      agcgataaa aacacggct cttgttgag aaacaatta 
      gtctacaag gatcaagaa gagttcgct aatcaactt 
      atgaaaatt gcggggaac gaactcaga gaggatgaa 
      aaaacacgg aaacaatgc ttggaacag ctcagggag 
      tggataagg aaaaatcag gacattgaa aactgccgt 
      actgatgac agatttctt ctccgtttc ttacgagtg 
      aagaagtac agtatacac atggctgag caaaccctt 
      ttgaagtac ttaaacttc aggaaaagg tttaaagag 
      tttatgtac ggtctggat catcttaaa agccccctc 
      attagcaat ggttatatt tttgtgagc ccgttccgg 
      gatgctaat gggaggaga gtaattatt tataatgta 
      agtaaagtc gatccgcat caatttacc ggaacggat 
      gttggctta gctcatgcc attacttac gagactctt 
      ttggcggat gaagacaac cagattgct ggggttaac 
      catgtttgt gatgccgat ggcctaagt gctgcgttt 
      ctcactctg tggtccatc acagagttt gcaacgatt 
      attcgttgg ggggagcaa tcactgccg atgaggcac 
      aaggaaatc aatctttta aacttcccg tcagccctt 
      aagtacgtc tacgacttt gttcactcc acaattaag 
      agtgaaaaa ttgaaaaac aggattatg attcatggc 
      tcgttaacc gacctgcat aataaagtg gataaaaag 
      tgtctaccc ttggaattg ggcggtgtt atgccggcg 
      aaagaaatg gcagaattg tggaagaaa gaattagct 
      gcgaaaaga gagcttctc ttatcttac gacgcaatg 
      aatttgttg agtgacaga ggtataata tgcaggcga 
      aatttacct aatgaggat aataccggc gttgggagt 
      cttcccgga agttttcga aaacttgaa gttgattaa
    • >TC031061 RB
      atgtctgga acacaccta ataatgcca aacccttgg 
      agcgataaa aacacggct cttgttgag aaacaatta 
      gtctacaag gatcaagaa gagttcgct aatcaactt 
      atgaaaatt gcggggaac gaactcaga gaggatgaa 
      aaaacacgg aaacaatgc ttggaacag ctcagggag 
      tggataagg aaaaatcag gacattgaa aactgccgt 
      actgatgac agatttctt ctccgtttc ttacgagtg 
      aagaagtac agtatacac atggctgag caaaccctt 
      ttgaagtac ttaaacttc aggaaaagg tttaaagag 
      tttatgtac ggtctggat catcttaaa agccccctc 
      attagcaat ggttatatt tttgtgagc ccgttccgg 
      gatgctaat gggaggaga gtaattatt tataatgta 
      agtaaagtc gatccgcat caatttacc ggaacggat 
      gttggctta gctcatgcc attacttac gagactctt 
      ttggcggat gaagacaac cagattgct ggggttaac 
      catgtttgt gatgccgat ggcctaagt gctgcgttt 
      ctcactctg tggtccatc acagagttt gcaacgatt 
      attcgttgg ggggagcaa tcactgccg atgaggcac 
      aaggaaatc aatctttta aacttcccg tcagccctt 
      aagtacgtc tacgacttt gttcactcc acaattaag 
      agtgaaaaa ttgaaaaac aggattatg attcatggc 
      tcgttaacc gacctgcat aataaagtg gataaaaag 
      tgtctaccc ttggaattg ggcggtgtt atgccggcg 
      aaagaaatg gcagaattg tggaagaaa gaattagct 
      gcgaaaaga gagcttctc ttatcttac gacgcaatg 
      aatttgttg agtgacaga ggtataata tgcaggcga 
      aatttacct aatgaggat aataccggc tttcatggc 
      tctttaacc gagctgcat aataaagtg gataaaagg 
      tgtctaccc ttggaattg ggcggtgtt atgccggcg 
      aaagaaatg gcagaattg tggaagaaa gaattagct 
      gcgaaaaga gagcttctc ttatcttac gacgcaatg 
      aatttgttg agtgacaga ggtataata tgcaggcga 
      aatttacct aatgaggat aataccggc gttgggagt 
      cttcccgga agttttcga aaacttgaa gttgattaa
    • >TC031061 RC
      atgtctgga acacaccta ataatgcca aacccttgg 
      agcgataaa aacacggct cttgttgag aaacaatta 
      gtctacaag gatcaagaa gagttcgct aatcaactt 
      atgaaaatt gcggggaac gaactcaga gaggatgaa 
      aaaacacgg aaacaatgc ttggaacag ctcagggag 
      tggataagg aaaaatcag gacattgaa aactgccgt 
      actgatgac agatttctt ctccgtttc ttacgagtg 
      aagaagtac agtatacac atggctgag caaaccctt 
      ttgaagtac ttaaacttc aggaaaagg tttaaagag 
      tttatgtac ggtctggat catcttaaa agccccctc 
      attagcaat ggttatatt tttgtgagc ccgttccgg 
      gatgctaat gggaggaga gtaattatt tataatgta 
      agtaaagtc gatccgcat caatttacc ggaacggat 
      gttggctta gctcatgcc attacttac gagactctt 
      ttggcggat gaagacaac cagattgct ggggttaac 
      catgtttgt gatgccgat ggcctaagt gctgcgttt 
      ctcactctg tggtccatc acagagttt gcaacgatt 
      attcgttgg ggggagcaa tcactgccg atgaggcac 
      aaggaaatc aatctttta aacttcccg tcagccctt 
      aagtacgtc tacgacttt gttcactcc acaattaag 
      agtgaaaaa ttgaaaaac aggattatg attcatggc 
      tcgttaacc gacctgcat aataaagtg gataaaaag 
      tgtctaccc ttggaattg ggcggtgtt atgccggcg 
      aaagaaatg gcagaattg tggaagaaa gaattagct 
      gcgaaaaga gagcttctc ttatcttac gacgcaatg 
      aatttgttg agtgacaga ggtataata tgcaggcga 
      aatttacct aatgaggat aataccggc attcatggc 
      tcgttaacc gagctgcat aataaagtg gataaaagg 
      tgtctaccc ttggaattg ggcggtgtt atgccggcg 
      aaagaaatg gcagaattg tggaagaaa gaattagct 
      gcgaaaaga gagcttctc ttatcttac gacgcaatg 
      aatttgttg agtgacaga ggtataata tgcaggcga 
      aatttacct aatgaggat aataccggc tttcatggc 
      tctttaacc gagctgcat aataaagtg gataaaagg 
      tgtctaccc ttggaattg ggcggtgtt atgccggcg 
      aaagaaatg gcagaattg tggaagaaa gaattagct 
      gcgaaaaga gagcttctc ttatcttac gacgcaatg 
      aatttgttg agtgacaga ggtataata tgcaggcga 
      aatttacct aatgaggat aataccggc gttgggagt 
      cttcccgga agttttcga aaacttgaa gttgattaa
    mRNA sequence
    • >TC031061 RA
      ttatttcaa attcagttt tattcgtag ttgattgca 
      cagtaattg ttcattttg gccagcagt tgtgattcc 
      gaaacgatc gcttcacga gcacatcag ataaatttt 
      aggatgtct ggaacacac ctaataatg ccaaaccct 
      tggagcgat aaaaacacg gctcttgtt gagaaacaa 
      ttagtctac aaggatcaa gaagagttc gctaatcaa 
      cttatgaaa attgcgggg aacgaactc agagaggat 
      gaaaaaaca cggaaacaa tgcttggaa cagctcagg 
      gagtggata aggaaaaat caggacatt gaaaactgc 
      cgtactgat gacagattt cttctccgt ttcttacga 
      gtgaagaag tacagtata cacatggct gagcaaacc 
      cttttgaag tacttaaac ttcaggaaa aggtttaaa 
      gagtttatg tacggtctg gatcatctt aaaagcccc 
      ctcattagc aatggttat atttttgtg agcccgttc 
      cgggatgct aatgggagg agagtaatt atttataat 
      gtaagtaaa gtcgatccg catcaattt accggaacg 
      gatgttggc ttagctcat gccattact tacgagact 
      cttttggcg gatgaagac aaccagatt gctggggtt 
      aaccatgtt tgtgatgcc gatggccta agtgctgcg 
      tttctcact ctgtggtcc atcacagag tttgcaacg 
      attattcgt tggggggag caatcactg ccgatgagg 
      cacaaggaa atcaatctt ttaaacttc ccgtcagcc 
      cttaagtac gtctacgac tttgttcac tccacaatt 
      aagagtgaa aaattgaaa aacaggatt atgattcat 
      ggctcgtta accgacctg cataataaa gtggataaa 
      aagtgtcta cccttggaa ttgggcggt gttatgccg 
      gcgaaagaa atggcagaa ttgtggaag aaagaatta 
      gctgcgaaa agagagctt ctcttatct tacgacgca 
      atgaatttg ttgagtgac agaggtata atatgcagg 
      cgaaattta cctaatgag gataatacc ggcgttggg 
      agtcttccc ggaagtttt cgaaaactt gaagttgat 
      taagtttgt ttaagttta actttattt tttgtttgt 
      tttagagta gcagtagtt tgttaattt
    • >TC031061 RB
      ttatttcaa attcagttt tattcgtag ttgattgca 
      cagtaattg ttcattttg gccagcagt tgtgattcc 
      gaaacgatc gcttcacga gcacatcag ataaatttt 
      aggatgtct ggaacacac ctaataatg ccaaaccct 
      tggagcgat aaaaacacg gctcttgtt gagaaacaa 
      ttagtctac aaggatcaa gaagagttc gctaatcaa 
      cttatgaaa attgcgggg aacgaactc agagaggat 
      gaaaaaaca cggaaacaa tgcttggaa cagctcagg 
      gagtggata aggaaaaat caggacatt gaaaactgc 
      cgtactgat gacagattt cttctccgt ttcttacga 
      gtgaagaag tacagtata cacatggct gagcaaacc 
      cttttgaag tacttaaac ttcaggaaa aggtttaaa 
      gagtttatg tacggtctg gatcatctt aaaagcccc 
      ctcattagc aatggttat atttttgtg agcccgttc 
      cgggatgct aatgggagg agagtaatt atttataat 
      gtaagtaaa gtcgatccg catcaattt accggaacg 
      gatgttggc ttagctcat gccattact tacgagact 
      cttttggcg gatgaagac aaccagatt gctggggtt 
      aaccatgtt tgtgatgcc gatggccta agtgctgcg 
      tttctcact ctgtggtcc atcacagag tttgcaacg 
      attattcgt tggggggag caatcactg ccgatgagg 
      cacaaggaa atcaatctt ttaaacttc ccgtcagcc 
      cttaagtac gtctacgac tttgttcac tccacaatt 
      aagagtgaa aaattgaaa aacaggatt atgattcat 
      ggctcgtta accgacctg cataataaa gtggataaa 
      aagtgtcta cccttggaa ttgggcggt gttatgccg 
      gcgaaagaa atggcagaa ttgtggaag aaagaatta 
      gctgcgaaa agagagctt ctcttatct tacgacgca 
      atgaatttg ttgagtgac agaggtata atatgcagg 
      cgaaattta cctaatgag gataatacc ggctttcat 
      ggctcttta accgagctg cataataaa gtggataaa 
      aggtgtcta cccttggaa ttgggcggt gttatgccg 
      gcgaaagaa atggcagaa ttgtggaag aaagaatta 
      gctgcgaaa agagagctt ctcttatct tacgacgca 
      atgaatttg ttgagtgac agaggtata atatgcagg 
      cgaaattta cctaatgag gataatacc ggcgttggg 
      agtcttccc ggaagtttt cgaaaactt gaagttgat 
      taagtttgt ttaagttta actttattt tttgtttgt 
      tttagagta gcagtagtt tgttaattt
    • >TC031061 RC
      ttatttcaa attcagttt tattcgtag ttgattgca 
      cagtaattg ttcattttg gccagcagt tgtgattcc 
      gaaacgatc gcttcacga gcacatcag ataaatttt 
      aggatgtct ggaacacac ctaataatg ccaaaccct 
      tggagcgat aaaaacacg gctcttgtt gagaaacaa 
      ttagtctac aaggatcaa gaagagttc gctaatcaa 
      cttatgaaa attgcgggg aacgaactc agagaggat 
      gaaaaaaca cggaaacaa tgcttggaa cagctcagg 
      gagtggata aggaaaaat caggacatt gaaaactgc 
      cgtactgat gacagattt cttctccgt ttcttacga 
      gtgaagaag tacagtata cacatggct gagcaaacc 
      cttttgaag tacttaaac ttcaggaaa aggtttaaa 
      gagtttatg tacggtctg gatcatctt aaaagcccc 
      ctcattagc aatggttat atttttgtg agcccgttc 
      cgggatgct aatgggagg agagtaatt atttataat 
      gtaagtaaa gtcgatccg catcaattt accggaacg 
      gatgttggc ttagctcat gccattact tacgagact 
      cttttggcg gatgaagac aaccagatt gctggggtt 
      aaccatgtt tgtgatgcc gatggccta agtgctgcg 
      tttctcact ctgtggtcc atcacagag tttgcaacg 
      attattcgt tggggggag caatcactg ccgatgagg 
      cacaaggaa atcaatctt ttaaacttc ccgtcagcc 
      cttaagtac gtctacgac tttgttcac tccacaatt 
      aagagtgaa aaattgaaa aacaggatt atgattcat 
      ggctcgtta accgacctg cataataaa gtggataaa 
      aagtgtcta cccttggaa ttgggcggt gttatgccg 
      gcgaaagaa atggcagaa ttgtggaag aaagaatta 
      gctgcgaaa agagagctt ctcttatct tacgacgca 
      atgaatttg ttgagtgac agaggtata atatgcagg 
      cgaaattta cctaatgag gataatacc ggcattcat 
      ggctcgtta accgagctg cataataaa gtggataaa 
      aggtgtcta cccttggaa ttgggcggt gttatgccg 
      gcgaaagaa atggcagaa ttgtggaag aaagaatta 
      gctgcgaaa agagagctt ctcttatct tacgacgca 
      atgaatttg ttgagtgac agaggtata atatgcagg 
      cgaaattta cctaatgag gataatacc ggctttcat 
      ggctcttta accgagctg cataataaa gtggataaa 
      aggtgtcta cccttggaa ttgggcggt gttatgccg 
      gcgaaagaa atggcagaa ttgtggaag aaagaatta 
      gctgcgaaa agagagctt ctcttatct tacgacgca 
      atgaatttg ttgagtgac agaggtata atatgcagg 
      cgaaattta cctaatgag gataatacc ggcgttggg 
      agtcttccc ggaagtttt cgaaaactt gaagttgat 
      taagtttgt ttaagttta actttattt tttgtttgt 
      tttagagta gcagtagtt tgttaattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY