| General information | |||
| TC003849 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
scaffold_seqid23: 632677..643983 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC003849 | ||
| General information | ||||
| iB_03631 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (16 dpi)
- >TC003849 RA
MSGGGNKKK PTKTQGWEE AGSEFYQES YPTDAELDA LQRDPNPKH LATLLPSKQ TRAAATIRI RTNDNRTIR RQTASRSRR DSTVHRRAS TAGDVQVAM LPDLSENLS NEQTTWEEI MQIKGMPVP MSQKKEMKA KILNEPNLR LQGYEQLNW KRRKIWRQF ISRLKEMHT KIELWRSAL KRIEGHFGT GVVAYFIFL KYLLFLNLF IFTVIFLFI ILPTILLVN SDTQNPPNS SNVVFDLIQ GTGFMEHSL LFYGFYPNE TFDYLVNES VMYYNLPLA YVCITVVYF ATSLVSMVK SAANGFKER LIEGEGQFY QYCNFIFGG WDFCIHNEK SAAIKHKAI YNEIKACLA AERLEEMKQ NRTKGEHTK LIIVRILVN VIVLAVLGA CGTAIFFVF QEFRNESSD DYFEGLLFQ FLPSITIVC FNILVPFLF KYLIQFERY SPLVVVRFT LFRTVLLRL ASIFTLYIT LGSVLTSSE KPNCWETFA GQQIYKLVI TDFATHVIL TFLVNFPRA FIARHVENK FIKLVGEQS FDLPKHVLD VVYLQTLCW LGCFYAPLL SFIASLIFF FMFYIKKFV CLVNSNPSA TVYRASRSN SMFMIVLLV SFVSVVAPL AYSFSEVEP SRGCGPFRD LPSVWSRVE FTFYSTPDW VQSVVAFLS TAGFAVPVF LLLFLLLYY FRAVNAANR QMVVVLKNQ LVLEGHDKQ FLLERLSKF IKQQQEHQK RLRHVDVLQ DGDTNISSN
- >TC003849 RA
atgtcagga ggcggaaat aagaaaaaa cccaccaag acccaaggt tgggaagag gcagggtct gagttttat caagaaagt taccccact gatgctgaa ttggacgcc ttgcaaaga gacccaaat cctaaacat ttggccacg ttgctgcca agcaagcaa acaagggca gctgcgaca attaggatt cgtacaaat gataacagg acaattaga agacaaaca gcttcgcgt tcgcgaaga gactcaact gtgcatagg agggcgtca actgcagga gatgtgcaa gtggctatg cttccagat ttatcggaa aacttgtct aatgaacaa accacatgg gaagagatt atgcagatc aagggaatg ccggtaccc atgtcgcag aagaaggaa atgaaagca aaaattcta aacgaaccg aatttgcgt ttacaaggc tacgaacaa ctcaactgg aaacgcagg aagatctgg cgccagttc atctcccgc ttgaaggaa atgcacact aaaatcgaa ctttggcga agcgcctta aaacgcatc gaaggccac ttcggcacc ggcgtcgtc gcctacttc attttcctc aagtacctc ctcttccta aacttattc atatttacg gtcattttc ctcttcata attctacca acaatactt ctagtcaat tccgacaca caaaatcca cccaacagc tcgaacgtc gtgttcgac ctcatccaa ggcaccggc ttcatggag cacagtcta ctgttttac ggtttttac ccgaacgaa acgttcgat tatctcgtc aacgagagc gtgatgtac tataatctt ccattagcg tacgtttgt atcacagtt gtatatttc gcaacgtcg ctcgtttcc atggtcaag tcggcggcc aacggtttt aaagaacgg ttgattgaa ggcgagggc cagttttac caatactgt aattttatt ttcggaggg tgggacttt tgcatccat aatgaaaaa tcagccgca attaaacac aaagccatc tataacgaa atcaaggcg tgtcttgcg gccgaacgg ttggaggaa atgaaacag aatcggacc aaaggcgaa cacaccaaa ctaatcatt gttcgaatc cttgtaaat gtgatagtg ttggcggtt ttgggggcg tgtggcact gcgattttt ttcgttttt caagaattt cggaacgag tccagcgat gattatttc gaagggctt ttgttccag tttttgccc tcgattacc atcgtctgt ttcaatatt ttggtccct tttttgttc aaatatttg atacagttc gagcgctac agcccactt gttgtggtg cgttttacg ctgtttcgg accgttttg ctgcgtctt gcctcgatt tttacactt tatatcaca cttggtagt gtacttaca agtagcgaa aaacctaac tgttgggag actttcgcc ggccaacaa atctacaag ttggttata accgatttt gcgacgcac gtcatcctc acgttcttg gtcaatttc ccgcgcgct ttcatcgca cgacacgtc gaaaataag tttataaag ctagtcggg gagcaaagt ttcgacttg ccgaagcat gtgcttgac gtcgtttat ttgcaaact ttgtgttgg ttggggtgt ttctacgcc cccttgctc tctttcatc gcctcgctg atttttttc ttcatgttt tatatcaaa aagttcgtt tgtttggtt aacagtaac ccgagtgcg accgtttat cgcgcttcg cgctccaat tcgatgttt atgattgtg ttgctggtg tctttcgtg tcggttgtg gcccccttg gcgtattct ttttcggag gtggagccc tcgaggggc tgcggtccc ttccgggac ttgccttcc gtctggagt cgggtcgag tttaccttt tattcgact cctgattgg gtgcagagt gtggtcgcg tttttgagt actgccggg tttgcggtg cccgttttt ctgctcctg tttcttttg ttgtactat tttcgggcc gtgaatgcg gcgaatagg cagatggtg gtcgtgttg aagaatcag ctggttttg gaggggcat gataaacaa tttttattg gaaagactg agtaagttt attaagcag caacaggag catcagaaa aggttgagg catgttgat gttttgcaa gacggtgat actaatatt tcgagcaat taa
- >TC003849 RA
tacccttaa taaaggtca atgacataa ggcgtaatt ttgacagat gtcaaatag attggttat ttttttcac gccaacagc gatgagttt tgtgctaat tgtttacaa ccaccgaat taattcaaa aaattaaac aatgagcaa ggaaagatt agatttcat cgattgcac caggaaaac gagctcaca ctaattcac acgttgttg tttatctaa tctgtgtag gatgaagtt ataaattta cagatttgc tcgcgtccg cgtttccaa aatgtcagg aggcggaaa taagaaaaa acccaccaa gacccaagg ttgggaaga ggcagggtc tgagtttta tcaagaaag ttaccccac tgatgctga attggacgc cttgcaaag agacccaaa tcctaaaca tttggccac gttgctgcc aagcaagca aacaagggc agctgcgac aattaggat tcgtacaaa tgataacag gacaattag aagacaaac agcttcgcg ttcgcgaag agactcaac tgtgcatag gagggcgtc aactgcagg agatgtgca agtggctat gcttccaga tttatcgga aaacttgtc taatgaaca aaccacatg ggaagagat tatgcagat caagggaat gccggtacc catgtcgca gaagaagga aatgaaagc aaaaattct aaacgaacc gaatttgcg tttacaagg ctacgaaca actcaactg gaaacgcag gaagatctg gcgccagtt catctcccg cttgaagga aatgcacac taaaatcga actttggcg aagcgcctt aaaacgcat cgaaggcca cttcggcac cggcgtcgt cgcctactt cattttcct caagtacct cctcttcct aaacttatt catatttac ggtcatttt cctcttcat aattctacc aacaatact tctagtcaa ttccgacac acaaaatcc acccaacag ctcgaacgt cgtgttcga cctcatcca aggcaccgg cttcatgga gcacagtct actgtttta cggttttta cccgaacga aacgttcga ttatctcgt caacgagag cgtgatgta ctataatct tccattagc gtacgtttg tatcacagt tgtatattt cgcaacgtc gctcgtttc catggtcaa gtcggcggc caacggttt taaagaacg gttgattga aggcgaggg ccagtttta ccaatactg taattttat tttcggagg gtgggactt ttgcatcca taatgaaaa atcagccgc aattaaaca caaagccat ctataacga aatcaaggc gtgtcttgc ggccgaacg gttggagga aatgaaaca gaatcggac caaaggcga acacaccaa actaatcat tgttcgaat ccttgtaaa tgtgatagt gttggcggt tttgggggc gtgtggcac tgcgatttt tttcgtttt tcaagaatt tcggaacga gtccagcga tgattattt cgaagggct tttgttcca gtttttgcc ctcgattac catcgtctg tttcaatat tttggtccc ttttttgtt caaatattt gatacagtt cgagcgcta cagcccact tgttgtggt gcgttttac gctgtttcg gaccgtttt gctgcgtct tgcctcgat ttttacact ttatatcac acttggtag tgtacttac aagtagcga aaaacctaa ctgttggga gactttcgc cggccaaca aatctacaa gttggttat aaccgattt tgcgacgca cgtcatcct cacgttctt ggtcaattt cccgcgcgc tttcatcgc acgacacgt cgaaaataa gtttataaa gctagtcgg ggagcaaag tttcgactt gccgaagca tgtgcttga cgtcgttta tttgcaaac tttgtgttg gttggggtg tttctacgc ccccttgct ctctttcat cgcctcgct gattttttt cttcatgtt ttatatcaa aaagttcgt ttgtttggt taacagtaa cccgagtgc gaccgttta tcgcgcttc gcgctccaa ttcgatgtt tatgattgt gttgctggt gtctttcgt gtcggttgt ggccccctt ggcgtattc tttttcgga ggtggagcc ctcgagggg ctgcggtcc cttccggga cttgccttc cgtctggag tcgggtcga gtttacctt ttattcgac tcctgattg ggtgcagag tgtggtcgc gtttttgag tactgccgg gtttgcggt gcccgtttt tctgctcct gtttctttt gttgtacta ttttcgggc cgtgaatgc ggcgaatag gcagatggt ggtcgtgtt gaagaatca gctggtttt ggaggggca tgataaaca atttttatt ggaaagact gagtaagtt tattaagca gcaacagga gcatcagaa aaggttgag gcatgttga tgttttgca agacggtga tactaatat ttcgagcaa ttaaggggc gtattctag tcagattta tttattatt gtaatggac ttgatattt taggctttc gtgatgatt tttaataaa gtacggggt actaaatta caaaacacc gcttgagct ctaaaaaat gtaatcaaa atctatcgg catttttaa tcaagttat tgtattatt tttattttg ttattattt attatactg gggtgtaac gaaatgtgg ggacaaatg ggtacaaga gatttccca ggttcctgt ttaaaagtt agagggcgc caaagttca atagttaca gtgaaactc ggttataac gaacactca aggaacttt acaattggt acagtcact atcaaaaag aatgacacc cctaccaac tggtagttt atgaactag ccaaacgta cacattaat cgcattata tcgaaaaga tttctagct actgtttta gcggtgtca ttctttgtg gtcgtgact gtacgttat aaccgatcg ttcgttcta accgaacat catctttat tgagtaatt tctaagtta aatgaattc gaaagcttg gaatgatgc acaaaataa agtattaat ctaatttat taagaaaga tacatttac atacatggt gtcccctat tgaccttta aatgttgaa tttttttga gctgcaata caaacacgc atagttt