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Gene information
General information
TC003731 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 13999272..14004166
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003731
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03608

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003731 RA
      MANGPIAER NRDATIYVG GLDDKVTES LLWELFVQS 
      GPLVNVHMP KDRVTMMHQ GYGFVEFMG EEDADYAIK 
      IMNMIKLYG KPIRVNKAS AHQKNLDVG ANIFIGNLD 
      PEVDEKLLY DTFSAFGVI LQTPKIMRD PDTGNSKGF 
      AFINFASFE ASDASIEAM NGQYLCNRP ISVSYAFKK 
      DSKGERHGS AAERLLAAQ NPLSQADRP HQLFADAPP 
      MGMMPPGMT LAPPPPPVI LAGMMPPPP PTPASMQPP 
      PPPPVPPPS SFPSGIPPP PLPPSQPPL PPGAPPPPP 
      TSAAGARPP LPPMPASTQ ASGAPRMIP PPWQGGGGL 
      PPPPPGPPN SAAPPTSGA PPFPGCFPP PPPPGGRPP 
      NWRPPVNFG GGRPPFPPR GPPPPPPNN FSENNW
    CDS sequence
    • >TC003731 RA
      atggctaat ggaccgatt gctgaacgc aacagagat 
      gcaacaatt tacgttggt ggtttagat gataaagtt 
      acagaatcg ttattatgg gaactgttc gtccaatct 
      ggccccttg gtcaacgtt cacatgccc aaagaccgc 
      gtaacaatg atgcaccaa ggctatggt tttgtggag 
      ttcatgggt gaagaagat gctgattat gccataaaa 
      ataatgaac atgataaag ctttatgga aagccaata 
      agagtgaat aaagcatca gcccatcag aagaacttg 
      gacgtgggg gcgaatatt tttatcggg aatttggac 
      cccgaagtg gatgagaag ctgctttat gatacattt 
      tcggcgttt ggtgttata cttcaaaca cctaagata 
      atgagagac cccgacact ggcaattcc aaaggcttc 
      gctttcatc aactttgca agtttcgaa gcatctgac 
      gcctcaatc gaagcaatg aacggccaa tacctctgc 
      aaccgcccc atttccgtc tcctacgcc ttcaagaaa 
      gactccaaa ggcgaacgt cacggctcc gcagccgag 
      cgtctcctc gcagcccaa aaccccctc tcccaagcc 
      gaccgcccc catcaacta ttcgccgac gcccctccc 
      atgggcatg atgccccca ggaatgact ctcgctccg 
      cccccacct cccgtaatc ctcgctgga atgatgcct 
      ccaccaccc cccactccg gcaagtatg caacctcct 
      ccaccccca ccagttcct ccacccagt tctttcccc 
      tcggggatc ccccctccg ccattacct ccctcccaa 
      ccgcccttg ccaccaggt gcacctcca ccgcctcca 
      acctccgcc gctggagca cggccccct ctgccacca 
      atgcctgcc tcaacgcaa gcttcaggc gcccccagg 
      atgatacca cctccgtgg caaggtgga ggtgggtta 
      ccgccacca ccacctgga ccgccaaac tcggcagcg 
      cccccaacg tcaggtgca ccgccgttc ccgggatgt 
      ttcccgccg ccaccgcct ccagggggg cggcccccg 
      aattggagg ccgccggtt aattttggt ggagggaga 
      ccaccgttc ccaccaagg ggacctcca ccaccacct 
      ccgaataat tttagcgaa aataattgg taa
    mRNA sequence
    • >TC003731 RA
      gcggccggc tttaaagat tcttacaag caaagaaaa 
      gtttgcgtc taaggtttg cgtatttgg ttcgtttat 
      ttatttaat taaataagc attctttta tcaagcgat 
      gaagtaaaa ttttgctat taatacatt gagatatat 
      ccatttcat aatcatggc taatggacc gattgctga 
      acgcaacag agatgcaac aatttacgt tggtggttt 
      agatgataa agttacaga atcgttatt atgggaact 
      gttcgtcca atctggccc cttggtcaa cgttcacat 
      gcccaaaga ccgcgtaac aatgatgca ccaaggcta 
      tggttttgt ggagttcat gggtgaaga agatgctga 
      ttatgccat aaaaataat gaacatgat aaagcttta 
      tggaaagcc aataagagt gaataaagc atcagccca 
      tcagaagaa cttggacgt gggggcgaa tatttttat 
      cgggaattt ggaccccga agtggatga gaagctgct 
      ttatgatac attttcggc gtttggtgt tatacttca 
      aacacctaa gataatgag agaccccga cactggcaa 
      ttccaaagg cttcgcttt catcaactt tgcaagttt 
      cgaagcatc tgacgcctc aatcgaagc aatgaacgg 
      ccaatacct ctgcaaccg ccccatttc cgtctccta 
      cgccttcaa gaaagactc caaaggcga acgtcacgg 
      ctccgcagc cgagcgtct cctcgcagc ccaaaaccc 
      cctctccca agccgaccg cccccatca actattcgc 
      cgacgcccc tcccatggg catgatgcc cccaggaat 
      gactctcgc tccgccccc acctcccgt aatcctcgc 
      tggaatgat gcctccacc accccccac tccggcaag 
      tatgcaacc tcctccacc cccaccagt tcctccacc 
      cagttcttt cccctcggg gatcccccc tccgccatt 
      acctccctc ccaaccgcc cttgccacc aggtgcacc 
      tccaccgcc tccaacctc cgccgctgg agcacggcc 
      ccctctgcc accaatgcc tgcctcaac gcaagcttc 
      aggcgcccc caggatgat accacctcc gtggcaagg 
      tggaggtgg gttaccgcc accaccacc tggaccgcc 
      aaactcggc agcgccccc aacgtcagg tgcaccgcc 
      gttcccggg atgtttccc gccgccacc gcctccagg 
      ggggcggcc cccgaattg gaggccgcc ggttaattt 
      tggtggagg gagaccacc gttcccacc aaggggacc 
      tccaccacc acctccgaa taattttag cgaaaataa 
      ttggtaaaa acaataata caataaagt caaggtgaa 
      tttttttat ttcgttttg tgtcttgtt ttttggatt 
      ttcctatta ttatccttt ttattattg tcactattt 
      ccagtaaat ggtattgtg gtaccaggg gcttgatat 
      cctaattcc tcaaaactt aatgtttct agtgaaagt 
      agaatatgt atcattggc aattttcgt ttaacaact 
      atttggtct aaatcagga ataactgga tcccaaaat 
      tcttctact ctgcaaaat tgtaaaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY