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Gene information
General information
TC003773 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 17808282..17814549
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003773
    iBeetle screen iB_03617

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003773 RA
      MLRPILLLL LIASCRPYR KRPEDKTKC 
      PSVKAIRNF NLEQMMGRW YVIQYYASS 
      EEALSYRCM RAEFSMSPF LADVTMNFT 
      YSFTDDPLN ELLVGNITW TVPNPGVPS 
      HWTHSEDTY EGVYNTFVL DSDYTSWAL 
      LLHCAEKSK VPRYLSSFI MSREPVLGV 
      NVISYLRDK LPRYDIDLS YMFEMSQND 
      CNSTVIASD LPPALVAQR LPPSLRRHP 
      MKHHH
    CDS sequence
    • >TC003773 RA
      atgctgcgt cccatcctg ctcctcctg ctcatcgcc 
      tcctgcagg ccctaccgc aaacggccc gaggacaag 
      accaaatgt cccagcgtc aaagccatc aggaacttc 
      aatttggaa cagatgatg gggcgatgg tacgtcatc 
      cagtactat gccagctcg gaagaggcg ctgtcgtac 
      aggtgcatg agggcagag ttcagcatg tcgcccttt 
      ttggccgat gtcaccatg aattttact tacagcttc 
      acggatgat ccgcttaat gagctgctg gttggaaac 
      atcacgtgg acggtaccg aatccggga gtgccctcg 
      cattggaca cattcggag gacacgtat gagggtgtg 
      tacaatacg ttcgttttg gactcggat tacacctcc 
      tgggccttg ctcctccac tgtgcggag aaatctaaa 
      gttcctcgc tacctatcc agcttcata atgagtcga 
      gaaccagtt ctcggcgtc aacgttatt tcttatctc 
      cgagataaa ctaccaaga tacgacata gacctatct 
      tacatgttc gaaatgtcc caaaatgac tgtaattcg 
      acggtaatc gcctcggac ctacctcca gcacttgtg 
      gcccagcga ctaccaccg tcgctacga cgacatccc 
      atgaaacat caccattag
    mRNA sequence
    • >TC003773 RA
      ctcttcaaa cggaagcaa agaccacaa caaatgacc 
      aaactctag tccgccaca atgctgcgt cccatcctg 
      ctcctcctg ctcatcgcc tcctgcagg ccctaccgc 
      aaacggccc gaggacaag accaaatgt cccagcgtc 
      aaagccatc aggaacttc aatttggaa cagatgatg 
      gggcgatgg tacgtcatc cagtactat gccagctcg 
      gaagaggcg ctgtcgtac aggtgcatg agggcagag 
      ttcagcatg tcgcccttt ttggccgat gtcaccatg 
      aattttact tacagcttc acggatgat ccgcttaat 
      gagctgctg gttggaaac atcacgtgg acggtaccg 
      aatccggga gtgccctcg cattggaca cattcggag 
      gacacgtat gagggtgtg tacaatacg ttcgttttg 
      gactcggat tacacctcc tgggccttg ctcctccac 
      tgtgcggag aaatctaaa gttcctcgc tacctatcc 
      agcttcata atgagtcga gaaccagtt ctcggcgtc 
      aacgttatt tcttatctc cgagataaa ctaccaaga 
      tacgacata gacctatct tacatgttc gaaatgtcc 
      caaaatgac tgtaattcg acggtaatc gcctcggac 
      ctacctcca gcacttgtg gcccagcga ctaccaccg 
      tcgctacga cgacatccc atgaaacat caccattag 
      gcaaccacg acaccgctt aacggaact ttaccataa 
      aaataaaca agtccagtt ctcaattag tgtgcatta 
      gaactggat gaaataatg cacatgtag aaaaacacc 
      cgtatgtaa cacaactgc tcacagtaa taaaatttt 
      tggctaccc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY