Status
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Gene information
General information
TC003757 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 18216351..18227076
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003757
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03613

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003757 RA
      MLIIRNWYL NFSSMRRRS NVSFNIKTF VGITFLFLY 
      CEYIIYYVV ISSCKWPEL NPANEDPTI AKNNYEPVK 
      VMVLADTHL LGSRNGHWF DKLRREWQM HRAFQTAMS 
      LFKPDLVFV LGDLTDEGL YCSDAEFEY YVKRFYNLF 
      AVPETTKLY VAVGNHDIG FHYRVSPYL NQRFVAAFN 
      APAVQLISV KGNHFVLVN SMALEGDGC FLCQPAEHQ 
      LSRIEKKLK CTKGDYSGK CDSKLDIYS KPILMQHYP 
      LYRKSDMEC DDFDAAPDP IKRERFRET WECLSKEAT 
      TQLLNQIKP RLALSGHTH HGCTRPLPN GDGIEITLP 
      SFSWRNKEN PNFGLGVFT PNNYAFTKC EMPKEFTVI 
      ILYIIGVII LLLWLLCTR YKKYKLR
    CDS sequence
    • >TC003757 RA
      atgctgatt atcagaaat tggtatctt aattttagc 
      agcatgaga aggcgctca aatgttagt ttcaacata 
      aaaacgttt gtgggcatt accttttta ttcctttac 
      tgcgagtac atcatttat tacgtcgtc ataagttct 
      tgcaagtgg ccggagctg aaccccgct aacgaggac 
      ccaacaatt gccaaaaac aattatgag ccggtcaaa 
      gtcatggta cttgcagat acgcactta ttgggctcc 
      cgaaatggg cattggttt gataaattg cgtcgcgaa 
      tggcaaatg catagggcg tttcagact gcgatgagt 
      ctgtttaag cccgatttg gtgtttgtt ttgggggat 
      ttgaccgat gagggtctc tactgcagc gatgcggag 
      tttgagtat tatgtgaag aggttctat aatttgttt 
      gctgtgcca gagacaacc aagttgtat gtggctgtg 
      gggaatcac gatattggt tttcactac agggttagt 
      ccttattta aatcagagg tttgtggct gcgttcaat 
      gccccagct gttcagttg ataagtgtt aaaggcaat 
      cattttgtg ctagtcaat agtatggct ttggagggc 
      gatggctgc tttttgtgc caaccagcg gaacaccag 
      ttatctagg attgaaaaa aaattaaag tgtacaaaa 
      ggtgactat agtgggaaa tgtgactca aaacttgac 
      atttacagt aaaccaatt cttatgcag cattaccca 
      ctttacaga aaatccgat atggaatgt gacgatttt 
      gacgcagcg cctgatccg atcaaacgc gaacgtttt 
      cgcgaaaca tgggaatgt ctcagtaaa gaagctaca 
      acacaactt ttaaaccaa attaaaccc agattagcc 
      ttgtcaggt catacccat catggttgc acgcgacca 
      ctgccaaat ggtgatggg attgaaatt actttacca 
      agttttagt tggaggaat aaagagaac cctaatttt 
      ggacttggt gtttttact ccaaacaat tacgctttt 
      accaaatgt gagatgcca aaagaattt actgttatc 
      attctgtat attattggg gtgattatt ttgctactt 
      tggttgctt tgtacgagg tataagaag tacaagttg 
      cgctga
    mRNA sequence
    • >TC003757 RA
      ggataagaa aataaatac ctgctattc ttttggtta 
      atatttaaa aaaaagaca agtggattg aatagtttt 
      agaaaattt taagacaaa aacagaaaa taccgtatc 
      gctaacgat ttttggaaa tttggaggt taaaaaatt 
      tggtcaaaa agtaattaa ttaaaaagg gggccacat 
      aattagacc gcaattgag gaaaaattt ctgaagaac 
      ctaaaatct taaaccaca aacaaaatt tatcgcaaa 
      ataaatttc tgtcttttt cgtccaaac ttttgtagc 
      aagaagctc atgtttgtc cataaacca gaaatacca 
      taatacaac acaagaaga gtttattaa aagtatatt 
      ttccccata tctcaacaa cttataatc acacagaaa 
      ctagcattt aacttgtaa tagtctgta atagactta 
      gaactagaa ctagttttg tcaaaggtg gcacctcga 
      ctggctcgg cttccctga ctgtgggtt tcgtctaaa 
      acgggtaca gttcttttt agaatcaat ataatatgc 
      attggaacc attgtatta aaacatcag actcattaa 
      ttgttactt gttgtgtac agctttagt attacgctt 
      tcgttgtaa gcaaatggt tgtgcatat tataggtca 
      ctcgtcaaa tcctggtat ttggctgtg gcttcggtg 
      acaccaata gcgaattct agagggaga ggcacttga 
      acatcactc gaacccaaa tgtacatac acaaaatta 
      aattgagag gaatatatg atttatttt gtaaaaatg 
      cagtattaa aaaccaaca gaagagcta actaatagc 
      tgtgccaaa gtaagcaag aactgttcg cttcaacac 
      acgaatatt accaaaagt tccaaaaag agcacattt 
      gttttcaat tttggaatt gctgaaaag ttcacgaaa 
      attcaatgt ctgccttca ctgcatttt gcaagcact 
      gttgatttt gtgcgctga tgtaaataa tgttattta 
      atttaggtc ttaagtgcg tgattatct actttacgt 
      agaggaaaa cctagagta gaaatagag cttaaagga 
      gtatttata cgaaaacta aattcgttg cgtctttct 
      acagattgg tgattacaa tttagcaat aagtcgtag 
      caaacagtt tgtcatatc ggagcatat tctaaatac 
      aatacttgg aaacatagc attgatcaa tctgtacaa 
      aaactgcga gatttagct gttattgtt gcaacctgc 
      atcggctaa attaaatgt atatagata tattaggtg 
      ctacataaa ttcgtttat ttgatgaga atttgtaaa 
      ttttattac tctatcgac taagttacc tatttacta 
      atgttaata actgttgtt aaatgacgt gcgatttta 
      ataattata acgaatcag ggaataatg ttacattta 
      ccttgaatt ggttgtttt gtttttaat gtttacgtt 
      tgttgaata agaaaattg attgtcgtt aatgttaaa 
      cactacagt tagccaagc tgattgctt attccatag 
      ttatattta aaattatag actgtaatg ttttacata 
      attcgtagc agtccttcc tttattttt tcaattgta 
      aaattatcg acgaatgac caacaaccc attgtagtg 
      tgccaatat acttaaaga aaacacata gatttaatt 
      acaaataaa gcgcttcta acagtcggt tctcaaatt 
      ggaaattct ccatcacat tttattttt atatcggtc 
      accaactac aaatactat acaatacat ttgttacgt 
      tttttagca atcttcttt cacttacaa aaattttaa 
      tgattaaaa atgacacga attaaaatc ttgttgtaa 
      caggtattt acctaatca tgcacacgt ggaggaaaa 
      taatattaa taaccaaat tgtatataa tccaaaaat 
      ttacaatta taaattata atatagatc tgttgtttt 
      atcgttaga cttgaattg ttaattttg tctgatctt 
      tatttataa atacgtaaa cttaagtga atgccacaa 
      tcacattaa ttaaattgt tttacactg ttcatacgc 
      aattttaat aaagaatga aaatatgta cactttttc 
      atttatcct ttgtcatcc tcttatata ttttatttt 
      ttttttatg ctgattatc agaaattgg tatcttaat 
      tttagcagc atgagaagg cgctcaaat gttagtttc 
      aacataaaa acgtttgtg ggcattacc tttttattc 
      ctttactgc gagtacatc atttattac gtcgtcata 
      agttcttgc aagtggccg gagctgaac cccgctaac 
      gaggaccca acaattgcc aaaaacaat tatgagccg 
      gtcaaagtc atggtactt gcagatacg cacttattg 
      ggctcccga aatgggcat tggtttgat aaattgcgt 
      cgcgaatgg caaatgcat agggcgttt cagactgcg 
      atgagtctg tttaagccc gatttggtg tttgttttg 
      ggggatttg accgatgag ggtctctac tgcagcgat 
      gcggagttt gagtattat gtgaagagg ttctataat 
      ttgtttgct gtgccagag acaaccaag ttgtatgtg 
      gctgtgggg aatcacgat attggtttt cactacagg 
      gttagtcct tatttaaat cagaggttt gtggctgcg 
      ttcaatgcc ccagctgtt cagttgata agtgttaaa 
      ggcaatcat tttgtgcta gtcaatagt atggctttg 
      gagggcgat ggctgcttt ttgtgccaa ccagcggaa 
      caccagtta tctaggatt gaaaaaaaa ttaaagtgt 
      acaaaaggt gactatagt gggaaatgt gactcaaaa 
      cttgacatt tacagtaaa ccaattctt atgcagcat 
      tacccactt tacagaaaa tccgatatg gaatgtgac 
      gattttgac gcagcgcct gatccgatc aaacgcgaa 
      cgttttcgc gaaacatgg gaatgtctc agtaaagaa 
      gctacaaca caactttta aaccaaatt aaacccaga 
      ttagccttg tcaggtcat acccatcat ggttgcacg 
      cgaccactg ccaaatggt gatgggatt gaaattact 
      ttaccaagt tttagttgg aggaataaa gagaaccct 
      aattttgga cttggtgtt tttactcca aacaattac 
      gcttttacc aaatgtgag atgccaaaa gaatttact 
      gttatcatt ctgtatatt attggggtg attattttg 
      ctactttgg ttgctttgt acgaggtat aagaagtac 
      aagttgcgc tgacgtttc tgacccctg tggggttaa 
      ctaaggatc tgttttaaa tcatgtgga tagtaaagt 
      ttttgttcc agttttgtg ttttttatt acattttat 
      tattttgga ataaaatta ttttaatat t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY