Status
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Gene information
General information
TC032545
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003786,TC003785
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 17172406..17340208
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032545
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03620

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032545 RA
      MITHETSFT DRAALALRL RLTTFAAVY VYSLLSTVF 
      GEDSFALPP SFALGSNLE VSEYDLLAA VKIPFQDPK 
      TQYIDEDGH DGFPALGFK AGSDVKIAH RQILPEKLP 
      AEFSILISA KPRSRKGGF IFAVVNPYD TVVGLGVRI 
      APEGTSNTV ISLFYTDTS HIASQIIAN FTVNRFNGK 
      WTRFAFRLS VDNVTLFFN CNETETVPV KREPLELIF 
      DSASTLYIA QAGPVIGEP YEGALDQVK IFRDPNMAA 
      DQCKTDFQG FQKLNPENE NEIDEVERS DYDGTFREG 
      SGSEDYGFI PPPPPPPNG KPCICDNDH CCNYLFNDI 
      KASLGNKGD KGDRGPRGP PGESIRGPP GPPGPPGLP 
      GPSAPEGVC SCNFTSLIS SLSSSNLLG VSEGKPGPP 
      GMPGQPGLP GDKGIPGIP GDKGERGER GPQGPIGLQ 
      GPKGEPGKD GLPGKPGAP GPPGPPGPV EFENVDETV 
      LGGPLVRPG VPGPKGEPG KPGPSGPRG EKGPTGTKG 
      LRGDPGPKG ERGDRGFPG ERGPPGVKG DTGSPGLDG 
      IPGNPGKHG DKGEKGAVG PPGPPGIAY NSDMTGIVP 
      GLPGPKGEP GEKGPKGDK GEEGEGGIP GVPGMKGEP 
      GGPGQKGEQ GESGAIGPV GPPGSKGER GPPGSIIMP 
      NGNGEIVTV KGDKGDMGR RGRRGKPGP PGPAGPPGK 
      SGPMGEIGL PGWMGRPGA SGLPGISGP KGEKGDPGG 
      YGGPKGDKG DKGDRGQDG IPGRDGRDG SPGPPGLPG 
      AASSDETIR YVPVPGPPG PPGPPGIPG VSVTGPKGE 
      PGGPPYGEP TYNLRPGRS TASPPIFTV SEQEVAARI 
      VPGAVTFQD HDAMIKMSS VSPVGTLAY VIEEEALLV 
      RVNNGWQYI ALGSVLPVT TPSPPTTNS PQLRPPFEA 
      SNLINHISK PADVPNVSN KFETMIEHL
    • >TC032545 RB
      MITHETSFT DRAALALRL RLTTFAAVY VYSLLSTVF 
      GEDSFALPP SFALGSNLE VSEYDLLAA VKIPFQDPK 
      TQYIDEDGH DGFPALGFK AGSDVKIAH RQILPEKLP 
      AEFSILISA KPRSRKGGF IFAVVNPYD TVVGLGVRI 
      APEGTSNTV ISLFYTDTS HIASQIIAN FTVNRFNGK 
      WTRFAFRLS VDNVTLFFN CNETETVPV KREPLELIF 
      DSASTLYIA QAGPVIGEP YEGALDQVK IFRDPNMAA 
      DQCKTDFQG FQKLNPENE NEIDEVERS DYDGTFREG 
      SGSEDYGFI PPPPPPPNG KPCICDNDH CCNYLFNDI 
      KASLGNKGD KGDRGPRGP PGESIRGPP GPPGPPGLP 
      GPSAPEGVC SCNFTSLIS SLSSSNLLG VSEGKPGPP 
      GMPGQPGLP GDKGIPGIP GDKGERGER GPQGPIGLQ 
      GPKGEPGKD GLPGKPGAP GPPGPPGPV EFENVDETV 
      LGGPLVRPG VPGPKGEPG KPGPSGPRG EKGPTGTKG 
      LRGDPGPKG ERGDRGFPG ERGPPGVKG DTGSPGLDG 
      IPGNPGKHG DKGEKGAVG PPGPPGIAY NSDMTGIVP 
      GLPGPKGEP GEKGPKGDK GEEGEGGIP GVPGMKGEP 
      GGPGQKGEQ GESGAIGPV GPPGSKGER GPPGSIIMP 
      NGNGEIVTV KGDKGDMGR RGRRGKPGP PGPAGPPGK 
      SGPMGEIGL PGWMGRPGA SGLPGISGP KGEKGDPGG 
      YGGPKGDKG DKGDRGQDG IPGRDGRDG SPGPPGLPG 
      AASSDETIR YVPVPGPPG PPGPPGIPG VSVTGPKGE 
      PGGPPYGEP TYNLRPGTR SSLEELKAL RELKDLKSG 
      RSTASPPIF TVSEQEVAA RIVPGAVTF QDHDAMIKM 
      SSVSPVGTL AYVIEEEAL LVRVNNGWQ YIALGSVLP 
      VTTPSPPTT NSPQLRPPF EASNLINHI SKPADVPNV 
      SNKFETMIE HL
    CDS sequence
    • >TC032545 RA
      atgattact catgaaaca tccttcaca gatagggcg 
      gccctagca cttcgtcta agattaaca acgtttgca 
      gcagtttac gtctattca ttattatca acagtattc 
      ggcgaggat tcatttgct ttgccccca tcttttgca 
      cttgggagt aatttagaa gtatcagaa tacgatctg 
      cttgcggct gtgaaaatt ccgttccaa gatccgaag 
      actcagtac atcgacgag gacggccat gacggattt 
      cccgctctc ggcttcaaa gccggatcc gacgtgaaa 
      atagctcac cgccagata ttgccggaa aaactgccg 
      gcggaattt tcgatttta attagtgcg aaaccgcga 
      tccagaaag ggcggattt atatttgcc gtagtcaac 
      ccatacgat actgttgtg ggattggga gtaagaatt 
      gctccggag ggaacgagt aataccgtc atatcttta 
      ttttacacg gacacttct catattgcc tcccagatt 
      atcgcaaat tttactgtt aatagattc aacggaaaa 
      tggacgcgc ttcgctttc cgcttatcc gtcgacaat 
      gtcacatta ttttttaac tgtaacgag acggaaacc 
      gttcccgtc aagcgggaa ccattagag ttaattttc 
      gattccgct tccacgcta tatattgca caagccgga 
      cctgtcatc ggggagccc tacgagggc gccctggac 
      caagtgaaa atattccgc gaccctaac atggctgca 
      gatcaatgt aaaacagat tttcagggt tttcaaaag 
      ctcaatccc gaaaacgaa aacgaaata gatgaagtc 
      gaacgcagc gactacgac ggaacattc agagaagga 
      agtggatcc gaagactac ggtttcatt ccacctcca 
      cccccaccc ccaaatggt aagccttgc atttgtgat 
      aacgaccat tgttgcaac tatcttttc aatgacatc 
      aaagctagc ctaggaaat aagggcgat aaaggcgac 
      agagggcca aggggccct cctggcgaa tcaataaga 
      ggacctccg ggtcctcca ggcccccct ggcttgccg 
      ggaccttca gctcccgag ggggtctgc tcttgtaac 
      tttactagt ttaataagt tccttgtca tcatcaaac 
      ttattggga gtcagtgag ggcaaacct ggccctcct 
      ggtatgcca ggccagcca gggcttcct ggcgataaa 
      ggtatccct ggtattccg ggtgataaa ggtgagagg 
      ggtgagcgt ggtcctcaa ggaccgatc ggattgcaa 
      ggacctaag ggtgaacct ggcaaggat ggactacct 
      ggaaaacca ggcgctcct ggccctccg ggaccccct 
      ggtccagtc gaattcgaa aatgttgac gaaacagtc 
      cttgggggc cctctggtt cgtcctggc gtcccgggg 
      cctaagggc gagcccggg aaacccggc ccttccggc 
      cctcgaggc gaaaagggt cccacagga acaaaggga 
      ttgcgcggt gatcctggc cctaaaggc gaacgcggc 
      gatcgagga tttcctgga gaacgaggt cccccagga 
      gttaaggga gacaccgga agtccgggt ttggacgga 
      atacccgga aatcccgga aagcatggc gataagggc 
      gaaaagggg gctgtagga ccgccaggc cctcccggg 
      attgcgtat aatagtgac atgactggg attgtaccg 
      ggattgcca ggcccgaag ggcgaaccg ggcgagaaa 
      gggccgaag ggggataag ggcgaagag ggggagggt 
      ggaataccg ggtgttccg ggaatgaaa ggggaacct 
      ggtgggcct ggacagaag ggggagcag ggggaaagt 
      ggggctatt gggccggtg ggaccgcca gggtccaag 
      ggggaaagg gggccgcct gggagtatt attatgccg 
      aatggaaat ggagaaatt gtaacagtg aagggcgat 
      aaaggggat atgggaaga cgaggaaga aggggcaag 
      cccggccca ccaggacca gcaggacct cccggaaaa 
      tcaggccct atgggagaa attggacta cctggatgg 
      atgggtcga ccgggagct tcaggatta ccgggaatt 
      tcggggcca aagggtgaa aaaggcgac ccaggcggt 
      tatggaggg cctaaaggt gataagggc gacaaaggt 
      gatagaggc caagatgga attcctgga agagatggg 
      agagacggc agtccgggt cctccgggt ttacctgga 
      gcggcttct tcagacgaa acaatcaga tatgtgcca 
      gtgccgggc ccgccgggg ccacctgga ccgcccgga 
      attcccgga gtttcggta acaggacca aaaggagaa 
      cctggcggt ccgccgtat ggagaaccg acatacaac 
      ttacgacca ggcagatcc acagcaagt ccacctata 
      ttcacggtt tctgagcaa gaagttgcg gctcgcatt 
      gtgcccgga gctgtaact ttccaggac catgacgcc 
      atgataaag atgtcgtcc gtcagtccc gtgggaact 
      ttggcctat gttattgaa gaagaagcg cttcttgtg 
      cgagttaat aacggctgg caatacatt gcgctggga 
      tctgttctg cctgtgacc accccctcg ccacccacc 
      acaaacagc ccacaactg aggccgcct ttcgaagcc 
      agtaatctc attaatcac atatcgaaa ccagctgat 
      gttcccaat gttagcaac aaatttgaa accatgatt 
      gagcaccta tga
    • >TC032545 RB
      atgattact catgaaaca tccttcaca gatagggcg 
      gccctagca cttcgtcta agattaaca acgtttgca 
      gcagtttac gtctattca ttattatca acagtattc 
      ggcgaggat tcatttgct ttgccccca tcttttgca 
      cttgggagt aatttagaa gtatcagaa tacgatctg 
      cttgcggct gtgaaaatt ccgttccaa gatccgaag 
      actcagtac atcgacgag gacggccat gacggattt 
      cccgctctc ggcttcaaa gccggatcc gacgtgaaa 
      atagctcac cgccagata ttgccggaa aaactgccg 
      gcggaattt tcgatttta attagtgcg aaaccgcga 
      tccagaaag ggcggattt atatttgcc gtagtcaac 
      ccatacgat actgttgtg ggattggga gtaagaatt 
      gctccggag ggaacgagt aataccgtc atatcttta 
      ttttacacg gacacttct catattgcc tcccagatt 
      atcgcaaat tttactgtt aatagattc aacggaaaa 
      tggacgcgc ttcgctttc cgcttatcc gtcgacaat 
      gtcacatta ttttttaac tgtaacgag acggaaacc 
      gttcccgtc aagcgggaa ccattagag ttaattttc 
      gattccgct tccacgcta tatattgca caagccgga 
      cctgtcatc ggggagccc tacgagggc gccctggac 
      caagtgaaa atattccgc gaccctaac atggctgca 
      gatcaatgt aaaacagat tttcagggt tttcaaaag 
      ctcaatccc gaaaacgaa aacgaaata gatgaagtc 
      gaacgcagc gactacgac ggaacattc agagaagga 
      agtggatcc gaagactac ggtttcatt ccacctcca 
      cccccaccc ccaaatggt aagccttgc atttgtgat 
      aacgaccat tgttgcaac tatcttttc aatgacatc 
      aaagctagc ctaggaaat aagggcgat aaaggcgac 
      agagggcca aggggccct cctggcgaa tcaataaga 
      ggacctccg ggtcctcca ggcccccct ggcttgccg 
      ggaccttca gctcccgag ggggtctgc tcttgtaac 
      tttactagt ttaataagt tccttgtca tcatcaaac 
      ttattggga gtcagtgag ggcaaacct ggccctcct 
      ggtatgcca ggccagcca gggcttcct ggcgataaa 
      ggtatccct ggtattccg ggtgataaa ggtgagagg 
      ggtgagcgt ggtcctcaa ggaccgatc ggattgcaa 
      ggacctaag ggtgaacct ggcaaggat ggactacct 
      ggaaaacca ggcgctcct ggccctccg ggaccccct 
      ggtccagtc gaattcgaa aatgttgac gaaacagtc 
      cttgggggc cctctggtt cgtcctggc gtcccgggg 
      cctaagggc gagcccggg aaacccggc ccttccggc 
      cctcgaggc gaaaagggt cccacagga acaaaggga 
      ttgcgcggt gatcctggc cctaaaggc gaacgcggc 
      gatcgagga tttcctgga gaacgaggt cccccagga 
      gttaaggga gacaccgga agtccgggt ttggacgga 
      atacccgga aatcccgga aagcatggc gataagggc 
      gaaaagggg gctgtagga ccgccaggc cctcccggg 
      attgcgtat aatagtgac atgactggg attgtaccg 
      ggattgcca ggcccgaag ggcgaaccg ggcgagaaa 
      gggccgaag ggggataag ggcgaagag ggggagggt 
      ggaataccg ggtgttccg ggaatgaaa ggggaacct 
      ggtgggcct ggacagaag ggggagcag ggggaaagt 
      ggggctatt gggccggtg ggaccgcca gggtccaag 
      ggggaaagg gggccgcct gggagtatt attatgccg 
      aatggaaat ggagaaatt gtaacagtg aagggcgat 
      aaaggggat atgggaaga cgaggaaga aggggcaag 
      cccggccca ccaggacca gcaggacct cccggaaaa 
      tcaggccct atgggagaa attggacta cctggatgg 
      atgggtcga ccgggagct tcaggatta ccgggaatt 
      tcggggcca aagggtgaa aaaggcgac ccaggcggt 
      tatggaggg cctaaaggt gataagggc gacaaaggt 
      gatagaggc caagatgga attcctgga agagatggg 
      agagacggc agtccgggt cctccgggt ttacctgga 
      gcggcttct tcagacgaa acaatcaga tatgtgcca 
      gtgccgggc ccgccgggg ccacctgga ccgcccgga 
      attcccgga gtttcggta acaggacca aaaggagaa 
      cctggcggt ccgccgtat ggagaaccg acatacaac 
      ttacgacca ggaacaaga agtagtttg gaggaattg 
      aaggctctc agagaactg aaggatttg aaatcaggc 
      agatccaca gcaagtcca cctatattc acggtttct 
      gagcaagaa gttgcggct cgcattgtg cccggagct 
      gtaactttc caggaccat gacgccatg ataaagatg 
      tcgtccgtc agtcccgtg ggaactttg gcctatgtt 
      attgaagaa gaagcgctt cttgtgcga gttaataac 
      ggctggcaa tacattgcg ctgggatct gttctgcct 
      gtgaccacc ccctcgcca cccaccaca aacagccca 
      caactgagg ccgcctttc gaagccagt aatctcatt 
      aatcacata tcgaaacca gctgatgtt cccaatgtt 
      agcaacaaa tttgaaacc atgattgag cacctatga
    mRNA sequence
    • >TC032545 RA
      cgactttac aaaacgtta cgttgtcta tgattactc 
      atgaaacat ccttcacag atagggcgg ccctagcac 
      ttcgtctaa gattaacaa cgtttgcag cagtttacg 
      tctattcat tattatcaa cagtattcg gcgaggatt 
      catttgctt tgcccccat cttttgcac ttgggagta 
      atttagaag tatcagaat acgatctgc ttgcggctg 
      tgaaaattc cgttccaag atccgaaga ctcagtaca 
      tcgacgagg acggccatg acggatttc ccgctctcg 
      gcttcaaag ccggatccg acgtgaaaa tagctcacc 
      gccagatat tgccggaaa aactgccgg cggaatttt 
      cgattttaa ttagtgcga aaccgcgat ccagaaagg 
      gcggattta tatttgccg tagtcaacc catacgata 
      ctgttgtgg gattgggag taagaattg ctccggagg 
      gaacgagta ataccgtca tatctttat tttacacgg 
      acacttctc atattgcct cccagatta tcgcaaatt 
      ttactgtta atagattca acggaaaat ggacgcgct 
      tcgctttcc gcttatccg tcgacaatg tcacattat 
      tttttaact gtaacgaga cggaaaccg ttcccgtca 
      agcgggaac cattagagt taattttcg attccgctt 
      ccacgctat atattgcac aagccggac ctgtcatcg 
      gggagccct acgagggcg ccctggacc aagtgaaaa 
      tattccgcg accctaaca tggctgcag atcaatgta 
      aaacagatt ttcagggtt ttcaaaagc tcaatcccg 
      aaaacgaaa acgaaatag atgaagtcg aacgcagcg 
      actacgacg gaacattca gagaaggaa gtggatccg 
      aagactacg gtttcattc cacctccac ccccacccc 
      caaatggta agccttgca tttgtgata acgaccatt 
      gttgcaact atcttttca atgacatca aagctagcc 
      taggaaata agggcgata aaggcgaca gagggccaa 
      ggggccctc ctggcgaat caataagag gacctccgg 
      gtcctccag gcccccctg gcttgccgg gaccttcag 
      ctcccgagg gggtctgct cttgtaact ttactagtt 
      taataagtt ccttgtcat catcaaact tattgggag 
      tcagtgagg gcaaacctg gccctcctg gtatgccag 
      gccagccag ggcttcctg gcgataaag gtatccctg 
      gtattccgg gtgataaag gtgagaggg gtgagcgtg 
      gtcctcaag gaccgatcg gattgcaag gacctaagg 
      gtgaacctg gcaaggatg gactacctg gaaaaccag 
      gcgctcctg gccctccgg gaccccctg gtccagtcg 
      aattcgaaa atgttgacg aaacagtcc ttgggggcc 
      ctctggttc gtcctggcg tcccggggc ctaagggcg 
      agcccggga aacccggcc cttccggcc ctcgaggcg 
      aaaagggtc ccacaggaa caaagggat tgcgcggtg 
      atcctggcc ctaaaggcg aacgcggcg atcgaggat 
      ttcctggag aacgaggtc ccccaggag ttaagggag 
      acaccggaa gtccgggtt tggacggaa tacccggaa 
      atcccggaa agcatggcg ataagggcg aaaaggggg 
      ctgtaggac cgccaggcc ctcccggga ttgcgtata 
      atagtgaca tgactggga ttgtaccgg gattgccag 
      gcccgaagg gcgaaccgg gcgagaaag ggccgaagg 
      gggataagg gcgaagagg gggagggtg gaataccgg 
      gtgttccgg gaatgaaag gggaacctg gtgggcctg 
      gacagaagg gggagcagg gggaaagtg gggctattg 
      ggccggtgg gaccgccag ggtccaagg gggaaaggg 
      ggccgcctg ggagtatta ttatgccga atggaaatg 
      gagaaattg taacagtga agggcgata aaggggata 
      tgggaagac gaggaagaa ggggcaagc ccggcccac 
      caggaccag caggacctc ccggaaaat caggcccta 
      tgggagaaa ttggactac ctggatgga tgggtcgac 
      cgggagctt caggattac cgggaattt cggggccaa 
      agggtgaaa aaggcgacc caggcggtt atggagggc 
      ctaaaggtg ataagggcg acaaaggtg atagaggcc 
      aagatggaa ttcctggaa gagatggga gagacggca 
      gtccgggtc ctccgggtt tacctggag cggcttctt 
      cagacgaaa caatcagat atgtgccag tgccgggcc 
      cgccggggc cacctggac cgcccggaa ttcccggag 
      tttcggtaa caggaccaa aaggagaac ctggcggtc 
      cgccgtatg gagaaccga catacaact tacgaccag 
      gcagatcca cagcaagtc cacctatat tcacggttt 
      ctgagcaag aagttgcgg ctcgcattg tgcccggag 
      ctgtaactt tccaggacc atgacgcca tgataaaga 
      tgtcgtccg tcagtcccg tgggaactt tggcctatg 
      ttattgaag aagaagcgc ttcttgtgc gagttaata 
      acggctggc aatacattg cgctgggat ctgttctgc 
      ctgtgacca ccccctcgc cacccacca caaacagcc 
      cacaactga ggccgcctt tcgaagcca gtaatctca 
      ttaatcaca tatcgaaac cagctgatg ttcccaatg 
      ttagcaaca aatttgaaa ccatgattg agcacctat 
      gacctacaa atacagaac cggatttag atgtaattt 
      tgcatgata tgccgattt gacctttaa ttgttttaa 
      tttaaataa aatgtttaa aaatag
    • >TC032545 RB
      cgactttac aaaacgtta cgttgtcta tgattactc 
      atgaaacat ccttcacag atagggcgg ccctagcac 
      ttcgtctaa gattaacaa cgtttgcag cagtttacg 
      tctattcat tattatcaa cagtattcg gcgaggatt 
      catttgctt tgcccccat cttttgcac ttgggagta 
      atttagaag tatcagaat acgatctgc ttgcggctg 
      tgaaaattc cgttccaag atccgaaga ctcagtaca 
      tcgacgagg acggccatg acggatttc ccgctctcg 
      gcttcaaag ccggatccg acgtgaaaa tagctcacc 
      gccagatat tgccggaaa aactgccgg cggaatttt 
      cgattttaa ttagtgcga aaccgcgat ccagaaagg 
      gcggattta tatttgccg tagtcaacc catacgata 
      ctgttgtgg gattgggag taagaattg ctccggagg 
      gaacgagta ataccgtca tatctttat tttacacgg 
      acacttctc atattgcct cccagatta tcgcaaatt 
      ttactgtta atagattca acggaaaat ggacgcgct 
      tcgctttcc gcttatccg tcgacaatg tcacattat 
      tttttaact gtaacgaga cggaaaccg ttcccgtca 
      agcgggaac cattagagt taattttcg attccgctt 
      ccacgctat atattgcac aagccggac ctgtcatcg 
      gggagccct acgagggcg ccctggacc aagtgaaaa 
      tattccgcg accctaaca tggctgcag atcaatgta 
      aaacagatt ttcagggtt ttcaaaagc tcaatcccg 
      aaaacgaaa acgaaatag atgaagtcg aacgcagcg 
      actacgacg gaacattca gagaaggaa gtggatccg 
      aagactacg gtttcattc cacctccac ccccacccc 
      caaatggta agccttgca tttgtgata acgaccatt 
      gttgcaact atcttttca atgacatca aagctagcc 
      taggaaata agggcgata aaggcgaca gagggccaa 
      ggggccctc ctggcgaat caataagag gacctccgg 
      gtcctccag gcccccctg gcttgccgg gaccttcag 
      ctcccgagg gggtctgct cttgtaact ttactagtt 
      taataagtt ccttgtcat catcaaact tattgggag 
      tcagtgagg gcaaacctg gccctcctg gtatgccag 
      gccagccag ggcttcctg gcgataaag gtatccctg 
      gtattccgg gtgataaag gtgagaggg gtgagcgtg 
      gtcctcaag gaccgatcg gattgcaag gacctaagg 
      gtgaacctg gcaaggatg gactacctg gaaaaccag 
      gcgctcctg gccctccgg gaccccctg gtccagtcg 
      aattcgaaa atgttgacg aaacagtcc ttgggggcc 
      ctctggttc gtcctggcg tcccggggc ctaagggcg 
      agcccggga aacccggcc cttccggcc ctcgaggcg 
      aaaagggtc ccacaggaa caaagggat tgcgcggtg 
      atcctggcc ctaaaggcg aacgcggcg atcgaggat 
      ttcctggag aacgaggtc ccccaggag ttaagggag 
      acaccggaa gtccgggtt tggacggaa tacccggaa 
      atcccggaa agcatggcg ataagggcg aaaaggggg 
      ctgtaggac cgccaggcc ctcccggga ttgcgtata 
      atagtgaca tgactggga ttgtaccgg gattgccag 
      gcccgaagg gcgaaccgg gcgagaaag ggccgaagg 
      gggataagg gcgaagagg gggagggtg gaataccgg 
      gtgttccgg gaatgaaag gggaacctg gtgggcctg 
      gacagaagg gggagcagg gggaaagtg gggctattg 
      ggccggtgg gaccgccag ggtccaagg gggaaaggg 
      ggccgcctg ggagtatta ttatgccga atggaaatg 
      gagaaattg taacagtga agggcgata aaggggata 
      tgggaagac gaggaagaa ggggcaagc ccggcccac 
      caggaccag caggacctc ccggaaaat caggcccta 
      tgggagaaa ttggactac ctggatgga tgggtcgac 
      cgggagctt caggattac cgggaattt cggggccaa 
      agggtgaaa aaggcgacc caggcggtt atggagggc 
      ctaaaggtg ataagggcg acaaaggtg atagaggcc 
      aagatggaa ttcctggaa gagatggga gagacggca 
      gtccgggtc ctccgggtt tacctggag cggcttctt 
      cagacgaaa caatcagat atgtgccag tgccgggcc 
      cgccggggc cacctggac cgcccggaa ttcccggag 
      tttcggtaa caggaccaa aaggagaac ctggcggtc 
      cgccgtatg gagaaccga catacaact tacgaccag 
      gaacaagaa gtagtttgg aggaattga aggctctca 
      gagaactga aggatttga aatcaggca gatccacag 
      caagtccac ctatattca cggtttctg agcaagaag 
      ttgcggctc gcattgtgc ccggagctg taactttcc 
      aggaccatg acgccatga taaagatgt cgtccgtca 
      gtcccgtgg gaactttgg cctatgtta ttgaagaag 
      aagcgcttc ttgtgcgag ttaataacg gctggcaat 
      acattgcgc tgggatctg ttctgcctg tgaccaccc 
      cctcgccac ccaccacaa acagcccac aactgaggc 
      cgcctttcg aagccagta atctcatta atcacatat 
      cgaaaccag ctgatgttc ccaatgtta gcaacaaat 
      ttgaaacca tgattgagc acctatgac ctacaaata 
      cagaaccgg atttagatg taattttgc atgatatgc 
      cgatttgac ctttaattg ttttaattt aaataaaat 
      gtttaaaaa tag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY