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Gene information
General information
TC003849 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid23: 632677..643983
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003849
    iBeetle screen iB_03631
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003849 RA
      MSGGGNKKK PTKTQGWEE AGSEFYQES YPTDAELDA 
      LQRDPNPKH LATLLPSKQ TRAAATIRI RTNDNRTIR 
      RQTASRSRR DSTVHRRAS TAGDVQVAM LPDLSENLS 
      NEQTTWEEI MQIKGMPVP MSQKKEMKA KILNEPNLR 
      LQGYEQLNW KRRKIWRQF ISRLKEMHT KIELWRSAL 
      KRIEGHFGT GVVAYFIFL KYLLFLNLF IFTVIFLFI 
      ILPTILLVN SDTQNPPNS SNVVFDLIQ GTGFMEHSL 
      LFYGFYPNE TFDYLVNES VMYYNLPLA YVCITVVYF 
      ATSLVSMVK SAANGFKER LIEGEGQFY QYCNFIFGG 
      WDFCIHNEK SAAIKHKAI YNEIKACLA AERLEEMKQ 
      NRTKGEHTK LIIVRILVN VIVLAVLGA CGTAIFFVF 
      QEFRNESSD DYFEGLLFQ FLPSITIVC FNILVPFLF 
      KYLIQFERY SPLVVVRFT LFRTVLLRL ASIFTLYIT 
      LGSVLTSSE KPNCWETFA GQQIYKLVI TDFATHVIL 
      TFLVNFPRA FIARHVENK FIKLVGEQS FDLPKHVLD 
      VVYLQTLCW LGCFYAPLL SFIASLIFF FMFYIKKFV 
      CLVNSNPSA TVYRASRSN SMFMIVLLV SFVSVVAPL 
      AYSFSEVEP SRGCGPFRD LPSVWSRVE FTFYSTPDW 
      VQSVVAFLS TAGFAVPVF LLLFLLLYY FRAVNAANR 
      QMVVVLKNQ LVLEGHDKQ FLLERLSKF IKQQQEHQK 
      RLRHVDVLQ DGDTNISSN
    CDS sequence
    • >TC003849 RA
      atgtcagga ggcggaaat aagaaaaaa cccaccaag 
      acccaaggt tgggaagag gcagggtct gagttttat 
      caagaaagt taccccact gatgctgaa ttggacgcc 
      ttgcaaaga gacccaaat cctaaacat ttggccacg 
      ttgctgcca agcaagcaa acaagggca gctgcgaca 
      attaggatt cgtacaaat gataacagg acaattaga 
      agacaaaca gcttcgcgt tcgcgaaga gactcaact 
      gtgcatagg agggcgtca actgcagga gatgtgcaa 
      gtggctatg cttccagat ttatcggaa aacttgtct 
      aatgaacaa accacatgg gaagagatt atgcagatc 
      aagggaatg ccggtaccc atgtcgcag aagaaggaa 
      atgaaagca aaaattcta aacgaaccg aatttgcgt 
      ttacaaggc tacgaacaa ctcaactgg aaacgcagg 
      aagatctgg cgccagttc atctcccgc ttgaaggaa 
      atgcacact aaaatcgaa ctttggcga agcgcctta 
      aaacgcatc gaaggccac ttcggcacc ggcgtcgtc 
      gcctacttc attttcctc aagtacctc ctcttccta 
      aacttattc atatttacg gtcattttc ctcttcata 
      attctacca acaatactt ctagtcaat tccgacaca 
      caaaatcca cccaacagc tcgaacgtc gtgttcgac 
      ctcatccaa ggcaccggc ttcatggag cacagtcta 
      ctgttttac ggtttttac ccgaacgaa acgttcgat 
      tatctcgtc aacgagagc gtgatgtac tataatctt 
      ccattagcg tacgtttgt atcacagtt gtatatttc 
      gcaacgtcg ctcgtttcc atggtcaag tcggcggcc 
      aacggtttt aaagaacgg ttgattgaa ggcgagggc 
      cagttttac caatactgt aattttatt ttcggaggg 
      tgggacttt tgcatccat aatgaaaaa tcagccgca 
      attaaacac aaagccatc tataacgaa atcaaggcg 
      tgtcttgcg gccgaacgg ttggaggaa atgaaacag 
      aatcggacc aaaggcgaa cacaccaaa ctaatcatt 
      gttcgaatc cttgtaaat gtgatagtg ttggcggtt 
      ttgggggcg tgtggcact gcgattttt ttcgttttt 
      caagaattt cggaacgag tccagcgat gattatttc 
      gaagggctt ttgttccag tttttgccc tcgattacc 
      atcgtctgt ttcaatatt ttggtccct tttttgttc 
      aaatatttg atacagttc gagcgctac agcccactt 
      gttgtggtg cgttttacg ctgtttcgg accgttttg 
      ctgcgtctt gcctcgatt tttacactt tatatcaca 
      cttggtagt gtacttaca agtagcgaa aaacctaac 
      tgttgggag actttcgcc ggccaacaa atctacaag 
      ttggttata accgatttt gcgacgcac gtcatcctc 
      acgttcttg gtcaatttc ccgcgcgct ttcatcgca 
      cgacacgtc gaaaataag tttataaag ctagtcggg 
      gagcaaagt ttcgacttg ccgaagcat gtgcttgac 
      gtcgtttat ttgcaaact ttgtgttgg ttggggtgt 
      ttctacgcc cccttgctc tctttcatc gcctcgctg 
      atttttttc ttcatgttt tatatcaaa aagttcgtt 
      tgtttggtt aacagtaac ccgagtgcg accgtttat 
      cgcgcttcg cgctccaat tcgatgttt atgattgtg 
      ttgctggtg tctttcgtg tcggttgtg gcccccttg 
      gcgtattct ttttcggag gtggagccc tcgaggggc 
      tgcggtccc ttccgggac ttgccttcc gtctggagt 
      cgggtcgag tttaccttt tattcgact cctgattgg 
      gtgcagagt gtggtcgcg tttttgagt actgccggg 
      tttgcggtg cccgttttt ctgctcctg tttcttttg 
      ttgtactat tttcgggcc gtgaatgcg gcgaatagg 
      cagatggtg gtcgtgttg aagaatcag ctggttttg 
      gaggggcat gataaacaa tttttattg gaaagactg 
      agtaagttt attaagcag caacaggag catcagaaa 
      aggttgagg catgttgat gttttgcaa gacggtgat 
      actaatatt tcgagcaat taa
    mRNA sequence
    • >TC003849 RA
      tacccttaa taaaggtca atgacataa ggcgtaatt 
      ttgacagat gtcaaatag attggttat ttttttcac 
      gccaacagc gatgagttt tgtgctaat tgtttacaa 
      ccaccgaat taattcaaa aaattaaac aatgagcaa 
      ggaaagatt agatttcat cgattgcac caggaaaac 
      gagctcaca ctaattcac acgttgttg tttatctaa 
      tctgtgtag gatgaagtt ataaattta cagatttgc 
      tcgcgtccg cgtttccaa aatgtcagg aggcggaaa 
      taagaaaaa acccaccaa gacccaagg ttgggaaga 
      ggcagggtc tgagtttta tcaagaaag ttaccccac 
      tgatgctga attggacgc cttgcaaag agacccaaa 
      tcctaaaca tttggccac gttgctgcc aagcaagca 
      aacaagggc agctgcgac aattaggat tcgtacaaa 
      tgataacag gacaattag aagacaaac agcttcgcg 
      ttcgcgaag agactcaac tgtgcatag gagggcgtc 
      aactgcagg agatgtgca agtggctat gcttccaga 
      tttatcgga aaacttgtc taatgaaca aaccacatg 
      ggaagagat tatgcagat caagggaat gccggtacc 
      catgtcgca gaagaagga aatgaaagc aaaaattct 
      aaacgaacc gaatttgcg tttacaagg ctacgaaca 
      actcaactg gaaacgcag gaagatctg gcgccagtt 
      catctcccg cttgaagga aatgcacac taaaatcga 
      actttggcg aagcgcctt aaaacgcat cgaaggcca 
      cttcggcac cggcgtcgt cgcctactt cattttcct 
      caagtacct cctcttcct aaacttatt catatttac 
      ggtcatttt cctcttcat aattctacc aacaatact 
      tctagtcaa ttccgacac acaaaatcc acccaacag 
      ctcgaacgt cgtgttcga cctcatcca aggcaccgg 
      cttcatgga gcacagtct actgtttta cggttttta 
      cccgaacga aacgttcga ttatctcgt caacgagag 
      cgtgatgta ctataatct tccattagc gtacgtttg 
      tatcacagt tgtatattt cgcaacgtc gctcgtttc 
      catggtcaa gtcggcggc caacggttt taaagaacg 
      gttgattga aggcgaggg ccagtttta ccaatactg 
      taattttat tttcggagg gtgggactt ttgcatcca 
      taatgaaaa atcagccgc aattaaaca caaagccat 
      ctataacga aatcaaggc gtgtcttgc ggccgaacg 
      gttggagga aatgaaaca gaatcggac caaaggcga 
      acacaccaa actaatcat tgttcgaat ccttgtaaa 
      tgtgatagt gttggcggt tttgggggc gtgtggcac 
      tgcgatttt tttcgtttt tcaagaatt tcggaacga 
      gtccagcga tgattattt cgaagggct tttgttcca 
      gtttttgcc ctcgattac catcgtctg tttcaatat 
      tttggtccc ttttttgtt caaatattt gatacagtt 
      cgagcgcta cagcccact tgttgtggt gcgttttac 
      gctgtttcg gaccgtttt gctgcgtct tgcctcgat 
      ttttacact ttatatcac acttggtag tgtacttac 
      aagtagcga aaaacctaa ctgttggga gactttcgc 
      cggccaaca aatctacaa gttggttat aaccgattt 
      tgcgacgca cgtcatcct cacgttctt ggtcaattt 
      cccgcgcgc tttcatcgc acgacacgt cgaaaataa 
      gtttataaa gctagtcgg ggagcaaag tttcgactt 
      gccgaagca tgtgcttga cgtcgttta tttgcaaac 
      tttgtgttg gttggggtg tttctacgc ccccttgct 
      ctctttcat cgcctcgct gattttttt cttcatgtt 
      ttatatcaa aaagttcgt ttgtttggt taacagtaa 
      cccgagtgc gaccgttta tcgcgcttc gcgctccaa 
      ttcgatgtt tatgattgt gttgctggt gtctttcgt 
      gtcggttgt ggccccctt ggcgtattc tttttcgga 
      ggtggagcc ctcgagggg ctgcggtcc cttccggga 
      cttgccttc cgtctggag tcgggtcga gtttacctt 
      ttattcgac tcctgattg ggtgcagag tgtggtcgc 
      gtttttgag tactgccgg gtttgcggt gcccgtttt 
      tctgctcct gtttctttt gttgtacta ttttcgggc 
      cgtgaatgc ggcgaatag gcagatggt ggtcgtgtt 
      gaagaatca gctggtttt ggaggggca tgataaaca 
      atttttatt ggaaagact gagtaagtt tattaagca 
      gcaacagga gcatcagaa aaggttgag gcatgttga 
      tgttttgca agacggtga tactaatat ttcgagcaa 
      ttaaggggc gtattctag tcagattta tttattatt 
      gtaatggac ttgatattt taggctttc gtgatgatt 
      tttaataaa gtacggggt actaaatta caaaacacc 
      gcttgagct ctaaaaaat gtaatcaaa atctatcgg 
      catttttaa tcaagttat tgtattatt tttattttg 
      ttattattt attatactg gggtgtaac gaaatgtgg 
      ggacaaatg ggtacaaga gatttccca ggttcctgt 
      ttaaaagtt agagggcgc caaagttca atagttaca 
      gtgaaactc ggttataac gaacactca aggaacttt 
      acaattggt acagtcact atcaaaaag aatgacacc 
      cctaccaac tggtagttt atgaactag ccaaacgta 
      cacattaat cgcattata tcgaaaaga tttctagct 
      actgtttta gcggtgtca ttctttgtg gtcgtgact 
      gtacgttat aaccgatcg ttcgttcta accgaacat 
      catctttat tgagtaatt tctaagtta aatgaattc 
      gaaagcttg gaatgatgc acaaaataa agtattaat 
      ctaatttat taagaaaga tacatttac atacatggt 
      gtcccctat tgaccttta aatgttgaa tttttttga 
      gctgcaata caaacacgc atagttt