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Gene information
General information
TC003694 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid17: 1257603..1263414
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003694
    iBeetle screen iB_03601
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003694 RA
      MTHAKEKVR KHECAVCQK KFLTKHDLK KHLRVHTDE 
      RPYVCKECN QGFRQAGSL KNHVAAKHS LGLPTLQTY 
      VCNYCNKEF PVKERLRLH IRIHTGVKP YQCRMCPKT 
      FARGGQLVQ HMRTHTGVK PYTCDICSS AFTCSANLK 
      LHIKGHLEI RDYVCDICG KKFYRRDAL KKHLTCYHG 
      NLKAFHCKI CNKQLKGHL PQHMRVHKK DKPHGCAHC 
      GARFAQRSQ LTVHQRIHS GEKPYRCQV CWKAFAHST 
      ALKLHIRRH TGEKPFVCV LCENVSFSQ LPHLKKHML 
      CIHKSSKPY VCSHCRSFH QTKNDLDGH YAKCQEYYN 
      NLHQKREID VNAIDPPMS LEKMRFLLA VLLKKISSQ 
      ERLEELGFN KRLIDDILV DSITSSGRE PCKDTSLSV 
      AEKLKKNVE ILLEWTVPK QYMEKFKNE QRSTEELLE 
      ELTS
    CDS sequence
    • >TC003694 RA
      atgactcac gccaaagag aaagtgcga aagcacgaa 
      tgcgcagtc tgccaaaaa aaattcctc accaaacac 
      gatctcaaa aagcacctt cgagtgcac accgacgaa 
      cgaccttac gtgtgcaaa gagtgcaat cagggcttc 
      cgtcaggcc ggctctctt aaaaatcac gtagccgcc 
      aagcactcc ctcggacta ccaaccttg caaacgtac 
      gtttgtaac tattgcaac aaggagttt ccggttaag 
      gagcgtctg aggctccac attcgcatc cacacgggg 
      gtgaagccc taccagtgc cgcatgtgc cccaaaacc 
      ttcgccaga ggggggcag ttggtccag cacatgcgc 
      acccacact ggggtcaag ccctacacg tgcgatatt 
      tgctcgtcg gcgttcacc tgttcagcc aacttgaag 
      ttgcacatc aagggccat ctggagatt agagactac 
      gtgtgcgac atctgtggg aagaagttc tacagaagg 
      gacgcacta aagaaacac ctgacgtgc tatcacggc 
      aacttgaag gccttccat tgcaaaatc tgtaataaa 
      cagttgaaa gggcactta cctcagcac atgagagtg 
      cataagaag gacaagccg catgggtgt gcccattgt 
      ggggcgagg ttcgcgcaa aggtcgcag ttgaccgtg 
      catcagagg atccactcg ggggagaag ccctaccgg 
      tgccaagtc tgctggaag gcctttgcc cactcgact 
      gcactcaag ctccacatc cgacggcac acgggggag 
      aagcccttc gtgtgtgtg ctgtgtgag aacgtttcg 
      ttctcgcag ttgccgcac ctgaagaag cacatgctt 
      tgcatacat aagagttcg aaaccgtac gtttgtagc 
      cactgccgg agcttccac cagaccaag aacgatctc 
      gacggtcac tatgccaag tgtcaggag tattacaat 
      aatttgcac caaaaacgc gagattgac gttaacgcc 
      attgacccg cctatgtct ttggagaag atgaggttt 
      cttttagcc gtactatta aaaaagatc tcgtcgcag 
      gaacgactg gaagagctg gggtttaat aaaagactc 
      atcgatgat atccttgtt gactcgata acgagttcg 
      gggcgggaa ccgtgcaaa gacacgagt ctttcagtc 
      gctgagaag cttaaaaag aacgtggag attctgctc 
      gagtggacg gtccccaaa cagtacatg gagaagttt 
      aaaaacgaa caaagatca accgaggag cttctagaa 
      gaattgact tcgtaa
    mRNA sequence
    • >TC003694 RA
      agtttttga cattaaaaa caccatccc cacaatcac 
      aatggtaaa ctaaaacta aaattcact ttaattcac 
      cacatttct atcaagatg cacggttta atggtgagg 
      tgatggttc ggcgtgtcc agagatggc gcccgccga 
      ctctcttta gatcactct aacatttct ttggtggtt 
      gggacataa aaagctcgc acccggtgc cggtttgca 
      tgttaacat tctagtcac aaaaagcca taaaatgac 
      tcacgccaa agagaaagt gcgaaagca cgaatgcgc 
      agtctgcca aaaaaaatt cctcaccaa acacgatct 
      caaaaagca ccttcgagt gcacaccga cgaacgacc 
      ttacgtgtg caaagagtg caatcaggg cttccgtca 
      ggccggctc tcttaaaaa tcacgtagc cgccaagca 
      ctccctcgg actaccaac cttgcaaac gtacgtttg 
      taactattg caacaagga gtttccggt taaggagcg 
      tctgaggct ccacattcg catccacac gggggtgaa 
      gccctacca gtgccgcat gtgccccaa aaccttcgc 
      cagaggggg gcagttggt ccagcacat gcgcaccca 
      cactggggt caagcccta cacgtgcga tatttgctc 
      gtcggcgtt cacctgttc agccaactt gaagttgca 
      catcaaggg ccatctgga gattagaga ctacgtgtg 
      cgacatctg tgggaagaa gttctacag aagggacgc 
      actaaagaa acacctgac gtgctatca cggcaactt 
      gaaggcctt ccattgcaa aatctgtaa taaacagtt 
      gaaagggca cttacctca gcacatgag agtgcataa 
      gaaggacaa gccgcatgg gtgtgccca ttgtggggc 
      gaggttcgc gcaaaggtc gcagttgac cgtgcatca 
      gaggatcca ctcggggga gaagcccta ccggtgcca 
      agtctgctg gaaggcctt tgcccactc gactgcact 
      caagctcca catccgacg gcacacggg ggagaagcc 
      cttcgtgtg tgtgctgtg tgagaacgt ttcgttctc 
      gcagttgcc gcacctgaa gaagcacat gctttgcat 
      acataagag ttcgaaacc gtacgtttg tagccactg 
      ccggagctt ccaccagac caagaacga tctcgacgg 
      tcactatgc caagtgtca ggagtatta caataattt 
      gcaccaaaa acgcgagat tgacgttaa cgccattga 
      cccgcctat gtctttgga gaagatgag gtttctttt 
      agccgtact attaaaaaa gatctcgtc gcaggaacg 
      actggaaga gctggggtt taataaaag actcatcga 
      tgatatcct tgttgactc gataacgag ttcggggcg 
      ggaaccgtg caaagacac gagtctttc agtcgctga 
      gaagcttaa aaagaacgt ggagattct gctcgagtg 
      gacggtccc caaacagta catggagaa gtttaaaaa 
      cgaacaaag atcaaccga ggagcttct agaagaatt 
      gacttcgta atcaaagcg ttagtacaa tattgtaga 
      cttgtaggt agcttactg actgttgca agtacccga 
      aattttgtt ttttctcat gactgttgc gctttttgg 
      tacaccgtt cttgcccaa gtgtgaaaa gtacaaaat 
      tcagcttta agaagcact cacatacca aagggagaa 
      gccaaaagg ttgtacagc atattacat ttagcttta 
      tttgttttt tatttaaga ccaaagaat attttttgt 
      tgtaatttt agttgttta agttttgtt ttggtcggc 
      gacctaccg actgcacca atttcgtgt catagcttc 
      taagtgcaa tacttgtgg caattttga gtttggcat 
      cacaacggt ataaactgt ttaaactaa ttttgtggt 
      gtgatgttt aaaaatata tcgacgtgg tggtgattt 
      tagttagga gtttttaat gttccttca agcatttgc 
      cagtcgagt gccatatta tatttacta atcattaaa 
      aagaaacaa aattttatt acaccacca cttaactaa 
      gtgttaatt atattttct aaacgtatg atttttaat 
      gtaacaaat actgttatg ggccggttt tcaaacctc 
      ttccactct gaaaataaa cgataaaat caact