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Gene information
General information
TC003763 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 18020620..18062976
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003763
    iBeetle screen iB_03614

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 5 (41 dpi)
    Days post injection

    Fertility
    egg production: number no/almost no eggs -
    Comment: No phenotype found!
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003763 RA
      MSWPRRALF AMLVVAAYS RGLGEDVEA EIVEAPDKL 
PAPPPSPLD DCDPELIGF ELITGYVFS APGNVLDSI 
PGTLMLTDC LETCQGNDS CQSVNYETG LCVLFSSNA 
DVLPGALTK SQFPVFTIY AQKNCLGVK PCERAWCID 
RVQNYKLQG YTRSKLTAA TRQECLEMC LGETEFNCR 
SANYNNATG DCELSALDR LTLAGSSAF QAADGYDYM 
ENNCVEEPS KLCEFKKLN GRILKTVDS VYQDVGSVD 
ECRELCLNS PYRCHSYDY GDTGEMVCR LSHHSRATL 
ADIQDPYLE VPEAATYEL SSCYNVSID CRSGDMVAR 
IQTSKLFDG KIYAKGSPN SCVRDVNNS LEFELSMAF 
DNLECNVRK NGLGRYIND VVIQHHDKI VTSSDLGLA 
VTCQYDLTN KSVTNEVDL GVHGDIQPA LSEEVTVDS 
PNVAMKITD RSGGDVMPS AEVGDPLAL RFEIMDKNS 
PYEIFVREL VAMDGVDSS EIVLIDSDG CPTDHFIMG 
PIYKSAESG KILLSHFDA FKFPSSEVV QFRALVTPC 
MPTCEPVQC DQEESSGDL RSVVSYGRR RRRRSTDPH 
DDLLLVQSI QITDKFGFE RDANKSLES EAIFIPNES 
GVCINMAGL IVAGAVFLS AQLLIIALW TFIWQRRRS 
NKLNDGMSV SVSLPSGLS SGRTDSLCK LYESGYNHS 
RRF
    CDS sequence
    • >TC003763 RA
      atgagctgg ccgagaaga gcgcttttc gccatgttg 
gtggtagcg gcgtacagt cgcggcctc ggcgaagat 
gtggaggcg gaaatcgtc gaagctcca gataagtta 
ccggcaccg cctccgagc cctttggac gactgcgat 
ccggaactc atcggattc gagttaata accggttac 
gtgttttcc gctccggga aacgtactc gattcgata 
ccgggcact ctgatgctc accgattgt ttagaaacg 
tgccaagga aacgactcg tgccagtcg gtcaactac 
gagaccgga ctatgcgta cttttcagt tccaacgcc 
gatgtcttg ccaggagcc ctcaccaag tcgcagttc 
cccgtcttc acgatctac gctcagaag aactgtctc 
ggcgtgaag ccgtgcgag agggcttgg tgcatcgac 
agagtgcaa aactacaaa ctccaaggg tacaccagg 
agcaagctg acggctgcc acgagacaa gaatgtctc 
gaaatgtgt ctcggcgaa accgaattc aattgcaga 
tcggccaac tacaataac gcaacaggc gattgcgaa 
ctatccgcc ttggatagg ctcaccctc gctggaagt 
tccgccttc caagcggcg gacggatac gactacatg 
gagaataat tgcgtcgaa gagccctcc aaactatgc 
gaattcaag aaactcaac ggacgaata ctcaagacc 
gtcgactcg gtttaccaa gacgttggg tcggttgat 
gaatgcaga gagttgtgt ctcaactcg ccctacagg 
tgtcattcc tacgattac ggtgacacg ggagagatg 
gtctgcagg ctcagtcat cactcgcgt gccactctc 
gcagacata caggatcct tatttggag gtccccgag 
gcggcaacg tacgaattg agctcgtgc tacaacgtt 
agcatagac tgtcgctct ggagacatg gtggctcgg 
atacaaacg tccaaactc ttcgatggg aaaatctac 
gcgaaagga agtccaaac tcgtgcgtt cgcgacgtt 
aataacagc ctcgaattt gaactgtcg atggctttc 
gacaattta gaatgcaac gtgcggaaa aatggactg 
gggagatac atcaacgac gtggtgatc caacaccac 
gacaagatc gtcaccagc tcagacctc ggccttgcg 
gtcacttgc cagtacgat ttgaccaac aagagtgtg 
accaacgag gtcgatttg ggcgtccac ggggacatc 
caaccagcc ctttctgag gaagtcaca gttgactct 
cccaacgtt gccatgaag atcaccgat cgttccggc 
ggagatgtc atgccgtcg gccgaagtc ggcgatcct 
ctagcgctc cgcttcgaa atcatggac aagaacagc 
ccgtacgag atcttcgtc agggagctg gtggcaatg 
gacggcgtc gactccagc gaaatcgtg ctgatcgac 
tccgacggc tgtcccacc gaccatttc atcatgggc 
cccatctat aaatcggcc gagtccggc aagatcttg 
ttgtcgcac ttcgacgcc ttcaagttc ccctcctcc 
gaggtggtg cagttccga gcgctggtg accccctgc 
atgcccacc tgcgagccg gtgcagtgc gaccaggag 
gagtcctcc ggcgacctc cgctccgtg gtgtcgtac 
ggacgtcgt cgccgtcgc cgctccacc gacccccac 
gacgacctg ctcctggtg cagtccatc caaatcacc 
gacaaattc ggcttcgag cgcgatgcg aacaaatcg 
ctcgaatcc gaagccatc ttcatccct aacgagtcc 
ggcgtttgc ataaacatg gccggcttg atcgtcgcc 
ggcgccgtt tttctctct gcccagctg ctcatcatc 
gccttgtgg acgtttatt tggcaacgg cgaaggtcc 
aacaagctc aacgacgga atgtcggtc agcgtgagc 
ttgccctcg gggctcagt tccggccgc accgacagc 
ctctgcaag ctctacgaa tccggttat aaccactcc 
cgccgcttc taa
    mRNA sequence
    • >TC003763 RA
      ttgctcttg tttcaggtc cacactcca aacttgcaa 
acccagacc agatagacc atgagctgg ccgagaaga 
gcgcttttc gccatgttg gtggtagcg gcgtacagt 
cgcggcctc ggcgaagat gtggaggcg gaaatcgtc 
gaagctcca gataagtta ccggcaccg cctccgagc 
cctttggac gactgcgat ccggaactc atcggattc 
gagttaata accggttac gtgttttcc gctccggga 
aacgtactc gattcgata ccgggcact ctgatgctc 
accgattgt ttagaaacg tgccaagga aacgactcg 
tgccagtcg gtcaactac gagaccgga ctatgcgta 
cttttcagt tccaacgcc gatgtcttg ccaggagcc 
ctcaccaag tcgcagttc cccgtcttc acgatctac 
gctcagaag aactgtctc ggcgtgaag ccgtgcgag 
agggcttgg tgcatcgac agagtgcaa aactacaaa 
ctccaaggg tacaccagg agcaagctg acggctgcc 
acgagacaa gaatgtctc gaaatgtgt ctcggcgaa 
accgaattc aattgcaga tcggccaac tacaataac 
gcaacaggc gattgcgaa ctatccgcc ttggatagg 
ctcaccctc gctggaagt tccgccttc caagcggcg 
gacggatac gactacatg gagaataat tgcgtcgaa 
gagccctcc aaactatgc gaattcaag aaactcaac 
ggacgaata ctcaagacc gtcgactcg gtttaccaa 
gacgttggg tcggttgat gaatgcaga gagttgtgt 
ctcaactcg ccctacagg tgtcattcc tacgattac 
ggtgacacg ggagagatg gtctgcagg ctcagtcat 
cactcgcgt gccactctc gcagacata caggatcct 
tatttggag gtccccgag gcggcaacg tacgaattg 
agctcgtgc tacaacgtt agcatagac tgtcgctct 
ggagacatg gtggctcgg atacaaacg tccaaactc 
ttcgatggg aaaatctac gcgaaagga agtccaaac 
tcgtgcgtt cgcgacgtt aataacagc ctcgaattt 
gaactgtcg atggctttc gacaattta gaatgcaac 
gtgcggaaa aatggactg gggagatac atcaacgac 
gtggtgatc caacaccac gacaagatc gtcaccagc 
tcagacctc ggccttgcg gtcacttgc cagtacgat 
ttgaccaac aagagtgtg accaacgag gtcgatttg 
ggcgtccac ggggacatc caaccagcc ctttctgag 
gaagtcaca gttgactct cccaacgtt gccatgaag 
atcaccgat cgttccggc ggagatgtc atgccgtcg 
gccgaagtc ggcgatcct ctagcgctc cgcttcgaa 
atcatggac aagaacagc ccgtacgag atcttcgtc 
agggagctg gtggcaatg gacggcgtc gactccagc 
gaaatcgtg ctgatcgac tccgacggc tgtcccacc 
gaccatttc atcatgggc cccatctat aaatcggcc 
gagtccggc aagatcttg ttgtcgcac ttcgacgcc 
ttcaagttc ccctcctcc gaggtggtg cagttccga 
gcgctggtg accccctgc atgcccacc tgcgagccg 
gtgcagtgc gaccaggag gagtcctcc ggcgacctc 
cgctccgtg gtgtcgtac ggacgtcgt cgccgtcgc 
cgctccacc gacccccac gacgacctg ctcctggtg 
cagtccatc caaatcacc gacaaattc ggcttcgag 
cgcgatgcg aacaaatcg ctcgaatcc gaagccatc 
ttcatccct aacgagtcc ggcgtttgc ataaacatg 
gccggcttg atcgtcgcc ggcgccgtt tttctctct 
gcccagctg ctcatcatc gccttgtgg acgtttatt 
tggcaacgg cgaaggtcc aacaagctc aacgacgga 
atgtcggtc agcgtgagc ttgccctcg gggctcagt 
tccggccgc accgacagc ctctgcaag ctctacgaa 
tccggttat aaccactcc cgccgcttc taaccgaaa 
tcgcgtgtg atattattg tacagcgcc gtacggacc 
ctaactcgc gcgtaaaaa atacctgcg atttttcgg 
ggcgtgtcg agggcggct gtcgccgtt ttagtcgca 
gacgcgccc tggaagtct cttctgtat tgcaatgcc 
agatcttaa caccgcaaa cgtattaga ataatactc 
gtttagcta attttattt aaaaaattc tcataggaa 
aaagcttta gtaacgttt tgcaccttg tgctaagct 
tgaggttca ggtcagcgt ttaattttt tgtgaaacg 
ttactcttt ttttctaag gggcggcac gcacgaatg 
gactctagg acgatctag cgtgaatga atctatatt 
ttatttttt tataattaa atcattttt taatcactc 
aactgcaat atgggcgaa tggtgacgg atgaatgct 
atttaaaac tgcttgtcc tagtttcct gagtgcagc 
gcctggacg gttttgctc ctcgacttg caaaatcat 
ccacgctta tcgccttta aatcatttt taacaaata 
ccatatatt ttttatatt taggtaatt attataatt 
ttatactat ttataagtt atgcgctga tgtattcag 
ttataaatt ctgaatttt ctaaatata tgaattgaa 
tgaga
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