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Gene information
General information
TC003707 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 13260864..13279258
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003707
    iBeetle screen iB_03604

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003707 RA
      MKYPNVSGG TNMAVSGNG SHVDEVDSL FSSTCWQNS 
      NINLQNDYS LTLDSSMSN LGADKLLLD SPISQDDNE 
      IETDALDKL FLSSQPTNS SLAELKPLP PFTGYTANL 
      SINGIQGHH YHTISQRIP EENNNTYNS YNEQNIVSS 
      STCNVSADS AGDCAKTMY SVDEVVKLG YSDCANPDS 
      VSSAKMYED CSHPSADSV THIKTEIAE YDIAIDFAN 
      IIGSAMADT TVPNTNPDG DDAAGSRDS WMDIDAFIG 
      ETCGEQKNL IITQDGLSE FVIPSTSSE YTKPQQIQP 
      QQGSSTLQT LLTHGYMPL LQNRLQNGP PIKQEAPSS 
      TNFGSDLIT TSSPPANVV STTDNLLLT VNGRFMPQY 
      GVHSLDQDH RIRSPDLLG QNYPHTTTT NTPNAPKKS 
      RSRAQKKQA QQATSTVFQ NEQNLGLLG KEKPVHRCT 
      ICNRGFLNK SNIKVHLRT HTGEKPFRC DTCAKAFRQ 
      KAHLLKHQQ IHKRIGRD
    CDS sequence
    • >TC003707 RA
      atgaaatat cccaacgta tctggtggc accaacatg 
      gcagtcagc ggcaatggc tctcacgtt gacgaagtc 
      gattcgctc ttcagctcc acgtgttgg caaaactcc 
      aacatcaac cttcaaaac gactattca ttaacccta 
      gacagctca atgagcaac ctaggggcg gacaagctc 
      ctcctggac tccccgatc agtcaagac gacaacgag 
      atcgaaacg gacgcttta gacaaactg tttttaagt 
      tcacaacct actaactcc agtttagcc gaactgaag 
      cctctgccc cctttcacc ggctacacc gccaacctc 
      tccatcaac gggatccag ggccaccac taccacacg 
      atatcccag cggatcccc gaggagaac aacaacacc 
      tacaacagt tacaacgaa cagaacatt gtctcgagt 
      tctacttgt aatgtgagt gcggactct gcgggtgac 
      tgtgcgaaa acaatgtat tccgtcgac gaagtggtc 
      aagttaggt tactcagac tgtgctaac cctgactca 
      gtgtctagt gcgaaaatg tacgaggac tgcagccac 
      ccctccgcc gactcagtg acgcacata aagacggaa 
      atcgccgaa tacgacatt gcgatagac ttcgccaat 
      atcataggc tcggcgatg gccgacacc accgtcccc 
      aacaccaac cccgacgga gacgacgcc gccggctcc 
      agggacagc tggatggac atcgacgcc ttcatcggg 
      gagacctgc ggcgagcaa aagaacctc atcatcacc 
      caggacggc ctctctgag tttgtgata ccttcgacg 
      agttcagag tacaccaag ccccagcag atccagccc 
      cagcagggc agctccacc ctgcaaacg ctcctcaca 
      cacggatac atgcccctg ctccagaac cggctccag 
      aacggcccc cctatcaaa caagaggcc cccagctcg 
      accaacttt ggcagcgac ctgatcacg acgtccagt 
      ccgccagcg aacgtcgtc tccaccacc gacaacctc 
      ctgttgacg gtcaacggc cgcttcatg ccccagtac 
      ggggtgcac agcctggac caggaccac agaatcagg 
      agtcccgac ctcctcggc cagaactac ccccacacc 
      accaccacc aacaccccc aacgccccc aagaagtcg 
      aggagtcgg gcgcagaaa aaacaagcg cagcaagcc 
      acctcgacg gtgttccaa aacgaacag aatttaggt 
      ttgttgggc aaagagaag ccggttcat agatgtact 
      atttgtaat cggggcttc ctcaacaag tccaatatt 
      aaggtgcat ttgaggact cacacgggg gagaaacct 
      tttagatgc gatacttgc gccaaggcc tttcggcaa 
      aaagcacac ttgctgaaa catcagcag atacacaag 
      aggatcgga cgcgattga
    mRNA sequence
    • >TC003707 RA
      agacatcaa aatctgtag tcatcatct agacagagc 
      ctccccacc aatccaaag taggttacc tcagtgtac 
      tcagtatgg atctacaac aggtacagt tatattctt 
      gtagagggg acttctagg ggccagtgg tattgagta 
      gggcttcca ctgtccagt agtgcgtcc tctgtcgca 
      aagagcacg tccgtgttt acgaaattt cggcaagtt 
      gcgacccca aaatttgca tacccgacg agcctccac 
      accctcccg ggtccgtgt cgtggtgat ttgagtgcc 
      gccgagggt gtttccggg atcttgtgc cggcggaat 
      gacagtgca ttgatgggt tgacccgga gcatccaag 
      actgctcga ggacttaaa ccgatgaca ccgtaacac 
      acttataaa taattattc gtgttacaa aagaaaacg 
      ctgccagtg ctcgaattc gggatgtgt ttgcggagg 
      aaaagcaag attttgcac aaacccaag cggtgatcg 
      aaaaaacaa tagactcaa aaacggatg tgcagtgtg 
      ttattccca cttgttgcg gatatactt cagtgaccg 
      atttttgtt ttactaata tcggaattt acccctttt 
      tgtccggcc tcagcgatg cggacgccc ttattcccg 
      tgccgcgtg aactaaggt cgcgcggtg gccccagcc 
      ctcagcctc cgtccccaa atggcaaca atgaaatat 
      cccaacgta tctggtggc accaacatg gcagtcagc 
      ggcaatggc tctcacgtt gacgaagtc gattcgctc 
      ttcagctcc acgtgttgg caaaactcc aacatcaac 
      cttcaaaac gactattca ttaacccta gacagctca 
      atgagcaac ctaggggcg gacaagctc ctcctggac 
      tccccgatc agtcaagac gacaacgag atcgaaacg 
      gacgcttta gacaaactg tttttaagt tcacaacct 
      actaactcc agtttagcc gaactgaag cctctgccc 
      cctttcacc ggctacacc gccaacctc tccatcaac 
      gggatccag ggccaccac taccacacg atatcccag 
      cggatcccc gaggagaac aacaacacc tacaacagt 
      tacaacgaa cagaacatt gtctcgagt tctacttgt 
      aatgtgagt gcggactct gcgggtgac tgtgcgaaa 
      acaatgtat tccgtcgac gaagtggtc aagttaggt 
      tactcagac tgtgctaac cctgactca gtgtctagt 
      gcgaaaatg tacgaggac tgcagccac ccctccgcc 
      gactcagtg acgcacata aagacggaa atcgccgaa 
      tacgacatt gcgatagac ttcgccaat atcataggc 
      tcggcgatg gccgacacc accgtcccc aacaccaac 
      cccgacgga gacgacgcc gccggctcc agggacagc 
      tggatggac atcgacgcc ttcatcggg gagacctgc 
      ggcgagcaa aagaacctc atcatcacc caggacggc 
      ctctctgag tttgtgata ccttcgacg agttcagag 
      tacaccaag ccccagcag atccagccc cagcagggc 
      agctccacc ctgcaaacg ctcctcaca cacggatac 
      atgcccctg ctccagaac cggctccag aacggcccc 
      cctatcaaa caagaggcc cccagctcg accaacttt 
      ggcagcgac ctgatcacg acgtccagt ccgccagcg 
      aacgtcgtc tccaccacc gacaacctc ctgttgacg 
      gtcaacggc cgcttcatg ccccagtac ggggtgcac 
      agcctggac caggaccac agaatcagg agtcccgac 
      ctcctcggc cagaactac ccccacacc accaccacc 
      aacaccccc aacgccccc aagaagtcg aggagtcgg 
      gcgcagaaa aaacaagcg cagcaagcc acctcgacg 
      gtgttccaa aacgaacag aatttaggt ttgttgggc 
      aaagagaag ccggttcat agatgtact atttgtaat 
      cggggcttc ctcaacaag tccaatatt aaggtgcat 
      ttgaggact cacacgggg gagaaacct tttagatgc 
      gatacttgc gccaaggcc tttcggcaa aaagcacac 
      ttgctgaaa catcagcag atacacaag aggatcgga 
      cgcgattga ttcttgtag ggtttgggc gaaggtaag 
      gagtcttgt ttagcgcat gacatcgtg tcatgatgt 
      cactgagcc ccacaacac ttcttatta ttgcacttc 
      gccaactta ggtcaggcc aatgagcaa aaatttgga 
      ggaaacttc tcaggttct cgtttcttg ccattttgc 
      aacgtttaa agcgattaa ttaagagtt taagtttga 
      acggtactg agaatggaa agcgaaatg acactgaat 
      tttacgaaa aatagcaaa caatacaat aattgggtg 
      tcgctccta aattaaacc agttcacac tcagctatt 
      atcccattt taaatattt gttacggac tggtacctt 
      cagtagttt tgtatgaaa tacaaatgc atgaaaaaa 
      atccgtgcc gttttaaaa tgaaataaa caatttcta 
      aacaaatag ataataact gagctatga gctcatttg 
      aattagggg caacttcga attgttggc tatttttcc 
      taaaaattt cacccggcg gccgttccc ggtacagtt 
      cggctttat gaggagatc ccgaaacta cattgcgct 
      gcgtgacac tgttccact tcaccatgc cctctgctg 
      gtacacaat ctaaccttc aagttgtgc tactataat 
      tttatgaaa ttcacattt ttcgttcca tgatttatg 
      ctcgtgaaa gtcatgctt ggtttgttc gttttgtat 
      ttttgctgg ttggcccgt cgattccaa acgtgctat 
      aaaaaaggt acctcatat ttaacaaaa tgtatattc 
      ctattaatg gacaatgtg caatttcat ctagtgagc 
      cggtgcaaa caacaacga atctaagtt ctataggaa 
      tatcacagt tcactcttt ggtggcttt ctttgtcct 
      atttttatt atttatttg tattattac gttttgcgc 
      attgttgtg gtatatgtt tttatttat tttaaactt 
      gtattataa tgtctcgtt ttctgtctt aacccctgc 
      cttctcaca cgttctatt tatttatat taaccttat 
      tttgttgtt ttctttgtt gtatttagt cggttgatg 
      taaaattcg ttcaagatt tctcaggtc ctcttttta 
      atgcacttc ttgtgataa aaaataaaa tacttaata 
      tttaagtaa taattaagt atttgtatt tttacaaaa 
      tatttaatt gtatcgtgt aaatctttg attgattgt 
      acctaggaa agttttaat taaggcaga attcacaac 
      gaatcctat tgtaaattg tgtaattat gtgtagtcc 
      ccttttcct atttattac tcgttgctt ctgcccttt 
      cgtaagaac aaagcgtct tcctggacc aacgtataa 
      tagatctca gtttttcta acacaaaag ttgctttgc 
      acctagcaa ttgtgattt cagttttgt cacatgttg 
      tcgctacgt acgaaaact ccccgacgt agtactgaa 
      acgtaagtt ttattaaac atagtttga ttatgtgat 
      aatcagaaa ataagtaaa aatacttgt aatttataa 
      ataacacat agcttgacg acaaataaa tttttgtat 
      gtatctagg ataagtgca gtaaacaaa tagttttag 
      gtagcccct tagacactt aaattaatt attgttatt 
      attttattg ttgattata ttaataagt atgttgtat 
      tatgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY