Status
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Gene information
General information
TC032553
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003802,TC003801
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 16335589..16523932
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032553
    iBeetle screen iB_03624

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    remarks: later than schedule, hours 48 remarks: later than schedule, hours 48 remarks: later than schedule, hours 48
    Pupal morphology
    remarks: later than schedule, hours 48
    Adult morphology
    remarks: later than schedule, hours 48 remarks: later than schedule, hours 48
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 remarks: later than schedule, hours 48 (egglay)
    remarks: later than schedule, hours 48
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    remarks: later than schedule, hours 48
    remarks - cuticles: later than schedule, hours 48
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032553 RA
      MNVVVFLLN VLIVVVVSG GPAWREVQR GGGLRVGSG 
      AGKNSSSSR GNGIRVGGS TRGVRITTT MMPEGDQVS 
      QPTATATSN HQHTPLNVG MVLPSKSFG VREYTKAVT 
      SAKYNLQRK LRLFKHHDI KVHIVMKEL TPSPTAILK 
      SLCKEFLSV NVSAILYLM NYEKYGRST ASAQYFLQL 
      AGYLGIPVI AWNADNSGL ERRASQSSL QLQLAPSLE 
      HQTAAMLSI LERYKWHQF SVVTSPIAG HDDFIQAVR 
      ERVSAMQDR FKFTILNAV LVSHHRDLA ALVDSEARV 
      MLLYCTSQE AIDILTAAK DFHLTGENY VWVVTQSVI 
      ANPLEAPGQ FPVGMLGVH FDTSSSSLV NEITTAIKV 
      YAYGVEDFT NDLANAGRS LNTQLSCEG EGAARWNTG 
      DRFFRVLRN VSVEGEAGK PNLEFTQDG VLKAAELKI 
      MNLRPGVSK QLVWEEIGV WKSWQKEGL DIKDIVWPG 
      NSHTPPQGV PEKFHLKIT FLEEPPYIS LAPPDPVTG 
      KCSMDRGVL CRIASDADI TEVDTTLAH RNGSFYQCC 
      SGFCIDLLQ KFSEELGFT YELVRVEDG RWGTNENGK 
      WNGLIADLV NRKTDMVLT SLMINAERE AVVDFSVPF 
      METGIAIVV AKRTGIISP TAFLEPFDT ASWMLVGVV 
      AIQAATFTI FLFEWLSPS GFNMRLSLN QSTPSASHR 
      FSLFRTYWL VWAVLFQAA VHVDSPRGF TARFMTNVW 
      AMFAVVFLA IYTANLAAF MITREEFFE FSGLDDHRL 
      SRPYSQKPL IKFGTIPWS HTDSTIAKY FKEMHAYMR 
      QFNKSTVHE GVDAVLSAE MDAFIYDGT VLDYLTSQD 
      EDCRLLTVG SWYAMTGYG LAFPRNSKY LKMFNKRLL 
      DFRENGDLE RLRRYWMTG VCKPGKQEH KSSDPLALE 
      QFLSAFLLL MAGILLAAL LLFLEHLYF KYVRKHLAK 
      TDRGGCCAL ISLSMGKSL TFRGAVYEA QDILRHHRC 
      RDPICDTHL WKVKRELDI SQMRCKQLE KELEAHGIK 
      PPPPCKRVI VSGEQARSR LRSLNPDKS SNSDLFGQK 
      TEIAEMETV L
    • >TC032553 RB
      MNVVVFLLN VLIVVVVSG GPAWREVQR GGGLRVGSG 
      AGKNSSSSR GNGIRVGGS TRGVRITTT MMPEGDQVS 
      QPTATATSN HQHTPLNVG MVLPSKSFG VREYTKAVT 
      SAKYNLQRK LRLFKHHDI KVHIVMKEL TPSPTGNSM 
      AGDHKDSLN VGLILPYTN FGVREYTRA INNAVSGLH 
      RSRGQRLNW LKKYNFTPK NVHYVLITL TPSPTAILK 
      SLCKEFLSV NVSAILYLM NYEKYGRST ASAQYFLQL 
      AGYLGIPVI AWNADNSGL ERRASQSSL QLQLAPSLE 
      HQTAAMLSI LERYKWHQF SVVTSPIAG HDDFIQAVR 
      ERVSAMQDR FKFTILNAV LVSHHRDLA ALVDSEARV 
      MLLYCTSQE AIDILTAAK DFHLTGENY VWVVTQSVI 
      ANPLEAPGQ FPVGMLGVH FDTSSSSLV NEITTAIKV 
      YAYGVEDFT NDLANAGRS LNTQLSCEG EGAARWNTG 
      DRFFRVLRN VSVEGEAGK PNLEFTQDG VLKAAELKI 
      MNLRPGVSK QLVWEEIGV WKSWQKEGL DIKDIVWPG 
      NSHTPPQGV PEKFHLKIT FLEEPPYIS LAPPDPVTG 
      KCSMDRGVL CRIASDADI TEVDTTLAH RNGSFYQCC 
      SGFCIDLLQ KFSEELGFT YELVRVEDG RWGTNENGK 
      WNGLIADLV NRKTDMVLT SLMINAERE AVVDFSVPF 
      METGIAIVV AKRTGIISP TAFLEPFDT ASWMLVGVV 
      AIQAATFTI FLFEWLSPS GFNMRLSLN QSTPSASHR 
      FSLFRTYWL VWAVLFQAA VHVDSPRGF TARFMTNVW 
      AMFAVVFLA IYTANLAAF MITREEFFE FSGLDDHRL 
      SRPYSQKPL IKFGTIPWS HTDSTIAKY FKEMHAYMR 
      QFNKSTVHE GVDAVLSAE MDAFIYDGT VLDYLTSQD 
      EDCRLLTVG SWYAMTGYG LAFPRNSKY LKMFNKRLL 
      DFRENGDLE RLRRYWMTG VCKPGKQEH KSSDPLALE 
      QFLSAFLLL MAGILLAAL LLFLEHLYF KYVRKHLAK 
      TDRGGCCAL ISLSMGKSL TFRGAVYEA QDILRHHRC 
      RDPICDTHL WKVKRELDI SQMRCKQLE KELEAHGIK 
      PPPPCKRVI VSGEQARSR LRSLNPDKS SNSDLFGQK 
      TEIAEMETV L
    CDS sequence
    • >TC032553 RA
      atgaatgtg gtggtcttc ctgctgaat gtgctgatt 
      gtggtggtg gtcagtggc gggcccgct tggcgagaa 
      gtccaaagg ggcggcggg ctgagggtc ggctccggc 
      gccggcaaa aactcctct tcgtcgaga ggcaacgga 
      attagggtc ggcggcagc accagaggc gttcggatc 
      accaccacg atgatgccg gaaggcgac caagtcagc 
      caaccgacc gctacggcc acttccaac caccagcat 
      acgcctctc aatgtgggg atggtgctg ccgtccaag 
      tccttcggg gtgagggag tacaccaag gctgtcact 
      tcggccaag tacaacttg cagaggaaa ttacggctt 
      ttcaagcac cacgatatc aaggttcac atcgtgatg 
      aaagaactc acccctagt ccaacagca attctcaag 
      tccctctgc aaggaattc ctttcggtt aatgtgtca 
      gccatttta tatttaatg aattacgag aaatacggt 
      cgaagtacg gcatctgca cagtatttc cttcaatta 
      gccggctac ttagggata cctgtcatc gcctggaat 
      gcggataat tcaggattg gaaaggaga gcatcacaa 
      tcttccctc caacttcaa ttagcgcca tcgctggaa 
      catcaaaca gctgcgatg ttgagcata ttagagaga 
      tataagtgg catcaattt tcagtggta accagtccg 
      atcgcagga cacgatgat ttcatacag gctgtgcgg 
      gagcgagtc tcggcaatg caggaccgc ttcaagttt 
      acgatcctc aacgcagtc ctcgtatca caccatcgc 
      gacttggct gcacttgtg gattctgaa gcccgagta 
      atgctgctt tattgtacg agtcaagaa gcaatagac 
      atacttact gccgctaag gattttcac cttactgga 
      gaaaattat gtttgggtt gtaacacaa agcgttata 
      gcgaaccca cttgaagca ccaggccaa tttccagtt 
      ggcatgtta ggtgtacat tttgacaca tcgagttcc 
      agtctggta aacgagata acgaccgca atcaaagtg 
      tatgcttat ggtgtggaa gacttcaca aacgacctg 
      gcaaatgca gggcgctcc cttaatacc caactttcg 
      tgcgaaggc gaaggtgca gctcgatgg aataccggt 
      gatcggttt tttcgggta cttcgcaat gtgagtgtt 
      gaaggcgaa gccgggaaa cccaatctg gaattcacg 
      caagacgga gtgttgaaa gcggctgag ttgaaaata 
      atgaatctt aggccagga gttagcaaa cagctcgtt 
      tgggaagag attggagtt tggaagtcc tggcagaag 
      gagggttta gacattaaa gacattgtc tggccggga 
      aatagccac acgccaccg caaggagtt cctgagaag 
      tttcatcta aaaatcact tttttggaa gaaccacca 
      tacataagc ctagcacct cctgacccc gttacagga 
      aagtgttca atggatagg ggggtgttg tgcaggatt 
      gccagcgac gcagacatc accgaggtc gacacgacg 
      ctggcccac cgcaacggc agtttctat cagtgctgc 
      tctggattt tgcatcgac cttttgcaa aaattctcc 
      gaagagttg ggtttcacc tacgaactc gtccgtgtt 
      gaagacggg agatgggga acgaacgaa aatggaaag 
      tggaacggc ctcattgct gatctagtt aatcgaaaa 
      acagatatg gttctgacg tcgctgatg attaatgcg 
      gaaagagag gcagtggta gacttcagt gttcctttt 
      atggagacg ggaattgca atcgttgtg gccaaacga 
      actggaatt atttctccg acggcattt ttggaaccg 
      tttgataca gcctcttgg atgcttgtg ggggttgtc 
      gcaattcaa gctgccacc ttcaccatt ttccttttt 
      gaatggctg tctccgagt ggtttcaat atgagattg 
      tcgcttaat cagtcaacg ccatcagcg tcacatcgt 
      ttttcgtta ttccgcact tactggctg gtttgggct 
      gtccttttc caagcggcg gttcatgtt gattctccc 
      agaggattc acagcacgt tttatgaca aatgtttgg 
      gcgatgttc gccgtagtc ttccttgcc atatacact 
      gccaacctc gcagccttc atgattacc agagaagag 
      ttttttgaa ttttcaggt ctcgacgac caccgctta 
      tcgcggcca tactcgcaa aagccactc atcaaattc 
      ggaacaatt ccctggtct cacaccgat tcaacaata 
      gcgaaatat ttcaaagaa atgcacgct tacatgaga 
      caattcaac aaatccaca gtccatgaa ggagtcgac 
      gccgtttta agcgccgaa atggacgct ttcatatac 
      gacggcact gtcttagat tacctcact tcgcaagac 
      gaagactgc agacttctc accgtcggt tcctggtac 
      gcaatgacc ggctacggc ctggccttc ccccgaaat 
      tccaaatac ctgaaaatg ttcaacaaa cgactgctg 
      gattttcga gaaaacggc gaccttgaa cgtctgcgc 
      cgctactgg atgaccggg gtctgtaag cccggaaaa 
      caggagcac aagagctcg gacccctta gctttagaa 
      cagttctta tcagcgttc ctattgttg atggcgggg 
      atactactc gccgcgctg ctgttgttt ctcgaacac 
      ttgtacttc aaatacgtt cggaagcac ctcgcaaag 
      accgaccga ggaggctgt tgtgccctc atcagtctg 
      tctatgggc aaaagtctg acgtttcga ggggctgtg 
      tacgaggct caagacatc ctgagacat caccgctgc 
      agagacccc atttgcgac actcatctc tggaaagtc 
      aaacgggag ttggacatc tcccaaatg aggtgtaag 
      cagctggag aaggagttg gaagcgcac ggtataaag 
      ccgccgcct ccatgcaaa cgtgtaata gtttctgga 
      gaacaagcc agatctagg ttaagaagc ttaaatcca 
      gataaaagt agcaactct gatttattt ggtcagaag 
      acagaaata gcagaaatg gaaacagta ctgtga
    • >TC032553 RB
      atgaatgtg gtggtcttc ctgctgaat gtgctgatt 
      gtggtggtg gtcagtggc gggcccgct tggcgagaa 
      gtccaaagg ggcggcggg ctgagggtc ggctccggc 
      gccggcaaa aactcctct tcgtcgaga ggcaacgga 
      attagggtc ggcggcagc accagaggc gttcggatc 
      accaccacg atgatgccg gaaggcgac caagtcagc 
      caaccgacc gctacggcc acttccaac caccagcat 
      acgcctctc aatgtgggg atggtgctg ccgtccaag 
      tccttcggg gtgagggag tacaccaag gctgtcact 
      tcggccaag tacaacttg cagaggaaa ttacggctt 
      ttcaagcac cacgatatc aaggttcac atcgtgatg 
      aaagaactc acccctagt ccaacaggt aattcgatg 
      gctggagat cataaggat tcgcttaac gtcggactc 
      attttgccg tacacgaat ttcggggtc cgggaatac 
      accagagcg atcaacaat gcggtctct ggactacat 
      cgatctagg ggacagcgc ttaaattgg ctcaagaag 
      tataatttc accccgaaa aacgtccac tatgttctc 
      attactctc acgcctagt ccaacagca attctcaag 
      tccctctgc aaggaattc ctttcggtt aatgtgtca 
      gccatttta tatttaatg aattacgag aaatacggt 
      cgaagtacg gcatctgca cagtatttc cttcaatta 
      gccggctac ttagggata cctgtcatc gcctggaat 
      gcggataat tcaggattg gaaaggaga gcatcacaa 
      tcttccctc caacttcaa ttagcgcca tcgctggaa 
      catcaaaca gctgcgatg ttgagcata ttagagaga 
      tataagtgg catcaattt tcagtggta accagtccg 
      atcgcagga cacgatgat ttcatacag gctgtgcgg 
      gagcgagtc tcggcaatg caggaccgc ttcaagttt 
      acgatcctc aacgcagtc ctcgtatca caccatcgc 
      gacttggct gcacttgtg gattctgaa gcccgagta 
      atgctgctt tattgtacg agtcaagaa gcaatagac 
      atacttact gccgctaag gattttcac cttactgga 
      gaaaattat gtttgggtt gtaacacaa agcgttata 
      gcgaaccca cttgaagca ccaggccaa tttccagtt 
      ggcatgtta ggtgtacat tttgacaca tcgagttcc 
      agtctggta aacgagata acgaccgca atcaaagtg 
      tatgcttat ggtgtggaa gacttcaca aacgacctg 
      gcaaatgca gggcgctcc cttaatacc caactttcg 
      tgcgaaggc gaaggtgca gctcgatgg aataccggt 
      gatcggttt tttcgggta cttcgcaat gtgagtgtt 
      gaaggcgaa gccgggaaa cccaatctg gaattcacg 
      caagacgga gtgttgaaa gcggctgag ttgaaaata 
      atgaatctt aggccagga gttagcaaa cagctcgtt 
      tgggaagag attggagtt tggaagtcc tggcagaag 
      gagggttta gacattaaa gacattgtc tggccggga 
      aatagccac acgccaccg caaggagtt cctgagaag 
      tttcatcta aaaatcact tttttggaa gaaccacca 
      tacataagc ctagcacct cctgacccc gttacagga 
      aagtgttca atggatagg ggggtgttg tgcaggatt 
      gccagcgac gcagacatc accgaggtc gacacgacg 
      ctggcccac cgcaacggc agtttctat cagtgctgc 
      tctggattt tgcatcgac cttttgcaa aaattctcc 
      gaagagttg ggtttcacc tacgaactc gtccgtgtt 
      gaagacggg agatgggga acgaacgaa aatggaaag 
      tggaacggc ctcattgct gatctagtt aatcgaaaa 
      acagatatg gttctgacg tcgctgatg attaatgcg 
      gaaagagag gcagtggta gacttcagt gttcctttt 
      atggagacg ggaattgca atcgttgtg gccaaacga 
      actggaatt atttctccg acggcattt ttggaaccg 
      tttgataca gcctcttgg atgcttgtg ggggttgtc 
      gcaattcaa gctgccacc ttcaccatt ttccttttt 
      gaatggctg tctccgagt ggtttcaat atgagattg 
      tcgcttaat cagtcaacg ccatcagcg tcacatcgt 
      ttttcgtta ttccgcact tactggctg gtttgggct 
      gtccttttc caagcggcg gttcatgtt gattctccc 
      agaggattc acagcacgt tttatgaca aatgtttgg 
      gcgatgttc gccgtagtc ttccttgcc atatacact 
      gccaacctc gcagccttc atgattacc agagaagag 
      ttttttgaa ttttcaggt ctcgacgac caccgctta 
      tcgcggcca tactcgcaa aagccactc atcaaattc 
      ggaacaatt ccctggtct cacaccgat tcaacaata 
      gcgaaatat ttcaaagaa atgcacgct tacatgaga 
      caattcaac aaatccaca gtccatgaa ggagtcgac 
      gccgtttta agcgccgaa atggacgct ttcatatac 
      gacggcact gtcttagat tacctcact tcgcaagac 
      gaagactgc agacttctc accgtcggt tcctggtac 
      gcaatgacc ggctacggc ctggccttc ccccgaaat 
      tccaaatac ctgaaaatg ttcaacaaa cgactgctg 
      gattttcga gaaaacggc gaccttgaa cgtctgcgc 
      cgctactgg atgaccggg gtctgtaag cccggaaaa 
      caggagcac aagagctcg gacccctta gctttagaa 
      cagttctta tcagcgttc ctattgttg atggcgggg 
      atactactc gccgcgctg ctgttgttt ctcgaacac 
      ttgtacttc aaatacgtt cggaagcac ctcgcaaag 
      accgaccga ggaggctgt tgtgccctc atcagtctg 
      tctatgggc aaaagtctg acgtttcga ggggctgtg 
      tacgaggct caagacatc ctgagacat caccgctgc 
      agagacccc atttgcgac actcatctc tggaaagtc 
      aaacgggag ttggacatc tcccaaatg aggtgtaag 
      cagctggag aaggagttg gaagcgcac ggtataaag 
      ccgccgcct ccatgcaaa cgtgtaata gtttctgga 
      gaacaagcc agatctagg ttaagaagc ttaaatcca 
      gataaaagt agcaactct gatttattt ggtcagaag 
      acagaaata gcagaaatg gaaacagta ctgtga
    mRNA sequence
    • >TC032553 RA
      tggtgttgc tcttttcca tcagaaagc cgtcactcc 
      ctttttctt tcgtaaatg ttccgaaca tcgcccgat 
      agaaaatcg ctctctacg cgcgtttcc ccttcgaaa 
      caaaattgt tgtgcaaat gtaaataaa taaaattcc 
      cctcgcagc gttccgtca ccaaaaaca attttcatg 
      tttggcaaa atactcaat atgaatgtg gtggtcttc 
      ctgctgaat gtgctgatt gtggtggtg gtcagtggc 
      gggcccgct tggcgagaa gtccaaagg ggcggcggg 
      ctgagggtc ggctccggc gccggcaaa aactcctct 
      tcgtcgaga ggcaacgga attagggtc ggcggcagc 
      accagaggc gttcggatc accaccacg atgatgccg 
      gaaggcgac caagtcagc caaccgacc gctacggcc 
      acttccaac caccagcat acgcctctc aatgtgggg 
      atggtgctg ccgtccaag tccttcggg gtgagggag 
      tacaccaag gctgtcact tcggccaag tacaacttg 
      cagaggaaa ttacggctt ttcaagcac cacgatatc 
      aaggttcac atcgtgatg aaagaactc acccctagt 
      ccaacagca attctcaag tccctctgc aaggaattc 
      ctttcggtt aatgtgtca gccatttta tatttaatg 
      aattacgag aaatacggt cgaagtacg gcatctgca 
      cagtatttc cttcaatta gccggctac ttagggata 
      cctgtcatc gcctggaat gcggataat tcaggattg 
      gaaaggaga gcatcacaa tcttccctc caacttcaa 
      ttagcgcca tcgctggaa catcaaaca gctgcgatg 
      ttgagcata ttagagaga tataagtgg catcaattt 
      tcagtggta accagtccg atcgcagga cacgatgat 
      ttcatacag gctgtgcgg gagcgagtc tcggcaatg 
      caggaccgc ttcaagttt acgatcctc aacgcagtc 
      ctcgtatca caccatcgc gacttggct gcacttgtg 
      gattctgaa gcccgagta atgctgctt tattgtacg 
      agtcaagaa gcaatagac atacttact gccgctaag 
      gattttcac cttactgga gaaaattat gtttgggtt 
      gtaacacaa agcgttata gcgaaccca cttgaagca 
      ccaggccaa tttccagtt ggcatgtta ggtgtacat 
      tttgacaca tcgagttcc agtctggta aacgagata 
      acgaccgca atcaaagtg tatgcttat ggtgtggaa 
      gacttcaca aacgacctg gcaaatgca gggcgctcc 
      cttaatacc caactttcg tgcgaaggc gaaggtgca 
      gctcgatgg aataccggt gatcggttt tttcgggta 
      cttcgcaat gtgagtgtt gaaggcgaa gccgggaaa 
      cccaatctg gaattcacg caagacgga gtgttgaaa 
      gcggctgag ttgaaaata atgaatctt aggccagga 
      gttagcaaa cagctcgtt tgggaagag attggagtt 
      tggaagtcc tggcagaag gagggttta gacattaaa 
      gacattgtc tggccggga aatagccac acgccaccg 
      caaggagtt cctgagaag tttcatcta aaaatcact 
      tttttggaa gaaccacca tacataagc ctagcacct 
      cctgacccc gttacagga aagtgttca atggatagg 
      ggggtgttg tgcaggatt gccagcgac gcagacatc 
      accgaggtc gacacgacg ctggcccac cgcaacggc 
      agtttctat cagtgctgc tctggattt tgcatcgac 
      cttttgcaa aaattctcc gaagagttg ggtttcacc 
      tacgaactc gtccgtgtt gaagacggg agatgggga 
      acgaacgaa aatggaaag tggaacggc ctcattgct 
      gatctagtt aatcgaaaa acagatatg gttctgacg 
      tcgctgatg attaatgcg gaaagagag gcagtggta 
      gacttcagt gttcctttt atggagacg ggaattgca 
      atcgttgtg gccaaacga actggaatt atttctccg 
      acggcattt ttggaaccg tttgataca gcctcttgg 
      atgcttgtg ggggttgtc gcaattcaa gctgccacc 
      ttcaccatt ttccttttt gaatggctg tctccgagt 
      ggtttcaat atgagattg tcgcttaat cagtcaacg 
      ccatcagcg tcacatcgt ttttcgtta ttccgcact 
      tactggctg gtttgggct gtccttttc caagcggcg 
      gttcatgtt gattctccc agaggattc acagcacgt 
      tttatgaca aatgtttgg gcgatgttc gccgtagtc 
      ttccttgcc atatacact gccaacctc gcagccttc 
      atgattacc agagaagag ttttttgaa ttttcaggt 
      ctcgacgac caccgctta tcgcggcca tactcgcaa 
      aagccactc atcaaattc ggaacaatt ccctggtct 
      cacaccgat tcaacaata gcgaaatat ttcaaagaa 
      atgcacgct tacatgaga caattcaac aaatccaca 
      gtccatgaa ggagtcgac gccgtttta agcgccgaa 
      atggacgct ttcatatac gacggcact gtcttagat 
      tacctcact tcgcaagac gaagactgc agacttctc 
      accgtcggt tcctggtac gcaatgacc ggctacggc 
      ctggccttc ccccgaaat tccaaatac ctgaaaatg 
      ttcaacaaa cgactgctg gattttcga gaaaacggc 
      gaccttgaa cgtctgcgc cgctactgg atgaccggg 
      gtctgtaag cccggaaaa caggagcac aagagctcg 
      gacccctta gctttagaa cagttctta tcagcgttc 
      ctattgttg atggcgggg atactactc gccgcgctg 
      ctgttgttt ctcgaacac ttgtacttc aaatacgtt 
      cggaagcac ctcgcaaag accgaccga ggaggctgt 
      tgtgccctc atcagtctg tctatgggc aaaagtctg 
      acgtttcga ggggctgtg tacgaggct caagacatc 
      ctgagacat caccgctgc agagacccc atttgcgac 
      actcatctc tggaaagtc aaacgggag ttggacatc 
      tcccaaatg aggtgtaag cagctggag aaggagttg 
      gaagcgcac ggtataaag ccgccgcct ccatgcaaa 
      cgtgtaata gtttctgga gaacaagcc agatctagg 
      ttaagaagc ttaaatcca gataaaagt agcaactct 
      gatttattt ggtcagaag acagaaata gcagaaatg 
      gaaacagta ctgtgatca attaagaaa atcgagatg 
      aacaacacc aaataattt ccgagtaca ttggtacca 
      tcatcattg cctgaaaca aagaactct tatgaattg 
      tggtgcatt gttaaatag tatcagtga ctacctaac 
      acataccaa tacaaaact tcttggtgc cggataagc 
      tcataattc gtcaaatac aaatagcat ttagatcga 
      aatatttat tcatattgt agatagtca gtcgcaaat 
      tcgaccgtt atgacgtta aactgactg aacgttgta 
      tagattaca tttttatca aactcaaag actaatgtt 
      gcagaacaa attattttg tcgcccaac tgtaaccta 
      taccacttt ctattagtt tacattgtt gttgctata 
      atgttgttg ctgttattg ttgatgttt tcacgctat 
      aaattatgt caatatgat gtataataa aggtcatga 
      gaaatttca aaacggaaa ttttatcac acaccacca 
      ataaacccc aacacgtat cccggaaat aaaaccgta 
      gaatttagt tgtaaaaaa tttaagaga caatgttca 
      caagactgc ctttgttgc ataagattg caaactaaa 
      tttttaaat aaattcctc ctcgacttc tagtagaat 
      taaacaaca ttgaaaatt tctaatgac cgttcagaa 
      gtacacgac aggagtctg ttttttatg tttgtttag 
      aacaaatac tttcgactc atttgtagg aagcctact 
      ccttggcct aattctaga cagttttta acagatttg 
      taaatcgaa aaatgattt gacgcttaa atgatggct 
      tagttgatg acactgtta gccactaaa tggtaaatg 
      tcttgaatg catgtccat acaaaacac tcgaaacat 
      ttaataaaa tcaatgtta gggactaaa aaattcgtc 
      tcttaaatg tgtgtaaat attaataat tttacgtta 
      gtgtcagtg tctgtttat gcatttagt gtcaaatcg 
      tttttcgaa gaaaacata aaatcaaaa aaaaaaaca 
      aatatgttc aaaatcgtc ggaatattc caactccgg 
      aaattcgaa taaattctt aatgaattt attcaagtt 
      tcaagatta atcgcacgt tgtttagtt tgtaaaccc 
      acacattgt aaacaaatg taaaatctt catttgaaa 
      atgctttaa atctcataa tcacaaaaa tggagattc 
      tacatttcg tcattgatt tgaaagttg ttttgtaag 
      tgtataaga aatattttg gccgaatta actgtgtac 
      tattctgtg ttgatcatt ttgtattaa ataaacgaa 
      tgtaaataa gattgcaat aaaacctat atttaaaca 
      agtcaataa tatagaaac agtataaat gttaaccat 
      gtaatccgt atatatttc agtaaataa aacgtaaac 
      gtaaat
    • >TC032553 RB
      tggtgttgc tcttttcca tcagaaagc cgtcactcc 
      ctttttctt tcgtaaatg ttccgaaca tcgcccgat 
      agaaaatcg ctctctacg cgcgtttcc ccttcgaaa 
      caaaattgt tgtgcaaat gtaaataaa taaaattcc 
      cctcgcagc gttccgtca ccaaaaaca attttcatg 
      tttggcaaa atactcaat atgaatgtg gtggtcttc 
      ctgctgaat gtgctgatt gtggtggtg gtcagtggc 
      gggcccgct tggcgagaa gtccaaagg ggcggcggg 
      ctgagggtc ggctccggc gccggcaaa aactcctct 
      tcgtcgaga ggcaacgga attagggtc ggcggcagc 
      accagaggc gttcggatc accaccacg atgatgccg 
      gaaggcgac caagtcagc caaccgacc gctacggcc 
      acttccaac caccagcat acgcctctc aatgtgggg 
      atggtgctg ccgtccaag tccttcggg gtgagggag 
      tacaccaag gctgtcact tcggccaag tacaacttg 
      cagaggaaa ttacggctt ttcaagcac cacgatatc 
      aaggttcac atcgtgatg aaagaactc acccctagt 
      ccaacaggt aattcgatg gctggagat cataaggat 
      tcgcttaac gtcggactc attttgccg tacacgaat 
      ttcggggtc cgggaatac accagagcg atcaacaat 
      gcggtctct ggactacat cgatctagg ggacagcgc 
      ttaaattgg ctcaagaag tataatttc accccgaaa 
      aacgtccac tatgttctc attactctc acgcctagt 
      ccaacagca attctcaag tccctctgc aaggaattc 
      ctttcggtt aatgtgtca gccatttta tatttaatg 
      aattacgag aaatacggt cgaagtacg gcatctgca 
      cagtatttc cttcaatta gccggctac ttagggata 
      cctgtcatc gcctggaat gcggataat tcaggattg 
      gaaaggaga gcatcacaa tcttccctc caacttcaa 
      ttagcgcca tcgctggaa catcaaaca gctgcgatg 
      ttgagcata ttagagaga tataagtgg catcaattt 
      tcagtggta accagtccg atcgcagga cacgatgat 
      ttcatacag gctgtgcgg gagcgagtc tcggcaatg 
      caggaccgc ttcaagttt acgatcctc aacgcagtc 
      ctcgtatca caccatcgc gacttggct gcacttgtg 
      gattctgaa gcccgagta atgctgctt tattgtacg 
      agtcaagaa gcaatagac atacttact gccgctaag 
      gattttcac cttactgga gaaaattat gtttgggtt 
      gtaacacaa agcgttata gcgaaccca cttgaagca 
      ccaggccaa tttccagtt ggcatgtta ggtgtacat 
      tttgacaca tcgagttcc agtctggta aacgagata 
      acgaccgca atcaaagtg tatgcttat ggtgtggaa 
      gacttcaca aacgacctg gcaaatgca gggcgctcc 
      cttaatacc caactttcg tgcgaaggc gaaggtgca 
      gctcgatgg aataccggt gatcggttt tttcgggta 
      cttcgcaat gtgagtgtt gaaggcgaa gccgggaaa 
      cccaatctg gaattcacg caagacgga gtgttgaaa 
      gcggctgag ttgaaaata atgaatctt aggccagga 
      gttagcaaa cagctcgtt tgggaagag attggagtt 
      tggaagtcc tggcagaag gagggttta gacattaaa 
      gacattgtc tggccggga aatagccac acgccaccg 
      caaggagtt cctgagaag tttcatcta aaaatcact 
      tttttggaa gaaccacca tacataagc ctagcacct 
      cctgacccc gttacagga aagtgttca atggatagg 
      ggggtgttg tgcaggatt gccagcgac gcagacatc 
      accgaggtc gacacgacg ctggcccac cgcaacggc 
      agtttctat cagtgctgc tctggattt tgcatcgac 
      cttttgcaa aaattctcc gaagagttg ggtttcacc 
      tacgaactc gtccgtgtt gaagacggg agatgggga 
      acgaacgaa aatggaaag tggaacggc ctcattgct 
      gatctagtt aatcgaaaa acagatatg gttctgacg 
      tcgctgatg attaatgcg gaaagagag gcagtggta 
      gacttcagt gttcctttt atggagacg ggaattgca 
      atcgttgtg gccaaacga actggaatt atttctccg 
      acggcattt ttggaaccg tttgataca gcctcttgg 
      atgcttgtg ggggttgtc gcaattcaa gctgccacc 
      ttcaccatt ttccttttt gaatggctg tctccgagt 
      ggtttcaat atgagattg tcgcttaat cagtcaacg 
      ccatcagcg tcacatcgt ttttcgtta ttccgcact 
      tactggctg gtttgggct gtccttttc caagcggcg 
      gttcatgtt gattctccc agaggattc acagcacgt 
      tttatgaca aatgtttgg gcgatgttc gccgtagtc 
      ttccttgcc atatacact gccaacctc gcagccttc 
      atgattacc agagaagag ttttttgaa ttttcaggt 
      ctcgacgac caccgctta tcgcggcca tactcgcaa 
      aagccactc atcaaattc ggaacaatt ccctggtct 
      cacaccgat tcaacaata gcgaaatat ttcaaagaa 
      atgcacgct tacatgaga caattcaac aaatccaca 
      gtccatgaa ggagtcgac gccgtttta agcgccgaa 
      atggacgct ttcatatac gacggcact gtcttagat 
      tacctcact tcgcaagac gaagactgc agacttctc 
      accgtcggt tcctggtac gcaatgacc ggctacggc 
      ctggccttc ccccgaaat tccaaatac ctgaaaatg 
      ttcaacaaa cgactgctg gattttcga gaaaacggc 
      gaccttgaa cgtctgcgc cgctactgg atgaccggg 
      gtctgtaag cccggaaaa caggagcac aagagctcg 
      gacccctta gctttagaa cagttctta tcagcgttc 
      ctattgttg atggcgggg atactactc gccgcgctg 
      ctgttgttt ctcgaacac ttgtacttc aaatacgtt 
      cggaagcac ctcgcaaag accgaccga ggaggctgt 
      tgtgccctc atcagtctg tctatgggc aaaagtctg 
      acgtttcga ggggctgtg tacgaggct caagacatc 
      ctgagacat caccgctgc agagacccc atttgcgac 
      actcatctc tggaaagtc aaacgggag ttggacatc 
      tcccaaatg aggtgtaag cagctggag aaggagttg 
      gaagcgcac ggtataaag ccgccgcct ccatgcaaa 
      cgtgtaata gtttctgga gaacaagcc agatctagg 
      ttaagaagc ttaaatcca gataaaagt agcaactct 
      gatttattt ggtcagaag acagaaata gcagaaatg 
      gaaacagta ctgtgatca attaagaaa atcgagatg 
      aacaacacc aaataattt ccgagtaca ttggtacca 
      tcatcattg cctgaaaca aagaactct tatgaattg 
      tggtgcatt gttaaatag tatcagtga ctacctaac 
      acataccaa tacaaaact tcttggtgc cggataagc 
      tcataattc gtcaaatac aaatagcat ttagatcga 
      aatatttat tcatattgt agatagtca gtcgcaaat 
      tcgaccgtt atgacgtta aactgactg aacgttgta 
      tagattaca tttttatca aactcaaag actaatgtt 
      gcagaacaa attattttg tcgcccaac tgtaaccta 
      taccacttt ctattagtt tacattgtt gttgctata 
      atgttgttg ctgttattg ttgatgttt tcacgctat 
      aaattatgt caatatgat gtataataa aggtcatga 
      gaaatttca aaacggaaa ttttatcac acaccacca 
      ataaacccc aacacgtat cccggaaat aaaaccgta 
      gaatttagt tgtaaaaaa tttaagaga caatgttca 
      caagactgc ctttgttgc ataagattg caaactaaa 
      tttttaaat aaattcctc ctcgacttc tagtagaat 
      taaacaaca ttgaaaatt tctaatgac cgttcagaa 
      gtacacgac aggagtctg ttttttatg tttgtttag 
      aacaaatac tttcgactc atttgtagg aagcctact 
      ccttggcct aattctaga cagttttta acagatttg 
      taaatcgaa aaatgattt gacgcttaa atgatggct 
      tagttgatg acactgtta gccactaaa tggtaaatg 
      tcttgaatg catgtccat acaaaacac tcgaaacat 
      ttaataaaa tcaatgtta gggactaaa aaattcgtc 
      tcttaaatg tgtgtaaat attaataat tttacgtta 
      gtgtcagtg tctgtttat gcatttagt gtcaaatcg 
      tttttcgaa gaaaacata aaatcaaaa aaaaaaaca 
      aatatgttc aaaatcgtc ggaatattc caactccgg 
      aaattcgaa taaattctt aatgaattt attcaagtt 
      tcaagatta atcgcacgt tgtttagtt tgtaaaccc 
      acacattgt aaacaaatg taaaatctt catttgaaa 
      atgctttaa atctcataa tcacaaaaa tggagattc 
      tacatttcg tcattgatt tgaaagttg ttttgtaag 
      tgtataaga aatattttg gccgaatta actgtgtac 
      tattctgtg ttgatcatt ttgtattaa ataaacgaa 
      tgtaaataa gattgcaat aaaacctat atttaaaca 
      agtcaataa tatagaaac agtataaat gttaaccat 
      gtaatccgt atatatttc agtaaataa aacgtaaac 
      gtaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY