Status
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Gene information
General information
TC003807 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 20803322..20806009
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003807
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03626

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003807 RA
      MVLSMEKWV GKVAIVSGA SSGIGAAIV DQLLEHGLI 
      VVGAARRSE LVEERAKKL SNKKGKLHA IKADFTKEE 
      DILRVFEWT SKNLGPVHI LINNAGTTR NSTLTEGKT 
      DDWKAVLDL NVLGLCTAT REAVKIMKT NNINGHIIH 
      LNSILGHTI CMGHMINVY GAAKFAVTA LTETLRQEL 
      NSLNLKIKI TSVSPGLVE SEMTTLKKD QPPERKALF 
      ETMPILKAE DIADGVVYA LSTPEHVQV HELTIKPVG 
      ESF
    CDS sequence
    • >TC003807 RA
      atggttctc tcaatggaa aaatgggtt ggcaaagtg 
      gccatagta agtggtgct agttcggga atcggtgct 
      gcaatagtc gaccaatta ctcgagcac ggtttaatt 
      gttgttgga gctgctcgc cgctcggaa ttagtagaa 
      gaacgcgct aaaaaactt tcaaacaaa aagggaaaa 
      ctccacgca atcaaagcc gattttact aaagaggaa 
      gacattttg cgagttttc gaatggacg agtaaaaat 
      cttggccct gtccacatt ctcataaat aatgctgga 
      actacacga aactcaact ttgactgaa ggaaaaacc 
      gatgactgg aaagcagtt ttggatttg aacgttcta 
      ggattatgc actgcgacg agggaagcg gtcaaaatc 
      atgaaaacc aataacata aacggacac attatccat 
      ctgaacagt attttgggg cacactata tgtatggga 
      catatgatc aacgtttac ggagccgca aaatttgcc 
      gtcactgca ttgactgag acattgagg caagaattg 
      aatagtctc aacctcaaa attaaaata actagtgta 
      agtccgggt ttggtcgag tccgagatg acaacattg 
      aagaaagat caaccaccg gagagaaaa gcattgttt 
      gagacaatg ccgattttg aaagctgag gatattgct 
      gatggggtt gtttatgct ttatctacc ccagaacat 
      gtccaagtt cacgaactc acaataaaa ccagtcgga 
      gagtcattt taa
    mRNA sequence
    • >TC003807 RA
      gaattaaag taaatgttt atcccgttt gagctcagt 
      tgagatata gttttacgt tcagcacaa tggttctct 
      caatggaaa aatgggttg gcaaagtgg ccatagtaa 
      gtggtgcta gttcgggaa tcggtgctg caatagtcg 
      accaattac tcgagcacg gtttaattg ttgttggag 
      ctgctcgcc gctcggaat tagtagaag aacgcgcta 
      aaaaacttt caaacaaaa agggaaaac tccacgcaa 
      tcaaagccg attttacta aagaggaag acattttgc 
      gagttttcg aatggacga gtaaaaatc ttggccctg 
      tccacattc tcataaata atgctggaa ctacacgaa 
      actcaactt tgactgaag gaaaaaccg atgactgga 
      aagcagttt tggatttga acgttctag gattatgca 
      ctgcgacga gggaagcgg tcaaaatca tgaaaacca 
      ataacataa acggacaca ttatccatc tgaacagta 
      ttttggggc acactatat gtatgggac atatgatca 
      acgtttacg gagccgcaa aatttgccg tcactgcat 
      tgactgaga cattgaggc aagaattga atagtctca 
      acctcaaaa ttaaaataa ctagtgtaa gtccgggtt 
      tggtcgagt ccgagatga caacattga agaaagatc 
      aaccaccgg agagaaaag cattgtttg agacaatgc 
      cgattttga aagctgagg atattgctg atggggttg 
      tttatgctt tatctaccc cagaacatg tccaagttc 
      acgaactca caataaaac cagtcggag agtcatttt 
      aagaaattg caaagaaac agtttcaaa taaaaatgg 
      aataatatt cttttacaa atatgttat actaaacac 
      ccttacaag tgaaactct agaattttt tctaattaa 
      ttatatcaa aaatttgtt tagtttaga ctaaaataa 
      agatggaaa tccaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY