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Gene information
General information
TC003857 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid23: 860255..864849
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003857
    iBeetle screen iB_03637
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands sometimes size increased - Number of animals showing the phenotype: 4 (22 dpi)
    Days post injection



    Fertility
    egg production: number <50% of expected -
    Comment: No ovary phenotype!
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003857 RA
      MPTPSYSHQ IVFNPLWLT LNSRGHHVT LFTTDPIKN 
      ANLSNFRQI DWSFAYQLW HEKHNVSDI IRNFNTNLL 
      KCLNQYVEM MNDIIIQEL EHPQVQEII NDKNEHFDL 
      VVVEFLNPA MVAFSKRFN CPFIGMSPM DLTNFVHEA 
      LGNPISPAL FPDFISRYD GEMNFFERV LNTFYYVGS 
      KLYFKYYFY PKIDEIIKE YFGEDVPPL EQMQRNASM 
      VFLNTNPII HNIRPLMSN VLMVGGGTH FEGDTPLPE 
      DIQKFLDGA ENGAIYFSL GTNVKSKDL DQDTKTTFL 
      QVFSELPYK VLWKFEDAK HPKIKLFIT QGGLQSLEE 
      AIYNGIPII GMPVYVDQY SNVKRAIRK GMGIILDSN 
      NVGKEILKK SIEDILNNE KYKKSAEKL SILLKEQPV 
      SGLNQTVKW IEYVLKYKG AKFLKPAQI PTYQYLLID 
      VLTFFVALF IFSAYFSFV CLKLLVKCM KERRRVKTT 
      KLE
    CDS sequence
    • >TC003857 RA
      atgccaacc ccttcatac agccaccaa atcgtcttc 
      aatcccctc tggctcaca ctgaactca agagggcac 
      cacgtcacc ttattcacc accgatcca atcaaaaac 
      gccaattta tcaaacttt cggcagatt gattggagc 
      ttcgcttac caattatgg cacgaaaag cacaacgtc 
      agcgacatc ataaggaac ttcaacaca aatttactc 
      aagtgttta aaccagtac gtggaaatg atgaacgac 
      atcatcata caagagctg gaacatccc caagtccag 
      gaaatcatc aatgacaaa aacgaacac tttgatttg 
      gtcgtcgtt gagttttta aatcccgca atggtggct 
      ttcagtaag agatttaac tgtccgttt attggaatg 
      tccccaatg gatttgacc aattttgta catgaagcc 
      ttgggtaat ccaatctct ccggctttg ttcccggat 
      tttatttct cgctacgat ggcgagatg aattttttt 
      gagcgagtc cttaacacg ttctactac gttggaagt 
      aaactctat ttcaagtat tatttttac ccgaaaatt 
      gatgagatt attaaggag tattttgga gaggatgtc 
      cctcctttg gaacagatg caacgcaat gccagcatg 
      gtttttctg aacacgaac ccaattatt cacaacata 
      aggccccta atgagcaac gtgttaatg gtaggtggg 
      ggaacacat tttgaggga gatactccc ctacctgaa 
      gatatacaa aaatttttg gacggggct gaaaacgga 
      gcgatttat ttcagtctt gggacaaat gttaaaagc 
      aaggatttg gaccaagac acgaaaaca acatttttg 
      caagtattt tccgaactg ccgtataaa gtgttatgg 
      aaattcgaa gacgcaaaa catccaaaa ataaaactc 
      ttcattact caaggaggt ctccagtct ttagaggaa 
      gcaatttac aacggaatt cccattatt gggatgcct 
      gtctatgtt gatcagtat tcaaacgtt aaaagggca 
      attcggaaa gggatggga attatcctt gatagtaat 
      aacgtgggg aaggaaata ctgaagaag tctatcgaa 
      gatatttta aataatgag aagtataaa aaatccgca 
      gaaaagttg tcaatttta ttgaaagag caacccgtt 
      tctgggctt aatcaaacc gtgaagtgg attgagtat 
      gtcttgaaa tataaagga gcgaaattt ttgaaacca 
      gctcaaatt ccaacgtat cagtatctc ctgattgat 
      gtgttaaca ttttttgta gcattgttt atattctct 
      gcttatttt tcgtttgtg tgtttgaaa ttacttgtc 
      aagtgtatg aaagaaaga cgtagggtt aaaacaacc 
      aagttagaa taa
    mRNA sequence
    • >TC003857 RA
      cgtgtatca agacaagaa atgttgcta aaacaactc 
      gttctttcc ctaatttta accaactta attcctccc 
      agtgccagt gaccgaatt ttagccata atgccaacc 
      ccttcatac agccaccaa atcgtcttc aatcccctc 
      tggctcaca ctgaactca agagggcac cacgtcacc 
      ttattcacc accgatcca atcaaaaac gccaattta 
      tcaaacttt cggcagatt gattggagc ttcgcttac 
      caattatgg cacgaaaag cacaacgtc agcgacatc 
      ataaggaac ttcaacaca aatttactc aagtgttta 
      aaccagtac gtggaaatg atgaacgac atcatcata 
      caagagctg gaacatccc caagtccag gaaatcatc 
      aatgacaaa aacgaacac tttgatttg gtcgtcgtt 
      gagttttta aatcccgca atggtggct ttcagtaag 
      agatttaac tgtccgttt attggaatg tccccaatg 
      gatttgacc aattttgta catgaagcc ttgggtaat 
      ccaatctct ccggctttg ttcccggat tttatttct 
      cgctacgat ggcgagatg aattttttt gagcgagtc 
      cttaacacg ttctactac gttggaagt aaactctat 
      ttcaagtat tatttttac ccgaaaatt gatgagatt 
      attaaggag tattttgga gaggatgtc cctcctttg 
      gaacagatg caacgcaat gccagcatg gtttttctg 
      aacacgaac ccaattatt cacaacata aggccccta 
      atgagcaac gtgttaatg gtaggtggg ggaacacat 
      tttgaggga gatactccc ctacctgaa gatatacaa 
      aaatttttg gacggggct gaaaacgga gcgatttat 
      ttcagtctt gggacaaat gttaaaagc aaggatttg 
      gaccaagac acgaaaaca acatttttg caagtattt 
      tccgaactg ccgtataaa gtgttatgg aaattcgaa 
      gacgcaaaa catccaaaa ataaaactc ttcattact 
      caaggaggt ctccagtct ttagaggaa gcaatttac 
      aacggaatt cccattatt gggatgcct gtctatgtt 
      gatcagtat tcaaacgtt aaaagggca attcggaaa 
      gggatggga attatcctt gatagtaat aacgtgggg 
      aaggaaata ctgaagaag tctatcgaa gatatttta 
      aataatgag aagtataaa aaatccgca gaaaagttg 
      tcaatttta ttgaaagag caacccgtt tctgggctt 
      aatcaaacc gtgaagtgg attgagtat gtcttgaaa 
      tataaagga gcgaaattt ttgaaacca gctcaaatt 
      ccaacgtat cagtatctc ctgattgat gtgttaaca 
      ttttttgta gcattgttt atattctct gcttatttt 
      tcgtttgtg tgtttgaaa ttacttgtc aagtgtatg 
      aaagaaaga cgtagggtt aaaacaacc aagttagaa 
      taaactctt ttttcgtgt acttaattt gataagat