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Gene information
General information
TC003718 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 13637264..13648735
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003718
    iBeetle screen iB_03607

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    remarks: later than schedule, hours 48 remarks: later than schedule, hours 48 remarks: later than schedule, hours 48
    Pupal morphology
    remarks: later than schedule, hours 48
    Adult morphology
    remarks: later than schedule, hours 48 remarks: later than schedule, hours 48
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 remarks: later than schedule, hours 48 (egglay)
    remarks: later than schedule, hours 48
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     remarks: later than schedule, hours 48
    remarks - cuticles: later than schedule, hours 48
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003718 RA
      MSDEELEGD VGSLEIKGI IGFDGTTIK GFIIHPDEK 
HVVYPLGNK VTIQDWTTK AQVFLSGHT NTISAIDVS 
RSGKYIASG QINHIGFKA RVIIWDFES HTIISQHEL 
HKVRVEAVA FSNNDNYLF SIGGRDCGS LVVWDVTQG 
QVICGSAVA HGIQGEATA ILSMNRRGP CFMTAGDNH 
LAVWTVDKE ARKVHSLDV STGKIKRAI LCMDVNERD 
EVCYCGTAT GDVLKIRLN YHHDPDILI PAKHPMTVG 
CFTRISKKK LPAGKVDLY SLGIKSIRR LFAGPLLIG 
AGDGTIELV EETKCKGGG DKDSRVKVP SVPTLRCLK 
STHVYSAVT SLQLMKKDT VILAGTINC EIYQIDMAT 
FDTVLFVTC HTSTIYDVA FPYNFSDVF ATASKNDVR 
VWSIKTHQE LLRIQVPNF SASSVLFSH DGKSILTAW 
NDGIIRSFT PLTGRLIYA ILNAHNKGV SALAITHEG 
DVLVSGGCE GQVRMWSVT PYRQQLICT LKEHKAPVS 
AVDINKAGT EAVSASTDG TCIIWDLVR QCRRHIMFG 
TTLFMCARY YPTDVQVLT GGSDRKLSY WEVLDGTLV 
REVEGSESG AINALDISL DGNIFVTGG NDQIVKLWN 
YQLGIATHI GIGHSAVIT AARFSPDQK YIVTTSGSG 
SIFIWKCPQ DLIQPSKVK IAEEAEPPK EQKSETKDE 
VIQDLPTAR SDHSKEGDQ KCVCRCPCV KKPVCEENG 
GMGDGIKSP EAKASPKND TSDKKNGSV NASPKSVSS 
SGKKNGSVT GSGRKN
    CDS sequence
    • >TC003718 RA
      atgtctgat gaagaactt gaaggggat gttgggagt 
cttgaaatt aaaggaatt attggattt gatggaact 
acaattaag ggatttatc atccatcct gatgaaaaa 
cacgtcgtg tatcctttg ggaaataaa gtcaccatt 
caggattgg accactaaa gcccaagtt tttctcagt 
ggtcatact aacactatt tctgccatc gatgtttcc 
agatccgga aaatatatt gctagcggt caaattaac 
cacattgga ttcaaagcc cgcgtcata atttgggac 
ttcgaaagc cacaccatc atctcccag cacgaactc 
cacaaagtg cgcgtggaa gccgttgcg ttctccaac 
aacgacaac tacctcttt tcgatcggc ggccgcgac 
tgtggctct ttggtggta tgggacgtg acccaaggt 
caggtgata tgcggttcc gcggtcgca cacggaatc 
caaggagag gccacggcg atccttagc atgaacagg 
cgggggccg tgttttatg acagctgga gacaatcac 
ttggccgtc tggacggtg gacaaggaa gccaggaag 
gtccactcg ctggacgtc agcaccggc aaaatcaaa 
agggccatc ctctgcatg gacgtcaac gagcgtgac 
gaggtgtgc tactgcggc accgccacc ggagacgtg 
ctcaagatc aggctgaat taccaccac gaccccgac 
atcctcatc ccagccaag caccccatg acggtgggc 
tgcttcacg aggatcagc aagaagaag ttgccggcg 
ggaaaggtg gatttgtac agtttgggg attaagtcg 
ataaggagg ctgtttgcg gggccgctg ctgattggg 
gctggtgat gggacaatc gagctggtg gaggagacc 
aagtgtaaa gggggtggg gataaggat tcaagggtg 
aaggtgccg tctgtgccg actttgcgt tgtttgaaa 
agtacccac gtttattca gctgttacg tctttgcag 
cttatgaaa aaagataca gttatactg gcaggaact 
atcaattgt gagatttac caaattgat atggcgact 
tttgataca gtgttgttt gttacttgc catacatcg 
actatctat gatgttgcc tttccttac aacttttct 
gatgtattt gccacagct agcaaaaac gacgtccgt 
gtttggtcg atcaaaact caccaagaa ctccttcgc 
atccaagta ccaaatttt agtgcctct agcgtctta 
ttctcacac gatggtaaa tcgatcttg acggcttgg 
aatgatgga ataatccga tctttcact cctctaacc 
ggaagactg atttacgcc attctgaac gcccacaat 
aaaggggtt tcagctttg gcaatcaca catgaaggc 
gatgtttta gttagcgga ggttgcgaa ggacaagtc 
agaatgtgg tcagttacg ccttatcgc caacagttg 
atttgtact ttgaaagaa cacaaagct ccagtttct 
gccgtcgat attaacaaa gctggtacc gaagcagtc 
agtgccagt actgatggt acttgtatt atttgggat 
ttggtacgc caatgtcgt cggcatatt atgtttgga 
accacgttg tttatgtgc gctcggtac tatcccacg 
gatgtgcaa gttttgacg ggtggttcc gaccggaaa 
ctctcatac tgggaggtg cttgatggt actcttgtg 
cgcgaagtg gagggttcc gagtctggt gccatcaac 
gccctggac atatcacta gatggcaac atttttgtg 
actggagga aacgaccaa atcgtcaaa ctttggaat 
taccaactt ggtattgct actcatatt ggaattggc 
cattctgct gtcattaca gcagctcgg ttcagtcca 
gaccagaaa tatattgtg acgaccagt ggttctggc 
agtatcttt atatggaag tgcccacaa gatttgatt 
caaccttcc aaagtcaaa attgcagaa gaagccgaa 
ccaccgaaa gaacaaaag agtgaaacg aaagatgaa 
gtcatacaa gatctacca acagcaaga agtgatcat 
agtaaggaa ggtgatcaa aagtgtgtt tgtcgatgt 
ccttgcgtg aagaagcct gtttgtgag gaaaatggc 
ggaatggga gatgggatc aagagtcct gaagctaaa 
gcaagtcct aagaatgac acaagtgat aagaagaat 
ggatcagtg aacgcaagt cccaaaagt gtctcctcc 
agtggaaaa aagaatgga tcagttaca ggctcaggg 
aggaagaac tga
    mRNA sequence
    • >TC003718 RA
      tcacatttc aaacgcatt cagtcaaaa ctgatcaaa 
actaagcag ttccttcca taatgtctg atgaagaac 
ttgaagggg atgttggga gtcttgaaa ttaaaggaa 
ttattggat ttgatggaa ctacaatta agggattta 
tcatccatc ctgatgaaa aacacgtcg tgtatcctt 
tgggaaata aagtcacca ttcaggatt ggaccacta 
aagcccaag tttttctca gtggtcata ctaacacta 
tttctgcca tcgatgttt ccagatccg gaaaatata 
ttgctagcg gtcaaatta accacattg gattcaaag 
cccgcgtca taatttggg acttcgaaa gccacacca 
tcatctccc agcacgaac tccacaaag tgcgcgtgg 
aagccgttg cgttctcca acaacgaca actacctct 
tttcgatcg gcggccgcg actgtggct ctttggtgg 
tatgggacg tgacccaag gtcaggtga tatgcggtt 
ccgcggtcg cacacggaa tccaaggag aggccacgg 
cgatcctta gcatgaaca ggcgggggc cgtgtttta 
tgacagctg gagacaatc acttggccg tctggacgg 
tggacaagg aagccagga aggtccact cgctggacg 
tcagcaccg gcaaaatca aaagggcca tcctctgca 
tggacgtca acgagcgtg acgaggtgt gctactgcg 
gcaccgcca ccggagacg tgctcaaga tcaggctga 
attaccacc acgaccccg acatcctca tcccagcca 
agcacccca tgacggtgg gctgcttca cgaggatca 
gcaagaaga agttgccgg cgggaaagg tggatttgt 
acagtttgg ggattaagt cgataagga ggctgtttg 
cggggccgc tgctgattg gggctggtg atgggacaa 
tcgagctgg tggaggaga ccaagtgta aagggggtg 
gggataagg attcaaggg tgaaggtgc cgtctgtgc 
cgactttgc gttgtttga aaagtaccc acgtttatt 
cagctgtta cgtctttgc agcttatga aaaaagata 
cagttatac tggcaggaa ctatcaatt gtgagattt 
accaaattg atatggcga cttttgata cagtgttgt 
ttgttactt gccatacat cgactatct atgatgttg 
cctttcctt acaactttt ctgatgtat ttgccacag 
ctagcaaaa acgacgtcc gtgtttggt cgatcaaaa 
ctcaccaag aactccttc gcatccaag taccaaatt 
ttagtgcct ctagcgtct tattctcac acgatggta 
aatcgatct tgacggctt ggaatgatg gaataatcc 
gatctttca ctcctctaa ccggaagac tgatttacg 
ccattctga acgcccaca ataaagggg tttcagctt 
tggcaatca cacatgaag gcgatgttt tagttagcg 
gaggttgcg aaggacaag tcagaatgt ggtcagtta 
cgccttatc gccaacagt tgatttgta ctttgaaag 
aacacaaag ctccagttt ctgccgtcg atattaaca 
aagctggta ccgaagcag tcagtgcca gtactgatg 
gtacttgta ttatttggg atttggtac gccaatgtc 
gtcggcata ttatgtttg gaaccacgt tgtttatgt 
gcgctcggt actatccca cggatgtgc aagttttga 
cgggtggtt ccgaccgga aactctcat actgggagg 
tgcttgatg gtactcttg tgcgcgaag tggagggtt 
ccgagtctg gtgccatca acgccctgg acatatcac 
tagatggca acatttttg tgactggag gaaacgacc 
aaatcgtca aactttgga attaccaac ttggtattg 
ctactcata ttggaattg gccattctg ctgtcatta 
cagcagctc ggttcagtc cagaccaga aatatattg 
tgacgacca gtggttctg gcagtatct ttatatgga 
agtgcccac aagatttga ttcaacctt ccaaagtca 
aaattgcag aagaagccg aaccaccga aagaacaaa 
agagtgaaa cgaaagatg aagtcatac aagatctac 
caacagcaa gaagtgatc atagtaagg aaggtgatc 
aaaagtgtg tttgtcgat gtccttgcg tgaagaagc 
ctgtttgtg aggaaaatg gcggaatgg gagatggga 
tcaagagtc ctgaagcta aagcaagtc ctaagaatg 
acacaagtg ataagaaga atggatcag tgaacgcaa 
gtcccaaaa gtgtctcct ccagtggaa aaaagaatg 
gatcagtta caggctcag ggaggaaga actgaaata 
ttttttgtt actggacct attgtttat ttttgtatt 
ttaatatag tttttttcc aaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY