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Gene information
General information
TC003757 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 18216351..18227076
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003757
    iBeetle screen iB_03613

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003757 RA
      MLIIRNWYL NFSSMRRRS NVSFNIKTF VGITFLFLY 
CEYIIYYVV ISSCKWPEL NPANEDPTI AKNNYEPVK 
VMVLADTHL LGSRNGHWF DKLRREWQM HRAFQTAMS 
LFKPDLVFV LGDLTDEGL YCSDAEFEY YVKRFYNLF 
AVPETTKLY VAVGNHDIG FHYRVSPYL NQRFVAAFN 
APAVQLISV KGNHFVLVN SMALEGDGC FLCQPAEHQ 
LSRIEKKLK CTKGDYSGK CDSKLDIYS KPILMQHYP 
LYRKSDMEC DDFDAAPDP IKRERFRET WECLSKEAT 
TQLLNQIKP RLALSGHTH HGCTRPLPN GDGIEITLP 
SFSWRNKEN PNFGLGVFT PNNYAFTKC EMPKEFTVI 
ILYIIGVII LLLWLLCTR YKKYKLR
    CDS sequence
    • >TC003757 RA
      atgctgatt atcagaaat tggtatctt aattttagc 
agcatgaga aggcgctca aatgttagt ttcaacata 
aaaacgttt gtgggcatt accttttta ttcctttac 
tgcgagtac atcatttat tacgtcgtc ataagttct 
tgcaagtgg ccggagctg aaccccgct aacgaggac 
ccaacaatt gccaaaaac aattatgag ccggtcaaa 
gtcatggta cttgcagat acgcactta ttgggctcc 
cgaaatggg cattggttt gataaattg cgtcgcgaa 
tggcaaatg catagggcg tttcagact gcgatgagt 
ctgtttaag cccgatttg gtgtttgtt ttgggggat 
ttgaccgat gagggtctc tactgcagc gatgcggag 
tttgagtat tatgtgaag aggttctat aatttgttt 
gctgtgcca gagacaacc aagttgtat gtggctgtg 
gggaatcac gatattggt tttcactac agggttagt 
ccttattta aatcagagg tttgtggct gcgttcaat 
gccccagct gttcagttg ataagtgtt aaaggcaat 
cattttgtg ctagtcaat agtatggct ttggagggc 
gatggctgc tttttgtgc caaccagcg gaacaccag 
ttatctagg attgaaaaa aaattaaag tgtacaaaa 
ggtgactat agtgggaaa tgtgactca aaacttgac 
atttacagt aaaccaatt cttatgcag cattaccca 
ctttacaga aaatccgat atggaatgt gacgatttt 
gacgcagcg cctgatccg atcaaacgc gaacgtttt 
cgcgaaaca tgggaatgt ctcagtaaa gaagctaca 
acacaactt ttaaaccaa attaaaccc agattagcc 
ttgtcaggt catacccat catggttgc acgcgacca 
ctgccaaat ggtgatggg attgaaatt actttacca 
agttttagt tggaggaat aaagagaac cctaatttt 
ggacttggt gtttttact ccaaacaat tacgctttt 
accaaatgt gagatgcca aaagaattt actgttatc 
attctgtat attattggg gtgattatt ttgctactt 
tggttgctt tgtacgagg tataagaag tacaagttg 
cgctga
    mRNA sequence
    • >TC003757 RA
      ggataagaa aataaatac ctgctattc ttttggtta 
atatttaaa aaaaagaca agtggattg aatagtttt 
agaaaattt taagacaaa aacagaaaa taccgtatc 
gctaacgat ttttggaaa tttggaggt taaaaaatt 
tggtcaaaa agtaattaa ttaaaaagg gggccacat 
aattagacc gcaattgag gaaaaattt ctgaagaac 
ctaaaatct taaaccaca aacaaaatt tatcgcaaa 
ataaatttc tgtcttttt cgtccaaac ttttgtagc 
aagaagctc atgtttgtc cataaacca gaaatacca 
taatacaac acaagaaga gtttattaa aagtatatt 
ttccccata tctcaacaa cttataatc acacagaaa 
ctagcattt aacttgtaa tagtctgta atagactta 
gaactagaa ctagttttg tcaaaggtg gcacctcga 
ctggctcgg cttccctga ctgtgggtt tcgtctaaa 
acgggtaca gttcttttt agaatcaat ataatatgc 
attggaacc attgtatta aaacatcag actcattaa 
ttgttactt gttgtgtac agctttagt attacgctt 
tcgttgtaa gcaaatggt tgtgcatat tataggtca 
ctcgtcaaa tcctggtat ttggctgtg gcttcggtg 
acaccaata gcgaattct agagggaga ggcacttga 
acatcactc gaacccaaa tgtacatac acaaaatta 
aattgagag gaatatatg atttatttt gtaaaaatg 
cagtattaa aaaccaaca gaagagcta actaatagc 
tgtgccaaa gtaagcaag aactgttcg cttcaacac 
acgaatatt accaaaagt tccaaaaag agcacattt 
gttttcaat tttggaatt gctgaaaag ttcacgaaa 
attcaatgt ctgccttca ctgcatttt gcaagcact 
gttgatttt gtgcgctga tgtaaataa tgttattta 
atttaggtc ttaagtgcg tgattatct actttacgt 
agaggaaaa cctagagta gaaatagag cttaaagga 
gtatttata cgaaaacta aattcgttg cgtctttct 
acagattgg tgattacaa tttagcaat aagtcgtag 
caaacagtt tgtcatatc ggagcatat tctaaatac 
aatacttgg aaacatagc attgatcaa tctgtacaa 
aaactgcga gatttagct gttattgtt gcaacctgc 
atcggctaa attaaatgt atatagata tattaggtg 
ctacataaa ttcgtttat ttgatgaga atttgtaaa 
ttttattac tctatcgac taagttacc tatttacta 
atgttaata actgttgtt aaatgacgt gcgatttta 
ataattata acgaatcag ggaataatg ttacattta 
ccttgaatt ggttgtttt gtttttaat gtttacgtt 
tgttgaata agaaaattg attgtcgtt aatgttaaa 
cactacagt tagccaagc tgattgctt attccatag 
ttatattta aaattatag actgtaatg ttttacata 
attcgtagc agtccttcc tttattttt tcaattgta 
aaattatcg acgaatgac caacaaccc attgtagtg 
tgccaatat acttaaaga aaacacata gatttaatt 
acaaataaa gcgcttcta acagtcggt tctcaaatt 
ggaaattct ccatcacat tttattttt atatcggtc 
accaactac aaatactat acaatacat ttgttacgt 
tttttagca atcttcttt cacttacaa aaattttaa 
tgattaaaa atgacacga attaaaatc ttgttgtaa 
caggtattt acctaatca tgcacacgt ggaggaaaa 
taatattaa taaccaaat tgtatataa tccaaaaat 
ttacaatta taaattata atatagatc tgttgtttt 
atcgttaga cttgaattg ttaattttg tctgatctt 
tatttataa atacgtaaa cttaagtga atgccacaa 
tcacattaa ttaaattgt tttacactg ttcatacgc 
aattttaat aaagaatga aaatatgta cactttttc 
atttatcct ttgtcatcc tcttatata ttttatttt 
ttttttatg ctgattatc agaaattgg tatcttaat 
tttagcagc atgagaagg cgctcaaat gttagtttc 
aacataaaa acgtttgtg ggcattacc tttttattc 
ctttactgc gagtacatc atttattac gtcgtcata 
agttcttgc aagtggccg gagctgaac cccgctaac 
gaggaccca acaattgcc aaaaacaat tatgagccg 
gtcaaagtc atggtactt gcagatacg cacttattg 
ggctcccga aatgggcat tggtttgat aaattgcgt 
cgcgaatgg caaatgcat agggcgttt cagactgcg 
atgagtctg tttaagccc gatttggtg tttgttttg 
ggggatttg accgatgag ggtctctac tgcagcgat 
gcggagttt gagtattat gtgaagagg ttctataat 
ttgtttgct gtgccagag acaaccaag ttgtatgtg 
gctgtgggg aatcacgat attggtttt cactacagg 
gttagtcct tatttaaat cagaggttt gtggctgcg 
ttcaatgcc ccagctgtt cagttgata agtgttaaa 
ggcaatcat tttgtgcta gtcaatagt atggctttg 
gagggcgat ggctgcttt ttgtgccaa ccagcggaa 
caccagtta tctaggatt gaaaaaaaa ttaaagtgt 
acaaaaggt gactatagt gggaaatgt gactcaaaa 
cttgacatt tacagtaaa ccaattctt atgcagcat 
tacccactt tacagaaaa tccgatatg gaatgtgac 
gattttgac gcagcgcct gatccgatc aaacgcgaa 
cgttttcgc gaaacatgg gaatgtctc agtaaagaa 
gctacaaca caactttta aaccaaatt aaacccaga 
ttagccttg tcaggtcat acccatcat ggttgcacg 
cgaccactg ccaaatggt gatgggatt gaaattact 
ttaccaagt tttagttgg aggaataaa gagaaccct 
aattttgga cttggtgtt tttactcca aacaattac 
gcttttacc aaatgtgag atgccaaaa gaatttact 
gttatcatt ctgtatatt attggggtg attattttg 
ctactttgg ttgctttgt acgaggtat aagaagtac 
aagttgcgc tgacgtttc tgacccctg tggggttaa 
ctaaggatc tgttttaaa tcatgtgga tagtaaagt 
ttttgttcc agttttgtg ttttttatt acattttat 
tattttgga ataaaatta ttttaatat t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY