Status
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Gene information
General information
TC003716 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 13610124..13616518
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003716
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03606

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 1

    elytra size shortened - Number of animals showing the phenotype: 1

    prothoracic leg partially not present - Number of animals showing the phenotype: 1

    elytra dorsal not closed - Number of animals showing the phenotype: 1
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003716 RA
      MEGLQESEM LQSPNEKSE MYSTLSQSH LIDASDVTK 
      RRSSNRSIK RKKFDDELV EYSLGLPGI NTVKSNRAR 
      TQSHSDFSV ASPSTPPIV TTPTVSHTP ELKRKPASK 
      TVTSSSRRN KKGRQQNQF ATKDLGRWK PTDDLALII 
      GVQQTNDLR TVHLGTKFS CKFTVQELQ QRWYALLYD 
      QAVSRVAVS AMRNLHPDM IAAVQDKAL WSQAEEQIL 
      STVKSSANP TIDVFAELL AQNPDVFYP GRTASALFR 
      HWQTLKMYH LLPDQILGP LPTSGRPIM TFNDAEDII 
      QDSELSEPP DEALDRELR LQQRKNIKQ IRQLENEVG 
      RWNVLVDSV TGICPGELD GQTLAVLRG RMVRYLMRS 
      KEIIIGRCA KGYNVDIDL SLEGPAHKV SRRQGTLRL 
      RNTGEFYLS SEGRRPIFV DGRPITAGN KVRLFDNAV 
      VEISCLRFI FSVNHDLIR TIHDSLRYN GPA
    CDS sequence
    • >TC003716 RA
      atggagggt ttacaagaa tctgaaatg ttacaatca 
      cccaacgaa aagtcggaa atgtacagc acattgtct 
      caaagccat ttaattgat gcttcagac gtcacaaaa 
      cgtcgaagt tcaaatcgc tcgatcaaa cgcaagaaa 
      tttgacgac gaattggtg gagtacagt ctaggcttg 
      cccggcatt aatacagta aagagtaac agagcgcgt 
      acacagtcc cattccgac ttttctgtg gctagtcct 
      tcgactcct ccgatcgtt acaactcca actgtaagt 
      cacacgcct gagttaaag cgaaagccg gcgtctaag 
      actgtaacg agcagttcg cggcgtaac aaaaagggg 
      cgccaacag aatcagttt gcaactaaa gatttgggc 
      aggtggaag cctactgac gatcttgcg ttaattatt 
      ggggttcag caaaccaac gatttgcgg acggtgcat 
      ttgggtaca aagttttcc tgcaaattt actgtgcaa 
      gaactacaa cagcgttgg tatgctttg ttatatgac 
      caagctgtg tcccgcgtg gctgtgtct gctatgagg 
      aacttgcac cctgatatg atcgcagca gtgcaggat 
      aaggcgctt tggagtcaa gccgaagag cagatttta 
      agcaccgta aaatcaagc gccaacccc acaattgat 
      gtatttgcg gagttgctg gcacaaaat cctgatgtt 
      ttttatcct ggaagaacg gcaagtgcg ttgtttagg 
      cactggcaa actttgaaa atgtaccat ttgttgcct 
      gatcaaatt ttggggcct ctgccaacg tcgggacgt 
      cccataatg acttttaac gatgctgaa gacattatt 
      caagattca gagttgagt gaacctccg gatgaggca 
      ctcgatcgg gagttgaga ttacaacag aggaaaaat 
      attaaacaa atcagacag ttggagaat gaagttggt 
      agatggaat gtgctggtt gatagtgtt actggaatc 
      tgtcctggg gagttagat ggacaaact ctagctgtg 
      ctgagaggt agaatggtg aggtactta atgagaagc 
      aaagaaatt atcattgga agatgtgcc aaggggtat 
      aatgttgat attgatctc agtttggag ggacctgca 
      cataaggtt tcaagacgt cagggaact ctaagatta 
      agaaacacg ggtgaattc tacttgtct tctgaaggc 
      cgccgtcca attttcgta gatggacgt ccaataaca 
      gccggaaac aaagtcaga ttgtttgac aatgcagta 
      gttgaaatt tcttgcctt aggttcatt ttttctgta 
      aaccacgac ttgattaga actattcac gattcacta 
      aggtataat ggaccagct taa
    mRNA sequence
    • >TC003716 RA
      acgtaaatc tcgttattt ggaagtgtt tatgttttt 
      gctcgaaaa agcctaaat ggagggttt acaagaatc 
      tgaaatgtt acaatcacc caacgaaaa gtcggaaat 
      gtacagcac attgtctca aagccattt aattgatgc 
      ttcagacgt cacaaaacg tcgaagttc aaatcgctc 
      gatcaaacg caagaaatt tgacgacga attggtgga 
      gtacagtct aggcttgcc cggcattaa tacagtaaa 
      gagtaacag agcgcgtac acagtccca ttccgactt 
      ttctgtggc tagtccttc gactcctcc gatcgttac 
      aactccaac tgtaagtca cacgcctga gttaaagcg 
      aaagccggc gtctaagac tgtaacgag cagttcgcg 
      gcgtaacaa aaaggggcg ccaacagaa tcagtttgc 
      aactaaaga tttgggcag gtggaagcc tactgacga 
      tcttgcgtt aattattgg ggttcagca aaccaacga 
      tttgcggac ggtgcattt gggtacaaa gttttcctg 
      caaatttac tgtgcaaga actacaaca gcgttggta 
      tgctttgtt atatgacca agctgtgtc ccgcgtggc 
      tgtgtctgc tatgaggaa cttgcaccc tgatatgat 
      cgcagcagt gcaggataa ggcgctttg gagtcaagc 
      cgaagagca gattttaag caccgtaaa atcaagcgc 
      caaccccac aattgatgt atttgcgga gttgctggc 
      acaaaatcc tgatgtttt ttatcctgg aagaacggc 
      aagtgcgtt gtttaggca ctggcaaac tttgaaaat 
      gtaccattt gttgcctga tcaaatttt ggggcctct 
      gccaacgtc gggacgtcc cataatgac ttttaacga 
      tgctgaaga cattattca agattcaga gttgagtga 
      acctccgga tgaggcact cgatcggga gttgagatt 
      acaacagag gaaaaatat taaacaaat cagacagtt 
      ggagaatga agttggtag atggaatgt gctggttga 
      tagtgttac tggaatctg tcctgggga gttagatgg 
      acaaactct agctgtgct gagaggtag aatggtgag 
      gtacttaat gagaagcaa agaaattat cattggaag 
      atgtgccaa ggggtataa tgttgatat tgatctcag 
      tttggaggg acctgcaca taaggtttc aagacgtca 
      gggaactct aagattaag aaacacggg tgaattcta 
      cttgtcttc tgaaggccg ccgtccaat tttcgtaga 
      tggacgtcc aataacagc cggaaacaa agtcagatt 
      gtttgacaa tgcagtagt tgaaatttc ttgccttag 
      gttcatttt ttctgtaaa ccacgactt gattagaac 
      tattcacga ttcactaag gtataatgg accagctta 
      atttaaaca aagttttgt aaaaaatgt att
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY