Status
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Gene information
General information
TC003767 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 17893929..17898664
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003767
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03615

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003767 RA
      MSDTDDTDE LLLIPPDFF TSNPALNPD NLGPYYRVV 
      DGLITQVNH LENRLSFIE SSSEFSLSL ISDSPEMYS 
      PSRLRKFGA DDGSKATSS QSTPQKPRT KFTMNSLPN 
      SPDIEHLKK PTLNKGKKS EYNPMLGEI DNFLSNVKF 
      IKRLNTVRN LESDFNSMD GVAKNLDMG QVNEMLKEI 
      ELEEAKEKP CVKDKLWQT GDNMGAVPD YGFGMRNNM 
      YPEAKKTES DSSDSTQAT VFEKFNDCN LGLVSLSKL 
      WNSNDTRGL PSKFQQKLQ EERLRRQHC EQAIQELQN 
      RVLELQERV AVAIQVDEA KDKAILRFR ESWEKAGIR 
      LQDIEKQKE KLEEEVDKL RRKLNEDSD ETTQKIAHY 
      EKEASLALN IAHSSQDKL VVLEKRCQQ LENELRTAE 
      INSEEFQQK YLQELEKSK QYPEILAAK ELELKNCKL 
      VLSDARKEI TQCQKAVET CQSELVSIK NENESLLAK 
      LDTEKENNK RLTEQKNKL LDEIAAHKS ITRGLQDEL 
      SKSREQLET NKLELKSFY QGQVELVVQ SKLKEFQKQ 
      LDEAEKALK EELSRKELS IAKTAASHI QQISEKHSL 
      EVKLLEEKH QEESRLYHL QITQLRHEL DEANHKIKR 
      QHEKQAHIE RQLHKFMES LRQDHVTTL DEMNSFRSK 
      SLEEILSFN MAEEEDKMG ETPVSSRPE KGTRRKDGL 
      QETIQMLLD RKGQGDEGC SVTKEKMRD MKGKPPWK
    CDS sequence
    • >TC003767 RA
      atgagcgac acagatgac acggatgaa ctcctctta 
      atacctccc gattttttt acctcaaac cctgccctt 
      aaccctgat aacctcggc ccttattac agagttgtg 
      gacggtttg attacccaa gtgaaccac ttggaaaat 
      cggctcagt tttattgaa agttcgtcc gagtttagt 
      cttagtcta ataagtgat tcaccggaa atgtattca 
      ccctcgcga ctacgtaaa tttggggct gacgatggg 
      agtaaagca acgtcgagt cagagcacc ccccagaag 
      ccacggaca aaatttacg atgaattcc ctgccaaat 
      agccccgat attgagcat ttgaaaaag ccaaccctt 
      aacaaaggg aaaaagtcg gaatataac ccaatgttg 
      ggcgaaatc gataatttc ttatcaaac gtcaagttt 
      atcaagcgt ttaaatacg gtgaggaac ctcgaaagt 
      gactttaat tccatggac ggggttgca aaaaaccta 
      gatatgggg caagtcaac gaaatgcta aaagaaatc 
      gagttagaa gaagcaaaa gagaaacct tgtgttaag 
      gacaagttg tggcaaact ggagacaac atgggggct 
      gtccctgac tacgggttc ggaatgagg aacaatatg 
      tacccagag gcaaaaaaa accgagtct gatagttca 
      gactccaca caagcaaca gtttttgaa aaattcaac 
      gattgtaat ttagggtta gtcagttta tccaaattg 
      tggaattcg aacgacaca aggggtctt ccgagcaaa 
      tttcagcag aaattgcaa gaagagagg ctgcgaaga 
      cagcattgc gaacaagca attcaagaa ttacaaaat 
      cgcgtatta gaattgcaa gaacgcgta gctgtcgcg 
      atccaagtc gacgaagct aaagataaa gcgatttta 
      cgttttcgc gaatcttgg gaaaaagcc ggaattaga 
      ttacaagac atagaaaaa cagaaagag aaattagag 
      gaggaggtt gataaattg agaaggaaa ttaaatgag 
      gacagtgat gaaacgact cagaaaatt gcacattat 
      gaaaaagag gcctcgctc gcacttaac atagcccac 
      agtagtcaa gataaatta gtagtttta gaaaaacgg 
      tgtcaacaa ttggaaaat gagctgcga acggctgaa 
      ataaactcg gaagagttt caacagaag tatttacaa 
      gaattggaa aaaagcaaa caatatccc gagatttta 
      gcagcgaaa gaacttgag cttaaaaac tgtaaattg 
      gtgttatcg gatgctagg aaagaaatc actcagtgc 
      caaaaagcg gtcgaaacg tgtcagtct gaacttgta 
      tcaattaaa aacgaaaat gaatctcta cttgcaaaa 
      ctagacaca gaaaaagaa aataataaa cgactcact 
      gagcaaaaa aacaaatta ttggacgaa atcgccgct 
      cacaaaagt atcacgcgc ggcctccag gacgagttg 
      agcaaaagt cgggaacag ctcgaaact aacaaactc 
      gaacttaaa agtttctac caaggtcag gtggagctc 
      gtggtccaa agcaaactc aaagagttc caaaaacag 
      ctcgacgag gccgaaaag gccctcaaa gaggagctt 
      tcgcgcaag gaattgtca atcgccaaa acagcagcc 
      tcccacatc cagcaaatt tcggaaaag cacagcttg 
      gaggttaag ctccttgag gagaaacac caagaagaa 
      tcccggctg taccacctc caaatcacg caactgcgg 
      cacgaattg gacgaagcc aaccacaaa attaagcga 
      caacacgag aaacaggcc catattgag cgacaactc 
      cataagttc atggagagt ttaaggcag gaccatgtg 
      acaacgctg gatgagatg aattcgttc cggtccaag 
      tctttggag gagattttg tcgttcaat atggcggag 
      gaggaggac aagatgggg gaaacaccg gtttcgagt 
      cgaccggaa aagggcacg agacgcaaa gacgggtta 
      caggaaacg attcagatg ctgttggac agaaaagga 
      caaggcgat gagggctgc agtgtcacg aaggagaaa 
      atgcgcgat atgaagggg aaaccaccc tggaagtga
    mRNA sequence
    • >TC003767 RA
      gtcggtcgc aaagtatgt attttgcgt ttgttgaca 
      aacaagtca acttgtttg tttgaatca aagtttgtg 
      ccgttcctc ttgttattt cgcaaaacc cgacacaag 
      tgtccctcc ttacgatgc ccttccttg cttagaggt 
      ccccctgag tggttccct gccccccaa ttaggttat 
      gtttcacct tttgctagc ccccgttgt aagtaatga 
      gcgacacag atgacacgg atgaactcc tcttaatac 
      ctcccgatt tttttacct caaaccctg cccttaacc 
      ctgataacc tcggccctt attacagag ttgtggacg 
      gtttgatta cccaagtga accacttgg aaaatcggc 
      tcagtttta ttgaaagtt cgtccgagt ttagtctta 
      gtctaataa gtgattcac cggaaatgt attcaccct 
      cgcgactac gtaaatttg gggctgacg atgggagta 
      aagcaacgt cgagtcaga gcacccccc agaagccac 
      ggacaaaat ttacgatga attccctgc caaatagcc 
      ccgatattg agcatttga aaaagccaa cccttaaca 
      aagggaaaa agtcggaat ataacccaa tgttgggcg 
      aaatcgata atttcttat caaacgtca agtttatca 
      agcgtttaa atacggtga ggaacctcg aaagtgact 
      ttaattcca tggacgggg ttgcaaaaa acctagata 
      tggggcaag tcaacgaaa tgctaaaag aaatcgagt 
      tagaagaag caaaagaga aaccttgtg ttaaggaca 
      agttgtggc aaactggag acaacatgg gggctgtcc 
      ctgactacg ggttcggaa tgaggaaca atatgtacc 
      cagaggcaa aaaaaaccg agtctgata gttcagact 
      ccacacaag caacagttt ttgaaaaat tcaacgatt 
      gtaatttag ggttagtca gtttatcca aattgtgga 
      attcgaacg acacaaggg gtcttccga gcaaatttc 
      agcagaaat tgcaagaag agaggctgc gaagacagc 
      attgcgaac aagcaattc aagaattac aaaatcgcg 
      tattagaat tgcaagaac gcgtagctg tcgcgatcc 
      aagtcgacg aagctaaag ataaagcga ttttacgtt 
      ttcgcgaat cttgggaaa aagccggaa ttagattac 
      aagacatag aaaaacaga aagagaaat tagaggagg 
      aggttgata aattgagaa ggaaattaa atgaggaca 
      gtgatgaaa cgactcaga aaattgcac attatgaaa 
      aagaggcct cgctcgcac ttaacatag cccacagta 
      gtcaagata aattagtag ttttagaaa aacggtgtc 
      aacaattgg aaaatgagc tgcgaacgg ctgaaataa 
      actcggaag agtttcaac agaagtatt tacaagaat 
      tggaaaaaa gcaaacaat atcccgaga ttttagcag 
      cgaaagaac ttgagctta aaaactgta aattggtgt 
      tatcggatg ctaggaaag aaatcactc agtgccaaa 
      aagcggtcg aaacgtgtc agtctgaac ttgtatcaa 
      ttaaaaacg aaaatgaat ctctacttg caaaactag 
      acacagaaa aagaaaata ataaacgac tcactgagc 
      aaaaaaaca aattattgg acgaaatcg ccgctcaca 
      aaagtatca cgcgcggcc tccaggacg agttgagca 
      aaagtcggg aacagctcg aaactaaca aactcgaac 
      ttaaaagtt tctaccaag gtcaggtgg agctcgtgg 
      tccaaagca aactcaaag agttccaaa aacagctcg 
      acgaggccg aaaaggccc tcaaagagg agctttcgc 
      gcaaggaat tgtcaatcg ccaaaacag cagcctccc 
      acatccagc aaatttcgg aaaagcaca gcttggagg 
      ttaagctcc ttgaggaga aacaccaag aagaatccc 
      ggctgtacc acctccaaa tcacgcaac tgcggcacg 
      aattggacg aagccaacc acaaaatta agcgacaac 
      acgagaaac aggcccata ttgagcgac aactccata 
      agttcatgg agagtttaa ggcaggacc atgtgacaa 
      cgctggatg agatgaatt cgttccggt ccaagtctt 
      tggaggaga ttttgtcgt tcaatatgg cggaggagg 
      aggacaaga tgggggaaa caccggttt cgagtcgac 
      cggaaaagg gcacgagac gcaaagacg ggttacagg 
      aaacgattc agatgctgt tggacagaa aaggacaag 
      gcgatgagg gctgcagtg tcacgaagg agaaaatgc 
      gcgatatga aggggaaac caccctgga agtgattct 
      attgatttt ttatacttt atttattta ttttttaat 
      aaattattg ttttttttt atttttaat aaagtaata 
      aatgagtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY