| General information | |||
| TC003854 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 23286350..23304725 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC003854 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03635 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)remarks: later than schedule, hours 48 remarks: later than schedule, hours 48 remarks: later than schedule, hours 48
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: later than schedule, hours 48 (egglay)
remarks: later than schedule, hours 48
remarks: later than schedule, hours 48
remarks - cuticles: later than schedule, hours 48
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
- >TC003854 RA
MDYSEDYDG YDDVYGHSV DDDYYISPS NRQFIYNRE NSRDQPKIG EFIRTEQDI QEEDESETA SDLSDLDKA RLESCIEQI KATLGPLCI PRNELVNII ISNNYNIER ATNAVLDDS KNLQSEPKE KVPSIKVVA SGGKTTTNV TKGFDLSQN KDSIKLNTT PRSQSPASG RGTPIISQS EGTSEETKI IKSKENKID VETQYKKER GDSKEHLYM VVIGHVDAG KSTLMGHLL YDLGQVNQK TMHKYEQES RKVGKQSFM YAWVLDETG EERNRGITM DVGRSQFET KSKHVTLLD APGHKDFIP NMISGAGQA DVALLVVDA TRGEFETGF DFGGQTREH ALLVRSLGV TQLAVAINK LDTVSWSKE RFDDISQKL KVFLKQAGF REGDVTFVP CSGLTGQNL VDKPTENEL LTWYNGPCL LEVIDNFRT PERPVSKPF RLSINDIFK GTGSGFCVS GRVETGSLN VGERVMVCP SRELSMVKS LYIEDLSQT VVFAGDQAT VTLSGIEMQ NVSIGNVLC DPQNPVQVS AKFQARIVV FNLTIPITK GFSVILHHQ SLVEPAVVS KLISQLNRS TGEVVKKHP RFLSNNTSA IVEIQVSRP IALELYSDC KELGRFMLR VGGVTIAAG LITKIIL
- >TC003854 RB
MDYSEDYDG YDDVYGHSV DDDYYISPS NRQFIYNRE NSRDQPKIG EFIRTEQDI QEEDESETA SDLSDLDKA RLESCIEQI KATLGPLCI PRNELVNII ISNNYNIER ATNAVLDDS KNLQSEPKE KDVTNSSKF FVPKLNGFK ENIKSPFTP PRFNIPSLT GSQTFTIPK LNFPKDNTD TSKKFDSLS DLVSNHLSK TLKNVSLNS TESVTQVTQ NIKNLNIQP EIQKSASEY RIDLSVALK SSPSSHSLP NRSKHDLTE EKFEIPFVD CDVDVESKS ALKSAFDAK DVLNVCLYN VKPVSTFGK VLCRKYRRR NSIKFKRSK CVIDFTKIA IFNFATPSP DDNIIKHLK S
- >TC003854 RC
MVVIGHVDA GKSTLMGHL LYDLGQVNQ KTMHKYEQE SRKVGKQSF MYAWVLDET GEERNRGIT MDVGRSQFE TKSKHVTLL DAPGHKDFI PNMISGAGQ ADVALLVVD ATRGEFETG FDFGGQTRE HALLVRSLG VTQLAVAIN KLDTVSWSK ERFDDISQK LKVFLKQAG FREGDVTFV PCSGLTGQN LVDKPTENE LLTWYNGPC LLEVIDNFR TPERPVSKP FRLSINDIF KGTGSGFCV SGRVETGSL NVGERVMVC PSRELSMVK SLYIEDLSQ TVVFAGDQA TVTLSGIEM QNVSIGNVL CDPQNPVQV SAKFQARIV VFNLTIPIT KGFSVILHH QSLVEPAVV SKLISQLNR STGEVVKKH PRFLSNNTS AIVEIQVSR PIALELYSD CKELGRFML RVGGVTIAA GLITKIIL
- >TC003854 RA
atggattat tccgaagac tatgatggt tatgacgac gtttacggc cattccgtc gacgatgat tactatatt tccccaagt aatcgacag tttatctat aatcgagag aactcacga gatcaacca aaaattggc gagtttatc cgcacggaa caagacatc caagaggaa gacgaatca gaaacggcg tcagatttg tcagattta gataaggca aggttggag tcttgcatt gagcaaatt aaagccact ttgggcccc ttgtgcatt cctcgaaac gagttagta aacataata atttcaaac aattacaac atcgaacga gccactaat gcagtactg gacgacagt aagaacctg cagagtgaa ccgaaggaa aaagtgccc agcataaaa gtggtggca tcggggggt aaaacaacc acaaacgtc acaaaaggt ttcgatctt agccaaaat aaggattcc attaaacta aataccact ccacgttca caatcacct gcttctggt cgaggaaca ccgattata tctcagtct gaaggcact tcggaagaa acgaaaatt attaaaagt aaagaaaat aaaattgat gttgaaaca caatacaaa aaggagaga ggcgattcg aaagaacat ctttatatg gtggttatt ggtcacgtg gatgctgga aaatcgact ttgatgggg cacttgctc tatgaccta ggacaggtg aatcaaaaa acaatgcac aaatatgaa caagagagc cgaaaggtg ggtaaacaa agcttcatg tatgcgtgg gttttggac gaaactggt gaggaaaga aacagaggt ataacgatg gacgtcggc cgttcccaa ttcgaaaca aaatcaaaa catgtcaca ttacttgac gcccccggc cacaaggat ttcatccct aatatgata tcaggggcg ggccaagct gacgttgct ctacttgta gtggacgca accagaggt gaattcgag actggattc gatttcggt ggtcaaacg cgagagcat gcacttctt gttagatca ctaggagtc actcaactt gctgtggcc atcaacaaa ttggatacg gtttcgtgg tccaaagaa cgatttgat gatatctca caaaaattg aaagttttt ctcaaacaa gctgggttt agggaaggc gatgttacg tttgttcct tgtagtggg ttgactggg caaaatttg gtcgacaaa ccgacggaa aacgagctt ttgacttgg tacaacggg ccttgttta ttggaagtg atcgataat ttccgtact cccgaacgc ccagtctcc aaaccgttc cgcctatcc atcaatgac attttcaaa gggactggt tctggattt tgtgtatca ggtcgggtt gaaacgggc agtttgaat gtaggagag cgggtcatg gtgtgcccc agtcgcgaa ctctcaatg gtcaaatcg ctctacatc gaagattta tcacaaacg gtggttttt gccggagac caagccact gttacttta tccggtatt gaaatgcaa aatgtttcc attggaaac gtgctctgc gaccctcag aaccccgtc caagtgtcg gccaagttc caggcaagg attgttgtt ttcaacttg actattcca ataactaag ggattttcg gttatttta catcatcag tctttagtc gagccggcg gtggttagt aaactgata tcgcagcta aataggagt acaggggaa gtggtgaag aaacatccg agatttttg agtaacaat acaagtgct attgttgag attcaagtt tcgaggccc attgcgctt gaactgtac agtgattgt aaggagctg gggaggttt atgctgagg gttggcggg gttacgata gctgcagga ctcattaca aaaattata ttgtag
- >TC003854 RB
atggattat tccgaagac tatgatggt tatgacgac gtttacggc cattccgtc gacgatgat tactatatt tccccaagt aatcgacag tttatctat aatcgagag aactcacga gatcaacca aaaattggc gagtttatc cgcacggaa caagacatc caagaggaa gacgaatca gaaacggcg tcagatttg tcagattta gataaggca aggttggag tcttgcatt gagcaaatt aaagccact ttgggcccc ttgtgcatt cctcgaaac gagttagta aacataata atttcaaac aattacaac atcgaacga gccactaat gcagtactg gacgacagt aagaacctg cagagtgaa ccgaaggaa aaagatgta acaaacagt tcaaaattt tttgtgcca aaattgaac ggcttcaaa gaaaacatt aagtcgccc ttcacaccc ccgcgtttt aacattcct tcactaact ggatctcaa acattcaca ataccaaaa cttaatttc cctaaagac aataccgac acaagcaaa aagtttgat agtctgtcc gatttagtc tcaaaccac ttgagcaaa acgctcaaa aatgtctca cttaactca accgagagc gttactcaa gtcacccaa aatatcaaa aatttgaac attcagcct gaaatacaa aaatcagca agtgaatat cgaattgat ctctcagtt gcgctaaaa tcgtctcca agttcccac agtttgccg aatcgaagt aaacacgat ttgactgag gaaaagttt gagattcct tttgttgac tgtgacgtc gatgtggaa agtaaaagt gcgttgaag tctgcgttt gatgctaaa gatgtgcta aatgtgtgt ctttataac gtgaaaccg gtctcgact tttggtaaa gttttgtgc cggaaatac cggagacgg aattcgatt aagtttaaa cgaagtaaa tgtgtgatt gattttacg aaaattgca atctttaat tttgccact ccttcgccc gatgataat ataattaaa cacttgaaa agttga
- >TC003854 RC
atggtggtt attggtcac gtggatgct ggaaaatcg actttgatg gggcacttg ctctatgac ctaggacag gtgaatcaa aaaacaatg cacaaatat gaacaagag agccgaaag gtgggtaaa caaagcttc atgtatgcg tgggttttg gacgaaact ggtgaggaa agaaacaga ggtataacg atggacgtc ggccgttcc caattcgaa acaaaatca aaacatgtc acattactt gacgccccc ggccacaag gatttcatc cctaatatg atatcaggg gcgggccaa gctgacgtt gctctactt gtagtggac gcaaccaga ggtgaattc gagactgga ttcgatttc ggtggtcaa acgcgagag catgcactt cttgttaga tcactagga gtcactcaa cttgctgtg gccatcaac aaattggat acggtttcg tggtccaaa gaacgattt gatgatatc tcacaaaaa ttgaaagtt tttctcaaa caagctggg tttagggaa ggcgatgtt acgtttgtt ccttgtagt gggttgact gggcaaaat ttggtcgac aaaccgacg gaaaacgag cttttgact tggtacaac gggccttgt ttattggaa gtgatcgat aatttccgt actcccgaa cgcccagtc tccaaaccg ttccgccta tccatcaat gacattttc aaagggact ggttctgga ttttgtgta tcaggtcgg gttgaaacg ggcagtttg aatgtagga gagcgggtc atggtgtgc cccagtcgc gaactctca atggtcaaa tcgctctac atcgaagat ttatcacaa acggtggtt tttgccgga gaccaagcc actgttact ttatccggt attgaaatg caaaatgtt tccattgga aacgtgctc tgcgaccct cagaacccc gtccaagtg tcggccaag ttccaggca aggattgtt gttttcaac ttgactatt ccaataact aagggattt tcggttatt ttacatcat cagtcttta gtcgagccg gcggtggtt agtaaactg atatcgcag ctaaatagg agtacaggg gaagtggtg aagaaacat ccgagattt ttgagtaac aatacaagt gctattgtt gagattcaa gtttcgagg cccattgcg cttgaactg tacagtgat tgtaaggag ctggggagg tttatgctg agggttggc ggggttacg atagctgca ggactcatt acaaaaatt atattgtag
- >TC003854 RA
cgtcaacca aaatattaa aatgtcagc tccaaagtt gtgaaaagt gcaatctgt tatttaaca cattttaaa aactttcag tgaataaaa cacacgaaa aaatgtctc gacatcgag atgttcgca atatggatt attccgaag actatgatg gttatgacg acgtttacg gccattccg tcgacgatg attactata tttccccaa gtaatcgac agtttatct ataatcgag agaactcac gagatcaac caaaaattg gcgagttta tccgcacgg aacaagaca tccaagagg aagacgaat cagaaacgg cgtcagatt tgtcagatt tagataagg caaggttgg agtcttgca ttgagcaaa ttaaagcca ctttgggcc ccttgtgca ttcctcgaa acgagttag taaacataa taatttcaa acaattaca acatcgaac gagccacta atgcagtac tggacgaca gtaagaacc tgcagagtg aaccgaagg aaaaagtgc ccagcataa aagtggtgg catcggggg gtaaaacaa ccacaaacg tcacaaaag gtttcgatc ttagccaaa ataaggatt ccattaaac taaatacca ctccacgtt cacaatcac ctgcttctg gtcgaggaa caccgatta tatctcagt ctgaaggca cttcggaag aaacgaaaa ttattaaaa gtaaagaaa ataaaattg atgttgaaa cacaataca aaaaggaga gaggcgatt cgaaagaac atctttata tggtggtta ttggtcacg tggatgctg gaaaatcga ctttgatgg ggcacttgc tctatgacc taggacagg tgaatcaaa aaacaatgc acaaatatg aacaagaga gccgaaagg tgggtaaac aaagcttca tgtatgcgt gggttttgg acgaaactg gtgaggaaa gaaacagag gtataacga tggacgtcg gccgttccc aattcgaaa caaaatcaa aacatgtca cattacttg acgcccccg gccacaagg atttcatcc ctaatatga tatcagggg cgggccaag ctgacgttg ctctacttg tagtggacg caaccagag gtgaattcg agactggat tcgatttcg gtggtcaaa cgcgagagc atgcacttc ttgttagat cactaggag tcactcaac ttgctgtgg ccatcaaca aattggata cggtttcgt ggtccaaag aacgatttg atgatatct cacaaaaat tgaaagttt ttctcaaac aagctgggt ttagggaag gcgatgtta cgtttgttc cttgtagtg ggttgactg ggcaaaatt tggtcgaca aaccgacgg aaaacgagc ttttgactt ggtacaacg ggccttgtt tattggaag tgatcgata atttccgta ctcccgaac gcccagtct ccaaaccgt tccgcctat ccatcaatg acattttca aagggactg gttctggat tttgtgtat caggtcggg ttgaaacgg gcagtttga atgtaggag agcgggtca tggtgtgcc ccagtcgcg aactctcaa tggtcaaat cgctctaca tcgaagatt tatcacaaa cggtggttt ttgccggag accaagcca ctgttactt tatccggta ttgaaatgc aaaatgttt ccattggaa acgtgctct gcgaccctc agaaccccg tccaagtgt cggccaagt tccaggcaa ggattgttg ttttcaact tgactattc caataacta agggatttt cggttattt tacatcatc agtctttag tcgagccgg cggtggtta gtaaactga tatcgcagc taaatagga gtacagggg aagtggtga agaaacatc cgagatttt tgagtaaca atacaagtg ctattgttg agattcaag tttcgaggc ccattgcgc ttgaactgt acagtgatt gtaaggagc tggggaggt ttatgctga gggttggcg gggttacga tagctgcag gactcatta caaaaatta tattgtaga gtaaggatg aataaaagt cgaaatttt at
- >TC003854 RB
cgtcaacca aaatattaa aatgtcagc tccaaagtt gtgaaaagt gcaatctgt tatttaaca cattttaaa aactttcag tgaataaaa cacacgaaa aaatgtctc gacatcgag atgttcgca atatggatt attccgaag actatgatg gttatgacg acgtttacg gccattccg tcgacgatg attactata tttccccaa gtaatcgac agtttatct ataatcgag agaactcac gagatcaac caaaaattg gcgagttta tccgcacgg aacaagaca tccaagagg aagacgaat cagaaacgg cgtcagatt tgtcagatt tagataagg caaggttgg agtcttgca ttgagcaaa ttaaagcca ctttgggcc ccttgtgca ttcctcgaa acgagttag taaacataa taatttcaa acaattaca acatcgaac gagccacta atgcagtac tggacgaca gtaagaacc tgcagagtg aaccgaagg aaaaagatg taacaaaca gttcaaaat tttttgtgc caaaattga acggcttca aagaaaaca ttaagtcgc ccttcacac ccccgcgtt ttaacattc cttcactaa ctggatctc aaacattca caataccaa aacttaatt tccctaaag acaataccg acacaagca aaaagtttg atagtctgt ccgatttag tctcaaacc acttgagca aaacgctca aaaatgtct cacttaact caaccgaga gcgttactc aagtcaccc aaaatatca aaaatttga acattcagc ctgaaatac aaaaatcag caagtgaat atcgaattg atctctcag ttgcgctaa aatcgtctc caagttccc acagtttgc cgaatcgaa gtaaacacg atttgactg aggaaaagt ttgagattc cttttgttg actgtgacg tcgatgtgg aaagtaaaa gtgcgttga agtctgcgt ttgatgcta aagatgtgc taaatgtgt gtctttata acgtgaaac cggtctcga cttttggta aagttttgt gccggaaat accggagac ggaattcga ttaagttta aacgaagta aatgtgtga ttgatttta cgaaaattg caatcttta attttgcca ctccttcgc ccgatgata atataatta aacacttga aaagttgac gacgcaagt tttcttgtg gtttttgta gtttagctg acggatcaa aatgtacat tctgtaaga ataacgtaa taaataatc attattttt agttacaaa aactcgtgt acatttata attttaatt ttacatttt catgtttta cgtatttta taaggttgg ataaaaggg aaatttata cataaagac aaataaata taatactca tac
- >TC003854 RC
ttagtaaag aaaatgaaa aaatgggaa tctaaaata ctttgcaaa aaattcacg aaacggagt aaatttcat gttgtgtta tacataaag cacacatta caatacctg aaaaattga agtttgact aagaattct tgactcgtt tgaatatgt tttcttatt gcattaaac gtaaacaat ttaatttat aaacatgtt tacaacacg tttcattat gttatgttt tcgttattt tttgccatt tattgtgat actgagaac aaatcttga agggcaact tgaatatag ttcacttat tttaattat atttacttg gagggtttt tcaaatcca gtaaaataa atttctgtc gctatatct aaagtgccc agcataaaa gtggtggca tcggggggt aaaacaacc acaaacgtc acaaaaggt ttcgatctt agccaaaat aaggattcc attaaacta aataccact ccacgttca caatcacct gcttctggt cgaggaaca ccgattata tctcagtct gaaggcact tcggaagaa acgaaaatt attaaaagt aaagaaaat aaaattgat gttgaaaca caatacaaa aaggagaga ggcgattcg aaagaacat ctttatatg gtggttatt ggtcacgtg gatgctgga aaatcgact ttgatgggg cacttgctc tatgaccta ggacaggtg aatcaaaaa acaatgcac aaatatgaa caagagagc cgaaaggtg ggtaaacaa agcttcatg tatgcgtgg gttttggac gaaactggt gaggaaaga aacagaggt ataacgatg gacgtcggc cgttcccaa ttcgaaaca aaatcaaaa catgtcaca ttacttgac gcccccggc cacaaggat ttcatccct aatatgata tcaggggcg ggccaagct gacgttgct ctacttgta gtggacgca accagaggt gaattcgag actggattc gatttcggt ggtcaaacg cgagagcat gcacttctt gttagatca ctaggagtc actcaactt gctgtggcc atcaacaaa ttggatacg gtttcgtgg tccaaagaa cgatttgat gatatctca caaaaattg aaagttttt ctcaaacaa gctgggttt agggaaggc gatgttacg tttgttcct tgtagtggg ttgactggg caaaatttg gtcgacaaa ccgacggaa aacgagctt ttgacttgg tacaacggg ccttgttta ttggaagtg atcgataat ttccgtact cccgaacgc ccagtctcc aaaccgttc cgcctatcc atcaatgac attttcaaa gggactggt tctggattt tgtgtatca ggtcgggtt gaaacgggc agtttgaat gtaggagag cgggtcatg gtgtgcccc agtcgcgaa ctctcaatg gtcaaatcg ctctacatc gaagattta tcacaaacg gtggttttt gccggagac caagccact gttacttta tccggtatt gaaatgcaa aatgtttcc attggaaac gtgctctgc gaccctcag aaccccgtc caagtgtcg gccaagttc caggcaagg attgttgtt ttcaacttg actattcca ataactaag ggattttcg gttatttta catcatcag tctttagtc gagccggcg gtggttagt aaactgata tcgcagcta aataggagt acaggggaa gtggtgaag aaacatccg agatttttg agtaacaat acaagtgct attgttgag attcaagtt tcgaggccc attgcgctt gaactgtac agtgattgt aaggagctg gggaggttt atgctgagg gttggcggg gttacgata gctgcagga ctcattaca aaaattata ttgtagagt aaggatgaa taaaagtcg aaattttat
