| General information | |||
| TC003757 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 18216351..18227076 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC003757 | ||
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03613 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
- >TC003757 RA
MLIIRNWYL NFSSMRRRS NVSFNIKTF VGITFLFLY CEYIIYYVV ISSCKWPEL NPANEDPTI AKNNYEPVK VMVLADTHL LGSRNGHWF DKLRREWQM HRAFQTAMS LFKPDLVFV LGDLTDEGL YCSDAEFEY YVKRFYNLF AVPETTKLY VAVGNHDIG FHYRVSPYL NQRFVAAFN APAVQLISV KGNHFVLVN SMALEGDGC FLCQPAEHQ LSRIEKKLK CTKGDYSGK CDSKLDIYS KPILMQHYP LYRKSDMEC DDFDAAPDP IKRERFRET WECLSKEAT TQLLNQIKP RLALSGHTH HGCTRPLPN GDGIEITLP SFSWRNKEN PNFGLGVFT PNNYAFTKC EMPKEFTVI ILYIIGVII LLLWLLCTR YKKYKLR
- >TC003757 RA
atgctgatt atcagaaat tggtatctt aattttagc agcatgaga aggcgctca aatgttagt ttcaacata aaaacgttt gtgggcatt accttttta ttcctttac tgcgagtac atcatttat tacgtcgtc ataagttct tgcaagtgg ccggagctg aaccccgct aacgaggac ccaacaatt gccaaaaac aattatgag ccggtcaaa gtcatggta cttgcagat acgcactta ttgggctcc cgaaatggg cattggttt gataaattg cgtcgcgaa tggcaaatg catagggcg tttcagact gcgatgagt ctgtttaag cccgatttg gtgtttgtt ttgggggat ttgaccgat gagggtctc tactgcagc gatgcggag tttgagtat tatgtgaag aggttctat aatttgttt gctgtgcca gagacaacc aagttgtat gtggctgtg gggaatcac gatattggt tttcactac agggttagt ccttattta aatcagagg tttgtggct gcgttcaat gccccagct gttcagttg ataagtgtt aaaggcaat cattttgtg ctagtcaat agtatggct ttggagggc gatggctgc tttttgtgc caaccagcg gaacaccag ttatctagg attgaaaaa aaattaaag tgtacaaaa ggtgactat agtgggaaa tgtgactca aaacttgac atttacagt aaaccaatt cttatgcag cattaccca ctttacaga aaatccgat atggaatgt gacgatttt gacgcagcg cctgatccg atcaaacgc gaacgtttt cgcgaaaca tgggaatgt ctcagtaaa gaagctaca acacaactt ttaaaccaa attaaaccc agattagcc ttgtcaggt catacccat catggttgc acgcgacca ctgccaaat ggtgatggg attgaaatt actttacca agttttagt tggaggaat aaagagaac cctaatttt ggacttggt gtttttact ccaaacaat tacgctttt accaaatgt gagatgcca aaagaattt actgttatc attctgtat attattggg gtgattatt ttgctactt tggttgctt tgtacgagg tataagaag tacaagttg cgctga
- >TC003757 RA
ggataagaa aataaatac ctgctattc ttttggtta atatttaaa aaaaagaca agtggattg aatagtttt agaaaattt taagacaaa aacagaaaa taccgtatc gctaacgat ttttggaaa tttggaggt taaaaaatt tggtcaaaa agtaattaa ttaaaaagg gggccacat aattagacc gcaattgag gaaaaattt ctgaagaac ctaaaatct taaaccaca aacaaaatt tatcgcaaa ataaatttc tgtcttttt cgtccaaac ttttgtagc aagaagctc atgtttgtc cataaacca gaaatacca taatacaac acaagaaga gtttattaa aagtatatt ttccccata tctcaacaa cttataatc acacagaaa ctagcattt aacttgtaa tagtctgta atagactta gaactagaa ctagttttg tcaaaggtg gcacctcga ctggctcgg cttccctga ctgtgggtt tcgtctaaa acgggtaca gttcttttt agaatcaat ataatatgc attggaacc attgtatta aaacatcag actcattaa ttgttactt gttgtgtac agctttagt attacgctt tcgttgtaa gcaaatggt tgtgcatat tataggtca ctcgtcaaa tcctggtat ttggctgtg gcttcggtg acaccaata gcgaattct agagggaga ggcacttga acatcactc gaacccaaa tgtacatac acaaaatta aattgagag gaatatatg atttatttt gtaaaaatg cagtattaa aaaccaaca gaagagcta actaatagc tgtgccaaa gtaagcaag aactgttcg cttcaacac acgaatatt accaaaagt tccaaaaag agcacattt gttttcaat tttggaatt gctgaaaag ttcacgaaa attcaatgt ctgccttca ctgcatttt gcaagcact gttgatttt gtgcgctga tgtaaataa tgttattta atttaggtc ttaagtgcg tgattatct actttacgt agaggaaaa cctagagta gaaatagag cttaaagga gtatttata cgaaaacta aattcgttg cgtctttct acagattgg tgattacaa tttagcaat aagtcgtag caaacagtt tgtcatatc ggagcatat tctaaatac aatacttgg aaacatagc attgatcaa tctgtacaa aaactgcga gatttagct gttattgtt gcaacctgc atcggctaa attaaatgt atatagata tattaggtg ctacataaa ttcgtttat ttgatgaga atttgtaaa ttttattac tctatcgac taagttacc tatttacta atgttaata actgttgtt aaatgacgt gcgatttta ataattata acgaatcag ggaataatg ttacattta ccttgaatt ggttgtttt gtttttaat gtttacgtt tgttgaata agaaaattg attgtcgtt aatgttaaa cactacagt tagccaagc tgattgctt attccatag ttatattta aaattatag actgtaatg ttttacata attcgtagc agtccttcc tttattttt tcaattgta aaattatcg acgaatgac caacaaccc attgtagtg tgccaatat acttaaaga aaacacata gatttaatt acaaataaa gcgcttcta acagtcggt tctcaaatt ggaaattct ccatcacat tttattttt atatcggtc accaactac aaatactat acaatacat ttgttacgt tttttagca atcttcttt cacttacaa aaattttaa tgattaaaa atgacacga attaaaatc ttgttgtaa caggtattt acctaatca tgcacacgt ggaggaaaa taatattaa taaccaaat tgtatataa tccaaaaat ttacaatta taaattata atatagatc tgttgtttt atcgttaga cttgaattg ttaattttg tctgatctt tatttataa atacgtaaa cttaagtga atgccacaa tcacattaa ttaaattgt tttacactg ttcatacgc aattttaat aaagaatga aaatatgta cactttttc atttatcct ttgtcatcc tcttatata ttttatttt ttttttatg ctgattatc agaaattgg tatcttaat tttagcagc atgagaagg cgctcaaat gttagtttc aacataaaa acgtttgtg ggcattacc tttttattc ctttactgc gagtacatc atttattac gtcgtcata agttcttgc aagtggccg gagctgaac cccgctaac gaggaccca acaattgcc aaaaacaat tatgagccg gtcaaagtc atggtactt gcagatacg cacttattg ggctcccga aatgggcat tggtttgat aaattgcgt cgcgaatgg caaatgcat agggcgttt cagactgcg atgagtctg tttaagccc gatttggtg tttgttttg ggggatttg accgatgag ggtctctac tgcagcgat gcggagttt gagtattat gtgaagagg ttctataat ttgtttgct gtgccagag acaaccaag ttgtatgtg gctgtgggg aatcacgat attggtttt cactacagg gttagtcct tatttaaat cagaggttt gtggctgcg ttcaatgcc ccagctgtt cagttgata agtgttaaa ggcaatcat tttgtgcta gtcaatagt atggctttg gagggcgat ggctgcttt ttgtgccaa ccagcggaa caccagtta tctaggatt gaaaaaaaa ttaaagtgt acaaaaggt gactatagt gggaaatgt gactcaaaa cttgacatt tacagtaaa ccaattctt atgcagcat tacccactt tacagaaaa tccgatatg gaatgtgac gattttgac gcagcgcct gatccgatc aaacgcgaa cgttttcgc gaaacatgg gaatgtctc agtaaagaa gctacaaca caactttta aaccaaatt aaacccaga ttagccttg tcaggtcat acccatcat ggttgcacg cgaccactg ccaaatggt gatgggatt gaaattact ttaccaagt tttagttgg aggaataaa gagaaccct aattttgga cttggtgtt tttactcca aacaattac gcttttacc aaatgtgag atgccaaaa gaatttact gttatcatt ctgtatatt attggggtg attattttg ctactttgg ttgctttgt acgaggtat aagaagtac aagttgcgc tgacgtttc tgacccctg tggggttaa ctaaggatc tgttttaaa tcatgtgga tagtaaagt ttttgttcc agttttgtg ttttttatt acattttat tattttgga ataaaatta ttttaatat t
