Status
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Gene information
General information
TC032549
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003791,TC003792
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 17018270..17044486
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032549
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03621

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20
    only 6 eggs on the slide, all showing the annotated phenotype
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032549 RA
      MLSTNSMSV AINASTATS VAAAAVSVL QDNNSSQTI 
      NGGSANSQQ LDKSEINDA VTKVLQGYD WTLVPIASK 
      AASDKRKLH VKRPMNAFM VWAQAARRK LADQYPQLH 
      NAELSKTLG KLWRLLSDV DKKPFIEEA ERLRREHPD 
      YKYQPRRRK QNKSPGDSM LQLPHGQNV TFSRSLKQE 
      DSPCSPRSH SSTSPSTCS SQPHSPAIQ PQTLRSCLE 
      QHNLDFNRL SEIDNSYIS EDCIDNSDL DQYFPSETS 
      QSYQPSYPQ NYAKHPTEE ETNNNYKSK RLCTENIAH 
      QEAYEETAS SAGRYHELQ PSSVVKAER FVPPNASVM 
      YSYQSGAPI GSTSAYYTS SGHQYLPSY QYLPQRPVF 
      GNSSLTYNG ESNPEPWGH YTL
    CDS sequence
    • >TC032549 RA
      atgttatcg acgaattcg atgtcggtt gccatcaac 
      gcctcgacg gcgacgagc gtcgcggcg gccgccgtt 
      tcggttctc caagataac aattcttca cagacaatc 
      aacggtggc tcggccaac tcacaacaa ctcgataaa 
      agtgagatt aatgatgcg gttactaaa gttttgcaa 
      gggtatgac tggacgcta gtgcccatc gcatccaag 
      gctgcttcc gacaagcga aaactgcac gtgaaacgc 
      ccaatgaac gcttttatg gtgtgggcc caagccgcc 
      agacggaaa ttagctgat cagtatcca caactccac 
      aatgcggaa ctgtcaaaa acacttggc aagttatgg 
      agactactg agcgacgtg gacaagaaa cccttcatc 
      gaagaagcc gaacgcctc cgtcgcgaa caccccgac 
      tacaaatac caacctcgc cgaagaaaa cagaacaaa 
      agtcccggc gactccatg ctccaactc ccccacggc 
      caaaacgtc accttctcc cgctccctc aaacaggaa 
      gactcccct tgcagtccc cgcagccac agctccacc 
      tctccctcc acttgttcc tcgcaacct cacagtccc 
      gccatccaa ccccaaacc ctccgctcc tgtctggag 
      caacacaac ctggacttc aaccgactc tccgaaata 
      gacaatagt tacatatcg gaggattgt atcgacaac 
      agcgacctg gaccagtac ttcccctca gagaccagc 
      cagtcctac caacccagt tacccccag aactacgcc 
      aaacaccca acggaggaa gagaccaac aataactac 
      aagagcaag aggttgtgc acggagaac atcgcgcac 
      caggaggcg tacgaagaa actgcgtcg tctgctggg 
      aggtaccat gagctgcag ccgagctct gtggtcaaa 
      gctgaaaga tttgttcca cctaatgct tcggtaatg 
      tacagttac cagtcggga gcgcccatt ggttcgact 
      tccgcttat tacacgagt agtggccat cagtattta 
      ccttcgtac cagtatttg ccccaaagg cccgttttt 
      ggaaactcc tccctgact tacaacggt gagagtaat 
      cctgaaccg tggggacat tacacgctg tag
    mRNA sequence
    • >TC032549 RA
      cggagttag caacgtctt aagccacga ttgactcag 
      ccatctcgt ccttcttta ccctacaaa cggaaaaga 
      gacaacctc tacacactc gctcacatc tgcagcaag 
      tgcgtggtt taaagaaac agctctcag attttcaaa 
      gcactcttt gattggtgt cgtagaagg tgctagtgc 
      atgcaattc gaacaggac tttattgtt ttctaattg 
      tttgccgtc aggtggttg agattcatt cataaattt 
      cgcgtgcgt agatcagtg actgattat ttttgaaat 
      gttatcgac gaattcgat gtcggttgc catcaacgc 
      ctcgacggc gacgagcgt cgcggcggc cgccgtttc 
      ggttctcca agataacaa ttcttcaca gacaatcaa 
      cggtggctc ggccaactc acaacaact cgataaaag 
      tgagattaa tgatgcggt tactaaagt tttgcaagg 
      gtatgactg gacgctagt gcccatcgc atccaaggc 
      tgcttccga caagcgaaa actgcacgt gaaacgccc 
      aatgaacgc ttttatggt gtgggccca agccgccag 
      acggaaatt agctgatca gtatccaca actccacaa 
      tgcggaact gtcaaaaac acttggcaa gttatggag 
      actactgag cgacgtgga caagaaacc cttcatcga 
      agaagccga acgcctccg tcgcgaaca ccccgacta 
      caaatacca acctcgccg aagaaaaca gaacaaaag 
      tcccggcga ctccatgct ccaactccc ccacggcca 
      aaacgtcac cttctcccg ctccctcaa acaggaaga 
      ctccccttg cagtccccg cagccacag ctccacctc 
      tccctccac ttgttcctc gcaacctca cagtcccgc 
      catccaacc ccaaaccct ccgctcctg tctggagca 
      acacaacct ggacttcaa ccgactctc cgaaataga 
      caatagtta catatcgga ggattgtat cgacaacag 
      cgacctgga ccagtactt cccctcaga gaccagcca 
      gtcctacca acccagtta cccccagaa ctacgccaa 
      acacccaac ggaggaaga gaccaacaa taactacaa 
      gagcaagag gttgtgcac ggagaacat cgcgcacca 
      ggaggcgta cgaagaaac tgcgtcgtc tgctgggag 
      gtaccatga gctgcagcc gagctctgt ggtcaaagc 
      tgaaagatt tgttccacc taatgcttc ggtaatgta 
      cagttacca gtcgggagc gcccattgg ttcgacttc 
      cgcttatta cacgagtag tggccatca gtatttacc 
      ttcgtacca gtatttgcc ccaaaggcc cgtttttgg 
      aaactcctc cctgactta caacggtga gagtaatcc 
      tgaaccgtg gggacatta cacgctgta gtctgactg 
      aaacttgtt gcaaaacgt gatatttaa gttgctttg 
      tattttatt tgatgtaag tacttacaa tattttatt 
      actttatta ttgtagaca aaaatgttt ctcttgaag 
      ccaaagaaa tcatttaat cacaaccaa gagaaacgt 
      ttgttaagt tattctaag cgtctaggt aggtcttgt 
      tttctatga taccatgct ctgttttaa gtgcatgtt 
      tgtacttat aaaataagt gccttaggt ccgaatatt 
      tttatataa aattagcta attacatag agtccttta 
      tatccaaat aaacgtgtc gaatataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY