Status
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Gene information
General information
TC031060
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003861,TC003860,TC003862
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 23543666..23555380
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031060
    iBeetle screen iB_03639

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031060 RA
      MAGKRRRPS TTEAGATKP KRAKFTLDS VKLPETTRE 
      GVLLVTGAG DCGQLGLGP DVLEKSRPA LVQTSDEIV 
      DICAGGMHT VCLTKEGKI LSFGCNDEG ALGRITEGK 
      EDAESTPGE VELPGKVIQ ITAGDSHSA ALLDDGRVF 
      AWGTFRDSH GNMGLTLRG NEKLPYEII QEVHVIKIA 
      SGADHIVFL TNHGEVYTC GCPEQGQLG RTSERGSSR 
      NARSNMGKG QIAKLLQPT IISLKPSLK LHFDNIWAG 
      TYCTFAKVA GKSDIYVFG LNNYNQLGL KSLAPQYLP 
      ILSKEFSKY NWSMISSAE HHTIALTEN GKTYAIGRK 
      DYGRLGLGK DCEDASELT EIPALKDKT VVYVACGSS 
      TSFAVTDKG ELYGWGMGT NGQLGNGEE EDCFEPTYV 
      KSKQLTDRP VFKVSAGGQ HAVILATNS NNNKGDS
    CDS sequence
    • >TC031060 RA
      atggccggt aaacgacgc cgccctagc acaaccgaa 
      gccggagcc accaagccc aagcgcgcg aagttcact 
      ttagacagc gtgaaatta ccagagact actcgtgag 
      ggggttctg cttgttacg ggagccggt gactgcggc 
      caattgggt ttggggccc gatgtgttg gagaaaagt 
      aggcccgca ctagtgcaa acaagtgat gaaattgtc 
      gatatttgc gctgggggt atgcacacg gtttgcttg 
      accaaagaa ggcaaaatt ttgagtttc gggtgtaat 
      gacgagggg gcacttgga cgtatcacg gagggcaag 
      gaggatgcg gaatccact cctggcgag gttgagttg 
      cctgggaaa gttatacag attacggca ggggactca 
      cattcggct gcgttactg gacgatggg agggtgttt 
      gcatggggg acatttcgt gactcacat ggtaatatg 
      ggcctgact ttgcgagga aacgaaaag ttaccttac 
      gaaataatc caggaggta cacgttatc aaaatagcg 
      tcaggggcc gaccacatt gttttttta acaaaccat 
      ggggaagtt tacacttgt ggatgtcct gagcaagga 
      caattgggc cgaacgagc gagagaggt agcagtaga 
      aatgctaga agtaatatg ggcaaggga caaatagca 
      aaattgtta caacccaca atcatcagt ctaaagccc 
      agtttaaag cttcatttt gacaacatc tgggctggc 
      acttattgc acgtttgcc aaagtggcc ggcaaaagt 
      gatatttac gtcttcgga cttaacaat tataaccag 
      ttaggttta aaaagctta gcacctcag tatttacca 
      atactttcc aaggagttt tccaaatac aattggagc 
      atgatttcg tcagctgaa caccacact attgcgttg 
      actgaaaat ggaaaaact tacgccatt ggtaggaag 
      gattacggg cgtctgggg ctaggcaaa gattgtgag 
      gatgcatca gagttgact gaaatccca gctttaaag 
      gacaaaact gttgtttat gtggcttgt ggctcgtcc 
      acttctttc gcagtcact gacaaaggg gagctttat 
      ggctggggt atgggaaca aatggccag ttagggaat 
      ggtgaagaa gaggattgt ttcgaaccc acttacgtg 
      aaaagcaag cagctgaca gacaggcca gtttttaaa 
      gtcagtgcc ggaggacaa catgctgtt attttagca 
      acaaattcg aataataat aaaggtgac tcttaa
    mRNA sequence
    • >TC031060 RA
      attttagta aaaaattgt gcaagtaat ggccggtaa 
      acgacgccg ccctagcac aaccgaagc cggagccac 
      caagcccaa gcgcgcgaa gttcacttt agacagcgt 
      gaaattacc agagactac tcgtgaggg ggttctgct 
      tgttacggg agccggtga ctgcggcca attgggttt 
      ggggcccga tgtgttgga gaaaagtag gcccgcact 
      agtgcaaac aagtgatga aattgtcga tatttgcgc 
      tgggggtat gcacacggt ttgcttgac caaagaagg 
      caaaatttt gagtttcgg gtgtaatga cgagggggc 
      acttggacg tatcacgga gggcaagga ggatgcgga 
      atccactcc tggcgaggt tgagttgcc tgggaaagt 
      tatacagat tacggcagg ggactcaca ttcggctgc 
      gttactgga cgatgggag ggtgtttgc atgggggac 
      atttcgtga ctcacatgg taatatggg cctgacttt 
      gcgaggaaa cgaaaagtt accttacga aataatcca 
      ggaggtaca cgttatcaa aatagcgtc aggggccga 
      ccacattgt ttttttaac aaaccatgg ggaagttta 
      cacttgtgg atgtcctga gcaaggaca attgggccg 
      aacgagcga gagaggtag cagtagaaa tgctagaag 
      taatatggg caagggaca aatagcaaa attgttaca 
      acccacaat catcagtct aaagcccag tttaaagct 
      tcattttga caacatctg ggctggcac ttattgcac 
      gtttgccaa agtggccgg caaaagtga tatttacgt 
      cttcggact taacaatta taaccagtt aggtttaaa 
      aagcttagc acctcagta tttaccaat actttccaa 
      ggagttttc caaatacaa ttggagcat gatttcgtc 
      agctgaaca ccacactat tgcgttgac tgaaaatgg 
      aaaaactta cgccattgg taggaagga ttacgggcg 
      tctggggct aggcaaaga ttgtgagga tgcatcaga 
      gttgactga aatcccagc tttaaagga caaaactgt 
      tgtttatgt ggcttgtgg ctcgtccac ttctttcgc 
      agtcactga caaagggga gctttatgg ctggggtat 
      gggaacaaa tggccagtt agggaatgg tgaagaaga 
      ggattgttt cgaacccac ttacgtgaa aagcaagca 
      gctgacaga caggccagt ttttaaagt cagtgccgg 
      aggacaaca tgctgttat tttagcaac aaattcgaa 
      taataataa aggtgactc ttaacaatt gcttcgaag 
      tgagtattt gtcaattta gctgcaaca ataattttg 
      tgtatccag gtagagata cactcacat tttttattt 
      gattgaatg ttggagaat tgagtaccg tttttgtat 
      aatttgtat tgttcactg ccgttacgt taatgttct 
      tagtattcg tttggtcaa acaagaagt gaaaatatt 
      gttattgtt agtgcagca attatattt tga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY