Status
Please wait ...
Gene information
General information
TC032535
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003755,TC003756
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 18277126..18376768
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032535
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03612

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032535 RA
      MRAGEEYSE LADLGSSYL GGGRYGGTA WTRTPGRAR 
      TNTMPAEAE DVLDPNGSP RGHSRSRSG LYYSPPGTS 
      YTIVERPSS VMHHHSSRD YNTQHYNTL TSPRGTYLG 
      SNPNFSTAS RTTPSNNKK RPISPEQVL RLFGSNNQS 
      NTKLNPERP RRSPASSPP STTHQVNYR TPYGPSIHE 
      LATRTVTMV RDPQDGTHG FGICVKGGK DAGVGVYIS 
      RVEEGSVAE RAGLRPGDS ILEVNGTPF TGISHEEAL 
      KVGQSLGLM IRGGVEYNL GIFITGVDK DSVADRAGL 
      MVGDQILEV NGQSFMDVT HDEAVAQLK YHKRMSLLV 
      RDVGKVPHS CTAYDREWD LCSPGSKAA QTTRKWAAA 
      LQMVEEKAR CLLSRPEFT TLCYYTDEY ASRHMTIDA 
      FVQVMTELL NTPEKYTLM TELREIVAI EDRVRFDEL 
      VYRRDVETS RIRDPREHD YRHSRRKGI PIPDPNQPA 
      NFSTYHEEY GPVIEPPPG VSISNEDDY RSPSEDSGL 
      GMGISDRIQ RTRPPSATG KIVTALNDE DHQTQEYVS 
      GDEENPPGT RSRRHSSPG ESRSGSTTA LHQQDYPDS 
      VSINVQ
    CDS sequence
    • >TC032535 RA
      atgcgcgct ggggaggaa tactctgag ctggctgat 
      ctaggcagc tcatattta ggaggaggc cgctacgga 
      ggcaccgct tggactcgc accccggga agggcgcgg 
      actaacaca atgcctgcg gaggccgag gatgtgctc 
      gaccccaac ggcagcccc aggggacac tccaggagc 
      cgctccggg ctgtactac tctcctccc ggcacttcc 
      tacactatc gtcgagagg ccgtcgtct gtgatgcac 
      caccactct tccagggac tacaatacg cagcactat 
      aacaccctc acgagtccc aggggtacg tatctgggg 
      tcaaatccg aatttcagc acggcaagc agaacaaca 
      ccaagtaat aacaagaaa cggccgatt tctccagaa 
      caagttctt cgtttattc ggtagcaat aatcaatca 
      aacactaaa ttaaatcca gaaaggccg cgaaggtct 
      ccagctagc agtcctcct tctaccaca catcaagtt 
      aattataga actccctat ggccctagc atccacgag 
      ctggcaacg aggaccgtc acgatggtc agggaccca 
      caggatggg acccatggg ttcgggatt tgtgtcaaa 
      ggagggaaa gacgcaggg gtgggtgtg tacatctcg 
      agggtggaa gaaggcagc gtggcagaa cgtgcagga 
      ttgaggccg ggcgactca atattggaa gttaacgga 
      acaccattt acgggcatc tcacacgag gaagcttta 
      aaggtaggc caaagtctc ggcttgatg atacgaggc 
      ggcgttgag tataatctt gggattttt atcaccgga 
      gttgataaa gattcagtg gcagacagg gccggactt 
      atggtcggt gatcaaatt ttagaagtg aacggacaa 
      tcttttatg gatgtgaca catgacgaa gcggtggca 
      cagctcaaa tatcacaaa aggatgagt cttttggta 
      agggatgtc gggaaggtt ccgcactca tgcactgcc 
      tacgatagg gaatgggat ttgtgcagt ccagggagc 
      aaagcagcg caaacaacg agaaaatgg gcggcggcc 
      cttcagatg gtggaggaa aaggcgcga tgtcttctc 
      tcgcgtccg gaattcaca accctttgc tattacacg 
      gatgagtac gcgtccaga catatgacc atagatgcc 
      ttcgtacaa gtcatgact gaacttctc aacactccc 
      gaaaagtat actcttatg accgaacta agggagata 
      gtagccatc gaagatcgc gtaagattc gacgaatta 
      gtgtacaga agggatgtc gaaacttct agaataaga 
      gatcctaga gagcacgat tacagacac tcgcggaga 
      aaaggaata cccatcccg gatcccaac caaccggca 
      aacttttca acttatcac gaggagtac ggtccagta 
      atagagcca cctcctggt gtaagtata agtaacgaa 
      gacgattat cgctctccc agtgaggat tcaggttta 
      ggaatggga atttcagat agaattcag agaactcgc 
      ccaccaagt gccacagga aaaatagta accgcttta 
      aacgacgaa gatcaccaa actcaggaa tatgtcagc 
      ggtgacgag gaaaatcct ccggggacc cggagtcgg 
      agacactcc agtccgggg gaatccagg tccgggagc 
      accacggca ctacatcag caggattat ccagattct 
      gtgagtatt aacgtccag tga
    mRNA sequence
    • >TC032535 RA
      atgcaatta ttttgatgg aaaagtgat tcgcaaaaa 
      gacgatgtt ttaacgcaa aaagcatat ttccggtga 
      acaaatcac caattcgtt tccttctct tctcaaagc 
      tgtacgaca gaaatgtgg gtttggccc tcaaattaa 
      acaaatggg cttctgagg gactgcggc tttggtccc 
      tgacctaac tcattcatt ttcataatt ggtgaaagt 
      ggaacattt atccggttg ctaaaattc gaatttcgg 
      ggtgcaaag atggtgatg tgatgtaac tattttttt 
      attttcttt ttttttaat atattgtgc atcatgctc 
      cttcgagga ataatcgtt tccttgtgt gggatacga 
      tcaactaac agctaacat gaggatttg tatacaaat 
      tctaacgta ctcgtgacg attgcacgt tggctagga 
      agtagcccg agggatgcg cgctgggga ggaatactc 
      tgagctggc tgatctagg cagctcata tttaggagg 
      aggccgcta cggaggcac cgcttggac tcgcacccc 
      gggaagggc gcggactaa cacaatgcc tgcggaggc 
      cgaggatgt gctcgaccc caacggcag ccccagggg 
      acactccag gagccgctc cgggctgta ctactctcc 
      tcccggcac ttcctacac tatcgtcga gaggccgtc 
      gtctgtgat gcaccacca ctcttccag ggactacaa 
      tacgcagca ctataacac cctcacgag tcccagggg 
      tacgtatct ggggtcaaa tccgaattt cagcacggc 
      aagcagaac aacaccaag taataacaa gaaacggcc 
      gatttctcc agaacaagt tcttcgttt attcggtag 
      caataatca atcaaacac taaattaaa tccagaaag 
      gccgcgaag gtctccagc tagcagtcc tccttctac 
      cacacatca agttaatta tagaactcc ctatggccc 
      tagcatcca cgagctggc aacgaggac cgtcacgat 
      ggtcaggga cccacagga tgggaccca tgggttcgg 
      gatttgtgt caaaggagg gaaagacgc aggggtggg 
      tgtgtacat ctcgagggt ggaagaagg cagcgtggc 
      agaacgtgc aggattgag gccgggcga ctcaatatt 
      ggaagttaa cggaacacc atttacggg catctcaca 
      cgaggaagc tttaaaggt aggccaaag tctcggctt 
      gatgatacg aggcggcgt tgagtataa tcttgggat 
      ttttatcac cggagttga taaagattc agtggcaga 
      cagggccgg acttatggt cggtgatca aattttaga 
      agtgaacgg acaatcttt tatggatgt gacacatga 
      cgaagcggt ggcacagct caaatatca caaaaggat 
      gagtctttt ggtaaggga tgtcgggaa ggttccgca 
      ctcatgcac tgcctacga tagggaatg ggatttgtg 
      cagtccagg gagcaaagc agcgcaaac aacgagaaa 
      atgggcggc ggcccttca gatggtgga ggaaaaggc 
      gcgatgtct tctctcgcg tccggaatt cacaaccct 
      ttgctatta cacggatga gtacgcgtc cagacatat 
      gaccataga tgccttcgt acaagtcat gactgaact 
      tctcaacac tcccgaaaa gtatactct tatgaccga 
      actaaggga gatagtagc catcgaaga tcgcgtaag 
      attcgacga attagtgta cagaaggga tgtcgaaac 
      ttctagaat aagagatcc tagagagca cgattacag 
      acactcgcg gagaaaagg aatacccat cccggatcc 
      caaccaacc ggcaaactt ttcaactta tcacgagga 
      gtacggtcc agtaataga gccacctcc tggtgtaag 
      tataagtaa cgaagacga ttatcgctc tcccagtga 
      ggattcagg tttaggaat gggaatttc agatagaat 
      tcagagaac tcgcccacc aagtgccac aggaaaaat 
      agtaaccgc tttaaacga cgaagatca ccaaactca 
      ggaatatgt cagcggtga cgaggaaaa tcctccggg 
      gacccggag tcggagaca ctccagtcc gggggaatc 
      caggtccgg gagcaccac ggcactaca tcagcagga 
      ttatccaga ttctgtgag tattaacgt ccagtgagt 
      ttttatatc tctaaacat acagtgttt taacgtcca 
      aatactggg attatttta ataaaatat atgttggct 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY