Status
Please wait ...
Gene information
General information
TC003763 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 18020620..18062976
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003763
    iBeetle screen iB_03614

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 5 (41 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    Comment: No phenotype found!
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003763 RA
      MSWPRRALF AMLVVAAYS RGLGEDVEA EIVEAPDKL 
      PAPPPSPLD DCDPELIGF ELITGYVFS APGNVLDSI 
      PGTLMLTDC LETCQGNDS CQSVNYETG LCVLFSSNA 
      DVLPGALTK SQFPVFTIY AQKNCLGVK PCERAWCID 
      RVQNYKLQG YTRSKLTAA TRQECLEMC LGETEFNCR 
      SANYNNATG DCELSALDR LTLAGSSAF QAADGYDYM 
      ENNCVEEPS KLCEFKKLN GRILKTVDS VYQDVGSVD 
      ECRELCLNS PYRCHSYDY GDTGEMVCR LSHHSRATL 
      ADIQDPYLE VPEAATYEL SSCYNVSID CRSGDMVAR 
      IQTSKLFDG KIYAKGSPN SCVRDVNNS LEFELSMAF 
      DNLECNVRK NGLGRYIND VVIQHHDKI VTSSDLGLA 
      VTCQYDLTN KSVTNEVDL GVHGDIQPA LSEEVTVDS 
      PNVAMKITD RSGGDVMPS AEVGDPLAL RFEIMDKNS 
      PYEIFVREL VAMDGVDSS EIVLIDSDG CPTDHFIMG 
      PIYKSAESG KILLSHFDA FKFPSSEVV QFRALVTPC 
      MPTCEPVQC DQEESSGDL RSVVSYGRR RRRRSTDPH 
      DDLLLVQSI QITDKFGFE RDANKSLES EAIFIPNES 
      GVCINMAGL IVAGAVFLS AQLLIIALW TFIWQRRRS 
      NKLNDGMSV SVSLPSGLS SGRTDSLCK LYESGYNHS 
      RRF
    CDS sequence
    • >TC003763 RA
      atgagctgg ccgagaaga gcgcttttc gccatgttg 
      gtggtagcg gcgtacagt cgcggcctc ggcgaagat 
      gtggaggcg gaaatcgtc gaagctcca gataagtta 
      ccggcaccg cctccgagc cctttggac gactgcgat 
      ccggaactc atcggattc gagttaata accggttac 
      gtgttttcc gctccggga aacgtactc gattcgata 
      ccgggcact ctgatgctc accgattgt ttagaaacg 
      tgccaagga aacgactcg tgccagtcg gtcaactac 
      gagaccgga ctatgcgta cttttcagt tccaacgcc 
      gatgtcttg ccaggagcc ctcaccaag tcgcagttc 
      cccgtcttc acgatctac gctcagaag aactgtctc 
      ggcgtgaag ccgtgcgag agggcttgg tgcatcgac 
      agagtgcaa aactacaaa ctccaaggg tacaccagg 
      agcaagctg acggctgcc acgagacaa gaatgtctc 
      gaaatgtgt ctcggcgaa accgaattc aattgcaga 
      tcggccaac tacaataac gcaacaggc gattgcgaa 
      ctatccgcc ttggatagg ctcaccctc gctggaagt 
      tccgccttc caagcggcg gacggatac gactacatg 
      gagaataat tgcgtcgaa gagccctcc aaactatgc 
      gaattcaag aaactcaac ggacgaata ctcaagacc 
      gtcgactcg gtttaccaa gacgttggg tcggttgat 
      gaatgcaga gagttgtgt ctcaactcg ccctacagg 
      tgtcattcc tacgattac ggtgacacg ggagagatg 
      gtctgcagg ctcagtcat cactcgcgt gccactctc 
      gcagacata caggatcct tatttggag gtccccgag 
      gcggcaacg tacgaattg agctcgtgc tacaacgtt 
      agcatagac tgtcgctct ggagacatg gtggctcgg 
      atacaaacg tccaaactc ttcgatggg aaaatctac 
      gcgaaagga agtccaaac tcgtgcgtt cgcgacgtt 
      aataacagc ctcgaattt gaactgtcg atggctttc 
      gacaattta gaatgcaac gtgcggaaa aatggactg 
      gggagatac atcaacgac gtggtgatc caacaccac 
      gacaagatc gtcaccagc tcagacctc ggccttgcg 
      gtcacttgc cagtacgat ttgaccaac aagagtgtg 
      accaacgag gtcgatttg ggcgtccac ggggacatc 
      caaccagcc ctttctgag gaagtcaca gttgactct 
      cccaacgtt gccatgaag atcaccgat cgttccggc 
      ggagatgtc atgccgtcg gccgaagtc ggcgatcct 
      ctagcgctc cgcttcgaa atcatggac aagaacagc 
      ccgtacgag atcttcgtc agggagctg gtggcaatg 
      gacggcgtc gactccagc gaaatcgtg ctgatcgac 
      tccgacggc tgtcccacc gaccatttc atcatgggc 
      cccatctat aaatcggcc gagtccggc aagatcttg 
      ttgtcgcac ttcgacgcc ttcaagttc ccctcctcc 
      gaggtggtg cagttccga gcgctggtg accccctgc 
      atgcccacc tgcgagccg gtgcagtgc gaccaggag 
      gagtcctcc ggcgacctc cgctccgtg gtgtcgtac 
      ggacgtcgt cgccgtcgc cgctccacc gacccccac 
      gacgacctg ctcctggtg cagtccatc caaatcacc 
      gacaaattc ggcttcgag cgcgatgcg aacaaatcg 
      ctcgaatcc gaagccatc ttcatccct aacgagtcc 
      ggcgtttgc ataaacatg gccggcttg atcgtcgcc 
      ggcgccgtt tttctctct gcccagctg ctcatcatc 
      gccttgtgg acgtttatt tggcaacgg cgaaggtcc 
      aacaagctc aacgacgga atgtcggtc agcgtgagc 
      ttgccctcg gggctcagt tccggccgc accgacagc 
      ctctgcaag ctctacgaa tccggttat aaccactcc 
      cgccgcttc taa
    mRNA sequence
    • >TC003763 RA
      ttgctcttg tttcaggtc cacactcca aacttgcaa 
      acccagacc agatagacc atgagctgg ccgagaaga 
      gcgcttttc gccatgttg gtggtagcg gcgtacagt 
      cgcggcctc ggcgaagat gtggaggcg gaaatcgtc 
      gaagctcca gataagtta ccggcaccg cctccgagc 
      cctttggac gactgcgat ccggaactc atcggattc 
      gagttaata accggttac gtgttttcc gctccggga 
      aacgtactc gattcgata ccgggcact ctgatgctc 
      accgattgt ttagaaacg tgccaagga aacgactcg 
      tgccagtcg gtcaactac gagaccgga ctatgcgta 
      cttttcagt tccaacgcc gatgtcttg ccaggagcc 
      ctcaccaag tcgcagttc cccgtcttc acgatctac 
      gctcagaag aactgtctc ggcgtgaag ccgtgcgag 
      agggcttgg tgcatcgac agagtgcaa aactacaaa 
      ctccaaggg tacaccagg agcaagctg acggctgcc 
      acgagacaa gaatgtctc gaaatgtgt ctcggcgaa 
      accgaattc aattgcaga tcggccaac tacaataac 
      gcaacaggc gattgcgaa ctatccgcc ttggatagg 
      ctcaccctc gctggaagt tccgccttc caagcggcg 
      gacggatac gactacatg gagaataat tgcgtcgaa 
      gagccctcc aaactatgc gaattcaag aaactcaac 
      ggacgaata ctcaagacc gtcgactcg gtttaccaa 
      gacgttggg tcggttgat gaatgcaga gagttgtgt 
      ctcaactcg ccctacagg tgtcattcc tacgattac 
      ggtgacacg ggagagatg gtctgcagg ctcagtcat 
      cactcgcgt gccactctc gcagacata caggatcct 
      tatttggag gtccccgag gcggcaacg tacgaattg 
      agctcgtgc tacaacgtt agcatagac tgtcgctct 
      ggagacatg gtggctcgg atacaaacg tccaaactc 
      ttcgatggg aaaatctac gcgaaagga agtccaaac 
      tcgtgcgtt cgcgacgtt aataacagc ctcgaattt 
      gaactgtcg atggctttc gacaattta gaatgcaac 
      gtgcggaaa aatggactg gggagatac atcaacgac 
      gtggtgatc caacaccac gacaagatc gtcaccagc 
      tcagacctc ggccttgcg gtcacttgc cagtacgat 
      ttgaccaac aagagtgtg accaacgag gtcgatttg 
      ggcgtccac ggggacatc caaccagcc ctttctgag 
      gaagtcaca gttgactct cccaacgtt gccatgaag 
      atcaccgat cgttccggc ggagatgtc atgccgtcg 
      gccgaagtc ggcgatcct ctagcgctc cgcttcgaa 
      atcatggac aagaacagc ccgtacgag atcttcgtc 
      agggagctg gtggcaatg gacggcgtc gactccagc 
      gaaatcgtg ctgatcgac tccgacggc tgtcccacc 
      gaccatttc atcatgggc cccatctat aaatcggcc 
      gagtccggc aagatcttg ttgtcgcac ttcgacgcc 
      ttcaagttc ccctcctcc gaggtggtg cagttccga 
      gcgctggtg accccctgc atgcccacc tgcgagccg 
      gtgcagtgc gaccaggag gagtcctcc ggcgacctc 
      cgctccgtg gtgtcgtac ggacgtcgt cgccgtcgc 
      cgctccacc gacccccac gacgacctg ctcctggtg 
      cagtccatc caaatcacc gacaaattc ggcttcgag 
      cgcgatgcg aacaaatcg ctcgaatcc gaagccatc 
      ttcatccct aacgagtcc ggcgtttgc ataaacatg 
      gccggcttg atcgtcgcc ggcgccgtt tttctctct 
      gcccagctg ctcatcatc gccttgtgg acgtttatt 
      tggcaacgg cgaaggtcc aacaagctc aacgacgga 
      atgtcggtc agcgtgagc ttgccctcg gggctcagt 
      tccggccgc accgacagc ctctgcaag ctctacgaa 
      tccggttat aaccactcc cgccgcttc taaccgaaa 
      tcgcgtgtg atattattg tacagcgcc gtacggacc 
      ctaactcgc gcgtaaaaa atacctgcg atttttcgg 
      ggcgtgtcg agggcggct gtcgccgtt ttagtcgca 
      gacgcgccc tggaagtct cttctgtat tgcaatgcc 
      agatcttaa caccgcaaa cgtattaga ataatactc 
      gtttagcta attttattt aaaaaattc tcataggaa 
      aaagcttta gtaacgttt tgcaccttg tgctaagct 
      tgaggttca ggtcagcgt ttaattttt tgtgaaacg 
      ttactcttt ttttctaag gggcggcac gcacgaatg 
      gactctagg acgatctag cgtgaatga atctatatt 
      ttatttttt tataattaa atcattttt taatcactc 
      aactgcaat atgggcgaa tggtgacgg atgaatgct 
      atttaaaac tgcttgtcc tagtttcct gagtgcagc 
      gcctggacg gttttgctc ctcgacttg caaaatcat 
      ccacgctta tcgccttta aatcatttt taacaaata 
      ccatatatt ttttatatt taggtaatt attataatt 
      ttatactat ttataagtt atgcgctga tgtattcag 
      ttataaatt ctgaatttt ctaaatata tgaattgaa 
      tgaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY