Status
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Gene information
General information
TC032488
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003701,TC003700
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 12922125..12930002
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032488
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
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    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03602

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032488 RA
      MKTSYFFTC WKKTETLQL MHMMTKQDI KFNFVKNEK 
      NAIIQALDE KSMTRYGYI LDTRCQNFK NLLSEKIST 
      VNMRLSHPW LILSAQLNT STSWEVVLK NVNLRMDSN 
      VKFAIPEDE STFNIFDVY NPAHQLGES PVITEFEIW 
      NGSSLQMQQ TKTFYLQRR DMSHIKLRS GSVVLISNH 
      SGTFEEYML DSRFKQLDT MSKFHYPMF LLLEDVHNF 
      THSLKIEDK WFGNYQNGT DSGVGKLLY ENEIDITCT 
      GASQRPPRV DVYDYLMPS YHFRPCYIF RNPGTYNPL 
      ENQFLKPFT DTVCKPPEA FHSLDELGH SKLEIGIED 
      LPYTITWFE IMNDSDVQY IYKNKVFPP NAKKLNIYE 
      PDEGIAKVK QGGFAYHTQ LDTGYPIIA RTFDQEQIC 
      DITEIPMIP QVGAGIMLQ KKSQYKELF HITLRKIKE 
      VGSCLIIFF FKLYYSFYN MSMSEGGVS IDTVATNEV 
      NLTENESPA SPETEALQE EAPIDVVGE QKLEETQEN 
      EAEKDAPVG EINRANVTE ENEKKGAEE EETPVMPVE 
      KESVEEGDV VKEEPVQVD QKKDAQEEE TPVIPIEEG 
      NVAKEESMQ VDQEEKSSP VQVDEKEKT TEAVDKNIE 
      DVKSVAGTE EYDFGHLVA PDAISEMSF ALLPEELPK 
      IEESSKETI ADQSETEEP KKKKTSKKK KIRPSIVKR 
      ESDEYPSLR ISGSQSLLI TGDFAIAPI GSKPQLPSL 
      YSEATEDEG EVEEEEEVS EEEEIHEMT EAERLEYYQ 
      KYDRLSKEH DEVKLKNDE LLRRLANYY KRKKMYHVL 
      HEGKVQLDA QKKYEKQLD DYSLQAEQS KKEQLALQT 
      EIADLKQRC TVQERETNE AFEKLQREE FEVGKEIIN 
      SEAGKGHEE KFIERYLKR QRIQLNNLV KVRYDYIKI 
      RNKLNETQE ALNAIDNLG SNLHLIDYE QLKLDNRNL 
      QDKFEERDL ELNRVRRKC QNAVQILAH RREKNAALD 
      ADITYLNER LEGVSGEYE DVREQLNQL KMERDGYRA 
      ATNKIIANS GLLSRPTLL RDLMNTMEE MEELEKRLL 
      DIKWECETV NNKVKKIRE NMASETKMN KIRYIMEGK 
      EFSDSFSGA TLSNVMVGT VAG
    CDS sequence
    • >TC032488 RA
      atgaaaacg tcttatttc tttacttgt tggaagaag 
      accgaaaca ttgcagtta atgcacatg atgaccaaa 
      caagatatt aagtttaat tttgtgaaa aacgaaaaa 
      aacgcaata attcaggcg ctggatgaa aaaagtatg 
      acccgttat ggatacatt ttagataca agatgccaa 
      aattttaaa aatttgtta tcagaaaaa atctctaca 
      gttaatatg agattatcg catccctgg ctaatactt 
      tcagcacaa ttgaatact tccacctct tgggaagtt 
      gtcttaaaa aatgtgaat ttaagaatg gattcaaat 
      gtaaaattt gcaattcct gaagacgaa tccactttt 
      aatatcttt gatgtttac aatcccgcc caccaactc 
      ggtgaatca cctgtgata acagaattt gagatttgg 
      aacggctca agcctgcaa atgcagcaa acaaaaaca 
      ttttattta caacggaga gacatgtct catataaaa 
      ctacgatct ggaagtgta gttttgatc agcaatcat 
      tctggaacc tttgaagag tatatgtta gactccaga 
      tttaaacaa ctcgacacc atgagcaag tttcattac 
      cctatgttt cttttgttg gaggatgtc cataatttc 
      acacactct ttaaaaatt gaagacaaa tggtttggt 
      aattaccaa aatggtaca gactcaggt gttggaaaa 
      ttattatac gaaaatgaa attgatata acatgtaca 
      ggggcatcg caaagaccc ccaagagtg gacgtttat 
      gattattta atgccctct tatcatttc agaccttgt 
      tacattttc agaaaccca gggacttac aacccgctc 
      gaaaaccaa tttttgaaa ccatttact gacacggtt 
      tgcaaacct ccggaagct ttccacagt ttggatgag 
      ctgggacat agcaaactg gaaatagga atcgaagat 
      ttgccttat accatcacc tggttcgag attatgaac 
      gacagtgat gtccagtac atttataaa aataaagtg 
      tttccacca aacgcaaaa aaactgaac atttatgag 
      ccagatgaa ggaatcgcc aaagttaaa caaggagga 
      tttgcgtat cacactcag ctagacaca ggatatccc 
      attattgcc aggaccttc gaccaggag caaatttgc 
      gatataact gaaattcca atgatacct caagtcgga 
      gctggaatt atgctgcag aagaaatcg caatataaa 
      gaactgttc cacataact ctgcgaaaa attaaagaa 
      gtcggttcg tgtttaata atttttttc tttaagtta 
      tattattcg ttttacaat atgtctatg tctgaagga 
      ggagtgagt atagataca gtggccaca aacgaggtg 
      aatttaact gaaaatgag tctcctgca agtcctgag 
      acagaagct ttgcaagag gaagcccca attgacgtt 
      gtcggggaa caaaagtta gaggaaacg caggaaaat 
      gaagcagaa aaggatgca ccggttggt gaaattaat 
      agagccaat gtcacagaa gaaaatgag aaaaagggt 
      gcagaagaa gaagaaaca ccagtaatg ccagtagaa 
      aaagaaagt gtggaagaa ggtgatgta gtaaaagaa 
      gaaccggtc caagttgat cagaaaaag gatgcacaa 
      gaagaagaa acgccagtg ataccaata gaagaaggt 
      aatgtagca aaagaagaa tcgatgcaa gttgatcag 
      gaagaaaaa tcatctccg gtccaagtt gatgagaaa 
      gagaaaaca actgaagct gtagataaa aatattgaa 
      gatgtaaaa agcgtagct ggaacagaa gaatatgac 
      tttgggcat ttagtggca ccggatgca atatcagaa 
      atgtcattt gccttacta ccagaagaa ctgcccaaa 
      atcgaagaa tcgtctaaa gagacaata gcagatcag 
      tcggaaaca gaagaacca aaaaagaag aaaacatca 
      aaaaagaaa aaaatacga cctagcatt gtaaaaaga 
      gagtcagac gaatatccg agtcttcgc atatcaggc 
      agtcaatcg cttcttatc actggagat tttgcaatt 
      gccccaatt ggcagcaaa ccccaactg ccatcactt 
      tactccgaa gccacagaa gacgaagga gaagttgaa 
      gaagaggaa gaagtgtca gaagaagaa gaaatacac 
      gaaatgact gaagcagaa cgcttggaa tattaccaa 
      aagtacgac cgcctttcc aaagaacac gacgaagtg 
      aagctcaaa aacgatgag cttctgagg agacttgca 
      aactactac aagaggaaa aaaatgtac catgtgtta 
      cacgaaggc aaggtccag ttagacgct cagaaaaaa 
      tacgaaaag cagttggac gattacagc ctccaggct 
      gaacaaagc aagaaagaa cagttagcc cttcagacg 
      gagattgca gatttgaag caacgatgc acagttcag 
      gaacgagaa actaatgaa gctttcgaa aaactgcaa 
      cgtgaggag tttgaggtc gggaaagaa ataatcaat 
      tcggaagcg ggcaaaggc catgaggag aaatttatc 
      gagaggtat ttgaagcga caaagaatt caactaaat 
      aatttggtc aaagtgcgc tatgactat atcaaaatc 
      cgaaacaag ttgaacgag acgcaggaa gccctgaac 
      gctattgat aatctcggc tcaaatctc catctcatc 
      gactatgag caacttaaa ctagataac cggaatctt 
      caagataag ttcgaagag agagacttg gaattgaac 
      agagtgcgg cgaaagtgc cagaacgcc gtccagatt 
      ttggcccat cgcagggaa aagaacgca gctttagat 
      gcggatata acttacttg aatgaaaga ctcgaaggt 
      gtaagtggc gagtatgag gacgtcagg gaacagctc 
      aatcagtta aagatggaa agggacggt tacagggct 
      gccactaat aagataata gcgaatagt gggttgttg 
      agcagacca acgttgctg agggacttg atgaacact 
      atggaggaa atggaagaa ttggaaaaa aggttgctt 
      gatatcaaa tgggaatgt gagactgtg aacaacaaa 
      gtaaagaag attagggaa aatatggct tcggaaacg 
      aaaatgaac aaaatcagg tatataatg gaagggaaa 
      gaattttca gatagcttt tctggggcg actctcagt 
      aatgtaatg gttggaact gtggccggt tag
    mRNA sequence
    • >TC032488 RA
      aagtagaaa taatatgaa aacgtctta tttctttac 
      ttgttggaa gaagaccga aacattgca gttaatgca 
      catgatgac caaacaaga tattaagtt taattttgt 
      gaaaaacga aaaaaacgc aataattca ggcgctgga 
      tgaaaaaag tatgacccg ttatggata cattttaga 
      tacaagatg ccaaaattt taaaaattt gttatcaga 
      aaaaatctc tacagttaa tatgagatt atcgcatcc 
      ctggctaat actttcagc acaattgaa tacttccac 
      ctcttggga agttgtctt aaaaaatgt gaatttaag 
      aatggattc aaatgtaaa atttgcaat tcctgaaga 
      cgaatccac ttttaatat ctttgatgt ttacaatcc 
      cgcccacca actcggtga atcacctgt gataacaga 
      atttgagat ttggaacgg ctcaagcct gcaaatgca 
      gcaaacaaa aacatttta tttacaacg gagagacat 
      gtctcatat aaaactacg atctggaag tgtagtttt 
      gatcagcaa tcattctgg aacctttga agagtatat 
      gttagactc cagatttaa acaactcga caccatgag 
      caagtttca ttaccctat gtttctttt gttggagga 
      tgtccataa tttcacaca ctctttaaa aattgaaga 
      caaatggtt tggtaatta ccaaaatgg tacagactc 
      aggtgttgg aaaattatt atacgaaaa tgaaattga 
      tataacatg tacaggggc atcgcaaag acccccaag 
      agtggacgt ttatgatta tttaatgcc ctcttatca 
      tttcagacc ttgttacat tttcagaaa cccagggac 
      ttacaaccc gctcgaaaa ccaattttt gaaaccatt 
      tactgacac ggtttgcaa acctccgga agctttcca 
      cagtttgga tgagctggg acatagcaa actggaaat 
      aggaatcga agatttgcc ttataccat cacctggtt 
      cgagattat gaacgacag tgatgtcca gtacattta 
      taaaaataa agtgtttcc accaaacgc aaaaaaact 
      gaacattta tgagccaga tgaaggaat cgccaaagt 
      taaacaagg aggatttgc gtatcacac tcagctaga 
      cacaggata tcccattat tgccaggac cttcgacca 
      ggagcaaat ttgcgatat aactgaaat tccaatgat 
      acctcaagt cggagctgg aattatgct gcagaagaa 
      atcgcaata taaagaact gttccacat aactctgcg 
      aaaaattaa agaagtcgg ttcgtgttt aataatttt 
      tttctttaa gttatatta ttcgtttta caatatgtc 
      tatgtctga aggaggagt gagtataga tacagtggc 
      cacaaacga ggtgaattt aactgaaaa tgagtctcc 
      tgcaagtcc tgagacaga agctttgca agaggaagc 
      cccaattga cgttgtcgg ggaacaaaa gttagagga 
      aacgcagga aaatgaagc agaaaagga tgcaccggt 
      tggtgaaat taatagagc caatgtcac agaagaaaa 
      tgagaaaaa gggtgcaga agaagaaga aacaccagt 
      aatgccagt agaaaaaga aagtgtgga agaaggtga 
      tgtagtaaa agaagaacc ggtccaagt tgatcagaa 
      aaaggatgc acaagaaga agaaacgcc agtgatacc 
      aatagaaga aggtaatgt agcaaaaga agaatcgat 
      gcaagttga tcaggaaga aaaatcatc tccggtcca 
      agttgatga gaaagagaa aacaactga agctgtaga 
      taaaaatat tgaagatgt aaaaagcgt agctggaac 
      agaagaata tgactttgg gcatttagt ggcaccgga 
      tgcaatatc agaaatgtc atttgcctt actaccaga 
      agaactgcc caaaatcga agaatcgtc taaagagac 
      aatagcaga tcagtcgga aacagaaga accaaaaaa 
      gaagaaaac atcaaaaaa gaaaaaaat acgacctag 
      cattgtaaa aagagagtc agacgaata tccgagtct 
      tcgcatatc aggcagtca atcgcttct tatcactgg 
      agattttgc aattgcccc aattggcag caaacccca 
      actgccatc actttactc cgaagccac agaagacga 
      aggagaagt tgaagaaga ggaagaagt gtcagaaga 
      agaagaaat acacgaaat gactgaagc agaacgctt 
      ggaatatta ccaaaagta cgaccgcct ttccaaaga 
      acacgacga agtgaagct caaaaacga tgagcttct 
      gaggagact tgcaaacta ctacaagag gaaaaaaat 
      gtaccatgt gttacacga aggcaaggt ccagttaga 
      cgctcagaa aaaatacga aaagcagtt ggacgatta 
      cagcctcca ggctgaaca aagcaagaa agaacagtt 
      agcccttca gacggagat tgcagattt gaagcaacg 
      atgcacagt tcaggaacg agaaactaa tgaagcttt 
      cgaaaaact gcaacgtga ggagtttga ggtcgggaa 
      agaaataat caattcgga agcgggcaa aggccatga 
      ggagaaatt tatcgagag gtatttgaa gcgacaaag 
      aattcaact aaataattt ggtcaaagt gcgctatga 
      ctatatcaa aatccgaaa caagttgaa cgagacgca 
      ggaagccct gaacgctat tgataatct cggctcaaa 
      tctccatct catcgacta tgagcaact taaactaga 
      taaccggaa tcttcaaga taagttcga agagagaga 
      cttggaatt gaacagagt gcggcgaaa gtgccagaa 
      cgccgtcca gattttggc ccatcgcag ggaaaagaa 
      cgcagcttt agatgcgga tataactta cttgaatga 
      aagactcga aggtgtaag tggcgagta tgaggacgt 
      cagggaaca gctcaatca gttaaagat ggaaaggga 
      cggttacag ggctgccac taataagat aatagcgaa 
      tagtgggtt gttgagcag accaacgtt gctgaggga 
      cttgatgaa cactatgga ggaaatgga agaattgga 
      aaaaaggtt gcttgatat caaatggga atgtgagac 
      tgtgaacaa caaagtaaa gaagattag ggaaaatat 
      ggcttcgga aacgaaaat gaacaaaat caggtatat 
      aatggaagg gaaagaatt ttcagatag cttttctgg 
      ggcgactct cagtaatgt aatggttgg aactgtggc 
      cggttagga tcttgtagt gtttgtagt gttgataag 
      taattgtac ggttagtga caggtgtat tttttgtta 
      gggacttat tgtagggac gtgtatatt tacagcaat 
      aaaatctat atgtattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY