| General information | |||
| TC003718 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 13637264..13648735 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC003718 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03607 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)remarks: later than schedule, hours 48 remarks: later than schedule, hours 48 remarks: later than schedule, hours 48
remarks: later than schedule, hours 48
remarks: later than schedule, hours 48
remarks - cuticles: later than schedule, hours 48
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC003718 RA
MSDEELEGD VGSLEIKGI IGFDGTTIK GFIIHPDEK HVVYPLGNK VTIQDWTTK AQVFLSGHT NTISAIDVS RSGKYIASG QINHIGFKA RVIIWDFES HTIISQHEL HKVRVEAVA FSNNDNYLF SIGGRDCGS LVVWDVTQG QVICGSAVA HGIQGEATA ILSMNRRGP CFMTAGDNH LAVWTVDKE ARKVHSLDV STGKIKRAI LCMDVNERD EVCYCGTAT GDVLKIRLN YHHDPDILI PAKHPMTVG CFTRISKKK LPAGKVDLY SLGIKSIRR LFAGPLLIG AGDGTIELV EETKCKGGG DKDSRVKVP SVPTLRCLK STHVYSAVT SLQLMKKDT VILAGTINC EIYQIDMAT FDTVLFVTC HTSTIYDVA FPYNFSDVF ATASKNDVR VWSIKTHQE LLRIQVPNF SASSVLFSH DGKSILTAW NDGIIRSFT PLTGRLIYA ILNAHNKGV SALAITHEG DVLVSGGCE GQVRMWSVT PYRQQLICT LKEHKAPVS AVDINKAGT EAVSASTDG TCIIWDLVR QCRRHIMFG TTLFMCARY YPTDVQVLT GGSDRKLSY WEVLDGTLV REVEGSESG AINALDISL DGNIFVTGG NDQIVKLWN YQLGIATHI GIGHSAVIT AARFSPDQK YIVTTSGSG SIFIWKCPQ DLIQPSKVK IAEEAEPPK EQKSETKDE VIQDLPTAR SDHSKEGDQ KCVCRCPCV KKPVCEENG GMGDGIKSP EAKASPKND TSDKKNGSV NASPKSVSS SGKKNGSVT GSGRKN
- >TC003718 RA
atgtctgat gaagaactt gaaggggat gttgggagt cttgaaatt aaaggaatt attggattt gatggaact acaattaag ggatttatc atccatcct gatgaaaaa cacgtcgtg tatcctttg ggaaataaa gtcaccatt caggattgg accactaaa gcccaagtt tttctcagt ggtcatact aacactatt tctgccatc gatgtttcc agatccgga aaatatatt gctagcggt caaattaac cacattgga ttcaaagcc cgcgtcata atttgggac ttcgaaagc cacaccatc atctcccag cacgaactc cacaaagtg cgcgtggaa gccgttgcg ttctccaac aacgacaac tacctcttt tcgatcggc ggccgcgac tgtggctct ttggtggta tgggacgtg acccaaggt caggtgata tgcggttcc gcggtcgca cacggaatc caaggagag gccacggcg atccttagc atgaacagg cgggggccg tgttttatg acagctgga gacaatcac ttggccgtc tggacggtg gacaaggaa gccaggaag gtccactcg ctggacgtc agcaccggc aaaatcaaa agggccatc ctctgcatg gacgtcaac gagcgtgac gaggtgtgc tactgcggc accgccacc ggagacgtg ctcaagatc aggctgaat taccaccac gaccccgac atcctcatc ccagccaag caccccatg acggtgggc tgcttcacg aggatcagc aagaagaag ttgccggcg ggaaaggtg gatttgtac agtttgggg attaagtcg ataaggagg ctgtttgcg gggccgctg ctgattggg gctggtgat gggacaatc gagctggtg gaggagacc aagtgtaaa gggggtggg gataaggat tcaagggtg aaggtgccg tctgtgccg actttgcgt tgtttgaaa agtacccac gtttattca gctgttacg tctttgcag cttatgaaa aaagataca gttatactg gcaggaact atcaattgt gagatttac caaattgat atggcgact tttgataca gtgttgttt gttacttgc catacatcg actatctat gatgttgcc tttccttac aacttttct gatgtattt gccacagct agcaaaaac gacgtccgt gtttggtcg atcaaaact caccaagaa ctccttcgc atccaagta ccaaatttt agtgcctct agcgtctta ttctcacac gatggtaaa tcgatcttg acggcttgg aatgatgga ataatccga tctttcact cctctaacc ggaagactg atttacgcc attctgaac gcccacaat aaaggggtt tcagctttg gcaatcaca catgaaggc gatgtttta gttagcgga ggttgcgaa ggacaagtc agaatgtgg tcagttacg ccttatcgc caacagttg atttgtact ttgaaagaa cacaaagct ccagtttct gccgtcgat attaacaaa gctggtacc gaagcagtc agtgccagt actgatggt acttgtatt atttgggat ttggtacgc caatgtcgt cggcatatt atgtttgga accacgttg tttatgtgc gctcggtac tatcccacg gatgtgcaa gttttgacg ggtggttcc gaccggaaa ctctcatac tgggaggtg cttgatggt actcttgtg cgcgaagtg gagggttcc gagtctggt gccatcaac gccctggac atatcacta gatggcaac atttttgtg actggagga aacgaccaa atcgtcaaa ctttggaat taccaactt ggtattgct actcatatt ggaattggc cattctgct gtcattaca gcagctcgg ttcagtcca gaccagaaa tatattgtg acgaccagt ggttctggc agtatcttt atatggaag tgcccacaa gatttgatt caaccttcc aaagtcaaa attgcagaa gaagccgaa ccaccgaaa gaacaaaag agtgaaacg aaagatgaa gtcatacaa gatctacca acagcaaga agtgatcat agtaaggaa ggtgatcaa aagtgtgtt tgtcgatgt ccttgcgtg aagaagcct gtttgtgag gaaaatggc ggaatggga gatgggatc aagagtcct gaagctaaa gcaagtcct aagaatgac acaagtgat aagaagaat ggatcagtg aacgcaagt cccaaaagt gtctcctcc agtggaaaa aagaatgga tcagttaca ggctcaggg aggaagaac tga
- >TC003718 RA
tcacatttc aaacgcatt cagtcaaaa ctgatcaaa actaagcag ttccttcca taatgtctg atgaagaac ttgaagggg atgttggga gtcttgaaa ttaaaggaa ttattggat ttgatggaa ctacaatta agggattta tcatccatc ctgatgaaa aacacgtcg tgtatcctt tgggaaata aagtcacca ttcaggatt ggaccacta aagcccaag tttttctca gtggtcata ctaacacta tttctgcca tcgatgttt ccagatccg gaaaatata ttgctagcg gtcaaatta accacattg gattcaaag cccgcgtca taatttggg acttcgaaa gccacacca tcatctccc agcacgaac tccacaaag tgcgcgtgg aagccgttg cgttctcca acaacgaca actacctct tttcgatcg gcggccgcg actgtggct ctttggtgg tatgggacg tgacccaag gtcaggtga tatgcggtt ccgcggtcg cacacggaa tccaaggag aggccacgg cgatcctta gcatgaaca ggcgggggc cgtgtttta tgacagctg gagacaatc acttggccg tctggacgg tggacaagg aagccagga aggtccact cgctggacg tcagcaccg gcaaaatca aaagggcca tcctctgca tggacgtca acgagcgtg acgaggtgt gctactgcg gcaccgcca ccggagacg tgctcaaga tcaggctga attaccacc acgaccccg acatcctca tcccagcca agcacccca tgacggtgg gctgcttca cgaggatca gcaagaaga agttgccgg cgggaaagg tggatttgt acagtttgg ggattaagt cgataagga ggctgtttg cggggccgc tgctgattg gggctggtg atgggacaa tcgagctgg tggaggaga ccaagtgta aagggggtg gggataagg attcaaggg tgaaggtgc cgtctgtgc cgactttgc gttgtttga aaagtaccc acgtttatt cagctgtta cgtctttgc agcttatga aaaaagata cagttatac tggcaggaa ctatcaatt gtgagattt accaaattg atatggcga cttttgata cagtgttgt ttgttactt gccatacat cgactatct atgatgttg cctttcctt acaactttt ctgatgtat ttgccacag ctagcaaaa acgacgtcc gtgtttggt cgatcaaaa ctcaccaag aactccttc gcatccaag taccaaatt ttagtgcct ctagcgtct tattctcac acgatggta aatcgatct tgacggctt ggaatgatg gaataatcc gatctttca ctcctctaa ccggaagac tgatttacg ccattctga acgcccaca ataaagggg tttcagctt tggcaatca cacatgaag gcgatgttt tagttagcg gaggttgcg aaggacaag tcagaatgt ggtcagtta cgccttatc gccaacagt tgatttgta ctttgaaag aacacaaag ctccagttt ctgccgtcg atattaaca aagctggta ccgaagcag tcagtgcca gtactgatg gtacttgta ttatttggg atttggtac gccaatgtc gtcggcata ttatgtttg gaaccacgt tgtttatgt gcgctcggt actatccca cggatgtgc aagttttga cgggtggtt ccgaccgga aactctcat actgggagg tgcttgatg gtactcttg tgcgcgaag tggagggtt ccgagtctg gtgccatca acgccctgg acatatcac tagatggca acatttttg tgactggag gaaacgacc aaatcgtca aactttgga attaccaac ttggtattg ctactcata ttggaattg gccattctg ctgtcatta cagcagctc ggttcagtc cagaccaga aatatattg tgacgacca gtggttctg gcagtatct ttatatgga agtgcccac aagatttga ttcaacctt ccaaagtca aaattgcag aagaagccg aaccaccga aagaacaaa agagtgaaa cgaaagatg aagtcatac aagatctac caacagcaa gaagtgatc atagtaagg aaggtgatc aaaagtgtg tttgtcgat gtccttgcg tgaagaagc ctgtttgtg aggaaaatg gcggaatgg gagatggga tcaagagtc ctgaagcta aagcaagtc ctaagaatg acacaagtg ataagaaga atggatcag tgaacgcaa gtcccaaaa gtgtctcct ccagtggaa aaaagaatg gatcagtta caggctcag ggaggaaga actgaaata ttttttgtt actggacct attgtttat ttttgtatt ttaatatag tttttttcc aaaa
