| General information | |||
| TC032545
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 17172406..17340208 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC032545 | ||
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03620 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
- >TC032545 RA
MITHETSFT DRAALALRL RLTTFAAVY VYSLLSTVF GEDSFALPP SFALGSNLE VSEYDLLAA VKIPFQDPK TQYIDEDGH DGFPALGFK AGSDVKIAH RQILPEKLP AEFSILISA KPRSRKGGF IFAVVNPYD TVVGLGVRI APEGTSNTV ISLFYTDTS HIASQIIAN FTVNRFNGK WTRFAFRLS VDNVTLFFN CNETETVPV KREPLELIF DSASTLYIA QAGPVIGEP YEGALDQVK IFRDPNMAA DQCKTDFQG FQKLNPENE NEIDEVERS DYDGTFREG SGSEDYGFI PPPPPPPNG KPCICDNDH CCNYLFNDI KASLGNKGD KGDRGPRGP PGESIRGPP GPPGPPGLP GPSAPEGVC SCNFTSLIS SLSSSNLLG VSEGKPGPP GMPGQPGLP GDKGIPGIP GDKGERGER GPQGPIGLQ GPKGEPGKD GLPGKPGAP GPPGPPGPV EFENVDETV LGGPLVRPG VPGPKGEPG KPGPSGPRG EKGPTGTKG LRGDPGPKG ERGDRGFPG ERGPPGVKG DTGSPGLDG IPGNPGKHG DKGEKGAVG PPGPPGIAY NSDMTGIVP GLPGPKGEP GEKGPKGDK GEEGEGGIP GVPGMKGEP GGPGQKGEQ GESGAIGPV GPPGSKGER GPPGSIIMP NGNGEIVTV KGDKGDMGR RGRRGKPGP PGPAGPPGK SGPMGEIGL PGWMGRPGA SGLPGISGP KGEKGDPGG YGGPKGDKG DKGDRGQDG IPGRDGRDG SPGPPGLPG AASSDETIR YVPVPGPPG PPGPPGIPG VSVTGPKGE PGGPPYGEP TYNLRPGRS TASPPIFTV SEQEVAARI VPGAVTFQD HDAMIKMSS VSPVGTLAY VIEEEALLV RVNNGWQYI ALGSVLPVT TPSPPTTNS PQLRPPFEA SNLINHISK PADVPNVSN KFETMIEHL
- >TC032545 RB
MITHETSFT DRAALALRL RLTTFAAVY VYSLLSTVF GEDSFALPP SFALGSNLE VSEYDLLAA VKIPFQDPK TQYIDEDGH DGFPALGFK AGSDVKIAH RQILPEKLP AEFSILISA KPRSRKGGF IFAVVNPYD TVVGLGVRI APEGTSNTV ISLFYTDTS HIASQIIAN FTVNRFNGK WTRFAFRLS VDNVTLFFN CNETETVPV KREPLELIF DSASTLYIA QAGPVIGEP YEGALDQVK IFRDPNMAA DQCKTDFQG FQKLNPENE NEIDEVERS DYDGTFREG SGSEDYGFI PPPPPPPNG KPCICDNDH CCNYLFNDI KASLGNKGD KGDRGPRGP PGESIRGPP GPPGPPGLP GPSAPEGVC SCNFTSLIS SLSSSNLLG VSEGKPGPP GMPGQPGLP GDKGIPGIP GDKGERGER GPQGPIGLQ GPKGEPGKD GLPGKPGAP GPPGPPGPV EFENVDETV LGGPLVRPG VPGPKGEPG KPGPSGPRG EKGPTGTKG LRGDPGPKG ERGDRGFPG ERGPPGVKG DTGSPGLDG IPGNPGKHG DKGEKGAVG PPGPPGIAY NSDMTGIVP GLPGPKGEP GEKGPKGDK GEEGEGGIP GVPGMKGEP GGPGQKGEQ GESGAIGPV GPPGSKGER GPPGSIIMP NGNGEIVTV KGDKGDMGR RGRRGKPGP PGPAGPPGK SGPMGEIGL PGWMGRPGA SGLPGISGP KGEKGDPGG YGGPKGDKG DKGDRGQDG IPGRDGRDG SPGPPGLPG AASSDETIR YVPVPGPPG PPGPPGIPG VSVTGPKGE PGGPPYGEP TYNLRPGTR SSLEELKAL RELKDLKSG RSTASPPIF TVSEQEVAA RIVPGAVTF QDHDAMIKM SSVSPVGTL AYVIEEEAL LVRVNNGWQ YIALGSVLP VTTPSPPTT NSPQLRPPF EASNLINHI SKPADVPNV SNKFETMIE HL
- >TC032545 RA
atgattact catgaaaca tccttcaca gatagggcg gccctagca cttcgtcta agattaaca acgtttgca gcagtttac gtctattca ttattatca acagtattc ggcgaggat tcatttgct ttgccccca tcttttgca cttgggagt aatttagaa gtatcagaa tacgatctg cttgcggct gtgaaaatt ccgttccaa gatccgaag actcagtac atcgacgag gacggccat gacggattt cccgctctc ggcttcaaa gccggatcc gacgtgaaa atagctcac cgccagata ttgccggaa aaactgccg gcggaattt tcgatttta attagtgcg aaaccgcga tccagaaag ggcggattt atatttgcc gtagtcaac ccatacgat actgttgtg ggattggga gtaagaatt gctccggag ggaacgagt aataccgtc atatcttta ttttacacg gacacttct catattgcc tcccagatt atcgcaaat tttactgtt aatagattc aacggaaaa tggacgcgc ttcgctttc cgcttatcc gtcgacaat gtcacatta ttttttaac tgtaacgag acggaaacc gttcccgtc aagcgggaa ccattagag ttaattttc gattccgct tccacgcta tatattgca caagccgga cctgtcatc ggggagccc tacgagggc gccctggac caagtgaaa atattccgc gaccctaac atggctgca gatcaatgt aaaacagat tttcagggt tttcaaaag ctcaatccc gaaaacgaa aacgaaata gatgaagtc gaacgcagc gactacgac ggaacattc agagaagga agtggatcc gaagactac ggtttcatt ccacctcca cccccaccc ccaaatggt aagccttgc atttgtgat aacgaccat tgttgcaac tatcttttc aatgacatc aaagctagc ctaggaaat aagggcgat aaaggcgac agagggcca aggggccct cctggcgaa tcaataaga ggacctccg ggtcctcca ggcccccct ggcttgccg ggaccttca gctcccgag ggggtctgc tcttgtaac tttactagt ttaataagt tccttgtca tcatcaaac ttattggga gtcagtgag ggcaaacct ggccctcct ggtatgcca ggccagcca gggcttcct ggcgataaa ggtatccct ggtattccg ggtgataaa ggtgagagg ggtgagcgt ggtcctcaa ggaccgatc ggattgcaa ggacctaag ggtgaacct ggcaaggat ggactacct ggaaaacca ggcgctcct ggccctccg ggaccccct ggtccagtc gaattcgaa aatgttgac gaaacagtc cttgggggc cctctggtt cgtcctggc gtcccgggg cctaagggc gagcccggg aaacccggc ccttccggc cctcgaggc gaaaagggt cccacagga acaaaggga ttgcgcggt gatcctggc cctaaaggc gaacgcggc gatcgagga tttcctgga gaacgaggt cccccagga gttaaggga gacaccgga agtccgggt ttggacgga atacccgga aatcccgga aagcatggc gataagggc gaaaagggg gctgtagga ccgccaggc cctcccggg attgcgtat aatagtgac atgactggg attgtaccg ggattgcca ggcccgaag ggcgaaccg ggcgagaaa gggccgaag ggggataag ggcgaagag ggggagggt ggaataccg ggtgttccg ggaatgaaa ggggaacct ggtgggcct ggacagaag ggggagcag ggggaaagt ggggctatt gggccggtg ggaccgcca gggtccaag ggggaaagg gggccgcct gggagtatt attatgccg aatggaaat ggagaaatt gtaacagtg aagggcgat aaaggggat atgggaaga cgaggaaga aggggcaag cccggccca ccaggacca gcaggacct cccggaaaa tcaggccct atgggagaa attggacta cctggatgg atgggtcga ccgggagct tcaggatta ccgggaatt tcggggcca aagggtgaa aaaggcgac ccaggcggt tatggaggg cctaaaggt gataagggc gacaaaggt gatagaggc caagatgga attcctgga agagatggg agagacggc agtccgggt cctccgggt ttacctgga gcggcttct tcagacgaa acaatcaga tatgtgcca gtgccgggc ccgccgggg ccacctgga ccgcccgga attcccgga gtttcggta acaggacca aaaggagaa cctggcggt ccgccgtat ggagaaccg acatacaac ttacgacca ggcagatcc acagcaagt ccacctata ttcacggtt tctgagcaa gaagttgcg gctcgcatt gtgcccgga gctgtaact ttccaggac catgacgcc atgataaag atgtcgtcc gtcagtccc gtgggaact ttggcctat gttattgaa gaagaagcg cttcttgtg cgagttaat aacggctgg caatacatt gcgctggga tctgttctg cctgtgacc accccctcg ccacccacc acaaacagc ccacaactg aggccgcct ttcgaagcc agtaatctc attaatcac atatcgaaa ccagctgat gttcccaat gttagcaac aaatttgaa accatgatt gagcaccta tga
- >TC032545 RB
atgattact catgaaaca tccttcaca gatagggcg gccctagca cttcgtcta agattaaca acgtttgca gcagtttac gtctattca ttattatca acagtattc ggcgaggat tcatttgct ttgccccca tcttttgca cttgggagt aatttagaa gtatcagaa tacgatctg cttgcggct gtgaaaatt ccgttccaa gatccgaag actcagtac atcgacgag gacggccat gacggattt cccgctctc ggcttcaaa gccggatcc gacgtgaaa atagctcac cgccagata ttgccggaa aaactgccg gcggaattt tcgatttta attagtgcg aaaccgcga tccagaaag ggcggattt atatttgcc gtagtcaac ccatacgat actgttgtg ggattggga gtaagaatt gctccggag ggaacgagt aataccgtc atatcttta ttttacacg gacacttct catattgcc tcccagatt atcgcaaat tttactgtt aatagattc aacggaaaa tggacgcgc ttcgctttc cgcttatcc gtcgacaat gtcacatta ttttttaac tgtaacgag acggaaacc gttcccgtc aagcgggaa ccattagag ttaattttc gattccgct tccacgcta tatattgca caagccgga cctgtcatc ggggagccc tacgagggc gccctggac caagtgaaa atattccgc gaccctaac atggctgca gatcaatgt aaaacagat tttcagggt tttcaaaag ctcaatccc gaaaacgaa aacgaaata gatgaagtc gaacgcagc gactacgac ggaacattc agagaagga agtggatcc gaagactac ggtttcatt ccacctcca cccccaccc ccaaatggt aagccttgc atttgtgat aacgaccat tgttgcaac tatcttttc aatgacatc aaagctagc ctaggaaat aagggcgat aaaggcgac agagggcca aggggccct cctggcgaa tcaataaga ggacctccg ggtcctcca ggcccccct ggcttgccg ggaccttca gctcccgag ggggtctgc tcttgtaac tttactagt ttaataagt tccttgtca tcatcaaac ttattggga gtcagtgag ggcaaacct ggccctcct ggtatgcca ggccagcca gggcttcct ggcgataaa ggtatccct ggtattccg ggtgataaa ggtgagagg ggtgagcgt ggtcctcaa ggaccgatc ggattgcaa ggacctaag ggtgaacct ggcaaggat ggactacct ggaaaacca ggcgctcct ggccctccg ggaccccct ggtccagtc gaattcgaa aatgttgac gaaacagtc cttgggggc cctctggtt cgtcctggc gtcccgggg cctaagggc gagcccggg aaacccggc ccttccggc cctcgaggc gaaaagggt cccacagga acaaaggga ttgcgcggt gatcctggc cctaaaggc gaacgcggc gatcgagga tttcctgga gaacgaggt cccccagga gttaaggga gacaccgga agtccgggt ttggacgga atacccgga aatcccgga aagcatggc gataagggc gaaaagggg gctgtagga ccgccaggc cctcccggg attgcgtat aatagtgac atgactggg attgtaccg ggattgcca ggcccgaag ggcgaaccg ggcgagaaa gggccgaag ggggataag ggcgaagag ggggagggt ggaataccg ggtgttccg ggaatgaaa ggggaacct ggtgggcct ggacagaag ggggagcag ggggaaagt ggggctatt gggccggtg ggaccgcca gggtccaag ggggaaagg gggccgcct gggagtatt attatgccg aatggaaat ggagaaatt gtaacagtg aagggcgat aaaggggat atgggaaga cgaggaaga aggggcaag cccggccca ccaggacca gcaggacct cccggaaaa tcaggccct atgggagaa attggacta cctggatgg atgggtcga ccgggagct tcaggatta ccgggaatt tcggggcca aagggtgaa aaaggcgac ccaggcggt tatggaggg cctaaaggt gataagggc gacaaaggt gatagaggc caagatgga attcctgga agagatggg agagacggc agtccgggt cctccgggt ttacctgga gcggcttct tcagacgaa acaatcaga tatgtgcca gtgccgggc ccgccgggg ccacctgga ccgcccgga attcccgga gtttcggta acaggacca aaaggagaa cctggcggt ccgccgtat ggagaaccg acatacaac ttacgacca ggaacaaga agtagtttg gaggaattg aaggctctc agagaactg aaggatttg aaatcaggc agatccaca gcaagtcca cctatattc acggtttct gagcaagaa gttgcggct cgcattgtg cccggagct gtaactttc caggaccat gacgccatg ataaagatg tcgtccgtc agtcccgtg ggaactttg gcctatgtt attgaagaa gaagcgctt cttgtgcga gttaataac ggctggcaa tacattgcg ctgggatct gttctgcct gtgaccacc ccctcgcca cccaccaca aacagccca caactgagg ccgcctttc gaagccagt aatctcatt aatcacata tcgaaacca gctgatgtt cccaatgtt agcaacaaa tttgaaacc atgattgag cacctatga
- >TC032545 RA
cgactttac aaaacgtta cgttgtcta tgattactc atgaaacat ccttcacag atagggcgg ccctagcac ttcgtctaa gattaacaa cgtttgcag cagtttacg tctattcat tattatcaa cagtattcg gcgaggatt catttgctt tgcccccat cttttgcac ttgggagta atttagaag tatcagaat acgatctgc ttgcggctg tgaaaattc cgttccaag atccgaaga ctcagtaca tcgacgagg acggccatg acggatttc ccgctctcg gcttcaaag ccggatccg acgtgaaaa tagctcacc gccagatat tgccggaaa aactgccgg cggaatttt cgattttaa ttagtgcga aaccgcgat ccagaaagg gcggattta tatttgccg tagtcaacc catacgata ctgttgtgg gattgggag taagaattg ctccggagg gaacgagta ataccgtca tatctttat tttacacgg acacttctc atattgcct cccagatta tcgcaaatt ttactgtta atagattca acggaaaat ggacgcgct tcgctttcc gcttatccg tcgacaatg tcacattat tttttaact gtaacgaga cggaaaccg ttcccgtca agcgggaac cattagagt taattttcg attccgctt ccacgctat atattgcac aagccggac ctgtcatcg gggagccct acgagggcg ccctggacc aagtgaaaa tattccgcg accctaaca tggctgcag atcaatgta aaacagatt ttcagggtt ttcaaaagc tcaatcccg aaaacgaaa acgaaatag atgaagtcg aacgcagcg actacgacg gaacattca gagaaggaa gtggatccg aagactacg gtttcattc cacctccac ccccacccc caaatggta agccttgca tttgtgata acgaccatt gttgcaact atcttttca atgacatca aagctagcc taggaaata agggcgata aaggcgaca gagggccaa ggggccctc ctggcgaat caataagag gacctccgg gtcctccag gcccccctg gcttgccgg gaccttcag ctcccgagg gggtctgct cttgtaact ttactagtt taataagtt ccttgtcat catcaaact tattgggag tcagtgagg gcaaacctg gccctcctg gtatgccag gccagccag ggcttcctg gcgataaag gtatccctg gtattccgg gtgataaag gtgagaggg gtgagcgtg gtcctcaag gaccgatcg gattgcaag gacctaagg gtgaacctg gcaaggatg gactacctg gaaaaccag gcgctcctg gccctccgg gaccccctg gtccagtcg aattcgaaa atgttgacg aaacagtcc ttgggggcc ctctggttc gtcctggcg tcccggggc ctaagggcg agcccggga aacccggcc cttccggcc ctcgaggcg aaaagggtc ccacaggaa caaagggat tgcgcggtg atcctggcc ctaaaggcg aacgcggcg atcgaggat ttcctggag aacgaggtc ccccaggag ttaagggag acaccggaa gtccgggtt tggacggaa tacccggaa atcccggaa agcatggcg ataagggcg aaaaggggg ctgtaggac cgccaggcc ctcccggga ttgcgtata atagtgaca tgactggga ttgtaccgg gattgccag gcccgaagg gcgaaccgg gcgagaaag ggccgaagg gggataagg gcgaagagg gggagggtg gaataccgg gtgttccgg gaatgaaag gggaacctg gtgggcctg gacagaagg gggagcagg gggaaagtg gggctattg ggccggtgg gaccgccag ggtccaagg gggaaaggg ggccgcctg ggagtatta ttatgccga atggaaatg gagaaattg taacagtga agggcgata aaggggata tgggaagac gaggaagaa ggggcaagc ccggcccac caggaccag caggacctc ccggaaaat caggcccta tgggagaaa ttggactac ctggatgga tgggtcgac cgggagctt caggattac cgggaattt cggggccaa agggtgaaa aaggcgacc caggcggtt atggagggc ctaaaggtg ataagggcg acaaaggtg atagaggcc aagatggaa ttcctggaa gagatggga gagacggca gtccgggtc ctccgggtt tacctggag cggcttctt cagacgaaa caatcagat atgtgccag tgccgggcc cgccggggc cacctggac cgcccggaa ttcccggag tttcggtaa caggaccaa aaggagaac ctggcggtc cgccgtatg gagaaccga catacaact tacgaccag gcagatcca cagcaagtc cacctatat tcacggttt ctgagcaag aagttgcgg ctcgcattg tgcccggag ctgtaactt tccaggacc atgacgcca tgataaaga tgtcgtccg tcagtcccg tgggaactt tggcctatg ttattgaag aagaagcgc ttcttgtgc gagttaata acggctggc aatacattg cgctgggat ctgttctgc ctgtgacca ccccctcgc cacccacca caaacagcc cacaactga ggccgcctt tcgaagcca gtaatctca ttaatcaca tatcgaaac cagctgatg ttcccaatg ttagcaaca aatttgaaa ccatgattg agcacctat gacctacaa atacagaac cggatttag atgtaattt tgcatgata tgccgattt gacctttaa ttgttttaa tttaaataa aatgtttaa aaatag
- >TC032545 RB
cgactttac aaaacgtta cgttgtcta tgattactc atgaaacat ccttcacag atagggcgg ccctagcac ttcgtctaa gattaacaa cgtttgcag cagtttacg tctattcat tattatcaa cagtattcg gcgaggatt catttgctt tgcccccat cttttgcac ttgggagta atttagaag tatcagaat acgatctgc ttgcggctg tgaaaattc cgttccaag atccgaaga ctcagtaca tcgacgagg acggccatg acggatttc ccgctctcg gcttcaaag ccggatccg acgtgaaaa tagctcacc gccagatat tgccggaaa aactgccgg cggaatttt cgattttaa ttagtgcga aaccgcgat ccagaaagg gcggattta tatttgccg tagtcaacc catacgata ctgttgtgg gattgggag taagaattg ctccggagg gaacgagta ataccgtca tatctttat tttacacgg acacttctc atattgcct cccagatta tcgcaaatt ttactgtta atagattca acggaaaat ggacgcgct tcgctttcc gcttatccg tcgacaatg tcacattat tttttaact gtaacgaga cggaaaccg ttcccgtca agcgggaac cattagagt taattttcg attccgctt ccacgctat atattgcac aagccggac ctgtcatcg gggagccct acgagggcg ccctggacc aagtgaaaa tattccgcg accctaaca tggctgcag atcaatgta aaacagatt ttcagggtt ttcaaaagc tcaatcccg aaaacgaaa acgaaatag atgaagtcg aacgcagcg actacgacg gaacattca gagaaggaa gtggatccg aagactacg gtttcattc cacctccac ccccacccc caaatggta agccttgca tttgtgata acgaccatt gttgcaact atcttttca atgacatca aagctagcc taggaaata agggcgata aaggcgaca gagggccaa ggggccctc ctggcgaat caataagag gacctccgg gtcctccag gcccccctg gcttgccgg gaccttcag ctcccgagg gggtctgct cttgtaact ttactagtt taataagtt ccttgtcat catcaaact tattgggag tcagtgagg gcaaacctg gccctcctg gtatgccag gccagccag ggcttcctg gcgataaag gtatccctg gtattccgg gtgataaag gtgagaggg gtgagcgtg gtcctcaag gaccgatcg gattgcaag gacctaagg gtgaacctg gcaaggatg gactacctg gaaaaccag gcgctcctg gccctccgg gaccccctg gtccagtcg aattcgaaa atgttgacg aaacagtcc ttgggggcc ctctggttc gtcctggcg tcccggggc ctaagggcg agcccggga aacccggcc cttccggcc ctcgaggcg aaaagggtc ccacaggaa caaagggat tgcgcggtg atcctggcc ctaaaggcg aacgcggcg atcgaggat ttcctggag aacgaggtc ccccaggag ttaagggag acaccggaa gtccgggtt tggacggaa tacccggaa atcccggaa agcatggcg ataagggcg aaaaggggg ctgtaggac cgccaggcc ctcccggga ttgcgtata atagtgaca tgactggga ttgtaccgg gattgccag gcccgaagg gcgaaccgg gcgagaaag ggccgaagg gggataagg gcgaagagg gggagggtg gaataccgg gtgttccgg gaatgaaag gggaacctg gtgggcctg gacagaagg gggagcagg gggaaagtg gggctattg ggccggtgg gaccgccag ggtccaagg gggaaaggg ggccgcctg ggagtatta ttatgccga atggaaatg gagaaattg taacagtga agggcgata aaggggata tgggaagac gaggaagaa ggggcaagc ccggcccac caggaccag caggacctc ccggaaaat caggcccta tgggagaaa ttggactac ctggatgga tgggtcgac cgggagctt caggattac cgggaattt cggggccaa agggtgaaa aaggcgacc caggcggtt atggagggc ctaaaggtg ataagggcg acaaaggtg atagaggcc aagatggaa ttcctggaa gagatggga gagacggca gtccgggtc ctccgggtt tacctggag cggcttctt cagacgaaa caatcagat atgtgccag tgccgggcc cgccggggc cacctggac cgcccggaa ttcccggag tttcggtaa caggaccaa aaggagaac ctggcggtc cgccgtatg gagaaccga catacaact tacgaccag gaacaagaa gtagtttgg aggaattga aggctctca gagaactga aggatttga aatcaggca gatccacag caagtccac ctatattca cggtttctg agcaagaag ttgcggctc gcattgtgc ccggagctg taactttcc aggaccatg acgccatga taaagatgt cgtccgtca gtcccgtgg gaactttgg cctatgtta ttgaagaag aagcgcttc ttgtgcgag ttaataacg gctggcaat acattgcgc tgggatctg ttctgcctg tgaccaccc cctcgccac ccaccacaa acagcccac aactgaggc cgcctttcg aagccagta atctcatta atcacatat cgaaaccag ctgatgttc ccaatgtta gcaacaaat ttgaaacca tgattgagc acctatgac ctacaaata cagaaccgg atttagatg taattttgc atgatatgc cgatttgac ctttaattg ttttaattt aaataaaat gtttaaaaa tag
