| General information | |||
| TC032553
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 16335589..16523932 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032553 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03624 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
remarks: later than schedule, hours 48 remarks: later than schedule, hours 48 remarks: later than schedule, hours 48
Pupal morphology
remarks: later than schedule, hours 48
Adult morphology
remarks: later than schedule, hours 48
remarks: later than schedule, hours 48
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
remarks: later than schedule, hours 48 (egglay)
remarks: later than schedule, hours 48
remarks: later than schedule, hours 48
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
remarks: later than schedule, hours 48
remarks - cuticles: later than schedule, hours 48
remarks: later than schedule, hours 48
remarks - cuticles: later than schedule, hours 48
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
- >TC032553 RA
MNVVVFLLN VLIVVVVSG GPAWREVQR GGGLRVGSG AGKNSSSSR GNGIRVGGS TRGVRITTT MMPEGDQVS QPTATATSN HQHTPLNVG MVLPSKSFG VREYTKAVT SAKYNLQRK LRLFKHHDI KVHIVMKEL TPSPTAILK SLCKEFLSV NVSAILYLM NYEKYGRST ASAQYFLQL AGYLGIPVI AWNADNSGL ERRASQSSL QLQLAPSLE HQTAAMLSI LERYKWHQF SVVTSPIAG HDDFIQAVR ERVSAMQDR FKFTILNAV LVSHHRDLA ALVDSEARV MLLYCTSQE AIDILTAAK DFHLTGENY VWVVTQSVI ANPLEAPGQ FPVGMLGVH FDTSSSSLV NEITTAIKV YAYGVEDFT NDLANAGRS LNTQLSCEG EGAARWNTG DRFFRVLRN VSVEGEAGK PNLEFTQDG VLKAAELKI MNLRPGVSK QLVWEEIGV WKSWQKEGL DIKDIVWPG NSHTPPQGV PEKFHLKIT FLEEPPYIS LAPPDPVTG KCSMDRGVL CRIASDADI TEVDTTLAH RNGSFYQCC SGFCIDLLQ KFSEELGFT YELVRVEDG RWGTNENGK WNGLIADLV NRKTDMVLT SLMINAERE AVVDFSVPF METGIAIVV AKRTGIISP TAFLEPFDT ASWMLVGVV AIQAATFTI FLFEWLSPS GFNMRLSLN QSTPSASHR FSLFRTYWL VWAVLFQAA VHVDSPRGF TARFMTNVW AMFAVVFLA IYTANLAAF MITREEFFE FSGLDDHRL SRPYSQKPL IKFGTIPWS HTDSTIAKY FKEMHAYMR QFNKSTVHE GVDAVLSAE MDAFIYDGT VLDYLTSQD EDCRLLTVG SWYAMTGYG LAFPRNSKY LKMFNKRLL DFRENGDLE RLRRYWMTG VCKPGKQEH KSSDPLALE QFLSAFLLL MAGILLAAL LLFLEHLYF KYVRKHLAK TDRGGCCAL ISLSMGKSL TFRGAVYEA QDILRHHRC RDPICDTHL WKVKRELDI SQMRCKQLE KELEAHGIK PPPPCKRVI VSGEQARSR LRSLNPDKS SNSDLFGQK TEIAEMETV L
- >TC032553 RB
MNVVVFLLN VLIVVVVSG GPAWREVQR GGGLRVGSG AGKNSSSSR GNGIRVGGS TRGVRITTT MMPEGDQVS QPTATATSN HQHTPLNVG MVLPSKSFG VREYTKAVT SAKYNLQRK LRLFKHHDI KVHIVMKEL TPSPTGNSM AGDHKDSLN VGLILPYTN FGVREYTRA INNAVSGLH RSRGQRLNW LKKYNFTPK NVHYVLITL TPSPTAILK SLCKEFLSV NVSAILYLM NYEKYGRST ASAQYFLQL AGYLGIPVI AWNADNSGL ERRASQSSL QLQLAPSLE HQTAAMLSI LERYKWHQF SVVTSPIAG HDDFIQAVR ERVSAMQDR FKFTILNAV LVSHHRDLA ALVDSEARV MLLYCTSQE AIDILTAAK DFHLTGENY VWVVTQSVI ANPLEAPGQ FPVGMLGVH FDTSSSSLV NEITTAIKV YAYGVEDFT NDLANAGRS LNTQLSCEG EGAARWNTG DRFFRVLRN VSVEGEAGK PNLEFTQDG VLKAAELKI MNLRPGVSK QLVWEEIGV WKSWQKEGL DIKDIVWPG NSHTPPQGV PEKFHLKIT FLEEPPYIS LAPPDPVTG KCSMDRGVL CRIASDADI TEVDTTLAH RNGSFYQCC SGFCIDLLQ KFSEELGFT YELVRVEDG RWGTNENGK WNGLIADLV NRKTDMVLT SLMINAERE AVVDFSVPF METGIAIVV AKRTGIISP TAFLEPFDT ASWMLVGVV AIQAATFTI FLFEWLSPS GFNMRLSLN QSTPSASHR FSLFRTYWL VWAVLFQAA VHVDSPRGF TARFMTNVW AMFAVVFLA IYTANLAAF MITREEFFE FSGLDDHRL SRPYSQKPL IKFGTIPWS HTDSTIAKY FKEMHAYMR QFNKSTVHE GVDAVLSAE MDAFIYDGT VLDYLTSQD EDCRLLTVG SWYAMTGYG LAFPRNSKY LKMFNKRLL DFRENGDLE RLRRYWMTG VCKPGKQEH KSSDPLALE QFLSAFLLL MAGILLAAL LLFLEHLYF KYVRKHLAK TDRGGCCAL ISLSMGKSL TFRGAVYEA QDILRHHRC RDPICDTHL WKVKRELDI SQMRCKQLE KELEAHGIK PPPPCKRVI VSGEQARSR LRSLNPDKS SNSDLFGQK TEIAEMETV L
- >TC032553 RA
atgaatgtg gtggtcttc ctgctgaat gtgctgatt gtggtggtg gtcagtggc gggcccgct tggcgagaa gtccaaagg ggcggcggg ctgagggtc ggctccggc gccggcaaa aactcctct tcgtcgaga ggcaacgga attagggtc ggcggcagc accagaggc gttcggatc accaccacg atgatgccg gaaggcgac caagtcagc caaccgacc gctacggcc acttccaac caccagcat acgcctctc aatgtgggg atggtgctg ccgtccaag tccttcggg gtgagggag tacaccaag gctgtcact tcggccaag tacaacttg cagaggaaa ttacggctt ttcaagcac cacgatatc aaggttcac atcgtgatg aaagaactc acccctagt ccaacagca attctcaag tccctctgc aaggaattc ctttcggtt aatgtgtca gccatttta tatttaatg aattacgag aaatacggt cgaagtacg gcatctgca cagtatttc cttcaatta gccggctac ttagggata cctgtcatc gcctggaat gcggataat tcaggattg gaaaggaga gcatcacaa tcttccctc caacttcaa ttagcgcca tcgctggaa catcaaaca gctgcgatg ttgagcata ttagagaga tataagtgg catcaattt tcagtggta accagtccg atcgcagga cacgatgat ttcatacag gctgtgcgg gagcgagtc tcggcaatg caggaccgc ttcaagttt acgatcctc aacgcagtc ctcgtatca caccatcgc gacttggct gcacttgtg gattctgaa gcccgagta atgctgctt tattgtacg agtcaagaa gcaatagac atacttact gccgctaag gattttcac cttactgga gaaaattat gtttgggtt gtaacacaa agcgttata gcgaaccca cttgaagca ccaggccaa tttccagtt ggcatgtta ggtgtacat tttgacaca tcgagttcc agtctggta aacgagata acgaccgca atcaaagtg tatgcttat ggtgtggaa gacttcaca aacgacctg gcaaatgca gggcgctcc cttaatacc caactttcg tgcgaaggc gaaggtgca gctcgatgg aataccggt gatcggttt tttcgggta cttcgcaat gtgagtgtt gaaggcgaa gccgggaaa cccaatctg gaattcacg caagacgga gtgttgaaa gcggctgag ttgaaaata atgaatctt aggccagga gttagcaaa cagctcgtt tgggaagag attggagtt tggaagtcc tggcagaag gagggttta gacattaaa gacattgtc tggccggga aatagccac acgccaccg caaggagtt cctgagaag tttcatcta aaaatcact tttttggaa gaaccacca tacataagc ctagcacct cctgacccc gttacagga aagtgttca atggatagg ggggtgttg tgcaggatt gccagcgac gcagacatc accgaggtc gacacgacg ctggcccac cgcaacggc agtttctat cagtgctgc tctggattt tgcatcgac cttttgcaa aaattctcc gaagagttg ggtttcacc tacgaactc gtccgtgtt gaagacggg agatgggga acgaacgaa aatggaaag tggaacggc ctcattgct gatctagtt aatcgaaaa acagatatg gttctgacg tcgctgatg attaatgcg gaaagagag gcagtggta gacttcagt gttcctttt atggagacg ggaattgca atcgttgtg gccaaacga actggaatt atttctccg acggcattt ttggaaccg tttgataca gcctcttgg atgcttgtg ggggttgtc gcaattcaa gctgccacc ttcaccatt ttccttttt gaatggctg tctccgagt ggtttcaat atgagattg tcgcttaat cagtcaacg ccatcagcg tcacatcgt ttttcgtta ttccgcact tactggctg gtttgggct gtccttttc caagcggcg gttcatgtt gattctccc agaggattc acagcacgt tttatgaca aatgtttgg gcgatgttc gccgtagtc ttccttgcc atatacact gccaacctc gcagccttc atgattacc agagaagag ttttttgaa ttttcaggt ctcgacgac caccgctta tcgcggcca tactcgcaa aagccactc atcaaattc ggaacaatt ccctggtct cacaccgat tcaacaata gcgaaatat ttcaaagaa atgcacgct tacatgaga caattcaac aaatccaca gtccatgaa ggagtcgac gccgtttta agcgccgaa atggacgct ttcatatac gacggcact gtcttagat tacctcact tcgcaagac gaagactgc agacttctc accgtcggt tcctggtac gcaatgacc ggctacggc ctggccttc ccccgaaat tccaaatac ctgaaaatg ttcaacaaa cgactgctg gattttcga gaaaacggc gaccttgaa cgtctgcgc cgctactgg atgaccggg gtctgtaag cccggaaaa caggagcac aagagctcg gacccctta gctttagaa cagttctta tcagcgttc ctattgttg atggcgggg atactactc gccgcgctg ctgttgttt ctcgaacac ttgtacttc aaatacgtt cggaagcac ctcgcaaag accgaccga ggaggctgt tgtgccctc atcagtctg tctatgggc aaaagtctg acgtttcga ggggctgtg tacgaggct caagacatc ctgagacat caccgctgc agagacccc atttgcgac actcatctc tggaaagtc aaacgggag ttggacatc tcccaaatg aggtgtaag cagctggag aaggagttg gaagcgcac ggtataaag ccgccgcct ccatgcaaa cgtgtaata gtttctgga gaacaagcc agatctagg ttaagaagc ttaaatcca gataaaagt agcaactct gatttattt ggtcagaag acagaaata gcagaaatg gaaacagta ctgtga
- >TC032553 RB
atgaatgtg gtggtcttc ctgctgaat gtgctgatt gtggtggtg gtcagtggc gggcccgct tggcgagaa gtccaaagg ggcggcggg ctgagggtc ggctccggc gccggcaaa aactcctct tcgtcgaga ggcaacgga attagggtc ggcggcagc accagaggc gttcggatc accaccacg atgatgccg gaaggcgac caagtcagc caaccgacc gctacggcc acttccaac caccagcat acgcctctc aatgtgggg atggtgctg ccgtccaag tccttcggg gtgagggag tacaccaag gctgtcact tcggccaag tacaacttg cagaggaaa ttacggctt ttcaagcac cacgatatc aaggttcac atcgtgatg aaagaactc acccctagt ccaacaggt aattcgatg gctggagat cataaggat tcgcttaac gtcggactc attttgccg tacacgaat ttcggggtc cgggaatac accagagcg atcaacaat gcggtctct ggactacat cgatctagg ggacagcgc ttaaattgg ctcaagaag tataatttc accccgaaa aacgtccac tatgttctc attactctc acgcctagt ccaacagca attctcaag tccctctgc aaggaattc ctttcggtt aatgtgtca gccatttta tatttaatg aattacgag aaatacggt cgaagtacg gcatctgca cagtatttc cttcaatta gccggctac ttagggata cctgtcatc gcctggaat gcggataat tcaggattg gaaaggaga gcatcacaa tcttccctc caacttcaa ttagcgcca tcgctggaa catcaaaca gctgcgatg ttgagcata ttagagaga tataagtgg catcaattt tcagtggta accagtccg atcgcagga cacgatgat ttcatacag gctgtgcgg gagcgagtc tcggcaatg caggaccgc ttcaagttt acgatcctc aacgcagtc ctcgtatca caccatcgc gacttggct gcacttgtg gattctgaa gcccgagta atgctgctt tattgtacg agtcaagaa gcaatagac atacttact gccgctaag gattttcac cttactgga gaaaattat gtttgggtt gtaacacaa agcgttata gcgaaccca cttgaagca ccaggccaa tttccagtt ggcatgtta ggtgtacat tttgacaca tcgagttcc agtctggta aacgagata acgaccgca atcaaagtg tatgcttat ggtgtggaa gacttcaca aacgacctg gcaaatgca gggcgctcc cttaatacc caactttcg tgcgaaggc gaaggtgca gctcgatgg aataccggt gatcggttt tttcgggta cttcgcaat gtgagtgtt gaaggcgaa gccgggaaa cccaatctg gaattcacg caagacgga gtgttgaaa gcggctgag ttgaaaata atgaatctt aggccagga gttagcaaa cagctcgtt tgggaagag attggagtt tggaagtcc tggcagaag gagggttta gacattaaa gacattgtc tggccggga aatagccac acgccaccg caaggagtt cctgagaag tttcatcta aaaatcact tttttggaa gaaccacca tacataagc ctagcacct cctgacccc gttacagga aagtgttca atggatagg ggggtgttg tgcaggatt gccagcgac gcagacatc accgaggtc gacacgacg ctggcccac cgcaacggc agtttctat cagtgctgc tctggattt tgcatcgac cttttgcaa aaattctcc gaagagttg ggtttcacc tacgaactc gtccgtgtt gaagacggg agatgggga acgaacgaa aatggaaag tggaacggc ctcattgct gatctagtt aatcgaaaa acagatatg gttctgacg tcgctgatg attaatgcg gaaagagag gcagtggta gacttcagt gttcctttt atggagacg ggaattgca atcgttgtg gccaaacga actggaatt atttctccg acggcattt ttggaaccg tttgataca gcctcttgg atgcttgtg ggggttgtc gcaattcaa gctgccacc ttcaccatt ttccttttt gaatggctg tctccgagt ggtttcaat atgagattg tcgcttaat cagtcaacg ccatcagcg tcacatcgt ttttcgtta ttccgcact tactggctg gtttgggct gtccttttc caagcggcg gttcatgtt gattctccc agaggattc acagcacgt tttatgaca aatgtttgg gcgatgttc gccgtagtc ttccttgcc atatacact gccaacctc gcagccttc atgattacc agagaagag ttttttgaa ttttcaggt ctcgacgac caccgctta tcgcggcca tactcgcaa aagccactc atcaaattc ggaacaatt ccctggtct cacaccgat tcaacaata gcgaaatat ttcaaagaa atgcacgct tacatgaga caattcaac aaatccaca gtccatgaa ggagtcgac gccgtttta agcgccgaa atggacgct ttcatatac gacggcact gtcttagat tacctcact tcgcaagac gaagactgc agacttctc accgtcggt tcctggtac gcaatgacc ggctacggc ctggccttc ccccgaaat tccaaatac ctgaaaatg ttcaacaaa cgactgctg gattttcga gaaaacggc gaccttgaa cgtctgcgc cgctactgg atgaccggg gtctgtaag cccggaaaa caggagcac aagagctcg gacccctta gctttagaa cagttctta tcagcgttc ctattgttg atggcgggg atactactc gccgcgctg ctgttgttt ctcgaacac ttgtacttc aaatacgtt cggaagcac ctcgcaaag accgaccga ggaggctgt tgtgccctc atcagtctg tctatgggc aaaagtctg acgtttcga ggggctgtg tacgaggct caagacatc ctgagacat caccgctgc agagacccc atttgcgac actcatctc tggaaagtc aaacgggag ttggacatc tcccaaatg aggtgtaag cagctggag aaggagttg gaagcgcac ggtataaag ccgccgcct ccatgcaaa cgtgtaata gtttctgga gaacaagcc agatctagg ttaagaagc ttaaatcca gataaaagt agcaactct gatttattt ggtcagaag acagaaata gcagaaatg gaaacagta ctgtga
- >TC032553 RA
tggtgttgc tcttttcca tcagaaagc cgtcactcc ctttttctt tcgtaaatg ttccgaaca tcgcccgat agaaaatcg ctctctacg cgcgtttcc ccttcgaaa caaaattgt tgtgcaaat gtaaataaa taaaattcc cctcgcagc gttccgtca ccaaaaaca attttcatg tttggcaaa atactcaat atgaatgtg gtggtcttc ctgctgaat gtgctgatt gtggtggtg gtcagtggc gggcccgct tggcgagaa gtccaaagg ggcggcggg ctgagggtc ggctccggc gccggcaaa aactcctct tcgtcgaga ggcaacgga attagggtc ggcggcagc accagaggc gttcggatc accaccacg atgatgccg gaaggcgac caagtcagc caaccgacc gctacggcc acttccaac caccagcat acgcctctc aatgtgggg atggtgctg ccgtccaag tccttcggg gtgagggag tacaccaag gctgtcact tcggccaag tacaacttg cagaggaaa ttacggctt ttcaagcac cacgatatc aaggttcac atcgtgatg aaagaactc acccctagt ccaacagca attctcaag tccctctgc aaggaattc ctttcggtt aatgtgtca gccatttta tatttaatg aattacgag aaatacggt cgaagtacg gcatctgca cagtatttc cttcaatta gccggctac ttagggata cctgtcatc gcctggaat gcggataat tcaggattg gaaaggaga gcatcacaa tcttccctc caacttcaa ttagcgcca tcgctggaa catcaaaca gctgcgatg ttgagcata ttagagaga tataagtgg catcaattt tcagtggta accagtccg atcgcagga cacgatgat ttcatacag gctgtgcgg gagcgagtc tcggcaatg caggaccgc ttcaagttt acgatcctc aacgcagtc ctcgtatca caccatcgc gacttggct gcacttgtg gattctgaa gcccgagta atgctgctt tattgtacg agtcaagaa gcaatagac atacttact gccgctaag gattttcac cttactgga gaaaattat gtttgggtt gtaacacaa agcgttata gcgaaccca cttgaagca ccaggccaa tttccagtt ggcatgtta ggtgtacat tttgacaca tcgagttcc agtctggta aacgagata acgaccgca atcaaagtg tatgcttat ggtgtggaa gacttcaca aacgacctg gcaaatgca gggcgctcc cttaatacc caactttcg tgcgaaggc gaaggtgca gctcgatgg aataccggt gatcggttt tttcgggta cttcgcaat gtgagtgtt gaaggcgaa gccgggaaa cccaatctg gaattcacg caagacgga gtgttgaaa gcggctgag ttgaaaata atgaatctt aggccagga gttagcaaa cagctcgtt tgggaagag attggagtt tggaagtcc tggcagaag gagggttta gacattaaa gacattgtc tggccggga aatagccac acgccaccg caaggagtt cctgagaag tttcatcta aaaatcact tttttggaa gaaccacca tacataagc ctagcacct cctgacccc gttacagga aagtgttca atggatagg ggggtgttg tgcaggatt gccagcgac gcagacatc accgaggtc gacacgacg ctggcccac cgcaacggc agtttctat cagtgctgc tctggattt tgcatcgac cttttgcaa aaattctcc gaagagttg ggtttcacc tacgaactc gtccgtgtt gaagacggg agatgggga acgaacgaa aatggaaag tggaacggc ctcattgct gatctagtt aatcgaaaa acagatatg gttctgacg tcgctgatg attaatgcg gaaagagag gcagtggta gacttcagt gttcctttt atggagacg ggaattgca atcgttgtg gccaaacga actggaatt atttctccg acggcattt ttggaaccg tttgataca gcctcttgg atgcttgtg ggggttgtc gcaattcaa gctgccacc ttcaccatt ttccttttt gaatggctg tctccgagt ggtttcaat atgagattg tcgcttaat cagtcaacg ccatcagcg tcacatcgt ttttcgtta ttccgcact tactggctg gtttgggct gtccttttc caagcggcg gttcatgtt gattctccc agaggattc acagcacgt tttatgaca aatgtttgg gcgatgttc gccgtagtc ttccttgcc atatacact gccaacctc gcagccttc atgattacc agagaagag ttttttgaa ttttcaggt ctcgacgac caccgctta tcgcggcca tactcgcaa aagccactc atcaaattc ggaacaatt ccctggtct cacaccgat tcaacaata gcgaaatat ttcaaagaa atgcacgct tacatgaga caattcaac aaatccaca gtccatgaa ggagtcgac gccgtttta agcgccgaa atggacgct ttcatatac gacggcact gtcttagat tacctcact tcgcaagac gaagactgc agacttctc accgtcggt tcctggtac gcaatgacc ggctacggc ctggccttc ccccgaaat tccaaatac ctgaaaatg ttcaacaaa cgactgctg gattttcga gaaaacggc gaccttgaa cgtctgcgc cgctactgg atgaccggg gtctgtaag cccggaaaa caggagcac aagagctcg gacccctta gctttagaa cagttctta tcagcgttc ctattgttg atggcgggg atactactc gccgcgctg ctgttgttt ctcgaacac ttgtacttc aaatacgtt cggaagcac ctcgcaaag accgaccga ggaggctgt tgtgccctc atcagtctg tctatgggc aaaagtctg acgtttcga ggggctgtg tacgaggct caagacatc ctgagacat caccgctgc agagacccc atttgcgac actcatctc tggaaagtc aaacgggag ttggacatc tcccaaatg aggtgtaag cagctggag aaggagttg gaagcgcac ggtataaag ccgccgcct ccatgcaaa cgtgtaata gtttctgga gaacaagcc agatctagg ttaagaagc ttaaatcca gataaaagt agcaactct gatttattt ggtcagaag acagaaata gcagaaatg gaaacagta ctgtgatca attaagaaa atcgagatg aacaacacc aaataattt ccgagtaca ttggtacca tcatcattg cctgaaaca aagaactct tatgaattg tggtgcatt gttaaatag tatcagtga ctacctaac acataccaa tacaaaact tcttggtgc cggataagc tcataattc gtcaaatac aaatagcat ttagatcga aatatttat tcatattgt agatagtca gtcgcaaat tcgaccgtt atgacgtta aactgactg aacgttgta tagattaca tttttatca aactcaaag actaatgtt gcagaacaa attattttg tcgcccaac tgtaaccta taccacttt ctattagtt tacattgtt gttgctata atgttgttg ctgttattg ttgatgttt tcacgctat aaattatgt caatatgat gtataataa aggtcatga gaaatttca aaacggaaa ttttatcac acaccacca ataaacccc aacacgtat cccggaaat aaaaccgta gaatttagt tgtaaaaaa tttaagaga caatgttca caagactgc ctttgttgc ataagattg caaactaaa tttttaaat aaattcctc ctcgacttc tagtagaat taaacaaca ttgaaaatt tctaatgac cgttcagaa gtacacgac aggagtctg ttttttatg tttgtttag aacaaatac tttcgactc atttgtagg aagcctact ccttggcct aattctaga cagttttta acagatttg taaatcgaa aaatgattt gacgcttaa atgatggct tagttgatg acactgtta gccactaaa tggtaaatg tcttgaatg catgtccat acaaaacac tcgaaacat ttaataaaa tcaatgtta gggactaaa aaattcgtc tcttaaatg tgtgtaaat attaataat tttacgtta gtgtcagtg tctgtttat gcatttagt gtcaaatcg tttttcgaa gaaaacata aaatcaaaa aaaaaaaca aatatgttc aaaatcgtc ggaatattc caactccgg aaattcgaa taaattctt aatgaattt attcaagtt tcaagatta atcgcacgt tgtttagtt tgtaaaccc acacattgt aaacaaatg taaaatctt catttgaaa atgctttaa atctcataa tcacaaaaa tggagattc tacatttcg tcattgatt tgaaagttg ttttgtaag tgtataaga aatattttg gccgaatta actgtgtac tattctgtg ttgatcatt ttgtattaa ataaacgaa tgtaaataa gattgcaat aaaacctat atttaaaca agtcaataa tatagaaac agtataaat gttaaccat gtaatccgt atatatttc agtaaataa aacgtaaac gtaaat
- >TC032553 RB
tggtgttgc tcttttcca tcagaaagc cgtcactcc ctttttctt tcgtaaatg ttccgaaca tcgcccgat agaaaatcg ctctctacg cgcgtttcc ccttcgaaa caaaattgt tgtgcaaat gtaaataaa taaaattcc cctcgcagc gttccgtca ccaaaaaca attttcatg tttggcaaa atactcaat atgaatgtg gtggtcttc ctgctgaat gtgctgatt gtggtggtg gtcagtggc gggcccgct tggcgagaa gtccaaagg ggcggcggg ctgagggtc ggctccggc gccggcaaa aactcctct tcgtcgaga ggcaacgga attagggtc ggcggcagc accagaggc gttcggatc accaccacg atgatgccg gaaggcgac caagtcagc caaccgacc gctacggcc acttccaac caccagcat acgcctctc aatgtgggg atggtgctg ccgtccaag tccttcggg gtgagggag tacaccaag gctgtcact tcggccaag tacaacttg cagaggaaa ttacggctt ttcaagcac cacgatatc aaggttcac atcgtgatg aaagaactc acccctagt ccaacaggt aattcgatg gctggagat cataaggat tcgcttaac gtcggactc attttgccg tacacgaat ttcggggtc cgggaatac accagagcg atcaacaat gcggtctct ggactacat cgatctagg ggacagcgc ttaaattgg ctcaagaag tataatttc accccgaaa aacgtccac tatgttctc attactctc acgcctagt ccaacagca attctcaag tccctctgc aaggaattc ctttcggtt aatgtgtca gccatttta tatttaatg aattacgag aaatacggt cgaagtacg gcatctgca cagtatttc cttcaatta gccggctac ttagggata cctgtcatc gcctggaat gcggataat tcaggattg gaaaggaga gcatcacaa tcttccctc caacttcaa ttagcgcca tcgctggaa catcaaaca gctgcgatg ttgagcata ttagagaga tataagtgg catcaattt tcagtggta accagtccg atcgcagga cacgatgat ttcatacag gctgtgcgg gagcgagtc tcggcaatg caggaccgc ttcaagttt acgatcctc aacgcagtc ctcgtatca caccatcgc gacttggct gcacttgtg gattctgaa gcccgagta atgctgctt tattgtacg agtcaagaa gcaatagac atacttact gccgctaag gattttcac cttactgga gaaaattat gtttgggtt gtaacacaa agcgttata gcgaaccca cttgaagca ccaggccaa tttccagtt ggcatgtta ggtgtacat tttgacaca tcgagttcc agtctggta aacgagata acgaccgca atcaaagtg tatgcttat ggtgtggaa gacttcaca aacgacctg gcaaatgca gggcgctcc cttaatacc caactttcg tgcgaaggc gaaggtgca gctcgatgg aataccggt gatcggttt tttcgggta cttcgcaat gtgagtgtt gaaggcgaa gccgggaaa cccaatctg gaattcacg caagacgga gtgttgaaa gcggctgag ttgaaaata atgaatctt aggccagga gttagcaaa cagctcgtt tgggaagag attggagtt tggaagtcc tggcagaag gagggttta gacattaaa gacattgtc tggccggga aatagccac acgccaccg caaggagtt cctgagaag tttcatcta aaaatcact tttttggaa gaaccacca tacataagc ctagcacct cctgacccc gttacagga aagtgttca atggatagg ggggtgttg tgcaggatt gccagcgac gcagacatc accgaggtc gacacgacg ctggcccac cgcaacggc agtttctat cagtgctgc tctggattt tgcatcgac cttttgcaa aaattctcc gaagagttg ggtttcacc tacgaactc gtccgtgtt gaagacggg agatgggga acgaacgaa aatggaaag tggaacggc ctcattgct gatctagtt aatcgaaaa acagatatg gttctgacg tcgctgatg attaatgcg gaaagagag gcagtggta gacttcagt gttcctttt atggagacg ggaattgca atcgttgtg gccaaacga actggaatt atttctccg acggcattt ttggaaccg tttgataca gcctcttgg atgcttgtg ggggttgtc gcaattcaa gctgccacc ttcaccatt ttccttttt gaatggctg tctccgagt ggtttcaat atgagattg tcgcttaat cagtcaacg ccatcagcg tcacatcgt ttttcgtta ttccgcact tactggctg gtttgggct gtccttttc caagcggcg gttcatgtt gattctccc agaggattc acagcacgt tttatgaca aatgtttgg gcgatgttc gccgtagtc ttccttgcc atatacact gccaacctc gcagccttc atgattacc agagaagag ttttttgaa ttttcaggt ctcgacgac caccgctta tcgcggcca tactcgcaa aagccactc atcaaattc ggaacaatt ccctggtct cacaccgat tcaacaata gcgaaatat ttcaaagaa atgcacgct tacatgaga caattcaac aaatccaca gtccatgaa ggagtcgac gccgtttta agcgccgaa atggacgct ttcatatac gacggcact gtcttagat tacctcact tcgcaagac gaagactgc agacttctc accgtcggt tcctggtac gcaatgacc ggctacggc ctggccttc ccccgaaat tccaaatac ctgaaaatg ttcaacaaa cgactgctg gattttcga gaaaacggc gaccttgaa cgtctgcgc cgctactgg atgaccggg gtctgtaag cccggaaaa caggagcac aagagctcg gacccctta gctttagaa cagttctta tcagcgttc ctattgttg atggcgggg atactactc gccgcgctg ctgttgttt ctcgaacac ttgtacttc aaatacgtt cggaagcac ctcgcaaag accgaccga ggaggctgt tgtgccctc atcagtctg tctatgggc aaaagtctg acgtttcga ggggctgtg tacgaggct caagacatc ctgagacat caccgctgc agagacccc atttgcgac actcatctc tggaaagtc aaacgggag ttggacatc tcccaaatg aggtgtaag cagctggag aaggagttg gaagcgcac ggtataaag ccgccgcct ccatgcaaa cgtgtaata gtttctgga gaacaagcc agatctagg ttaagaagc ttaaatcca gataaaagt agcaactct gatttattt ggtcagaag acagaaata gcagaaatg gaaacagta ctgtgatca attaagaaa atcgagatg aacaacacc aaataattt ccgagtaca ttggtacca tcatcattg cctgaaaca aagaactct tatgaattg tggtgcatt gttaaatag tatcagtga ctacctaac acataccaa tacaaaact tcttggtgc cggataagc tcataattc gtcaaatac aaatagcat ttagatcga aatatttat tcatattgt agatagtca gtcgcaaat tcgaccgtt atgacgtta aactgactg aacgttgta tagattaca tttttatca aactcaaag actaatgtt gcagaacaa attattttg tcgcccaac tgtaaccta taccacttt ctattagtt tacattgtt gttgctata atgttgttg ctgttattg ttgatgttt tcacgctat aaattatgt caatatgat gtataataa aggtcatga gaaatttca aaacggaaa ttttatcac acaccacca ataaacccc aacacgtat cccggaaat aaaaccgta gaatttagt tgtaaaaaa tttaagaga caatgttca caagactgc ctttgttgc ataagattg caaactaaa tttttaaat aaattcctc ctcgacttc tagtagaat taaacaaca ttgaaaatt tctaatgac cgttcagaa gtacacgac aggagtctg ttttttatg tttgtttag aacaaatac tttcgactc atttgtagg aagcctact ccttggcct aattctaga cagttttta acagatttg taaatcgaa aaatgattt gacgcttaa atgatggct tagttgatg acactgtta gccactaaa tggtaaatg tcttgaatg catgtccat acaaaacac tcgaaacat ttaataaaa tcaatgtta gggactaaa aaattcgtc tcttaaatg tgtgtaaat attaataat tttacgtta gtgtcagtg tctgtttat gcatttagt gtcaaatcg tttttcgaa gaaaacata aaatcaaaa aaaaaaaca aatatgttc aaaatcgtc ggaatattc caactccgg aaattcgaa taaattctt aatgaattt attcaagtt tcaagatta atcgcacgt tgtttagtt tgtaaaccc acacattgt aaacaaatg taaaatctt catttgaaa atgctttaa atctcataa tcacaaaaa tggagattc tacatttcg tcattgatt tgaaagttg ttttgtaag tgtataaga aatattttg gccgaatta actgtgtac tattctgtg ttgatcatt ttgtattaa ataaacgaa tgtaaataa gattgcaat aaaacctat atttaaaca agtcaataa tatagaaac agtataaat gttaaccat gtaatccgt atatatttc agtaaataa aacgtaaac gtaaat
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