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Gene information
General information
TC003774 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 17786420..17797079
Sequence information
Transcripts / Proteins (4) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003774
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03618

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003774 RA
      MSVVGFGLV KIFLGSRHQ TPETADERV 
      SDLPPRHFG GPRGRGARR DNRRDERRR 
      TTDDEETVL DLTEVSSVD RESISSAEA 
      TWSGGRSQR RRRRRGRQR SLTPVINDT 
      TVNNGQAGP AGDVVPNDS HMTDGGANQ 
      KPRGGGDPK NRSNRPPPA RRNNEPKPK 
      EALVNGTTA
    • >TC003774 RB
      MSVVGFGLV KIFLGSRHQ TPETADERV 
      SDLPPRHFG GPRGRGARR DNRRDERRR 
      TTDDEETVL DLTEVSSVD RVNNGQAGP 
      AGDVVPNDS HMTDGGANQ KPRGGGDPK 
      NRSNRPPPA RRNNEPKPK EALVNGTTA
    • >TC003774 RC
      MEDLAVEVC GENGALYKG YVVDVFEDS VLIHFEDEW 
      QPDSKFPFS QVRLPPKPD PKVEFTENM EVEVYSRAN 
      HQEAYGWWK SRIKMMKGD FYVLEYVGW DTTYTEIVS 
      DDRLRVKNS NPPIDSSMF VKFEIEVPE DVREYAKIE 
      NAHKEFQNA IGASLIRYV PEKGVLVVI SRNESSRRC 
      ARLVQDMHF RSLSQKVLL LKRTEEAAR QLESTKLAT 
      IGGMLFYFV RERKFVHVK SSRGVFGVF CSCVDDAIF 
      FCRFSDEFN VREDLMGLA IGAHGANIQ QARKVDGIT 
      NIELEENSC TFKIYGETD EAVKKARSM LEYSEESLQ 
      VPRALVGKV IGKNGRIIQ EIVDKSGVV RVKIEGDNE 
      PQPTIPREE GQVPFVFVG TVESISNAK VLLKYHLAH 
      LKEVEQLRQ EKLEIDQQL RSIHGNALG SMQSLSMSR 
      RNDRGYNSD MDGGGRPGR GSMRGRGGR GRGGGGPGG 
      RQNDRYNSG TSTITDYVN NVDKRNNAA NKPMGNGRG 
      GGRGGGGGG HASSNGRPP RGGGGPPTN NRERPNKGS 
      RHQTPETAD ERVSDLPPR HFGGPRGRG ARRDNRRDE 
      RRRTTDDEE TVLDLTEVS SVDRESISS AEATWSGGR 
      SQRRRRRRG RQRSLTPVI NDTTVNNGQ AGPAGDVVP 
      NDSHMTDGG ANQKPRGGG DPKNRSNRP PPARRNNEP 
      KPKEALVNG TTA
    • >TC003774 RD
      MEDLAVEVC GENGALYKG YVVDVFEDS VLIHFEDEW 
      QPDSKFPFS QVRLPPKPD PKVEFTENM EVEVYSRAN 
      HQEAYGWWK SRIKMMKGD FYVLEYVGW DTTYTEIVS 
      DDRLRVKNS NPPIDSSMF VKFEIEVPE DVREYAKIE 
      NAHKEFQNA IGASLIRYV PEKGVLVVI SRNESSRRC 
      ARLVQDMHF RSLSQKVLL LKRTEEAAR QLESTKLAT 
      IGGMLFYFV RERKFVHVK SSRGVFGVF CSCVDDAIF 
      FCRFSDEFN VREDLMGLA IGAHGANIQ QARKVDGIT 
      NIELEENSC TFKIYGETD EAVKKARSM LEYSEESLQ 
      VPRALVGKV IGKNGRIIQ EIVDKSGVV RVKIEGDNE 
      PQPTIPREE GQVPFVFVG TVESISNAK VLLKYHLAH 
      LKEVEQLRQ EKLEIDQQL RSIHGNALG SMQSLSMSR 
      RNDRGYNSD MDGGGRPGR GSMRGRGGR GRGGGGPGG 
      RQNDRYNSG TSTITDYVN NVDKRNNAA NKPMGNGRG 
      GGRGGGGGG HASSNGRPP RGGGGPPTN NRERPNKGR 
      LSIDSAAGG DRGERK
    CDS sequence
    • >TC003774 RA
      atgtcggtt gtcggtttt ggtttagtg aaaatcttt 
      ctaggatcg cgtcaccag acgccggaa acagcggat 
      gagcgcgtc agtgatttg cccccgcgt catttcggg 
      ggtcctcgc gggcgcggc gcccgcagg gataaccga 
      agagacgag cggcgtcgc accaccgac gacgaggaa 
      acggttttg gaccttacg gaggtatcc agtgttgat 
      cgagagtct atatcgagt gcggaagcg acttggtca 
      ggtggtagg agtcagagg aggcgtaga cgtcgaggg 
      agacagcgc agccttacg ccagttatc aacgacacc 
      acagtgaat aacggtcaa gcgggtccg gccggtgac 
      gtagtgccg aacgacagt cacatgacc gacggtgga 
      gccaatcag aagccgcga ggaggcggt gacccgaag 
      aaccgatcc aatcggccg ccgccagcg cgtcgtaat 
      aacgagccc aagccgaag gaggcgctg gtgaacggc 
      accaccgcc tag
    • >TC003774 RB
      atgtcggtt gtcggtttt ggtttagtg aaaatcttt 
      ctaggatcg cgtcaccag acgccggaa acagcggat 
      gagcgcgtc agtgatttg cccccgcgt catttcggg 
      ggtcctcgc gggcgcggc gcccgcagg gataaccga 
      agagacgag cggcgtcgc accaccgac gacgaggaa 
      acggttttg gaccttacg gaggtatcc agtgttgat 
      cgagtgaat aacggtcaa gcgggtccg gccggtgac 
      gtagtgccg aacgacagt cacatgacc gacggtgga 
      gccaatcag aagccgcga ggaggcggt gacccgaag 
      aaccgatcc aatcggccg ccgccagcg cgtcgtaat 
      aacgagccc aagccgaag gaggcgctg gtgaacggc 
      accaccgcc tag
    • >TC003774 RC
      atggaggac ctggcagtg gaagtatgc ggcgaaaat 
      ggggccctt tataagggg tacgtcgtt gatgtgttt 
      gaggattcc gttcttata cattttgag gatgagtgg 
      caaccggat tcaaagttt cccttttct caagttcgt 
      ttgccccca aaacccgac ccaaaggtc gagtttacc 
      gaaaacatg gaggttgaa gtatactcc agagctaat 
      catcaagag gcttatgga tggtggaag tctagaatt 
      aagatgatg aaaggcgac ttttacgtg ttggaatac 
      gtcggctgg gacacaacg tacacggaa atagtcagc 
      gacgatcgt cttcgtgtc aaaaactct aatccaccg 
      attgacagt tcgatgttt gtcaaattc gaaatcgaa 
      gtaccagaa gacgtccgc gagtatgct aaaatagaa 
      aatgctcac aaagagttt caaaacgcc atcggtgct 
      agtctcatt aggtacgtt ccggaaaag ggtgttctc 
      gtagtgata tcgaggaac gagtcgagt cgcagatgc 
      gcgcgtctt gtgcaagac atgcatttt cgtagttta 
      tcgcaaaaa gtgctctta ttgaaacgt acggaggag 
      gcggcgagg cagttggag agtacgaag ttggcgaca 
      atcggcggc atgttgttt tattttgta cgagagaga 
      aaatttgtg catgtcaag tcgagccga ggggtgttt 
      ggtgttttt tgttcttgt gttgatgat gcgattttt 
      ttttgcagg ttttccgat gagttcaac gttagggaa 
      gatctgatg gggctagcg attggggct cacggtgcc 
      aacatacaa caagctagg aaagtcgac ggtattacg 
      aacatagaa cttgaagaa aattcttgc acgtttaaa 
      atatatgga gagactgac gaggctgtg aagaaagcc 
      cgatcgatg ttagaatat agtgaagaa tctcttcaa 
      gttccgcgg gcgctagta ggaaaagta atcggcaaa 
      aatggcaga attattcaa gaaatcgtt gacaaaagc 
      ggtgtggtt cgggttaaa atcgaaggt gacaatgag 
      ccccaaccg acgattcct cgcgaagaa ggccaagta 
      ccgtttgtg ttcgtcggt accgtagag agcatatcc 
      aatgcgaaa gtcctcctc aaatatcac ctggcacac 
      ctcaaggaa gtggaacag cttagacag gagaaactc 
      gagatcgac cagcagcta aggagcatt cacggcaac 
      gccctcggt tcgatgcag agcctgtcg atgagtcga 
      cgaaacgac cgaggctac aattcggac atggacggg 
      ggcggccgt cctggtcgc ggttcgatg cgcggccgc 
      gggggcaga ggccgaggg ggcggcggt cccggagga 
      cgacaaaac gaccgctac aattccggt actagtacg 
      ataactgat tatgttaat aacgtggat aagcgcaac 
      aacgccgcc aacaaaccg atggggaac ggcaggggc 
      ggtggacgc ggtggcggt ggcggcggt catgccagc 
      agcaatggg cggcccccg agagggggc ggcggaccg 
      ccgacgaac aaccgcgaa cgacccaac aagggatcg 
      cgtcaccag acgccggaa acagcggat gagcgcgtc 
      agtgatttg cccccgcgt catttcggg ggtcctcgc 
      gggcgcggc gcccgcagg gataaccga agagacgag 
      cggcgtcgc accaccgac gacgaggaa acggttttg 
      gaccttacg gaggtatcc agtgttgat cgagagtct 
      atatcgagt gcggaagcg acttggtca ggtggtagg 
      agtcagagg aggcgtaga cgtcgaggg agacagcgc 
      agccttacg ccagttatc aacgacacc acagtgaat 
      aacggtcaa gcgggtccg gccggtgac gtagtgccg 
      aacgacagt cacatgacc gacggtgga gccaatcag 
      aagccgcga ggaggcggt gacccgaag aaccgatcc 
      aatcggccg ccgccagcg cgtcgtaat aacgagccc 
      aagccgaag gaggcgctg gtgaacggc accaccgcc 
      tag
    • >TC003774 RD
      atggaggac ctggcagtg gaagtatgc ggcgaaaat 
      ggggccctt tataagggg tacgtcgtt gatgtgttt 
      gaggattcc gttcttata cattttgag gatgagtgg 
      caaccggat tcaaagttt cccttttct caagttcgt 
      ttgccccca aaacccgac ccaaaggtc gagtttacc 
      gaaaacatg gaggttgaa gtatactcc agagctaat 
      catcaagag gcttatgga tggtggaag tctagaatt 
      aagatgatg aaaggcgac ttttacgtg ttggaatac 
      gtcggctgg gacacaacg tacacggaa atagtcagc 
      gacgatcgt cttcgtgtc aaaaactct aatccaccg 
      attgacagt tcgatgttt gtcaaattc gaaatcgaa 
      gtaccagaa gacgtccgc gagtatgct aaaatagaa 
      aatgctcac aaagagttt caaaacgcc atcggtgct 
      agtctcatt aggtacgtt ccggaaaag ggtgttctc 
      gtagtgata tcgaggaac gagtcgagt cgcagatgc 
      gcgcgtctt gtgcaagac atgcatttt cgtagttta 
      tcgcaaaaa gtgctctta ttgaaacgt acggaggag 
      gcggcgagg cagttggag agtacgaag ttggcgaca 
      atcggcggc atgttgttt tattttgta cgagagaga 
      aaatttgtg catgtcaag tcgagccga ggggtgttt 
      ggtgttttt tgttcttgt gttgatgat gcgattttt 
      ttttgcagg ttttccgat gagttcaac gttagggaa 
      gatctgatg gggctagcg attggggct cacggtgcc 
      aacatacaa caagctagg aaagtcgac ggtattacg 
      aacatagaa cttgaagaa aattcttgc acgtttaaa 
      atatatgga gagactgac gaggctgtg aagaaagcc 
      cgatcgatg ttagaatat agtgaagaa tctcttcaa 
      gttccgcgg gcgctagta ggaaaagta atcggcaaa 
      aatggcaga attattcaa gaaatcgtt gacaaaagc 
      ggtgtggtt cgggttaaa atcgaaggt gacaatgag 
      ccccaaccg acgattcct cgcgaagaa ggccaagta 
      ccgtttgtg ttcgtcggt accgtagag agcatatcc 
      aatgcgaaa gtcctcctc aaatatcac ctggcacac 
      ctcaaggaa gtggaacag cttagacag gagaaactc 
      gagatcgac cagcagcta aggagcatt cacggcaac 
      gccctcggt tcgatgcag agcctgtcg atgagtcga 
      cgaaacgac cgaggctac aattcggac atggacggg 
      ggcggccgt cctggtcgc ggttcgatg cgcggccgc 
      gggggcaga ggccgaggg ggcggcggt cccggagga 
      cgacaaaac gaccgctac aattccggt actagtacg 
      ataactgat tatgttaat aacgtggat aagcgcaac 
      aacgccgcc aacaaaccg atggggaac ggcaggggc 
      ggtggacgc ggtggcggt ggcggcggt catgccagc 
      agcaatggg cggcccccg agagggggc ggcggaccg 
      ccgacgaac aaccgcgaa cgacccaac aagggtagg 
      ttgtccatc gactcggcc gccgggggc gacaggggg 
      gagaggaag tga
    mRNA sequence
    • >TC003774 RA
      ctcgtggtt ttactaact aatgcatgc gcatgcaaa 
      aagcttgaa gggtgtttg agtctgtgc tcactgatt 
      cgtttttct cccccttgg cgtcgtagt tggtgattt 
      gtgttagtg gcgctgttt atctacgat acgaacagt 
      taatgcaat ttaattaat tactcgttt cgtcccaat 
      tttaattac gtatagatt tctactcta ttacttatg 
      tttctacga tttggtaat caagtatca ggaagtaag 
      gttgcatga aggtttttt tgaagaaac tatttgtat 
      ttaattaaa tcactggga acgagcgat gaatgtcgg 
      ttgtcggtt ttggtttag tgaaaatct ttctaggat 
      cgcgtcacc agacgccgg aaacagcgg atgagcgcg 
      tcagtgatt tgcccccgc gtcatttcg ggggtcctc 
      gcgggcgcg gcgcccgca gggataacc gaagagacg 
      agcggcgtc gcaccaccg acgacgagg aaacggttt 
      tggacctta cggaggtat ccagtgttg atcgagagt 
      ctatatcga gtgcggaag cgacttggt caggtggta 
      ggagtcaga ggaggcgta gacgtcgag ggagacagc 
      gcagcctta cgccagtta tcaacgaca ccacagtga 
      ataacggtc aagcgggtc cggccggtg acgtagtgc 
      cgaacgaca gtcacatga ccgacggtg gagccaatc 
      agaagccgc gaggaggcg gtgacccga agaaccgat 
      ccaatcggc cgccgccag cgcgtcgta ataacgagc 
      ccaagccga aggaggcgc tggtgaacg gcaccaccg 
      cctagagca gagagaaac actgtgcgc atcattaaa 
      ccgcaacca gagagacgt tttagtttg gattttttt 
      ccttttgat tttcttttg agaaaaaat tgatattgt 
      tgatgttgt gttgcatat cttttcccg gtgtagcaa 
      aaatgttaa agtgggcgg tcgtctgcc ccatactgg 
      ccgatacca gagtgactg tttggttct gtgaacctc 
      ctcaacatc ctcaacgtt aagactgat gtcttaagt 
      gtttaagtg gtcgcagcg ggtgaagag cttagtgcg 
      attatggac agagttgat ttatactta taacctcca 
      agaattttg tgactactt ttctaatct ttcatacat 
      ttcatgttt agcgcacat aaataatgc tcgaaatta 
      taaaacttt agtcattat tctctttga aacgtgttg 
      agaatcttc actttaagt ttcagtata tttgtattt 
      atgtgttat ttattattt tttattttt tgtttatag 
      tttgtaaga taaagtgac catttgtac cttctacaa 
      atagtttac agccatttt ttcttatta tttttcctt 
      tattactgt aatgataca ttcagatgt aatgtaaat 
      aaagcctcc taaattaaa actagctaa tacagtatt 
      tggttaggt gctctgttt atgatttgt tacaatggt 
      ttatctgct gtgtattgg tataacaca tttttgcat 
      tgaataaaa cattcaata aaag
    • >TC003774 RB
      ctcgtggtt ttactaact aatgcatgc gcatgcaaa 
      aagcttgaa gggtgtttg agtctgtgc tcactgatt 
      cgtttttct cccccttgg cgtcgtagt tggtgattt 
      gtgttagtg gcgctgttt atctacgat acgaacagt 
      taatgcaat ttaattaat tactcgttt cgtcccaat 
      tttaattac gtatagatt tctactcta ttacttatg 
      tttctacga tttggtaat caagtatca ggaagtaag 
      gttgcatga aggtttttt tgaagaaac tatttgtat 
      ttaattaaa tcactggga acgagcgat gaatgtcgg 
      ttgtcggtt ttggtttag tgaaaatct ttctaggat 
      cgcgtcacc agacgccgg aaacagcgg atgagcgcg 
      tcagtgatt tgcccccgc gtcatttcg ggggtcctc 
      gcgggcgcg gcgcccgca gggataacc gaagagacg 
      agcggcgtc gcaccaccg acgacgagg aaacggttt 
      tggacctta cggaggtat ccagtgttg atcgagtga 
      ataacggtc aagcgggtc cggccggtg acgtagtgc 
      cgaacgaca gtcacatga ccgacggtg gagccaatc 
      agaagccgc gaggaggcg gtgacccga agaaccgat 
      ccaatcggc cgccgccag cgcgtcgta ataacgagc 
      ccaagccga aggaggcgc tggtgaacg gcaccaccg 
      cctagagca gagagaaac actgtgcgc atcattaaa 
      ccgcaacca gagagacgt tttagtttg gattttttt 
      ccttttgat tttcttttg agaaaaaat tgatattgt 
      tgatgttgt gttgcatat cttttcccg gtgtagcaa 
      aaatgttaa agtgggcgg tcgtctgcc ccatactgg 
      ccgatacca gagtgactg tttggttct gtgaacctc 
      ctcaacatc ctcaacgtt aagactgat gtcttaagt 
      gtttaagtg gtcgcagcg ggtgaagag cttagtgcg 
      attatggac agagttgat ttatactta taacctcca 
      agaattttg tgactactt ttctaatct ttcatacat 
      ttcatgttt agcgcacat aaataatgc tcgaaatta 
      taaaacttt agtcattat tctctttga aacgtgttg 
      agaatcttc actttaagt ttcagtata tttgtattt 
      atgtgttat ttattattt tttattttt tgtttatag 
      tttgtaaga taaagtgac catttgtac cttctacaa 
      atagtttac agccatttt ttcttatta tttttcctt 
      tattactgt aatgataca ttcagatgt aatgtaaat 
      aaagcctcc taaattaaa actagctaa tacagtatt 
      tggttaggt gctctgttt atgatttgt tacaatggt 
      ttatctgct gtgtattgg tataacaca tttttgcat 
      tgaataaaa cattcaata aaag
    • >TC003774 RC
      agtggcagc agtgcaact ccctctcgg ctcaaggct 
      gtttcctca cccaagaac aaaaccaac atggaggac 
      ctggcagtg gaagtatgc ggcgaaaat ggggccctt 
      tataagggg tacgtcgtt gatgtgttt gaggattcc 
      gttcttata cattttgag gatgagtgg caaccggat 
      tcaaagttt cccttttct caagttcgt ttgccccca 
      aaacccgac ccaaaggtc gagtttacc gaaaacatg 
      gaggttgaa gtatactcc agagctaat catcaagag 
      gcttatgga tggtggaag tctagaatt aagatgatg 
      aaaggcgac ttttacgtg ttggaatac gtcggctgg 
      gacacaacg tacacggaa atagtcagc gacgatcgt 
      cttcgtgtc aaaaactct aatccaccg attgacagt 
      tcgatgttt gtcaaattc gaaatcgaa gtaccagaa 
      gacgtccgc gagtatgct aaaatagaa aatgctcac 
      aaagagttt caaaacgcc atcggtgct agtctcatt 
      aggtacgtt ccggaaaag ggtgttctc gtagtgata 
      tcgaggaac gagtcgagt cgcagatgc gcgcgtctt 
      gtgcaagac atgcatttt cgtagttta tcgcaaaaa 
      gtgctctta ttgaaacgt acggaggag gcggcgagg 
      cagttggag agtacgaag ttggcgaca atcggcggc 
      atgttgttt tattttgta cgagagaga aaatttgtg 
      catgtcaag tcgagccga ggggtgttt ggtgttttt 
      tgttcttgt gttgatgat gcgattttt ttttgcagg 
      ttttccgat gagttcaac gttagggaa gatctgatg 
      gggctagcg attggggct cacggtgcc aacatacaa 
      caagctagg aaagtcgac ggtattacg aacatagaa 
      cttgaagaa aattcttgc acgtttaaa atatatgga 
      gagactgac gaggctgtg aagaaagcc cgatcgatg 
      ttagaatat agtgaagaa tctcttcaa gttccgcgg 
      gcgctagta ggaaaagta atcggcaaa aatggcaga 
      attattcaa gaaatcgtt gacaaaagc ggtgtggtt 
      cgggttaaa atcgaaggt gacaatgag ccccaaccg 
      acgattcct cgcgaagaa ggccaagta ccgtttgtg 
      ttcgtcggt accgtagag agcatatcc aatgcgaaa 
      gtcctcctc aaatatcac ctggcacac ctcaaggaa 
      gtggaacag cttagacag gagaaactc gagatcgac 
      cagcagcta aggagcatt cacggcaac gccctcggt 
      tcgatgcag agcctgtcg atgagtcga cgaaacgac 
      cgaggctac aattcggac atggacggg ggcggccgt 
      cctggtcgc ggttcgatg cgcggccgc gggggcaga 
      ggccgaggg ggcggcggt cccggagga cgacaaaac 
      gaccgctac aattccggt actagtacg ataactgat 
      tatgttaat aacgtggat aagcgcaac aacgccgcc 
      aacaaaccg atggggaac ggcaggggc ggtggacgc 
      ggtggcggt ggcggcggt catgccagc agcaatggg 
      cggcccccg agagggggc ggcggaccg ccgacgaac 
      aaccgcgaa cgacccaac aagggatcg cgtcaccag 
      acgccggaa acagcggat gagcgcgtc agtgatttg 
      cccccgcgt catttcggg ggtcctcgc gggcgcggc 
      gcccgcagg gataaccga agagacgag cggcgtcgc 
      accaccgac gacgaggaa acggttttg gaccttacg 
      gaggtatcc agtgttgat cgagagtct atatcgagt 
      gcggaagcg acttggtca ggtggtagg agtcagagg 
      aggcgtaga cgtcgaggg agacagcgc agccttacg 
      ccagttatc aacgacacc acagtgaat aacggtcaa 
      gcgggtccg gccggtgac gtagtgccg aacgacagt 
      cacatgacc gacggtgga gccaatcag aagccgcga 
      ggaggcggt gacccgaag aaccgatcc aatcggccg 
      ccgccagcg cgtcgtaat aacgagccc aagccgaag 
      gaggcgctg gtgaacggc accaccgcc tagagcaga 
      gagaaacac tgtgcgcat cattaaacc gcaaccaga 
      gagacgttt tagtttgga tttttttcc ttttgattt 
      tcttttgag aaaaaattg atattgttg atgttgtgt 
      tgcatatct tttcccggt gtagcaaaa atgttaaag 
      tgggcggtc gtctgcccc atactggcc gataccaga 
      gtgactgtt tggttctgt gaacctcct caacatcct 
      caacgttaa gactgatgt cttaagtgt ttaagtggt 
      cgcagcggg tgaagagct tagtgcgat tatggacag 
      agttgattt atacttata acctccaag aattttgtg 
      actactttt ctaatcttt catacattt catgtttag 
      cgcacataa ataatgctc gaaattata aaactttag 
      tcattattc tctttgaaa cgtgttgag aatcttcac 
      tttaagttt cagtatatt tgtatttat gtgttattt 
      attattttt tattttttg tttatagtt tgtaagata 
      aagtgacca tttgtacct tctacaaat agtttacag 
      ccatttttt cttattatt tttccttta ttactgtaa 
      tgatacatt cagatgtaa tgtaaataa agcctccta 
      aattaaaac tagctaata cagtatttg gttaggtgc 
      tctgtttat gatttgtta caatggttt atctgctgt 
      gtattggta taacacatt tttgcattg aataaaaca 
      ttcaataaa ag
    • >TC003774 RD
      agtggcagc agtgcaact ccctctcgg ctcaaggct 
      gtttcctca cccaagaac aaaaccaac atggaggac 
      ctggcagtg gaagtatgc ggcgaaaat ggggccctt 
      tataagggg tacgtcgtt gatgtgttt gaggattcc 
      gttcttata cattttgag gatgagtgg caaccggat 
      tcaaagttt cccttttct caagttcgt ttgccccca 
      aaacccgac ccaaaggtc gagtttacc gaaaacatg 
      gaggttgaa gtatactcc agagctaat catcaagag 
      gcttatgga tggtggaag tctagaatt aagatgatg 
      aaaggcgac ttttacgtg ttggaatac gtcggctgg 
      gacacaacg tacacggaa atagtcagc gacgatcgt 
      cttcgtgtc aaaaactct aatccaccg attgacagt 
      tcgatgttt gtcaaattc gaaatcgaa gtaccagaa 
      gacgtccgc gagtatgct aaaatagaa aatgctcac 
      aaagagttt caaaacgcc atcggtgct agtctcatt 
      aggtacgtt ccggaaaag ggtgttctc gtagtgata 
      tcgaggaac gagtcgagt cgcagatgc gcgcgtctt 
      gtgcaagac atgcatttt cgtagttta tcgcaaaaa 
      gtgctctta ttgaaacgt acggaggag gcggcgagg 
      cagttggag agtacgaag ttggcgaca atcggcggc 
      atgttgttt tattttgta cgagagaga aaatttgtg 
      catgtcaag tcgagccga ggggtgttt ggtgttttt 
      tgttcttgt gttgatgat gcgattttt ttttgcagg 
      ttttccgat gagttcaac gttagggaa gatctgatg 
      gggctagcg attggggct cacggtgcc aacatacaa 
      caagctagg aaagtcgac ggtattacg aacatagaa 
      cttgaagaa aattcttgc acgtttaaa atatatgga 
      gagactgac gaggctgtg aagaaagcc cgatcgatg 
      ttagaatat agtgaagaa tctcttcaa gttccgcgg 
      gcgctagta ggaaaagta atcggcaaa aatggcaga 
      attattcaa gaaatcgtt gacaaaagc ggtgtggtt 
      cgggttaaa atcgaaggt gacaatgag ccccaaccg 
      acgattcct cgcgaagaa ggccaagta ccgtttgtg 
      ttcgtcggt accgtagag agcatatcc aatgcgaaa 
      gtcctcctc aaatatcac ctggcacac ctcaaggaa 
      gtggaacag cttagacag gagaaactc gagatcgac 
      cagcagcta aggagcatt cacggcaac gccctcggt 
      tcgatgcag agcctgtcg atgagtcga cgaaacgac 
      cgaggctac aattcggac atggacggg ggcggccgt 
      cctggtcgc ggttcgatg cgcggccgc gggggcaga 
      ggccgaggg ggcggcggt cccggagga cgacaaaac 
      gaccgctac aattccggt actagtacg ataactgat 
      tatgttaat aacgtggat aagcgcaac aacgccgcc 
      aacaaaccg atggggaac ggcaggggc ggtggacgc 
      ggtggcggt ggcggcggt catgccagc agcaatggg 
      cggcccccg agagggggc ggcggaccg ccgacgaac 
      aaccgcgaa cgacccaac aagggtagg ttgtccatc 
      gactcggcc gccgggggc gacaggggg gagaggaag 
      tgatggggg ctgatgacg acacgcccc gtcattaac 
      accggaatg gctttatgc agcgcgtcc ggttttatt 
      gttgttgtt ttcattttc taacgttgg taatgagga 
      tttttgaga actgcgacg gttgcataa agccctaaa 
      atcatttca aaatcagta aatatagac tgacccggc 
      tgacgacaa tttttaaac gatttttaa aacgacgat 
      atgacacga aatgacgtt taccaactt aaaaatttg 
      gaaattttt gcttgatta acacttttt taccttcaa 
      ttctcttgc taataacac actaaattg taacaagtg 
      aatgtacat aatttttgt ttggcttca aatttttgt 
      cccttaatt acgaattaa tttcttccc tgtccctcc 
      ccagttttt ttctcttct ggatttatt ttttttagt 
      ttgacttac tttaatgtt tacgctgta ttatagtac 
      agtaatgta aatgtaatc ataaatatt ggatttgtt 
      gctaacgag cattgtcgc taatgaaat gtgacttaa 
      tttcaaggc tttgttgtg acggtgggt gcagaaatg 
      agagtcctt gtgattttt gttcgtttt cccttagtt 
      cggttgata tctgcacct tcccgaata cgtattgtc 
      aataagata attagattg gttacttta aataacaga 
      caaaagtcg tgatttgtt tgttgtttg agctgacct 
      agaagttag taaaccccc atgaggctt ctaaatatc 
      tcgacaaaa aaaataacc ttgctggaa gggtacgca 
      aaatcgatt tagccatgt gtagactac gaaaagtga 
      gtgaaaaaa cgcgacgaa acgcggttt gtgctcact 
      gtgagtttg ctaacacta cttaaggaa tttaaatac 
      caaaaaatg ggcaatagt gactgccat ggcgaaaga 
      ctggctcac taaaaactg tgtcacgta tctaattaa 
      ttgtgaaga cttttttag aaatataac ttgatttaa 
      aaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY