Status
Please wait ...
Gene information
General information
TC003770 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 17855173..17880167
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003770
    iBeetle screen iB_03616

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    remarks: later than schedule, hours 48 adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    remarks: later than schedule, hours 48 remarks: later than schedule, hours 48
    Pupal morphology
    remarks: later than schedule, hours 48
    Adult morphology
    remarks: later than schedule, hours 48 remarks: later than schedule, hours 48
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 remarks: later than schedule, hours 48 (egglay)
    remarks: later than schedule, hours 48
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    remarks: later than schedule, hours 48
    remarks - cuticles: later than schedule, hours 48
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Pupal morphology
    antenna orientation bent - Number of animals showing the phenotype: 8

    elytra size shortened - Number of animals showing the phenotype: 8

    Adult morphology
    antenna orientation bent - Number of animals showing the phenotype: 8


    elytra dorsal orientation not closed - Number of animals showing the phenotype: 8


    elytra size shortened - Number of animals showing the phenotype: 8


    Fertility
    egg production: number no/almost no eggs -
    Comment: No phenotype found!
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003770 RA
      MLSPPRHLR RLLLLPVIW CFSFILLLI NVVDGAGDL 
      WPLDRPDGM PSIQSLEVM CGKDHMDVH LTFSQPFEG 
      IVSSKGQHG DPRCVYVPP STGQTFFSF RIAYARCGT 
      KPDLHGQFY ENTVVVQYD KDLLEVWDE AKRLRCEWF 
      NDYEKTASK PPMVIADLD VVQLDFRGD NVDCWMEIQ 
      HGKGPWAPP VSGIVPLGS TLTLVVAIN DYRGEFDMR 
      VKSCVASDG GGHVIQLSD EFGCVLRPK MISRFLKAR 
      GADERASVI TYAFFHAFK FPDALSVHI KCKVEICRH 
      GCLDHCQIQ PEKGDVDSE TYLGPLDRK DDKLESGEK 
      TGDPDVLYE DVISEGGVD GVSSVKMSL NAQLGDSKP 
      MSNKPAGLD DDKELDITA VPHKDLFLL HDQFPHGPR 
      TFEDRPVAA PRAFDAAST PPLDRLKRR RRSVTVSDR 
      KARSADVGV SGLYEVISE ADLAFSPDT RAEAVTVFQ 
      GRIREEVVY GICMPVPGF SVLFVLVAV SAVVSALVA 
      GSLLYRYQL QKEQLAEQA RNGPIPMGL FSGWVGWRL 
      LRMRAHPVA QNGTDKRVR QVNGKTDET SRCE
    CDS sequence
    • >TC003770 RA
      atgctgagc ccacctcgc caccttagg cgactttta 
      ctcctgcct gtgatttgg tgtttttct ttcatttta 
      ttgctgatc aatgtcgtg gacggagcg ggcgatctt 
      tggcctctg gaccgtccc gacggcatg ccttccatc 
      cagtcgctc gaggtcatg tgcggcaag gaccacatg 
      gacgtgcac ttgaccttc tcgcagccc ttcgagggc 
      atcgtctcc tccaagggg cagcacggc gacccgcgc 
      tgcgtctac gtgccgccc tccacgggc cagaccttc 
      ttctcgttt cggatcgcg tacgcccga tgcgggacc 
      aaacccgat ttacacgga cagttttac gaaaacacg 
      gtggtagtg caatatgat aaagacttg ttagaagtg 
      tgggacgag gcgaaaaga ctgagatgt gagtggttc 
      aatgactac gagaaaact gcctccaaa ccgcccatg 
      gtcatcgcc gacttggac gtcgtccag ttggacttc 
      cgaggtgac aacgtcgac tgctggatg gagatccag 
      cacgggaaa ggaccatgg gcaccgcca gtcagcgga 
      atagtacca ttgggatca accctgacc ctcgtagtg 
      gccatcaac gattacaga ggagagttc gacatgcgg 
      gtcaaatcg tgcgtcgcg tctgatgga ggaggccac 
      gtcatccag ttatcggac gaattcggg tgcgtcctc 
      cgcccgaaa atgatcagt cgcttcctc aaggcccgc 
      ggcgcggac gagcgggcg tctgtcata acttacgcc 
      ttcttccac gctttcaaa ttcccggac gctctgagc 
      gtccacatc aagtgcaag gtggagata tgccgccat 
      gggtgtctg gaccactgc cagatccag ccggagaag 
      ggcgacgtg gactcggag acgtacttg ggcccgctg 
      gaccgcaag gacgacaag ctggagtcc ggggagaag 
      accggcgat ccggacgtg ttgtacgaa gacgtgata 
      agtgaggga ggtgtcgat ggggtatct tcagtgaag 
      atgtcgctg aacgctcag ctcggcgac agcaagccg 
      atgtcgaac aagccggcc ggacttgac gatgataaa 
      gaactggat ataacggca gtaccgcat aaggacctc 
      ttcctcctg cacgaccag ttcccgcac gggccgcgc 
      acgttcgag gaccgtccc gtggccgcc cctcgcgcc 
      ttcgacgcc gcctccacg ccgcccctc gaccgcctc 
      aagcgccgc cgccgctcg gtcaccgtc tccgaccgc 
      aaggcccgc agcgccgac gtgggcgtc agcggcctc 
      tacgaagtc atctccgag gcggacctg gccttcagc 
      cccgacacc cgcgccgag gccgtcacc gtcttccag 
      ggccgcatc cgcgaggag gtcgtctac ggcatctgc 
      atgccggtg cccggcttc agcgtgctc ttcgtcctg 
      gtggccgtc tccgccgtg gtgtcggcg ctcgtcgcc 
      ggctcgctg ctctacagg taccaactc cagaaggag 
      cagctggcc gagcaggcc cgcaacggg cccatcccc 
      atgggcctg ttctcgggc tgggtgggc tggaggctg 
      ctgcgcatg cgcgcgcac cctgtcgcc cagaacggc 
      acggacaag agggtccgg caggttaac ggtaagact 
      gacgaaacg tcgcggtgt gaatag
    mRNA sequence
    • >TC003770 RA
      ttctttggc aaaagctcc tctgcaaga cacgcgcgc 
      cggccatga tacgcgtga tggtgaccg atggattaa 
      aatttacaa gacggtgat gtggtggca acactctgc 
      gacagtgct acgagactg atgacatcg ccaaaaggg 
      ccacggaaa aatgctgag cccacctcg ccaccttag 
      gcgactttt actcctgcc tgtgatttg gtgtttttc 
      tttcatttt attgctgat caatgtcgt ggacggagc 
      gggcgatct ttggcctct ggaccgtcc cgacggcat 
      gccttccat ccagtcgct cgaggtcat gtgcggcaa 
      ggaccacat ggacgtgca cttgacctt ctcgcagcc 
      cttcgaggg catcgtctc ctccaaggg gcagcacgg 
      cgacccgcg ctgcgtcta cgtgccgcc ctccacggg 
      ccagacctt cttctcgtt tcggatcgc gtacgcccg 
      atgcgggac caaacccga tttacacgg acagtttta 
      cgaaaacac ggtggtagt gcaatatga taaagactt 
      gttagaagt gtgggacga ggcgaaaag actgagatg 
      tgagtggtt caatgacta cgagaaaac tgcctccaa 
      accgcccat ggtcatcgc cgacttgga cgtcgtcca 
      gttggactt ccgaggtga caacgtcga ctgctggat 
      ggagatcca gcacgggaa aggaccatg ggcaccgcc 
      agtcagcgg aatagtacc attgggatc aaccctgac 
      cctcgtagt ggccatcaa cgattacag aggagagtt 
      cgacatgcg ggtcaaatc gtgcgtcgc gtctgatgg 
      aggaggcca cgtcatcca gttatcgga cgaattcgg 
      gtgcgtcct ccgcccgaa aatgatcag tcgcttcct 
      caaggcccg cggcgcgga cgagcgggc gtctgtcat 
      aacttacgc cttcttcca cgctttcaa attcccgga 
      cgctctgag cgtccacat caagtgcaa ggtggagat 
      atgccgcca tgggtgtct ggaccactg ccagatcca 
      gccggagaa gggcgacgt ggactcgga gacgtactt 
      gggcccgct ggaccgcaa ggacgacaa gctggagtc 
      cggggagaa gaccggcga tccggacgt gttgtacga 
      agacgtgat aagtgaggg aggtgtcga tggggtatc 
      ttcagtgaa gatgtcgct gaacgctca gctcggcga 
      cagcaagcc gatgtcgaa caagccggc cggacttga 
      cgatgataa agaactgga tataacggc agtaccgca 
      taaggacct cttcctcct gcacgacca gttcccgca 
      cgggccgcg cacgttcga ggaccgtcc cgtggccgc 
      ccctcgcgc cttcgacgc cgcctccac gccgcccct 
      cgaccgcct caagcgccg ccgccgctc ggtcaccgt 
      ctccgaccg caaggcccg cagcgccga cgtgggcgt 
      cagcggcct ctacgaagt catctccga ggcggacct 
      ggccttcag ccccgacac ccgcgccga ggccgtcac 
      cgtcttcca gggccgcat ccgcgagga ggtcgtcta 
      cggcatctg catgccggt gcccggctt cagcgtgct 
      cttcgtcct ggtggccgt ctccgccgt ggtgtcggc 
      gctcgtcgc cggctcgct gctctacag gtaccaact 
      ccagaagga gcagctggc cgagcaggc ccgcaacgg 
      gcccatccc catgggcct gttctcggg ctgggtggg 
      ctggaggct gctgcgcat gcgcgcgca ccctgtcgc 
      ccagaacgg cacggacaa gagggtccg gcaggttaa 
      cggtaagac tgacgaaac gtcgcggtg tgaatagtg 
      gcgagtgga cttcacggc cctatttgc agggttttc 
      atactccaa atagaaccg ttgcacctc ttctaaact 
      ttttttgtt tgttgtatt ttacgagat tcaatcata 
      ttgttttgt tagatgata tgaaagggc aaatttgca 
      atatgatat gtatagatg acattatat acgtaccag 
      gtgcacaca aagcaggga accaagcgt tttttttca 
      taaactaat tactttttt aagtacatg attacataa 
      taaaataaa aaacatcag aacat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY