Status
Please wait ...
Gene information
General information
TC003811 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 20996037..21025116
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003811
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03627

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003811 RA
      MSDIKSMFA KMATTKDSG GGGDNGESP PKPPAPKPK 
      PKPKQTIEE TYQKKSQLE HILLRPDTY IGSVEKVDE 
      NMWVYDRDT EMIIQKNIS YVPGLYKIF DEILVNAAD 
      NKQRDNTMD CIKIEINQD DNVISVWNN GQGIPVAMH 
      KSENMYVPT MIFGHLLTS SNYNDKEEK VTGGRNGYG 
      AKLCNIFST KFTVETATK EYKRHFKQV WGNNMTKAS 
      EPKIKDHFG EDFTKITFS PDLVKFKME KLEDDIVSL 
      MCRRAFDVA ASTRGVKVF LNGKRVPVK NFKDYVDFF 
      TKGKDEDGN EKCKVVYEV CNERWEVAM TLSDRGFQQ 
      MSFVNSIAT TKGGRHVDY VTEMIVKQI IEVLKKRNK 
      GGVAIKPFQ VKNHMWIFI NCLIVNPTF DSQTKENMT 
      LQAKSFGSK CTLSEKFIT QVVKSGIVE SVLSWAKFK 
      AQTELEKKT AGKKQSKLK GIPKLEDAN EAGTKNGLR 
      CTLILTEGD SAKSLAVSG LGVIGRDYY GVFPLRGKL 
      LNVREATHK QILENQEIN NLIKILGLQ YKKKYNTDE 
      DMRTLRYGK VMIMTDQDQ DGSHIKGLL INFIHYNWP 
      ELLRRDFLE EFITPIVKA KKRNETLSF YSLPEFEEW 
      KSNTPNHRT YQIKYYKGL GTSDSKEAK EYFSDMARH 
      RIRFRYKGP EDDDHIVLA FSKKHIEHR KEWLTNFMV 
      ESRRRKELG LAEKYLYAK DTKSVSYTD FVNLELVLF 
      SNADNIRSI PCLVDGLKP GQRKVMFTC FKRNDKREV 
      KVAQLAGSV AEMSAYHHG EVSLCMTIV NLAQNFVGS 
      NNINLLEPR GQFGTRMAG GKDSASPRY IFTKMSPLT 
      RMIFNPSDD YLLKHEFED NQKIEPVWY IPIIPMVLV 
      NGADGIGTG WMTKIPNYN PRELVKNIR AMLDQEEPT 
      NMVPWYKNF IGSIVDCGD NRYVASGEI AIIGDNRVE 
      ITELPIGTW TQNYKENVL EPLLHGSEK VKAVIQDYK 
      EYNTDTTVK FIITFLPGQ LETLENEAG LHKTFKLQS 
      LISTSSMCV FDELGCIRR YDSVTDILK DYYKLRLTM 
      YQKRKDYLE GMLKAEASK LSNQARFIM EKCDRTLVV 
      ENKKRKVII DELIKRGYE PDPVKDWKQ RVATEDGDE 
      EAPAEEEEA PATEQTTKK PVDPEKAFQ NSTDVQKFD 
      YLLGMSMWM LTEEKKNEL LRQRDIKLQ ELETLKKKT 
      HCDLWREDL DAFMEKLDE VEEKERNDE AGIKPKKAQ 
      AGRKKINLA ETMPSPIAK RVEPKISDD MKKRIQTAI 
      KDKKKKMVK KESTIEELE EKDEFDLLV QNKKTLKDK 
      IGTPEEAEK KVKAKKAKD ELKQTKLNF KKKSPEPKL 
      NGTPKKRGK KAKTTSSDE GDDDEFNSD FESPVVRER 
      APTKRQAAA KQIKYNLNS SEDEDSGEA ELFDNDAVI 
      EPKTNQGTM EISSDSENE SPPPRHETS EDLFDSLVG 
      RTKSDDDVP AKTETQKRR ILSDSDSDV PEEETKKSK 
      PKKKKVEAN NEDKKPKQV RKKKKKSDD EEGSGKKAK 
      GKGKKKKDL DSDVEIDSS SPPPVKSSR APRNIKQTK 
      YVFDSDESD
    CDS sequence
    • >TC003811 RA
      atgtcggat attaagagt atgtttgcc aaaatggca 
      actacaaag gacagtggc ggcggcggt gacaacgga 
      gaatcacct ccaaaaccc ccagctcca aaacccaag 
      ccgaagcca aaacaaact attgaggaa acataccag 
      aagaagtca cagttggaa catatcttg ctccgtcct 
      gacacctac atcggttct gtcgaaaaa gtggatgag 
      aatatgtgg gtgtacgat agagacaca gagatgatt 
      attcagaaa aatatctca tatgtgccg ggcttgtat 
      aagattttt gatgagatt ttggtaaac gctgcagac 
      aacaaacaa agagataat acaatggat tgtataaaa 
      atcgaaatt aaccaagat gataatgtc atatcggtg 
      tggaataat ggacaaggt attcccgtc gcaatgcac 
      aaaagcgaa aacatgtac gtcccgacg atgattttt 
      ggtcatttg ttgacctct tcgaattat aacgacaaa 
      gaggagaaa gttacaggc ggtagaaat ggttacggt 
      gcgaagttg tgtaacatt ttcagtaca aaatttact 
      gtcgaaact gccactaaa gagtataag cgtcatttt 
      aaacaagtg tggggtaat aatatgacg aaagcatcg 
      gaaccgaaa atcaaagat catttcggt gaagatttc 
      acgaaaatt accttcagt ccagatttg gtcaaattc 
      aaaatggag aaattagaa gacgacatt gtcagttta 
      atgtgtcga cgagctttc gatgtggct gcgtcaact 
      agaggcgtg aaagttttc ttgaatggc aaaagagtt 
      cccgttaaa aatttcaaa gattacgtt gactttttc 
      acaaaagga aaagacgag gacggaaac gagaaatgt 
      aaagttgtg tatgaagtt tgcaacgaa cgttgggag 
      gtcgcaatg accttatcc gaccgaggc ttccaacaa 
      atgtccttt gtcaatagt atagccacc actaaaggt 
      ggccgtcac gtggactat gttaccgaa atgatcgtt 
      aaacaaatc attgaagtg ctcaaaaaa agaaacaaa 
      ggaggagtg gcaatcaaa ccgttccaa gtcaaaaac 
      cacatgtgg atcttcatc aattgtctc atcgtgaat 
      ccaacattc gattcccaa actaaggaa aacatgaca 
      cttcaggcg aaaagtttc ggatcgaaa tgcaccctc 
      tccgagaaa ttcatcacc caagttgtg aaaagtggc 
      attgtcgag tcggttctc agctgggct aaattcaag 
      gctcaaacc gaactcgag aagaaaact gctggaaaa 
      aaacaatcc aaactgaaa ggcattccg aaactggaa 
      gacgccaac gaggcggga acgaaaaac ggtctgcgt 
      tgtacgttg attttgact gagggagac tcagcgaag 
      tctctggcg gtgtcaggt cttggtgtt attgggaga 
      gattactac ggagtgttt cccttgagg ggtaaactt 
      ctaaatgtt cgcgaagcg acgcacaaa caaattttg 
      gaaaatcag gaaatcaac aatttgatt aaaatcctt 
      ggtttgcaa tataagaaa aagtacaat accgatgaa 
      gatatgcga acgttgcgt tatggcaaa gtgatgatt 
      atgactgat caggatcag gacgggtcc catattaag 
      gggcttttg attaatttt attcattat aattggccg 
      gagttgcta aggagggat tttctcgag gagttcatt 
      actccgatt gtaaaggca aagaagcga aatgagaca 
      ctgtcgttc tattcactg ccagagttt gaagaatgg 
      aagtcgaat acgcccaat catcgcaca tatcaaatc 
      aaatactac aaaggtttg ggtacttcg gactcaaaa 
      gaggcgaaa gaatacttc agcgacatg gcgcgccac 
      cgtatccgc ttccgctac aagggcccc gaagacgac 
      gatcacatc gtactcgcc ttcagtaag aagcacatc 
      gagcatcgt aaagaatgg ctcacgaat ttcatggtc 
      gagagcaga agacgaaaa gagctcggt ctcgccgag 
      aaatatctc tacgcaaaa gacacaaaa tccgtctca 
      tacacggat ttcgttaat ctcgaattg gttctcttc 
      tcgaacgcc gacaatatt cgctcgatt ccatgtctg 
      gtggacggt cttaaaccc ggccagcgt aaagtaatg 
      ttcacctgc ttcaaacgt aacgataaa cgtgaggta 
      aaagtggcc cagttggcg ggttctgtg gctgaaatg 
      tcggcgtac catcacggc gaagtgtcc ctttgtatg 
      acaatcgtt aacttggct caaaatttt gtcggtagc 
      aataatatt aatttgttg gagccgaga ggtcagttc 
      ggtacgcgg atggcggga gggaaagac agcgccagt 
      ccgagatac attttcaca aagatgtcg cctttaact 
      cggatgatt tttaatcct tccgatgat tatttgttg 
      aaacatgag tttgaagat aatcagaag attgaaccg 
      gtgtggtat attcctatc ataccgatg gttttggtt 
      aatggtgct gacggtata ggcaccggt tggatgacg 
      aaaattccc aactacaat ccacgagaa cttgtaaag 
      aatataaga gcgatgttg gatcaagag gaaccgacg 
      aatatggtt ccttggtat aaaaatttt atcgggagt 
      atagtcgat tgtggcgat aacagatac gtggccagt 
      ggagaaatt gctataata ggggacaac cgagtcgaa 
      attactgaa ttgcccatt ggaacgtgg actcagaat 
      tacaaggaa aacgtcttg gagcctcta ctgcacggg 
      tccgaaaaa gtaaaggcc gtaattcaa gattacaaa 
      gaatacaac acggataca acggttaaa ttcataata 
      actttcttg ccgggccaa ttggaaacc ctagaaaat 
      gaggcaggt ctccataaa actttcaaa ttacaatcc 
      ttgatctca acaagttcc atgtgcgtt tttgacgaa 
      ctcggatgc atacgaagg tatgattcg gtaaccgac 
      atattgaag gactattac aagttacgt ttgaccatg 
      taccaaaaa cgtaaagat taccttgaa ggtatgcta 
      aaagctgaa gcgagtaaa ctatcaaat caagctcgg 
      tttataatg gagaaatgc gaccgtact ttggtcgtt 
      gaaaacaag aaacgtaaa gtgattatt gacgaactt 
      atcaaaaga gggtacgaa ccagatcct gtcaaggac 
      tggaaacaa agggttgct acggaagat ggagatgaa 
      gaagcgcca gctgaagag gaagaagca ccagcgact 
      gaacagaca acgaagaaa ccagttgat ccagaaaag 
      gctttccaa aattcgacc gatgttcaa aagtttgat 
      tatttattg ggaatgtca atgtggatg ttgacggaa 
      gaaaagaag aatgaattg ctacgacaa agagacatt 
      aaattacaa gaattggag actttgaaa aagaaaacg 
      cattgtgat ttgtggcgg gaggatctc gatgcgttt 
      atggagaag ttggatgaa gtggaagag aaggaacgg 
      aatgacgaa gctggaatt aaaccgaag aaagcacaa 
      gcgggaagg aagaaaatt aatttggca gaaacaatg 
      ccatcacct atagccaaa cgagtcgaa ccgaaaatt 
      tccgatgat atgaagaag cgcatacag accgcaata 
      aaagataag aaaaagaag atggtcaaa aaagaaagc 
      acgattgaa gaattggaa gaaaaagac gaatttgat 
      cttctggta caaaacaag aagacttta aaagacaaa 
      ataggcact ccggaagag gcagagaag aaagtgaaa 
      gcgaaaaag gccaaagat gagcttaaa caaactaag 
      ctcaatttt aaaaagaag agtccggag ccaaaactg 
      aatggtacc ccgaaaaaa cgcgggaaa aaggcgaag 
      accaccagt tctgacgaa ggtgacgat gacgaattc 
      aattccgat tttgaaagt cctgttgtg agagagcga 
      gcgccgact aagcgccaa gccgcagca aaacaaatt 
      aaatacaat ttgaattct tcggaagat gaggatagt 
      ggtgaagcg gaactgttc gataacgac gccgtaatc 
      gagcccaaa actaaccaa gggacgatg gaaattagc 
      tcagacagc gaaaatgaa tctcctcca ccgaggcat 
      gaaacctcg gaagatctg ttcgattct cttgtaggt 
      cggacgaag agcgatgat gacgtacct gcaaaaact 
      gaaactcag aagagaagg attttgtcg gattcagat 
      tcggatgtt cccgaagaa gaaacgaaa aaatctaaa 
      ccgaagaag aaaaaggtt gaggcaaat aatgaagac 
      aaaaaacct aagcaagtc agaaagaag aagaagaag 
      tcagatgat gaggaggga agtgggaaa aaagcaaaa 
      gggaaaggt aaaaagaag aaggattta gattcagat 
      gttgaaatt gattcttct tcaccacca ccggttaag 
      tcttcacga gctccacga aatattaaa caaactaaa 
      tatgtattt gattcggac gagtctgac tga
    mRNA sequence
    • >TC003811 RA
      attcaaaaa ttttacgaa gatagccga agtcggatt 
      tttttcttg cgaaaagcg gaagaaatc gagcggtct 
      gttgaaggt gttatcggc cattcgtgt ttatctgtt 
      ttaaatagt aaaaagaga gttttgcta caatttggt 
      gtttaaaat gttgtgaaa atattcttg tggcgtgaa 
      aatgtcgga tattaagag tatgtttgc caaaatggc 
      aactacaaa ggacagtgg cggcggcgg tgacaacgg 
      agaatcacc tccaaaacc cccagctcc aaaacccaa 
      gccgaagcc aaaacaaac tattgagga aacatacca 
      gaagaagtc acagttgga acatatctt gctccgtcc 
      tgacaccta catcggttc tgtcgaaaa agtggatga 
      gaatatgtg ggtgtacga tagagacac agagatgat 
      tattcagaa aaatatctc atatgtgcc gggcttgta 
      taagatttt tgatgagat tttggtaaa cgctgcaga 
      caacaaaca aagagataa tacaatgga ttgtataaa 
      aatcgaaat taaccaaga tgataatgt catatcggt 
      gtggaataa tggacaagg tattcccgt cgcaatgca 
      caaaagcga aaacatgta cgtcccgac gatgatttt 
      tggtcattt gttgacctc ttcgaatta taacgacaa 
      agaggagaa agttacagg cggtagaaa tggttacgg 
      tgcgaagtt gtgtaacat tttcagtac aaaatttac 
      tgtcgaaac tgccactaa agagtataa gcgtcattt 
      taaacaagt gtggggtaa taatatgac gaaagcatc 
      ggaaccgaa aatcaaaga tcatttcgg tgaagattt 
      cacgaaaat taccttcag tccagattt ggtcaaatt 
      caaaatgga gaaattaga agacgacat tgtcagttt 
      aatgtgtcg acgagcttt cgatgtggc tgcgtcaac 
      tagaggcgt gaaagtttt cttgaatgg caaaagagt 
      tcccgttaa aaatttcaa agattacgt tgacttttt 
      cacaaaagg aaaagacga ggacggaaa cgagaaatg 
      taaagttgt gtatgaagt ttgcaacga acgttggga 
      ggtcgcaat gaccttatc cgaccgagg cttccaaca 
      aatgtcctt tgtcaatag tatagccac cactaaagg 
      tggccgtca cgtggacta tgttaccga aatgatcgt 
      taaacaaat cattgaagt gctcaaaaa aagaaacaa 
      aggaggagt ggcaatcaa accgttcca agtcaaaaa 
      ccacatgtg gatcttcat caattgtct catcgtgaa 
      tccaacatt cgattccca aactaagga aaacatgac 
      acttcaggc gaaaagttt cggatcgaa atgcaccct 
      ctccgagaa attcatcac ccaagttgt gaaaagtgg 
      cattgtcga gtcggttct cagctgggc taaattcaa 
      ggctcaaac cgaactcga gaagaaaac tgctggaaa 
      aaaacaatc caaactgaa aggcattcc gaaactgga 
      agacgccaa cgaggcggg aacgaaaaa cggtctgcg 
      ttgtacgtt gattttgac tgagggaga ctcagcgaa 
      gtctctggc ggtgtcagg tcttggtgt tattgggag 
      agattacta cggagtgtt tcccttgag gggtaaact 
      tctaaatgt tcgcgaagc gacgcacaa acaaatttt 
      ggaaaatca ggaaatcaa caatttgat taaaatcct 
      tggtttgca atataagaa aaagtacaa taccgatga 
      agatatgcg aacgttgcg ttatggcaa agtgatgat 
      tatgactga tcaggatca ggacgggtc ccatattaa 
      ggggctttt gattaattt tattcatta taattggcc 
      ggagttgct aaggaggga ttttctcga ggagttcat 
      tactccgat tgtaaaggc aaagaagcg aaatgagac 
      actgtcgtt ctattcact gccagagtt tgaagaatg 
      gaagtcgaa tacgcccaa tcatcgcac atatcaaat 
      caaatacta caaaggttt gggtacttc ggactcaaa 
      agaggcgaa agaatactt cagcgacat ggcgcgcca 
      ccgtatccg cttccgcta caagggccc cgaagacga 
      cgatcacat cgtactcgc cttcagtaa gaagcacat 
      cgagcatcg taaagaatg gctcacgaa tttcatggt 
      cgagagcag aagacgaaa agagctcgg tctcgccga 
      gaaatatct ctacgcaaa agacacaaa atccgtctc 
      atacacgga tttcgttaa tctcgaatt ggttctctt 
      ctcgaacgc cgacaatat tcgctcgat tccatgtct 
      ggtggacgg tcttaaacc cggccagcg taaagtaat 
      gttcacctg cttcaaacg taacgataa acgtgaggt 
      aaaagtggc ccagttggc gggttctgt ggctgaaat 
      gtcggcgta ccatcacgg cgaagtgtc cctttgtat 
      gacaatcgt taacttggc tcaaaattt tgtcggtag 
      caataatat taatttgtt ggagccgag aggtcagtt 
      cggtacgcg gatggcggg agggaaaga cagcgccag 
      tccgagata cattttcac aaagatgtc gcctttaac 
      tcggatgat ttttaatcc ttccgatga ttatttgtt 
      gaaacatga gtttgaaga taatcagaa gattgaacc 
      ggtgtggta tattcctat cataccgat ggttttggt 
      taatggtgc tgacggtat aggcaccgg ttggatgac 
      gaaaattcc caactacaa tccacgaga acttgtaaa 
      gaatataag agcgatgtt ggatcaaga ggaaccgac 
      gaatatggt tccttggta taaaaattt tatcgggag 
      tatagtcga ttgtggcga taacagata cgtggccag 
      tggagaaat tgctataat aggggacaa ccgagtcga 
      aattactga attgcccat tggaacgtg gactcagaa 
      ttacaagga aaacgtctt ggagcctct actgcacgg 
      gtccgaaaa agtaaaggc cgtaattca agattacaa 
      agaatacaa cacggatac aacggttaa attcataat 
      aactttctt gccgggcca attggaaac cctagaaaa 
      tgaggcagg tctccataa aactttcaa attacaatc 
      cttgatctc aacaagttc catgtgcgt ttttgacga 
      actcggatg catacgaag gtatgattc ggtaaccga 
      catattgaa ggactatta caagttacg tttgaccat 
      gtaccaaaa acgtaaaga ttaccttga aggtatgct 
      aaaagctga agcgagtaa actatcaaa tcaagctcg 
      gtttataat ggagaaatg cgaccgtac tttggtcgt 
      tgaaaacaa gaaacgtaa agtgattat tgacgaact 
      tatcaaaag agggtacga accagatcc tgtcaagga 
      ctggaaaca aagggttgc tacggaaga tggagatga 
      agaagcgcc agctgaaga ggaagaagc accagcgac 
      tgaacagac aacgaagaa accagttga tccagaaaa 
      ggctttcca aaattcgac cgatgttca aaagtttga 
      ttatttatt gggaatgtc aatgtggat gttgacgga 
      agaaaagaa gaatgaatt gctacgaca aagagacat 
      taaattaca agaattgga gactttgaa aaagaaaac 
      gcattgtga tttgtggcg ggaggatct cgatgcgtt 
      tatggagaa gttggatga agtggaaga gaaggaacg 
      gaatgacga agctggaat taaaccgaa gaaagcaca 
      agcgggaag gaagaaaat taatttggc agaaacaat 
      gccatcacc tatagccaa acgagtcga accgaaaat 
      ttccgatga tatgaagaa gcgcataca gaccgcaat 
      aaaagataa gaaaaagaa gatggtcaa aaaagaaag 
      cacgattga agaattgga agaaaaaga cgaatttga 
      tcttctggt acaaaacaa gaagacttt aaaagacaa 
      aataggcac tccggaaga ggcagagaa gaaagtgaa 
      agcgaaaaa ggccaaaga tgagcttaa acaaactaa 
      gctcaattt taaaaagaa gagtccgga gccaaaact 
      gaatggtac cccgaaaaa acgcgggaa aaaggcgaa 
      gaccaccag ttctgacga aggtgacga tgacgaatt 
      caattccga ttttgaaag tcctgttgt gagagagcg 
      agcgccgac taagcgcca agccgcagc aaaacaaat 
      taaatacaa tttgaattc ttcggaaga tgaggatag 
      tggtgaagc ggaactgtt cgataacga cgccgtaat 
      cgagcccaa aactaacca agggacgat ggaaattag 
      ctcagacag cgaaaatga atctcctcc accgaggca 
      tgaaacctc ggaagatct gttcgattc tcttgtagg 
      tcggacgaa gagcgatga tgacgtacc tgcaaaaac 
      tgaaactca gaagagaag gattttgtc ggattcaga 
      ttcggatgt tcccgaaga agaaacgaa aaaatctaa 
      accgaagaa gaaaaaggt tgaggcaaa taatgaaga 
      caaaaaacc taagcaagt cagaaagaa gaagaagaa 
      gtcagatga tgaggaggg aagtgggaa aaaagcaaa 
      agggaaagg taaaaagaa gaaggattt agattcaga 
      tgttgaaat tgattcttc ttcaccacc accggttaa 
      gtcttcacg agctccacg aaatattaa acaaactaa 
      atatgtatt tgattcgga cgagtctga ctgaattca 
      actgtttta gaaaatttt aagaagtaa ctatttaat 
      gtgttttta ataggtaaa tatgtgtta cgttacttg 
      taactttaa taaaaatat cttattttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY