Status
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Gene information
General information
TC003702 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 12931086..12935683
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003702
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03603

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003702 RA
      MSGDLNQEI IAYHIEETK INTEVDATS AEPIPDRST 
      LEDILKSSR EQRTSDEKN EGSAQSDKS KSVKYTPSD 
      EKLELQSVN IHDLAVLMK RETDELAVE DIFSLRSED 
      KSIDTVSVS YLKPPARYP DSVQDQEFE VDNESVYSF 
      LMKRESEET LTTPAQSPE KSSESIPKD EKQIDRNMY 
      YELHRLYTA NYNTQKWKN NYLQKKLAQ YYNRRKMYH 
      VFVVDKSQQ EYAKMYEKL LESFAVKQD EVAREQAAL 
      ENEISVLRN KHRAKSEEV ANLLVELQQ AEADVSKQF 
      SYTVLNAAK MVERFLKRQ RMQLTRFKE MHLAYIKLR 
      NKNNEAQDT LDSLDNLGP NCRLIDYEQ LKTDNRNLY 
      EKLEEKELY LNSLRQECR NVMQILAHT REKTNVVNL 
      DIMKYQEQL DLVLADGME AREQLSMMK HERDYIRRQ 
      INKLKLETG LLGRPKLLR DLERSMEEV EEGLDQLEE 
      YKEACEVAS ETMVAVRKY ISRLSETGE SGKITKRIQ 
      F
    CDS sequence
    • >TC003702 RA
      atgtctgga gatttaaat caagaaatt attgcatat 
      catatagag gaaactaaa atcaacact gaagtggat 
      gcaacttct gccgaaccc attcctgat agatccact 
      ttggaagac atattaaaa agtagtagg gaacagaga 
      accagcgat gaaaaaaac gaaggttca gctcaaagt 
      gacaagagc aaaagtgtt aaatataca ccaagcgat 
      gaaaaactg gaactacaa agtgtaaat atccatgac 
      ttggctgta cttatgaaa cgcgagact gacgaactg 
      gccgttgaa gatattttt tctttacga agcgaagac 
      aaatctata gatacagtc agtgtttct tacttgaaa 
      ccacctgct cgctaccct gactctgtt caggaccag 
      gaattcgaa gtagacaat gagtctgtt tattccttt 
      cttatgaaa cgagaatcc gaagaaact ctaacaaca 
      ccggcacaa agtcccgaa aaatcatcg gaatctatc 
      cccaaagac gagaaacaa atcgacagg aacatgtac 
      tacgaactg cacaggtta tacactgcg aactacaac 
      acccagaag tggaagaac aattacctt cagaagaag 
      ttagcacag tactataac cgccgaaag atgtatcat 
      gtgtttgtc gtggacaag tcccaacag gagtacgcc 
      aagatgtat gaaaaactt ctagaatcg tttgcagtc 
      aaacaggac gaagttgca agagaacaa gcagcgttg 
      gagaacgaa ataagcgtt ctgcgcaac aaacatcgc 
      gccaaaagt gaagaagtt gccaacctc ctggtggaa 
      ctccagcaa gccgaagca gacgtgagc aagcagttc 
      agctacacg gttctaaat gctgcgaaa atggtcgaa 
      cgattcctc aaacgccag cggatgcag ctgacacgt 
      ttcaaggaa atgcatctt gcttacata aaattgcga 
      aacaagaac aacgaagcg caagatact ttggattcg 
      cttgataac ttgggcccc aactgccgc ctaatcgac 
      tacgaacag ctgaaaacg gataacagg aacctttac 
      gaaaagctg gaggagaag gaattgtac ttaaactcg 
      ttaagacag gagtgtcga aacgtgatg cagatcttg 
      gctcatacg agggaaaag acgaatgtt gtgaatttg 
      gacattatg aagtatcag gagcagctc gatttggtg 
      ttagcagat ggcatggaa gcacgggaa cagttgagt 
      atgatgaag catgagagg gattacatc aggcggcag 
      ataaataaa ctaaagctt gagactggt ttgcttgga 
      cgaccaaaa ctgttaagg gatttggaa aggtcgatg 
      gaagaagtg gaggaaggg ttggatcaa ctagaggag 
      tataaagaa gcttgtgag gtggcatct gaaaccatg 
      gtagctgtg aggaaatat attagcaga ttgagcgag 
      acaggggag tcaggaaag attacgaaa agaattcag 
      ttctga
    mRNA sequence
    • >TC003702 RA
      tcaattttt tatgtaatt ttttacgcg atttatagt 
      tttttgtga tgtctggag atttaaatc aagaaatta 
      ttgcatatc atatagagg aaactaaaa tcaacactg 
      aagtggatg caacttctg ccgaaccca ttcctgata 
      gatccactt tggaagaca tattaaaaa gtagtaggg 
      aacagagaa ccagcgatg aaaaaaacg aaggttcag 
      ctcaaagtg acaagagca aaagtgtta aatatacac 
      caagcgatg aaaaactgg aactacaaa gtgtaaata 
      tccatgact tggctgtac ttatgaaac gcgagactg 
      acgaactgg ccgttgaag atatttttt ctttacgaa 
      gcgaagaca aatctatag atacagtca gtgtttctt 
      acttgaaac cacctgctc gctaccctg actctgttc 
      aggaccagg aattcgaag tagacaatg agtctgttt 
      attcctttc ttatgaaac gagaatccg aagaaactc 
      taacaacac cggcacaaa gtcccgaaa aatcatcgg 
      aatctatcc ccaaagacg agaaacaaa tcgacagga 
      acatgtact acgaactgc acaggttat acactgcga 
      actacaaca cccagaagt ggaagaaca attaccttc 
      agaagaagt tagcacagt actataacc gccgaaaga 
      tgtatcatg tgtttgtcg tggacaagt cccaacagg 
      agtacgcca agatgtatg aaaaacttc tagaatcgt 
      ttgcagtca aacaggacg aagttgcaa gagaacaag 
      cagcgttgg agaacgaaa taagcgttc tgcgcaaca 
      aacatcgcg ccaaaagtg aagaagttg ccaacctcc 
      tggtggaac tccagcaag ccgaagcag acgtgagca 
      agcagttca gctacacgg ttctaaatg ctgcgaaaa 
      tggtcgaac gattcctca aacgccagc ggatgcagc 
      tgacacgtt tcaaggaaa tgcatcttg cttacataa 
      aattgcgaa acaagaaca acgaagcgc aagatactt 
      tggattcgc ttgataact tgggcccca actgccgcc 
      taatcgact acgaacagc tgaaaacgg ataacagga 
      acctttacg aaaagctgg aggagaagg aattgtact 
      taaactcgt taagacagg agtgtcgaa acgtgatgc 
      agatcttgg ctcatacga gggaaaaga cgaatgttg 
      tgaatttgg acattatga agtatcagg agcagctcg 
      atttggtgt tagcagatg gcatggaag cacgggaac 
      agttgagta tgatgaagc atgagaggg attacatca 
      ggcggcaga taaataaac taaagcttg agactggtt 
      tgcttggac gaccaaaac tgttaaggg atttggaaa 
      ggtcgatgg aagaagtgg aggaagggt tggatcaac 
      tagaggagt ataaagaag cttgtgagg tggcatctg 
      aaaccatgg tagctgtga ggaaatata ttagcagat 
      tgagcgaga caggggagt caggaaaga ttacgaaaa 
      gaattcagt tctgagcaa gtgggtttt agagaatgt 
      aatgtaggt gttaataaa ttcgctagc tcttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY