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Gene information
General information
TC003856 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid23: 833776..847419
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003856
    iBeetle screen iB_03636
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003856 RA
      MDCLVAYYT ERFKTVDGN VFEEFAKFY EKDLTRVHS 
      WLESQFKKK TVKNNAISA LHRREGNNF YAEKNLIKS 
      FDCYTKSLR FAIPYSKEY GLALANRSA VSFELKEYH 
      NCLKDIELC FKSDYPTDL KPKVYVRKT ECYYELNQK 
      EALNQCISE VLHFLETEN VVEKGKFVT RLNHIKKLK 
      NAKPKTDSG QECLHEIPV FPDGENKCL AYASTKVKM 
      SYNKDKGRH IIASTNIQR GDLLFIEKA FVFAPVFKE 
      NKEFYSFKC YNCLSDIVS GVPCNTCTV CVYCDEKCR 
      DQNWTKCHR WECDGMQAN IWFDLGIAL PAFKVMLEG 
      ALSGFQTLE GGFEDDVKH FGDKENNYR YFNRLLSNI 
      HKNKNLAPY IIMASVVTT YLQKYTDFF TWFLTQPSC 
      PKEGLNELV KLVGGFITK HIAQLACNS STIEQWTCS 
      SSDLLFPDV LITIASGIF PSVSIMNHS CRPNVTNYF 
      MSDTIVVKA LEDIAQNEE IFNCYGIDY RGMEREQRQ 
      YACKELYHF ECKCVICSD PAHELDMLD SYLCPKCKG 
      LVPEIKNTI LSFCVNCGE KYHLKPFRK INYEAQKYM 
      ESDSSNQLE LLIKSLKIR EKILYKHHK DFEEVYYRL 
      YSFYVKLGD AENMFKYFH LWLENEKAR RGCNSRVVG 
      TKLYEAALA ILHCLKGGQ QKNCTNLKD FLQNVEYMI 
      KEAKAILNL YYPAFITNR LNKKIQFIS TK
    CDS sequence
    • >TC003856 RA
      atggattgt ttagtggct tattacacc gagaggttt 
      aaaactgtt gatggcaac gtgtttgaa gagtttgcc 
      aagttttat gaaaaagat ttgacacga gttcattcg 
      tggttagaa agtcagttt aagaaaaaa acggtgaaa 
      aataacgct atttcggcg ttacatagg cgagaaggg 
      aataatttt tatgctgag aagaatcta attaagagt 
      tttgattgt tatacaaag agtttacgt tttgccata 
      ccatacagt aaagagtat ggactagct ttggccaat 
      agatctgcc gtttcattt gaattgaaa gagtaccac 
      aactgtctt aaagatatt gagttgtgt ttcaagagt 
      gattatcca acagatctg aaaccaaaa gtttacgtt 
      aggaagacc gaatgttat tatgagctg aatcaaaaa 
      gaggctttg aatcagtgc atttctgag gtgctgcat 
      tttctagag actgaaaac gttgtggaa aaaggtaaa 
      tttgtcacg aggttaaac cacattaag aaattaaaa 
      aatgcaaaa ccaaaaact gacagtggt caagagtgc 
      cttcacgaa atcccggta tttcccgat ggagaaaac 
      aaatgtttg gcctatgct tccactaaa gtaaaaatg 
      agttacaat aaagacaaa ggacgtcac attattgct 
      agtacaaac atccaaaga ggcgacctc ctctttata 
      gaaaaagcg tttgttttc gccccagtt ttcaaagaa 
      aacaaggaa ttctactcg ttcaagtgt tacaactgt 
      cttagcgat attgtaagc ggtgtgcct tgcaacaca 
      tgcaccgtt tgtgtttac tgcgacgaa aaatgtcgc 
      gatcaaaat tggacaaag tgccatcgg tgggaatgc 
      gatggaatg caagccaac atttggttt gacttagga 
      attgcactt ccagcgttt aaagtcatg ttagaaggg 
      gctttatca gggtttcaa acactggaa ggtggcttt 
      gaagacgat gtgaaacat ttcggtgat aaagaaaac 
      aactatcgg tacttcaac cgcctgctt tccaacatc 
      cataagaac aaaaacctg gccccttat ataattatg 
      gccagtgtt gtgactacg tatttgcaa aaatacacc 
      gattttttc acctggttt ttgacacaa cccagctgt 
      ccaaaggag ggtttaaat gagttggtg aaactcgtc 
      gggggtttt attacaaaa cacatcgct cagcttgcg 
      tgcaattcg agcacgatc gaacagtgg acgtgctct 
      tcgtcggat ttgctcttc ccggatgtt ctcataacg 
      atagcttct gggattttt ccgtcagtt agtataatg 
      aaccattcg tgtcgacca aatgtcacg aattatttc 
      atgagtgat actattgtt gttaaagcg ctcgaggat 
      atcgctcaa aatgaggaa attttcaat tgttacgga 
      atcgattat cgggggatg gagagggag caaagacag 
      tacgcttgc aaggagttg taccatttc gaatgtaaa 
      tgtgtcatt tgctcggat cctgcacac gaactggac 
      atgctggac agctatttg tgcccaaaa tgcaaagga 
      ctggtccca gaaattaag aacacaatt ttgagtttt 
      tgcgttaac tgtggcgaa aaataccat ttgaaaccg 
      tttaggaaa atcaattat gaggcccaa aaatacatg 
      gaaagcgat tcttctaat cagttagaa cttttaata 
      aagagttta aaaatcaga gaaaaaatt ctctataaa 
      catcacaaa gatttcgag gaagtttac taccgactg 
      tatagtttt tacgtaaag cttggagac gctgaaaat 
      atgttcaaa tatttccac ttatggcta gagaatgag 
      aaagcgcgt cggggttgc aactcaagg gttgtaggg 
      acaaagtta tacgaagct gcgcttgca atccttcat 
      tgtttgaag gggggacag caaaaaaat tgcacaaat 
      ctcaaggat tttctacaa aatgtcgaa tatatgatt 
      aaagaagcc aaagccatc ctgaattta tattatcca 
      gcgttcatc acaaacagg ctgaataag aaaatacaa 
      tttatttcg acgaaataa
    mRNA sequence
    • >TC003856 RA
      tcttttgta ccatttcaa tcacgagtt ttgtcaccg 
      gcgaagtga tggatgtca ttagtgaca tgtgttatt 
      tattacgca aatatttca aaaagtttc agtttttgt 
      gctttagac ttcaacaca ttgtgttct aattttttt 
      caatacgta cgaacattt tgtagtttt ttaaaataa 
      tctacaaat ccagaaatg gattgttta gtggcttat 
      tacaccgag aggtttaaa actgttgat ggcaacgtg 
      tttgaagag tttgccaag ttttatgaa aaagatttg 
      acacgagtt cattcgtgg ttagaaagt cagtttaag 
      aaaaaaacg gtgaaaaat aacgctatt tcggcgtta 
      cataggcga gaagggaat aatttttat gctgagaag 
      aatctaatt aagagtttt gattgttat acaaagagt 
      ttacgtttt gccatacca tacagtaaa gagtatgga 
      ctagctttg gccaataga tctgccgtt tcatttgaa 
      ttgaaagag taccacaac tgtcttaaa gatattgag 
      ttgtgtttc aagagtgat tatccaaca gatctgaaa 
      ccaaaagtt tacgttagg aagaccgaa tgttattat 
      gagctgaat caaaaagag gctttgaat cagtgcatt 
      tctgaggtg ctgcatttt ctagagact gaaaacgtt 
      gtggaaaaa ggtaaattt gtcacgagg ttaaaccac 
      attaagaaa ttaaaaaat gcaaaacca aaaactgac 
      agtggtcaa gagtgcctt cacgaaatc ccggtattt 
      cccgatgga gaaaacaaa tgtttggcc tatgcttcc 
      actaaagta aaaatgagt tacaataaa gacaaagga 
      cgtcacatt attgctagt acaaacatc caaagaggc 
      gacctcctc tttatagaa aaagcgttt gttttcgcc 
      ccagttttc aaagaaaac aaggaattc tactcgttc 
      aagtgttac aactgtctt agcgatatt gtaagcggt 
      gtgccttgc aacacatgc accgtttgt gtttactgc 
      gacgaaaaa tgtcgcgat caaaattgg acaaagtgc 
      catcggtgg gaatgcgat ggaatgcaa gccaacatt 
      tggtttgac ttaggaatt gcacttcca gcgtttaaa 
      gtcatgtta gaaggggct ttatcaggg tttcaaaca 
      ctggaaggt ggctttgaa gacgatgtg aaacatttc 
      ggtgataaa gaaaacaac tatcggtac ttcaaccgc 
      ctgctttcc aacatccat aagaacaaa aacctggcc 
      ccttatata attatggcc agtgttgtg actacgtat 
      ttgcaaaaa tacaccgat tttttcacc tggtttttg 
      acacaaccc agctgtcca aaggagggt ttaaatgag 
      ttggtgaaa ctcgtcggg ggttttatt acaaaacac 
      atcgctcag cttgcgtgc aattcgagc acgatcgaa 
      cagtggacg tgctcttcg tcggatttg ctcttcccg 
      gatgttctc ataacgata gcttctggg atttttccg 
      tcagttagt ataatgaac cattcgtgt cgaccaaat 
      gtcacgaat tatttcatg agtgatact attgttgtt 
      aaagcgctc gaggatatc gctcaaaat gaggaaatt 
      ttcaattgt tacggaatc gattatcgg gggatggag 
      agggagcaa agacagtac gcttgcaag gagttgtac 
      catttcgaa tgtaaatgt gtcatttgc tcggatcct 
      gcacacgaa ctggacatg ctggacagc tatttgtgc 
      ccaaaatgc aaaggactg gtcccagaa attaagaac 
      acaattttg agtttttgc gttaactgt ggcgaaaaa 
      taccatttg aaaccgttt aggaaaatc aattatgag 
      gcccaaaaa tacatggaa agcgattct tctaatcag 
      ttagaactt ttaataaag agtttaaaa atcagagaa 
      aaaattctc tataaacat cacaaagat ttcgaggaa 
      gtttactac cgactgtat agtttttac gtaaagctt 
      ggagacgct gaaaatatg ttcaaatat ttccactta 
      tggctagag aatgagaaa gcgcgtcgg ggttgcaac 
      tcaagggtt gtagggaca aagttatac gaagctgcg 
      cttgcaatc cttcattgt ttgaagggg ggacagcaa 
      aaaaattgc acaaatctc aaggatttt ctacaaaat 
      gtcgaatat atgattaaa gaagccaaa gccatcctg 
      aatttatat tatccagcg ttcatcaca aacaggctg 
      aataagaaa atacaattt atttcgacg aaataaaaa 
      aatgtgtta ctactcttt