Status
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Gene information
General information
TC003718 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 13637264..13648735
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003718
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03607

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    remarks: later than schedule, hours 48 remarks: later than schedule, hours 48 remarks: later than schedule, hours 48
    Pupal morphology
    remarks: later than schedule, hours 48
    Adult morphology
    remarks: later than schedule, hours 48 remarks: later than schedule, hours 48
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 remarks: later than schedule, hours 48 (egglay)
    remarks: later than schedule, hours 48
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    remarks: later than schedule, hours 48
    remarks - cuticles: later than schedule, hours 48
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003718 RA
      MSDEELEGD VGSLEIKGI IGFDGTTIK GFIIHPDEK 
      HVVYPLGNK VTIQDWTTK AQVFLSGHT NTISAIDVS 
      RSGKYIASG QINHIGFKA RVIIWDFES HTIISQHEL 
      HKVRVEAVA FSNNDNYLF SIGGRDCGS LVVWDVTQG 
      QVICGSAVA HGIQGEATA ILSMNRRGP CFMTAGDNH 
      LAVWTVDKE ARKVHSLDV STGKIKRAI LCMDVNERD 
      EVCYCGTAT GDVLKIRLN YHHDPDILI PAKHPMTVG 
      CFTRISKKK LPAGKVDLY SLGIKSIRR LFAGPLLIG 
      AGDGTIELV EETKCKGGG DKDSRVKVP SVPTLRCLK 
      STHVYSAVT SLQLMKKDT VILAGTINC EIYQIDMAT 
      FDTVLFVTC HTSTIYDVA FPYNFSDVF ATASKNDVR 
      VWSIKTHQE LLRIQVPNF SASSVLFSH DGKSILTAW 
      NDGIIRSFT PLTGRLIYA ILNAHNKGV SALAITHEG 
      DVLVSGGCE GQVRMWSVT PYRQQLICT LKEHKAPVS 
      AVDINKAGT EAVSASTDG TCIIWDLVR QCRRHIMFG 
      TTLFMCARY YPTDVQVLT GGSDRKLSY WEVLDGTLV 
      REVEGSESG AINALDISL DGNIFVTGG NDQIVKLWN 
      YQLGIATHI GIGHSAVIT AARFSPDQK YIVTTSGSG 
      SIFIWKCPQ DLIQPSKVK IAEEAEPPK EQKSETKDE 
      VIQDLPTAR SDHSKEGDQ KCVCRCPCV KKPVCEENG 
      GMGDGIKSP EAKASPKND TSDKKNGSV NASPKSVSS 
      SGKKNGSVT GSGRKN
    CDS sequence
    • >TC003718 RA
      atgtctgat gaagaactt gaaggggat gttgggagt 
      cttgaaatt aaaggaatt attggattt gatggaact 
      acaattaag ggatttatc atccatcct gatgaaaaa 
      cacgtcgtg tatcctttg ggaaataaa gtcaccatt 
      caggattgg accactaaa gcccaagtt tttctcagt 
      ggtcatact aacactatt tctgccatc gatgtttcc 
      agatccgga aaatatatt gctagcggt caaattaac 
      cacattgga ttcaaagcc cgcgtcata atttgggac 
      ttcgaaagc cacaccatc atctcccag cacgaactc 
      cacaaagtg cgcgtggaa gccgttgcg ttctccaac 
      aacgacaac tacctcttt tcgatcggc ggccgcgac 
      tgtggctct ttggtggta tgggacgtg acccaaggt 
      caggtgata tgcggttcc gcggtcgca cacggaatc 
      caaggagag gccacggcg atccttagc atgaacagg 
      cgggggccg tgttttatg acagctgga gacaatcac 
      ttggccgtc tggacggtg gacaaggaa gccaggaag 
      gtccactcg ctggacgtc agcaccggc aaaatcaaa 
      agggccatc ctctgcatg gacgtcaac gagcgtgac 
      gaggtgtgc tactgcggc accgccacc ggagacgtg 
      ctcaagatc aggctgaat taccaccac gaccccgac 
      atcctcatc ccagccaag caccccatg acggtgggc 
      tgcttcacg aggatcagc aagaagaag ttgccggcg 
      ggaaaggtg gatttgtac agtttgggg attaagtcg 
      ataaggagg ctgtttgcg gggccgctg ctgattggg 
      gctggtgat gggacaatc gagctggtg gaggagacc 
      aagtgtaaa gggggtggg gataaggat tcaagggtg 
      aaggtgccg tctgtgccg actttgcgt tgtttgaaa 
      agtacccac gtttattca gctgttacg tctttgcag 
      cttatgaaa aaagataca gttatactg gcaggaact 
      atcaattgt gagatttac caaattgat atggcgact 
      tttgataca gtgttgttt gttacttgc catacatcg 
      actatctat gatgttgcc tttccttac aacttttct 
      gatgtattt gccacagct agcaaaaac gacgtccgt 
      gtttggtcg atcaaaact caccaagaa ctccttcgc 
      atccaagta ccaaatttt agtgcctct agcgtctta 
      ttctcacac gatggtaaa tcgatcttg acggcttgg 
      aatgatgga ataatccga tctttcact cctctaacc 
      ggaagactg atttacgcc attctgaac gcccacaat 
      aaaggggtt tcagctttg gcaatcaca catgaaggc 
      gatgtttta gttagcgga ggttgcgaa ggacaagtc 
      agaatgtgg tcagttacg ccttatcgc caacagttg 
      atttgtact ttgaaagaa cacaaagct ccagtttct 
      gccgtcgat attaacaaa gctggtacc gaagcagtc 
      agtgccagt actgatggt acttgtatt atttgggat 
      ttggtacgc caatgtcgt cggcatatt atgtttgga 
      accacgttg tttatgtgc gctcggtac tatcccacg 
      gatgtgcaa gttttgacg ggtggttcc gaccggaaa 
      ctctcatac tgggaggtg cttgatggt actcttgtg 
      cgcgaagtg gagggttcc gagtctggt gccatcaac 
      gccctggac atatcacta gatggcaac atttttgtg 
      actggagga aacgaccaa atcgtcaaa ctttggaat 
      taccaactt ggtattgct actcatatt ggaattggc 
      cattctgct gtcattaca gcagctcgg ttcagtcca 
      gaccagaaa tatattgtg acgaccagt ggttctggc 
      agtatcttt atatggaag tgcccacaa gatttgatt 
      caaccttcc aaagtcaaa attgcagaa gaagccgaa 
      ccaccgaaa gaacaaaag agtgaaacg aaagatgaa 
      gtcatacaa gatctacca acagcaaga agtgatcat 
      agtaaggaa ggtgatcaa aagtgtgtt tgtcgatgt 
      ccttgcgtg aagaagcct gtttgtgag gaaaatggc 
      ggaatggga gatgggatc aagagtcct gaagctaaa 
      gcaagtcct aagaatgac acaagtgat aagaagaat 
      ggatcagtg aacgcaagt cccaaaagt gtctcctcc 
      agtggaaaa aagaatgga tcagttaca ggctcaggg 
      aggaagaac tga
    mRNA sequence
    • >TC003718 RA
      tcacatttc aaacgcatt cagtcaaaa ctgatcaaa 
      actaagcag ttccttcca taatgtctg atgaagaac 
      ttgaagggg atgttggga gtcttgaaa ttaaaggaa 
      ttattggat ttgatggaa ctacaatta agggattta 
      tcatccatc ctgatgaaa aacacgtcg tgtatcctt 
      tgggaaata aagtcacca ttcaggatt ggaccacta 
      aagcccaag tttttctca gtggtcata ctaacacta 
      tttctgcca tcgatgttt ccagatccg gaaaatata 
      ttgctagcg gtcaaatta accacattg gattcaaag 
      cccgcgtca taatttggg acttcgaaa gccacacca 
      tcatctccc agcacgaac tccacaaag tgcgcgtgg 
      aagccgttg cgttctcca acaacgaca actacctct 
      tttcgatcg gcggccgcg actgtggct ctttggtgg 
      tatgggacg tgacccaag gtcaggtga tatgcggtt 
      ccgcggtcg cacacggaa tccaaggag aggccacgg 
      cgatcctta gcatgaaca ggcgggggc cgtgtttta 
      tgacagctg gagacaatc acttggccg tctggacgg 
      tggacaagg aagccagga aggtccact cgctggacg 
      tcagcaccg gcaaaatca aaagggcca tcctctgca 
      tggacgtca acgagcgtg acgaggtgt gctactgcg 
      gcaccgcca ccggagacg tgctcaaga tcaggctga 
      attaccacc acgaccccg acatcctca tcccagcca 
      agcacccca tgacggtgg gctgcttca cgaggatca 
      gcaagaaga agttgccgg cgggaaagg tggatttgt 
      acagtttgg ggattaagt cgataagga ggctgtttg 
      cggggccgc tgctgattg gggctggtg atgggacaa 
      tcgagctgg tggaggaga ccaagtgta aagggggtg 
      gggataagg attcaaggg tgaaggtgc cgtctgtgc 
      cgactttgc gttgtttga aaagtaccc acgtttatt 
      cagctgtta cgtctttgc agcttatga aaaaagata 
      cagttatac tggcaggaa ctatcaatt gtgagattt 
      accaaattg atatggcga cttttgata cagtgttgt 
      ttgttactt gccatacat cgactatct atgatgttg 
      cctttcctt acaactttt ctgatgtat ttgccacag 
      ctagcaaaa acgacgtcc gtgtttggt cgatcaaaa 
      ctcaccaag aactccttc gcatccaag taccaaatt 
      ttagtgcct ctagcgtct tattctcac acgatggta 
      aatcgatct tgacggctt ggaatgatg gaataatcc 
      gatctttca ctcctctaa ccggaagac tgatttacg 
      ccattctga acgcccaca ataaagggg tttcagctt 
      tggcaatca cacatgaag gcgatgttt tagttagcg 
      gaggttgcg aaggacaag tcagaatgt ggtcagtta 
      cgccttatc gccaacagt tgatttgta ctttgaaag 
      aacacaaag ctccagttt ctgccgtcg atattaaca 
      aagctggta ccgaagcag tcagtgcca gtactgatg 
      gtacttgta ttatttggg atttggtac gccaatgtc 
      gtcggcata ttatgtttg gaaccacgt tgtttatgt 
      gcgctcggt actatccca cggatgtgc aagttttga 
      cgggtggtt ccgaccgga aactctcat actgggagg 
      tgcttgatg gtactcttg tgcgcgaag tggagggtt 
      ccgagtctg gtgccatca acgccctgg acatatcac 
      tagatggca acatttttg tgactggag gaaacgacc 
      aaatcgtca aactttgga attaccaac ttggtattg 
      ctactcata ttggaattg gccattctg ctgtcatta 
      cagcagctc ggttcagtc cagaccaga aatatattg 
      tgacgacca gtggttctg gcagtatct ttatatgga 
      agtgcccac aagatttga ttcaacctt ccaaagtca 
      aaattgcag aagaagccg aaccaccga aagaacaaa 
      agagtgaaa cgaaagatg aagtcatac aagatctac 
      caacagcaa gaagtgatc atagtaagg aaggtgatc 
      aaaagtgtg tttgtcgat gtccttgcg tgaagaagc 
      ctgtttgtg aggaaaatg gcggaatgg gagatggga 
      tcaagagtc ctgaagcta aagcaagtc ctaagaatg 
      acacaagtg ataagaaga atggatcag tgaacgcaa 
      gtcccaaaa gtgtctcct ccagtggaa aaaagaatg 
      gatcagtta caggctcag ggaggaaga actgaaata 
      ttttttgtt actggacct attgtttat ttttgtatt 
      ttaatatag tttttttcc aaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY