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Gene information
General information
TC003849 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 23117693..23128999
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003849
    iBeetle screen iB_03631

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003849 RA
      MSGGGNKKK PTKTQGWEE AGSEFYQES YPTDAELDA 
LQRDPNPKH LATLLPSKQ TRAAATIRI RTNDNRTIR 
RQTASRSRR DSTVHRRAS TAGDVQVAM LPDLSENLS 
NEQTTWEEI MQIKGMPVP MSQKKEMKA KILNEPNLR 
LQGYEQLNW KRRKIWRQF ISRLKEMHT KIELWRSAL 
KRIEGHFGT GVVAYFIFL KYLLFLNLF IFTVIFLFI 
ILPTILLVN SDTQNPPNS SNVVFDLIQ GTGFMEHSL 
LFYGFYPNE TFDYLVNES VMYYNLPLA YVCITVVYF 
ATSLVSMVK SAANGFKER LIEGEGQFY QYCNFIFGG 
WDFCIHNEK SAAIKHKAI YNEIKACLA AERLEEMKQ 
NRTKGEHTK LIIVRILVN VIVLAVLGA CGTAIFFVF 
QEFRNESSD DYFEGLLFQ FLPSITIVC FNILVPFLF 
KYLIQFERY SPLVVVRFT LFRTVLLRL ASIFTLYIT 
LGSVLTSSE KPNCWETFA GQQIYKLVI TDFATHVIL 
TFLVNFPRA FIARHVENK FIKLVGEQS FDLPKHVLD 
VVYLQTLCW LGCFYAPLL SFIASLIFF FMFYIKKFV 
CLVNSNPSA TVYRASRSN SMFMIVLLV SFVSVVAPL 
AYSFSEVEP SRGCGPFRD LPSVWSRVE FTFYSTPDW 
VQSVVAFLS TAGFAVPVF LLLFLLLYY FRAVNAANR 
QMVVVLKNQ LVLEGHDKQ FLLERLSKF IKQQQEHQK 
RLRHVDVLQ DGDTNISSN
    CDS sequence
    • >TC003849 RA
      atgtcagga ggcggaaat aagaaaaaa cccaccaag 
acccaaggt tgggaagag gcagggtct gagttttat 
caagaaagt taccccact gatgctgaa ttggacgcc 
ttgcaaaga gacccaaat cctaaacat ttggccacg 
ttgctgcca agcaagcaa acaagggca gctgcgaca 
attaggatt cgtacaaat gataacagg acaattaga 
agacaaaca gcttcgcgt tcgcgaaga gactcaact 
gtgcatagg agggcgtca actgcagga gatgtgcaa 
gtggctatg cttccagat ttatcggaa aacttgtct 
aatgaacaa accacatgg gaagagatt atgcagatc 
aagggaatg ccggtaccc atgtcgcag aagaaggaa 
atgaaagca aaaattcta aacgaaccg aatttgcgt 
ttacaaggc tacgaacaa ctcaactgg aaacgcagg 
aagatctgg cgccagttc atctcccgc ttgaaggaa 
atgcacact aaaatcgaa ctttggcga agcgcctta 
aaacgcatc gaaggccac ttcggcacc ggcgtcgtc 
gcctacttc attttcctc aagtacctc ctcttccta 
aacttattc atatttacg gtcattttc ctcttcata 
attctacca acaatactt ctagtcaat tccgacaca 
caaaatcca cccaacagc tcgaacgtc gtgttcgac 
ctcatccaa ggcaccggc ttcatggag cacagtcta 
ctgttttac ggtttttac ccgaacgaa acgttcgat 
tatctcgtc aacgagagc gtgatgtac tataatctt 
ccattagcg tacgtttgt atcacagtt gtatatttc 
gcaacgtcg ctcgtttcc atggtcaag tcggcggcc 
aacggtttt aaagaacgg ttgattgaa ggcgagggc 
cagttttac caatactgt aattttatt ttcggaggg 
tgggacttt tgcatccat aatgaaaaa tcagccgca 
attaaacac aaagccatc tataacgaa atcaaggcg 
tgtcttgcg gccgaacgg ttggaggaa atgaaacag 
aatcggacc aaaggcgaa cacaccaaa ctaatcatt 
gttcgaatc cttgtaaat gtgatagtg ttggcggtt 
ttgggggcg tgtggcact gcgattttt ttcgttttt 
caagaattt cggaacgag tccagcgat gattatttc 
gaagggctt ttgttccag tttttgccc tcgattacc 
atcgtctgt ttcaatatt ttggtccct tttttgttc 
aaatatttg atacagttc gagcgctac agcccactt 
gttgtggtg cgttttacg ctgtttcgg accgttttg 
ctgcgtctt gcctcgatt tttacactt tatatcaca 
cttggtagt gtacttaca agtagcgaa aaacctaac 
tgttgggag actttcgcc ggccaacaa atctacaag 
ttggttata accgatttt gcgacgcac gtcatcctc 
acgttcttg gtcaatttc ccgcgcgct ttcatcgca 
cgacacgtc gaaaataag tttataaag ctagtcggg 
gagcaaagt ttcgacttg ccgaagcat gtgcttgac 
gtcgtttat ttgcaaact ttgtgttgg ttggggtgt 
ttctacgcc cccttgctc tctttcatc gcctcgctg 
atttttttc ttcatgttt tatatcaaa aagttcgtt 
tgtttggtt aacagtaac ccgagtgcg accgtttat 
cgcgcttcg cgctccaat tcgatgttt atgattgtg 
ttgctggtg tctttcgtg tcggttgtg gcccccttg 
gcgtattct ttttcggag gtggagccc tcgaggggc 
tgcggtccc ttccgggac ttgccttcc gtctggagt 
cgggtcgag tttaccttt tattcgact cctgattgg 
gtgcagagt gtggtcgcg tttttgagt actgccggg 
tttgcggtg cccgttttt ctgctcctg tttcttttg 
ttgtactat tttcgggcc gtgaatgcg gcgaatagg 
cagatggtg gtcgtgttg aagaatcag ctggttttg 
gaggggcat gataaacaa tttttattg gaaagactg 
agtaagttt attaagcag caacaggag catcagaaa 
aggttgagg catgttgat gttttgcaa gacggtgat 
actaatatt tcgagcaat taa
    mRNA sequence
    • >TC003849 RA
      tacccttaa taaaggtca atgacataa ggcgtaatt 
ttgacagat gtcaaatag attggttat ttttttcac 
gccaacagc gatgagttt tgtgctaat tgtttacaa 
ccaccgaat taattcaaa aaattaaac aatgagcaa 
ggaaagatt agatttcat cgattgcac caggaaaac 
gagctcaca ctaattcac acgttgttg tttatctaa 
tctgtgtag gatgaagtt ataaattta cagatttgc 
tcgcgtccg cgtttccaa aatgtcagg aggcggaaa 
taagaaaaa acccaccaa gacccaagg ttgggaaga 
ggcagggtc tgagtttta tcaagaaag ttaccccac 
tgatgctga attggacgc cttgcaaag agacccaaa 
tcctaaaca tttggccac gttgctgcc aagcaagca 
aacaagggc agctgcgac aattaggat tcgtacaaa 
tgataacag gacaattag aagacaaac agcttcgcg 
ttcgcgaag agactcaac tgtgcatag gagggcgtc 
aactgcagg agatgtgca agtggctat gcttccaga 
tttatcgga aaacttgtc taatgaaca aaccacatg 
ggaagagat tatgcagat caagggaat gccggtacc 
catgtcgca gaagaagga aatgaaagc aaaaattct 
aaacgaacc gaatttgcg tttacaagg ctacgaaca 
actcaactg gaaacgcag gaagatctg gcgccagtt 
catctcccg cttgaagga aatgcacac taaaatcga 
actttggcg aagcgcctt aaaacgcat cgaaggcca 
cttcggcac cggcgtcgt cgcctactt cattttcct 
caagtacct cctcttcct aaacttatt catatttac 
ggtcatttt cctcttcat aattctacc aacaatact 
tctagtcaa ttccgacac acaaaatcc acccaacag 
ctcgaacgt cgtgttcga cctcatcca aggcaccgg 
cttcatgga gcacagtct actgtttta cggttttta 
cccgaacga aacgttcga ttatctcgt caacgagag 
cgtgatgta ctataatct tccattagc gtacgtttg 
tatcacagt tgtatattt cgcaacgtc gctcgtttc 
catggtcaa gtcggcggc caacggttt taaagaacg 
gttgattga aggcgaggg ccagtttta ccaatactg 
taattttat tttcggagg gtgggactt ttgcatcca 
taatgaaaa atcagccgc aattaaaca caaagccat 
ctataacga aatcaaggc gtgtcttgc ggccgaacg 
gttggagga aatgaaaca gaatcggac caaaggcga 
acacaccaa actaatcat tgttcgaat ccttgtaaa 
tgtgatagt gttggcggt tttgggggc gtgtggcac 
tgcgatttt tttcgtttt tcaagaatt tcggaacga 
gtccagcga tgattattt cgaagggct tttgttcca 
gtttttgcc ctcgattac catcgtctg tttcaatat 
tttggtccc ttttttgtt caaatattt gatacagtt 
cgagcgcta cagcccact tgttgtggt gcgttttac 
gctgtttcg gaccgtttt gctgcgtct tgcctcgat 
ttttacact ttatatcac acttggtag tgtacttac 
aagtagcga aaaacctaa ctgttggga gactttcgc 
cggccaaca aatctacaa gttggttat aaccgattt 
tgcgacgca cgtcatcct cacgttctt ggtcaattt 
cccgcgcgc tttcatcgc acgacacgt cgaaaataa 
gtttataaa gctagtcgg ggagcaaag tttcgactt 
gccgaagca tgtgcttga cgtcgttta tttgcaaac 
tttgtgttg gttggggtg tttctacgc ccccttgct 
ctctttcat cgcctcgct gattttttt cttcatgtt 
ttatatcaa aaagttcgt ttgtttggt taacagtaa 
cccgagtgc gaccgttta tcgcgcttc gcgctccaa 
ttcgatgtt tatgattgt gttgctggt gtctttcgt 
gtcggttgt ggccccctt ggcgtattc tttttcgga 
ggtggagcc ctcgagggg ctgcggtcc cttccggga 
cttgccttc cgtctggag tcgggtcga gtttacctt 
ttattcgac tcctgattg ggtgcagag tgtggtcgc 
gtttttgag tactgccgg gtttgcggt gcccgtttt 
tctgctcct gtttctttt gttgtacta ttttcgggc 
cgtgaatgc ggcgaatag gcagatggt ggtcgtgtt 
gaagaatca gctggtttt ggaggggca tgataaaca 
atttttatt ggaaagact gagtaagtt tattaagca 
gcaacagga gcatcagaa aaggttgag gcatgttga 
tgttttgca agacggtga tactaatat ttcgagcaa 
ttaaggggc gtattctag tcagattta tttattatt 
gtaatggac ttgatattt taggctttc gtgatgatt 
tttaataaa gtacggggt actaaatta caaaacacc 
gcttgagct ctaaaaaat gtaatcaaa atctatcgg 
catttttaa tcaagttat tgtattatt tttattttg 
ttattattt attatactg gggtgtaac gaaatgtgg 
ggacaaatg ggtacaaga gatttccca ggttcctgt 
ttaaaagtt agagggcgc caaagttca atagttaca 
gtgaaactc ggttataac gaacactca aggaacttt 
acaattggt acagtcact atcaaaaag aatgacacc 
cctaccaac tggtagttt atgaactag ccaaacgta 
cacattaat cgcattata tcgaaaaga tttctagct 
actgtttta gcggtgtca ttctttgtg gtcgtgact 
gtacgttat aaccgatcg ttcgttcta accgaacat 
catctttat tgagtaatt tctaagtta aatgaattc 
gaaagcttg gaatgatgc acaaaataa agtattaat 
ctaatttat taagaaaga tacatttac atacatggt 
gtcccctat tgaccttta aatgttgaa tttttttga 
gctgcaata caaacacgc atagttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY