Status
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Gene information
General information
TC003852 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 23231537..23272466
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003852
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03634

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    lateral oviduct completenot present - Number of animals showing the phenotype: 4

    egg chamber need termshape irregular - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003852 RA
      MEVDATSST SNDSNNGNF CQNLNYYNE NLFFTTNGK 
      LDSFFNSDK SARKNGENE KVARKFNCY KKDDFSGNN 
      NVLKSKSDD VNKRVSLSV ANYNKKVDV NSENRKNLI 
      DFFNKKNMD NNSIHLNNV DSYGKKSIG FSPEQVQCM 
      CEALHQRGD IERLATFLW SLPPSELLR GNESILRAR 
      AAVAFYRGS YHELYSILE SHAFNQRWH AELQTLWFK 
      AHYNEAEKV RGRPLGAVD KYRLRKKYP LPKTIWDGE 
      ETVYCFKER SRNALKECY ARNRYPTPD EKRALAKRT 
      GLTLTQVSN WFKNRRQRD RTPQPRPEL ILGNMSLNQ 
      SGMMSAHQG SLDMVAFQA ASKLFDSNC GVASCYSDY 
      QVP
    CDS sequence
    • >TC003852 RA
      atggaggta gatgcaaca tctagtact tcaaacgat 
      tcaaacaat ggaaatttt tgtcaaaat ttgaattat 
      tataacgaa aatttgttt tttaccaca aatgggaaa 
      ctagactcg ttttttaat agtgacaag tcggcgcga 
      aagaacggt gaaaatgaa aaagtagct cgaaaattt 
      aactgttat aaaaaggac gattttagt ggtaacaac 
      aatgtgctt aaaagtaaa tcggatgat gttaataaa 
      agagtttca ttgtctgtt gctaactat aataagaaa 
      gttgatgta aactccgaa aacaggaaa aatttaatt 
      gactttttt aacaagaaa aatatggat aataacagt 
      atccatttg aacaatgtg gattcgtat ggaaaaaaa 
      tcgattgga ttttcccca gaacaggtg caatgtatg 
      tgcgaagcg ctccatcag cgaggcgac atcgaacgg 
      ctggcaaca tttctgtgg tccttgcct ccgtcggag 
      cttttgaga gggaacgaa agcattctc cgagctcgc 
      gctgctgtg gcattctac agaggctca taccacgag 
      ctgtattcc attctggag tcgcatgcg tttaatcaa 
      cgatggcat gctgaattg caaacctta tggtttaaa 
      gcgcattac aacgaagct gaaaaagtg cgcggcaga 
      ccactggga gcggtggat aagtacagg ctgaggaaa 
      aaatacccg ctccccaaa accatatgg gatggagag 
      gaaactgtt tactgcttt aaagaacgg agtcgtaat 
      gcgttgaag gagtgctat gccaggaac agatatcct 
      acgcctgat gaaaaacgg gctttggcc aaacggacc 
      ggacttact ttaacgcaa gtttcgaat tggttcaaa 
      aatcggagg cagagggat aggacgccg cagcctaga 
      ccggagctc attttgggg aacatgtcg ctcaaccaa 
      agtgggatg atgtcagct catcaagga agtctcgac 
      atggtggca tttcaagct gcttcaaaa ctcttcgac 
      tccaactgt ggtgttgct tcatgttat tcggattat 
      caagtacca taa
    mRNA sequence
    • >TC003852 RA
      aagttttaa aaacggaat ttctcttgt acgactgtc 
      ataataatt ttattgtag tcatttttt gttgtaagg 
      tttgggcaa ttctggtat ttataatta tttggaaaa 
      gaagcttaa atatttgac cagtagtta gccaaaaac 
      agagaatca aaggaccac tttttgctt cgctcattt 
      aaaattttc gctacttca tatttgttg gtcaattga 
      aaaaattac tacgcatat ctgtaaaca taaaacgta 
      atatgtatt aataagaca atttttgaa aagctgatt 
      attatagat actatgtat gtatatatc attactatg 
      taagtattt caggtacca tatcataaa ttttctatg 
      atatttatt taaaaaaat atatttgtc gattataaa 
      taacatgta agtgaagtt tattattct caattttaa 
      tgagatgtt gtaagtaga ctgagtgtt cgcttgcta 
      gaatataca atttattgt tactgtttt ttttaattt 
      aatttttta acagaaaaa ttttgcgaa aagatatca 
      aatcagatg tgatagatg gtagttaaa ttttggcaa 
      ggtaatttt ttttaattt caaacaatt tatataata 
      atgctgttt ttaggcgta tagcaagta acaagtgat 
      tcacgcaag ataacgccc ctagaagta tgcaatact 
      taatacatt gtgttgcgt acaattttt ggacgtcat 
      cttacgtaa gtcggcttg tatttcaat atttacaac 
      agtttcacc aaatgttat acgtaatca agaagcaga 
      gataaaccg gaggaagag aaaacaaag agttgatca 
      ttttttgta ctgctcttt tctcccagt tatctctgt 
      atgtgtaaa tggtaacac acgttcagg aggatgttt 
      aaactcctg tctcaagta tacttagga tgtacataa 
      tgttaggac aaaattaat gcaaattaa acgacttgt 
      tgtgaatcg tttcgacga tagtttata aaaattaaa 
      aaatgactg tgtgtttta aggattttt tatgagtga 
      tatattaat caaaaaatt ctttaataa acctcctaa 
      ctaaaccaa ctaaataaa ttaaatatt aaaccggaa 
      aagaaacaa gttaaaaca ttaagaaca gacaaccac 
      caaattaca tttattata attagtgat actttgata 
      agtaattgt gatccaagt aactattta tatgaaata 
      tttggtagt agtggttat gtacatttg agtaacaag 
      ttttgtgat gtctgattt gttcgaaat atagatcag 
      ctttagtgt cttagttaa agttttgat taacaaatt 
      tcactgtaa atactatta tgtaaaagt ttaataacg 
      taaggagac aaaattttt atggaataa tttattttt 
      attgaaggg agactaagt gaaaaattt attaaaatt 
      aaattgttc cataaataa ttttgggaa attgccatg 
      gtctgtctt ccataaaat aaaataaac taaatgtgt 
      agtttatgt gcttgtgta cattgacac agtaatgtt 
      aacataaaa ttatccaat tattgatat tataagtca 
      gaagtgtaa aatttgcaa acgtgttgt gttaatttt 
      ttttgaaaa agtgcaatt aaaatgact tgaatttgg 
      tgtaccatg gaggtagat gcaacatct agtacttca 
      aacgattca aacaatgga aatttttgt caaaatttg 
      aattattat aacgaaaat ttgtttttt accacaaat 
      gggaaacta gactcgttt tttaatagt gacaagtcg 
      gcgcgaaag aacggtgaa aatgaaaaa gtagctcga 
      aaatttaac tgttataaa aaggacgat tttagtggt 
      aacaacaat gtgcttaaa agtaaatcg gatgatgtt 
      aataaaaga gtttcattg tctgttgct aactataat 
      aagaaagtt gatgtaaac tccgaaaac aggaaaaat 
      ttaattgac ttttttaac aagaaaaat atggataat 
      aacagtatc catttgaac aatgtggat tcgtatgga 
      aaaaaatcg attggattt tccccagaa caggtgcaa 
      tgtatgtgc gaagcgctc catcagcga ggcgacatc 
      gaacggctg gcaacattt ctgtggtcc ttgcctccg 
      tcggagctt ttgagaggg aacgaaagc attctccga 
      gctcgcgct gctgtggca ttctacaga ggctcatac 
      cacgagctg tattccatt ctggagtcg catgcgttt 
      aatcaacga tggcatgct gaattgcaa accttatgg 
      tttaaagcg cattacaac gaagctgaa aaagtgcgc 
      ggcagacca ctgggagcg gtggataag tacaggctg 
      aggaaaaaa tacccgctc cccaaaacc atatgggat 
      ggagaggaa actgtttac tgctttaaa gaacggagt 
      cgtaatgcg ttgaaggag tgctatgcc aggaacaga 
      tatcctacg cctgatgaa aaacgggct ttggccaaa 
      cggaccgga cttacttta acgcaagtt tcgaattgg 
      ttcaaaaat cggaggcag agggatagg acgccgcag 
      cctagaccg gagctcatt ttggggaac atgtcgctc 
      aaccaaagt gggatgatg tcagctcat caaggaagt 
      ctcgacatg gtggcattt caagctgct tcaaaactc 
      ttcgactcc aactgtggt gttgcttca tgttattcg 
      gattatcaa gtaccataa atttagaat ctggaattg 
      ttacgtaat gattgacgc caaaaacac acttgttac 
      ctttgtaat ggaatttat gtaaatttt tgtatataa 
      tataacgat taaatagtt
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