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Gene information
General information
TC003854 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 23286350..23304725
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003854
    iBeetle screen iB_03635

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    remarks: later than schedule, hours 48 remarks: later than schedule, hours 48 remarks: later than schedule, hours 48
    Pupal morphology
    remarks: later than schedule, hours 48
    Adult morphology
    remarks: later than schedule, hours 48 remarks: later than schedule, hours 48
    Fertility
    mature egg deposition 3 number 2nd lateral oviduct: number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 5
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: later than schedule, hours 48 (egglay)
    remarks: later than schedule, hours 48
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    remarks: later than schedule, hours 48
    remarks - cuticles: later than schedule, hours 48
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003854 RA
      MDYSEDYDG YDDVYGHSV DDDYYISPS NRQFIYNRE 
      NSRDQPKIG EFIRTEQDI QEEDESETA SDLSDLDKA 
      RLESCIEQI KATLGPLCI PRNELVNII ISNNYNIER 
      ATNAVLDDS KNLQSEPKE KVPSIKVVA SGGKTTTNV 
      TKGFDLSQN KDSIKLNTT PRSQSPASG RGTPIISQS 
      EGTSEETKI IKSKENKID VETQYKKER GDSKEHLYM 
      VVIGHVDAG KSTLMGHLL YDLGQVNQK TMHKYEQES 
      RKVGKQSFM YAWVLDETG EERNRGITM DVGRSQFET 
      KSKHVTLLD APGHKDFIP NMISGAGQA DVALLVVDA 
      TRGEFETGF DFGGQTREH ALLVRSLGV TQLAVAINK 
      LDTVSWSKE RFDDISQKL KVFLKQAGF REGDVTFVP 
      CSGLTGQNL VDKPTENEL LTWYNGPCL LEVIDNFRT 
      PERPVSKPF RLSINDIFK GTGSGFCVS GRVETGSLN 
      VGERVMVCP SRELSMVKS LYIEDLSQT VVFAGDQAT 
      VTLSGIEMQ NVSIGNVLC DPQNPVQVS AKFQARIVV 
      FNLTIPITK GFSVILHHQ SLVEPAVVS KLISQLNRS 
      TGEVVKKHP RFLSNNTSA IVEIQVSRP IALELYSDC 
      KELGRFMLR VGGVTIAAG LITKIIL
    • >TC003854 RB
      MDYSEDYDG YDDVYGHSV DDDYYISPS NRQFIYNRE 
      NSRDQPKIG EFIRTEQDI QEEDESETA SDLSDLDKA 
      RLESCIEQI KATLGPLCI PRNELVNII ISNNYNIER 
      ATNAVLDDS KNLQSEPKE KDVTNSSKF FVPKLNGFK 
      ENIKSPFTP PRFNIPSLT GSQTFTIPK LNFPKDNTD 
      TSKKFDSLS DLVSNHLSK TLKNVSLNS TESVTQVTQ 
      NIKNLNIQP EIQKSASEY RIDLSVALK SSPSSHSLP 
      NRSKHDLTE EKFEIPFVD CDVDVESKS ALKSAFDAK 
      DVLNVCLYN VKPVSTFGK VLCRKYRRR NSIKFKRSK 
      CVIDFTKIA IFNFATPSP DDNIIKHLK S
    • >TC003854 RC
      MVVIGHVDA GKSTLMGHL LYDLGQVNQ KTMHKYEQE 
      SRKVGKQSF MYAWVLDET GEERNRGIT MDVGRSQFE 
      TKSKHVTLL DAPGHKDFI PNMISGAGQ ADVALLVVD 
      ATRGEFETG FDFGGQTRE HALLVRSLG VTQLAVAIN 
      KLDTVSWSK ERFDDISQK LKVFLKQAG FREGDVTFV 
      PCSGLTGQN LVDKPTENE LLTWYNGPC LLEVIDNFR 
      TPERPVSKP FRLSINDIF KGTGSGFCV SGRVETGSL 
      NVGERVMVC PSRELSMVK SLYIEDLSQ TVVFAGDQA 
      TVTLSGIEM QNVSIGNVL CDPQNPVQV SAKFQARIV 
      VFNLTIPIT KGFSVILHH QSLVEPAVV SKLISQLNR 
      STGEVVKKH PRFLSNNTS AIVEIQVSR PIALELYSD 
      CKELGRFML RVGGVTIAA GLITKIIL
    CDS sequence
    • >TC003854 RA
      atggattat tccgaagac tatgatggt tatgacgac 
      gtttacggc cattccgtc gacgatgat tactatatt 
      tccccaagt aatcgacag tttatctat aatcgagag 
      aactcacga gatcaacca aaaattggc gagtttatc 
      cgcacggaa caagacatc caagaggaa gacgaatca 
      gaaacggcg tcagatttg tcagattta gataaggca 
      aggttggag tcttgcatt gagcaaatt aaagccact 
      ttgggcccc ttgtgcatt cctcgaaac gagttagta 
      aacataata atttcaaac aattacaac atcgaacga 
      gccactaat gcagtactg gacgacagt aagaacctg 
      cagagtgaa ccgaaggaa aaagtgccc agcataaaa 
      gtggtggca tcggggggt aaaacaacc acaaacgtc 
      acaaaaggt ttcgatctt agccaaaat aaggattcc 
      attaaacta aataccact ccacgttca caatcacct 
      gcttctggt cgaggaaca ccgattata tctcagtct 
      gaaggcact tcggaagaa acgaaaatt attaaaagt 
      aaagaaaat aaaattgat gttgaaaca caatacaaa 
      aaggagaga ggcgattcg aaagaacat ctttatatg 
      gtggttatt ggtcacgtg gatgctgga aaatcgact 
      ttgatgggg cacttgctc tatgaccta ggacaggtg 
      aatcaaaaa acaatgcac aaatatgaa caagagagc 
      cgaaaggtg ggtaaacaa agcttcatg tatgcgtgg 
      gttttggac gaaactggt gaggaaaga aacagaggt 
      ataacgatg gacgtcggc cgttcccaa ttcgaaaca 
      aaatcaaaa catgtcaca ttacttgac gcccccggc 
      cacaaggat ttcatccct aatatgata tcaggggcg 
      ggccaagct gacgttgct ctacttgta gtggacgca 
      accagaggt gaattcgag actggattc gatttcggt 
      ggtcaaacg cgagagcat gcacttctt gttagatca 
      ctaggagtc actcaactt gctgtggcc atcaacaaa 
      ttggatacg gtttcgtgg tccaaagaa cgatttgat 
      gatatctca caaaaattg aaagttttt ctcaaacaa 
      gctgggttt agggaaggc gatgttacg tttgttcct 
      tgtagtggg ttgactggg caaaatttg gtcgacaaa 
      ccgacggaa aacgagctt ttgacttgg tacaacggg 
      ccttgttta ttggaagtg atcgataat ttccgtact 
      cccgaacgc ccagtctcc aaaccgttc cgcctatcc 
      atcaatgac attttcaaa gggactggt tctggattt 
      tgtgtatca ggtcgggtt gaaacgggc agtttgaat 
      gtaggagag cgggtcatg gtgtgcccc agtcgcgaa 
      ctctcaatg gtcaaatcg ctctacatc gaagattta 
      tcacaaacg gtggttttt gccggagac caagccact 
      gttacttta tccggtatt gaaatgcaa aatgtttcc 
      attggaaac gtgctctgc gaccctcag aaccccgtc 
      caagtgtcg gccaagttc caggcaagg attgttgtt 
      ttcaacttg actattcca ataactaag ggattttcg 
      gttatttta catcatcag tctttagtc gagccggcg 
      gtggttagt aaactgata tcgcagcta aataggagt 
      acaggggaa gtggtgaag aaacatccg agatttttg 
      agtaacaat acaagtgct attgttgag attcaagtt 
      tcgaggccc attgcgctt gaactgtac agtgattgt 
      aaggagctg gggaggttt atgctgagg gttggcggg 
      gttacgata gctgcagga ctcattaca aaaattata 
      ttgtag
    • >TC003854 RB
      atggattat tccgaagac tatgatggt tatgacgac 
      gtttacggc cattccgtc gacgatgat tactatatt 
      tccccaagt aatcgacag tttatctat aatcgagag 
      aactcacga gatcaacca aaaattggc gagtttatc 
      cgcacggaa caagacatc caagaggaa gacgaatca 
      gaaacggcg tcagatttg tcagattta gataaggca 
      aggttggag tcttgcatt gagcaaatt aaagccact 
      ttgggcccc ttgtgcatt cctcgaaac gagttagta 
      aacataata atttcaaac aattacaac atcgaacga 
      gccactaat gcagtactg gacgacagt aagaacctg 
      cagagtgaa ccgaaggaa aaagatgta acaaacagt 
      tcaaaattt tttgtgcca aaattgaac ggcttcaaa 
      gaaaacatt aagtcgccc ttcacaccc ccgcgtttt 
      aacattcct tcactaact ggatctcaa acattcaca 
      ataccaaaa cttaatttc cctaaagac aataccgac 
      acaagcaaa aagtttgat agtctgtcc gatttagtc 
      tcaaaccac ttgagcaaa acgctcaaa aatgtctca 
      cttaactca accgagagc gttactcaa gtcacccaa 
      aatatcaaa aatttgaac attcagcct gaaatacaa 
      aaatcagca agtgaatat cgaattgat ctctcagtt 
      gcgctaaaa tcgtctcca agttcccac agtttgccg 
      aatcgaagt aaacacgat ttgactgag gaaaagttt 
      gagattcct tttgttgac tgtgacgtc gatgtggaa 
      agtaaaagt gcgttgaag tctgcgttt gatgctaaa 
      gatgtgcta aatgtgtgt ctttataac gtgaaaccg 
      gtctcgact tttggtaaa gttttgtgc cggaaatac 
      cggagacgg aattcgatt aagtttaaa cgaagtaaa 
      tgtgtgatt gattttacg aaaattgca atctttaat 
      tttgccact ccttcgccc gatgataat ataattaaa 
      cacttgaaa agttga
    • >TC003854 RC
      atggtggtt attggtcac gtggatgct ggaaaatcg 
      actttgatg gggcacttg ctctatgac ctaggacag 
      gtgaatcaa aaaacaatg cacaaatat gaacaagag 
      agccgaaag gtgggtaaa caaagcttc atgtatgcg 
      tgggttttg gacgaaact ggtgaggaa agaaacaga 
      ggtataacg atggacgtc ggccgttcc caattcgaa 
      acaaaatca aaacatgtc acattactt gacgccccc 
      ggccacaag gatttcatc cctaatatg atatcaggg 
      gcgggccaa gctgacgtt gctctactt gtagtggac 
      gcaaccaga ggtgaattc gagactgga ttcgatttc 
      ggtggtcaa acgcgagag catgcactt cttgttaga 
      tcactagga gtcactcaa cttgctgtg gccatcaac 
      aaattggat acggtttcg tggtccaaa gaacgattt 
      gatgatatc tcacaaaaa ttgaaagtt tttctcaaa 
      caagctggg tttagggaa ggcgatgtt acgtttgtt 
      ccttgtagt gggttgact gggcaaaat ttggtcgac 
      aaaccgacg gaaaacgag cttttgact tggtacaac 
      gggccttgt ttattggaa gtgatcgat aatttccgt 
      actcccgaa cgcccagtc tccaaaccg ttccgccta 
      tccatcaat gacattttc aaagggact ggttctgga 
      ttttgtgta tcaggtcgg gttgaaacg ggcagtttg 
      aatgtagga gagcgggtc atggtgtgc cccagtcgc 
      gaactctca atggtcaaa tcgctctac atcgaagat 
      ttatcacaa acggtggtt tttgccgga gaccaagcc 
      actgttact ttatccggt attgaaatg caaaatgtt 
      tccattgga aacgtgctc tgcgaccct cagaacccc 
      gtccaagtg tcggccaag ttccaggca aggattgtt 
      gttttcaac ttgactatt ccaataact aagggattt 
      tcggttatt ttacatcat cagtcttta gtcgagccg 
      gcggtggtt agtaaactg atatcgcag ctaaatagg 
      agtacaggg gaagtggtg aagaaacat ccgagattt 
      ttgagtaac aatacaagt gctattgtt gagattcaa 
      gtttcgagg cccattgcg cttgaactg tacagtgat 
      tgtaaggag ctggggagg tttatgctg agggttggc 
      ggggttacg atagctgca ggactcatt acaaaaatt 
      atattgtag
    mRNA sequence
    • >TC003854 RA
      cgtcaacca aaatattaa aatgtcagc tccaaagtt 
      gtgaaaagt gcaatctgt tatttaaca cattttaaa 
      aactttcag tgaataaaa cacacgaaa aaatgtctc 
      gacatcgag atgttcgca atatggatt attccgaag 
      actatgatg gttatgacg acgtttacg gccattccg 
      tcgacgatg attactata tttccccaa gtaatcgac 
      agtttatct ataatcgag agaactcac gagatcaac 
      caaaaattg gcgagttta tccgcacgg aacaagaca 
      tccaagagg aagacgaat cagaaacgg cgtcagatt 
      tgtcagatt tagataagg caaggttgg agtcttgca 
      ttgagcaaa ttaaagcca ctttgggcc ccttgtgca 
      ttcctcgaa acgagttag taaacataa taatttcaa 
      acaattaca acatcgaac gagccacta atgcagtac 
      tggacgaca gtaagaacc tgcagagtg aaccgaagg 
      aaaaagtgc ccagcataa aagtggtgg catcggggg 
      gtaaaacaa ccacaaacg tcacaaaag gtttcgatc 
      ttagccaaa ataaggatt ccattaaac taaatacca 
      ctccacgtt cacaatcac ctgcttctg gtcgaggaa 
      caccgatta tatctcagt ctgaaggca cttcggaag 
      aaacgaaaa ttattaaaa gtaaagaaa ataaaattg 
      atgttgaaa cacaataca aaaaggaga gaggcgatt 
      cgaaagaac atctttata tggtggtta ttggtcacg 
      tggatgctg gaaaatcga ctttgatgg ggcacttgc 
      tctatgacc taggacagg tgaatcaaa aaacaatgc 
      acaaatatg aacaagaga gccgaaagg tgggtaaac 
      aaagcttca tgtatgcgt gggttttgg acgaaactg 
      gtgaggaaa gaaacagag gtataacga tggacgtcg 
      gccgttccc aattcgaaa caaaatcaa aacatgtca 
      cattacttg acgcccccg gccacaagg atttcatcc 
      ctaatatga tatcagggg cgggccaag ctgacgttg 
      ctctacttg tagtggacg caaccagag gtgaattcg 
      agactggat tcgatttcg gtggtcaaa cgcgagagc 
      atgcacttc ttgttagat cactaggag tcactcaac 
      ttgctgtgg ccatcaaca aattggata cggtttcgt 
      ggtccaaag aacgatttg atgatatct cacaaaaat 
      tgaaagttt ttctcaaac aagctgggt ttagggaag 
      gcgatgtta cgtttgttc cttgtagtg ggttgactg 
      ggcaaaatt tggtcgaca aaccgacgg aaaacgagc 
      ttttgactt ggtacaacg ggccttgtt tattggaag 
      tgatcgata atttccgta ctcccgaac gcccagtct 
      ccaaaccgt tccgcctat ccatcaatg acattttca 
      aagggactg gttctggat tttgtgtat caggtcggg 
      ttgaaacgg gcagtttga atgtaggag agcgggtca 
      tggtgtgcc ccagtcgcg aactctcaa tggtcaaat 
      cgctctaca tcgaagatt tatcacaaa cggtggttt 
      ttgccggag accaagcca ctgttactt tatccggta 
      ttgaaatgc aaaatgttt ccattggaa acgtgctct 
      gcgaccctc agaaccccg tccaagtgt cggccaagt 
      tccaggcaa ggattgttg ttttcaact tgactattc 
      caataacta agggatttt cggttattt tacatcatc 
      agtctttag tcgagccgg cggtggtta gtaaactga 
      tatcgcagc taaatagga gtacagggg aagtggtga 
      agaaacatc cgagatttt tgagtaaca atacaagtg 
      ctattgttg agattcaag tttcgaggc ccattgcgc 
      ttgaactgt acagtgatt gtaaggagc tggggaggt 
      ttatgctga gggttggcg gggttacga tagctgcag 
      gactcatta caaaaatta tattgtaga gtaaggatg 
      aataaaagt cgaaatttt at
    • >TC003854 RB
      cgtcaacca aaatattaa aatgtcagc tccaaagtt 
      gtgaaaagt gcaatctgt tatttaaca cattttaaa 
      aactttcag tgaataaaa cacacgaaa aaatgtctc 
      gacatcgag atgttcgca atatggatt attccgaag 
      actatgatg gttatgacg acgtttacg gccattccg 
      tcgacgatg attactata tttccccaa gtaatcgac 
      agtttatct ataatcgag agaactcac gagatcaac 
      caaaaattg gcgagttta tccgcacgg aacaagaca 
      tccaagagg aagacgaat cagaaacgg cgtcagatt 
      tgtcagatt tagataagg caaggttgg agtcttgca 
      ttgagcaaa ttaaagcca ctttgggcc ccttgtgca 
      ttcctcgaa acgagttag taaacataa taatttcaa 
      acaattaca acatcgaac gagccacta atgcagtac 
      tggacgaca gtaagaacc tgcagagtg aaccgaagg 
      aaaaagatg taacaaaca gttcaaaat tttttgtgc 
      caaaattga acggcttca aagaaaaca ttaagtcgc 
      ccttcacac ccccgcgtt ttaacattc cttcactaa 
      ctggatctc aaacattca caataccaa aacttaatt 
      tccctaaag acaataccg acacaagca aaaagtttg 
      atagtctgt ccgatttag tctcaaacc acttgagca 
      aaacgctca aaaatgtct cacttaact caaccgaga 
      gcgttactc aagtcaccc aaaatatca aaaatttga 
      acattcagc ctgaaatac aaaaatcag caagtgaat 
      atcgaattg atctctcag ttgcgctaa aatcgtctc 
      caagttccc acagtttgc cgaatcgaa gtaaacacg 
      atttgactg aggaaaagt ttgagattc cttttgttg 
      actgtgacg tcgatgtgg aaagtaaaa gtgcgttga 
      agtctgcgt ttgatgcta aagatgtgc taaatgtgt 
      gtctttata acgtgaaac cggtctcga cttttggta 
      aagttttgt gccggaaat accggagac ggaattcga 
      ttaagttta aacgaagta aatgtgtga ttgatttta 
      cgaaaattg caatcttta attttgcca ctccttcgc 
      ccgatgata atataatta aacacttga aaagttgac 
      gacgcaagt tttcttgtg gtttttgta gtttagctg 
      acggatcaa aatgtacat tctgtaaga ataacgtaa 
      taaataatc attattttt agttacaaa aactcgtgt 
      acatttata attttaatt ttacatttt catgtttta 
      cgtatttta taaggttgg ataaaaggg aaatttata 
      cataaagac aaataaata taatactca tac
    • >TC003854 RC
      ttagtaaag aaaatgaaa aaatgggaa tctaaaata 
      ctttgcaaa aaattcacg aaacggagt aaatttcat 
      gttgtgtta tacataaag cacacatta caatacctg 
      aaaaattga agtttgact aagaattct tgactcgtt 
      tgaatatgt tttcttatt gcattaaac gtaaacaat 
      ttaatttat aaacatgtt tacaacacg tttcattat 
      gttatgttt tcgttattt tttgccatt tattgtgat 
      actgagaac aaatcttga agggcaact tgaatatag 
      ttcacttat tttaattat atttacttg gagggtttt 
      tcaaatcca gtaaaataa atttctgtc gctatatct 
      aaagtgccc agcataaaa gtggtggca tcggggggt 
      aaaacaacc acaaacgtc acaaaaggt ttcgatctt 
      agccaaaat aaggattcc attaaacta aataccact 
      ccacgttca caatcacct gcttctggt cgaggaaca 
      ccgattata tctcagtct gaaggcact tcggaagaa 
      acgaaaatt attaaaagt aaagaaaat aaaattgat 
      gttgaaaca caatacaaa aaggagaga ggcgattcg 
      aaagaacat ctttatatg gtggttatt ggtcacgtg 
      gatgctgga aaatcgact ttgatgggg cacttgctc 
      tatgaccta ggacaggtg aatcaaaaa acaatgcac 
      aaatatgaa caagagagc cgaaaggtg ggtaaacaa 
      agcttcatg tatgcgtgg gttttggac gaaactggt 
      gaggaaaga aacagaggt ataacgatg gacgtcggc 
      cgttcccaa ttcgaaaca aaatcaaaa catgtcaca 
      ttacttgac gcccccggc cacaaggat ttcatccct 
      aatatgata tcaggggcg ggccaagct gacgttgct 
      ctacttgta gtggacgca accagaggt gaattcgag 
      actggattc gatttcggt ggtcaaacg cgagagcat 
      gcacttctt gttagatca ctaggagtc actcaactt 
      gctgtggcc atcaacaaa ttggatacg gtttcgtgg 
      tccaaagaa cgatttgat gatatctca caaaaattg 
      aaagttttt ctcaaacaa gctgggttt agggaaggc 
      gatgttacg tttgttcct tgtagtggg ttgactggg 
      caaaatttg gtcgacaaa ccgacggaa aacgagctt 
      ttgacttgg tacaacggg ccttgttta ttggaagtg 
      atcgataat ttccgtact cccgaacgc ccagtctcc 
      aaaccgttc cgcctatcc atcaatgac attttcaaa 
      gggactggt tctggattt tgtgtatca ggtcgggtt 
      gaaacgggc agtttgaat gtaggagag cgggtcatg 
      gtgtgcccc agtcgcgaa ctctcaatg gtcaaatcg 
      ctctacatc gaagattta tcacaaacg gtggttttt 
      gccggagac caagccact gttacttta tccggtatt 
      gaaatgcaa aatgtttcc attggaaac gtgctctgc 
      gaccctcag aaccccgtc caagtgtcg gccaagttc 
      caggcaagg attgttgtt ttcaacttg actattcca 
      ataactaag ggattttcg gttatttta catcatcag 
      tctttagtc gagccggcg gtggttagt aaactgata 
      tcgcagcta aataggagt acaggggaa gtggtgaag 
      aaacatccg agatttttg agtaacaat acaagtgct 
      attgttgag attcaagtt tcgaggccc attgcgctt 
      gaactgtac agtgattgt aaggagctg gggaggttt 
      atgctgagg gttggcggg gttacgata gctgcagga 
      ctcattaca aaaattata ttgtagagt aaggatgaa 
      taaaagtcg aaattttat
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