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Gene information
General information
TC003692 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 12775205..12777342
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003692
    iBeetle screen iB_03600

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    urogomphi not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003692 RA
      MTNNEPKAP NIHDFCIIK PISRGAFGK VFLGCKKTN 
      MDVMYAIKV MKKTEMVNK NMVSQVVNE RNALALTKS 
      PFCVQLFYT LQTASSIYL VMDYMVGGD LKSLLSVYG 
      FFDEPMATF YVAEVCLAL QYLHSHSII HRDIKPDNM 
      LLSKEGHVK LTDFGLSCV QIHRDLEIS DFENATPNL 
      CTRTPGQLL SLTSHLSFG SGNSRNCDD SGANLERRF 
      DHNSSALSG VTFLSADGA SLQVTENSS YHTCASTNP 
      EDSTTSPVS RRGCYKLRG GDRKRKQRS PSPENPRKS 
      YIRTGLTGE MEILRVDST NSPKGVTFS TPVSNPKPN 
      KVIIKTTRF EIPKDSDCK TDENIVSPI TPKTPRTPY 
      RTPKSVKRG QWSSDQRIL GTPDYLAPE LLLQKGHNC 
      AVDWWALGV CFYEFVTGI PPFNDETPQ QVFKNILDH 
      NIEWPTDDE ALSKEVVDA IETLLTPDP EKRPQATEV 
      MKLEVFKNT DWKNLLQAQ PPFVPDPYD LTDTGYFQA 
      RNELLNFNI SNFDL
    CDS sequence
    • >TC003692 RA
      atgaccaac aacgagccg aaagctcca aatatccac 
      gatttttgc attattaag ccgataagt cgcggcgct 
      ttcggtaaa gtgtttttg gggtgcaaa aagacaaac 
      atggacgtg atgtacgcg attaaagtg atgaaaaag 
      accgaaatg gtaaataaa aatatggtg tctcaagtg 
      gtgaacgaa cgcaacgcc ctagccctc accaagagc 
      cccttctgc gtgcaattg ttctacacc cttcagacg 
      gcatcttcg atttacctc gtcatggac tacatggtc 
      gggggcgac ctcaagagt ctcctaagc gtgtacggg 
      ttttttgac gagcccatg gcaactttc tacgttgct 
      gaggtttgc ctcgcactt caatatctc cacagccat 
      agcataatc caccgagac attaaaccc gacaatatg 
      ctcctctcg aaagagggc cacgttaaa ttaaccgac 
      tttggcctc tcatgcgtg caaatccac cgagattta 
      gaaatttcc gatttcgaa aatgccact cctaatctc 
      tgtacacgc acccctggc caacttcta agcctcact 
      tctcatctc agtttcggt tcggggaac tcccggaac 
      tgcgacgac tctggggcc aatttagag cgacgtttc 
      gaccacaac agctcggca ttatcagga gttacgttt 
      ttatcagcc gatggggct tcactccaa gtcaccgaa 
      aacagttcc taccacacg tgcgcaagc acaaacccc 
      gaagactcc accacatct cccgtgagt agacgcggg 
      tgctacaag ctgcgaggg ggcgaccgg aagcggaaa 
      caacgctca ccgagtccg gaaaacccc cgaaaatcg 
      tacataaga acaggttta actggcgaa atggagatt 
      ttgagggtt gactcgaca aattccccc aagggggtc 
      actttttca acgccggtt tcgaaccca aaacccaac 
      aaagtgata atcaaaacg acgagattt gaaattccc 
      aaagactcg gattgcaaa actgatgaa aatattgtt 
      agtcctata acaccgaaa actccacga actccgtat 
      aggactccc aagtcggtt aagagaggg cagtggagc 
      agcgatcaa cgcattttg ggaactccc gattattta 
      gcccccgag ttattatta caaaagggg cacaactgt 
      gccgtggat tggtgggct ttgggagtt tgtttctac 
      gagtttgtg acagggatt cccccgttt aacgacgaa 
      acgcctcaa caagtattt aaaaatatc ctcgaccac 
      aacatcgaa tggcccact gatgatgaa gccctaagc 
      aaagaagtg gtcgacgct attgaaact ttactaact 
      ccggatccc gaaaagcga ccacaagcc accgaagtt 
      atgaaattg gaagttttc aaaaacacg gattggaaa 
      aatttactg caagctcaa ccgccgttt gtacctgat 
      ccttatgac ctgactgat accggatat tttcaagcc 
      aggaacgag ttacttaat tttaacata tcaaatttt 
      gatttgtga
    mRNA sequence
    • >TC003692 RA
      ccggccatg ttggcgacg cagcttccg atgggtgca 
      catcgtgta aaacatgta aaattcgcg aaattcctt 
      cctcaaaaa atgctattt tgaggcatt tactatgtg 
      cgagttgcc gtggtacgc attggccga aatctactt 
      gcagaaccg cgacaaatc caaagaaat ctactgttt 
      atgtagcag ggtttttgt aaggtaggt tatcgttct 
      gtcatctgt catttatta gttcgccat tcagattta 
      aatgcggca aaacggttt tctaaccaa tttttcaac 
      aaaacgatg ttataatga ccaacaacg agccgaaag 
      ctccaaata tccacgatt tttgcatta ttaagccga 
      taagtcgcg gcgctttcg gtaaagtgt ttttggggt 
      gcaaaaaga caaacatgg acgtgatgt acgcgatta 
      aagtgatga aaaagaccg aaatggtaa ataaaaata 
      tggtgtctc aagtggtga acgaacgca acgccctag 
      ccctcacca agagcccct tctgcgtgc aattgttct 
      acacccttc agacggcat cttcgattt acctcgtca 
      tggactaca tggtcgggg gcgacctca agagtctcc 
      taagcgtgt acgggtttt ttgacgagc ccatggcaa 
      ctttctacg ttgctgagg tttgcctcg cacttcaat 
      atctccaca gccatagca taatccacc gagacatta 
      aacccgaca atatgctcc tctcgaaag agggccacg 
      ttaaattaa ccgactttg gcctctcat gcgtgcaaa 
      tccaccgag atttagaaa tttccgatt tcgaaaatg 
      ccactccta atctctgta cacgcaccc ctggccaac 
      ttctaagcc tcacttctc atctcagtt tcggttcgg 
      ggaactccc ggaactgcg acgactctg gggccaatt 
      tagagcgac gtttcgacc acaacagct cggcattat 
      caggagtta cgtttttat cagccgatg gggcttcac 
      tccaagtca ccgaaaaca gttcctacc acacgtgcg 
      caagcacaa accccgaag actccacca catctcccg 
      tgagtagac gcgggtgct acaagctgc gagggggcg 
      accggaagc ggaaacaac gctcaccga gtccggaaa 
      acccccgaa aatcgtaca taagaacag gtttaactg 
      gcgaaatgg agattttga gggttgact cgacaaatt 
      cccccaagg gggtcactt tttcaacgc cggtttcga 
      acccaaaac ccaacaaag tgataatca aaacgacga 
      gatttgaaa ttcccaaag actcggatt gcaaaactg 
      atgaaaata ttgttagtc ctataacac cgaaaactc 
      cacgaactc cgtatagga ctcccaagt cggttaaga 
      gagggcagt ggagcagcg atcaacgca ttttgggaa 
      ctcccgatt atttagccc ccgagttat tattacaaa 
      aggggcaca actgtgccg tggattggt gggctttgg 
      gagtttgtt tctacgagt ttgtgacag ggattcccc 
      cgtttaacg acgaaacgc ctcaacaag tatttaaaa 
      atatcctcg accacaaca tcgaatggc ccactgatg 
      atgaagccc taagcaaag aagtggtcg acgctattg 
      aaactttac taactccgg atcccgaaa agcgaccac 
      aagccaccg aagttatga aattggaag ttttcaaaa 
      acacggatt ggaaaaatt tactgcaag ctcaaccgc 
      cgtttgtac ctgatcctt atgacctga ctgataccg 
      gatattttc aagccagga acgagttac ttaatttta 
      acatatcaa attttgatt tgtgaagtt ttatattgg 
      atattaata ataataaat gttttgttt ttgaaatgc 
      ctgttttta tttatgtga atttccaga cttaaggtt 
      ttcacatta ttgtaaatc tttgcagaa acaaagatg 
      acaatctca aacggaccg ttttctcat ccgaattca 
      gcgcttccg aagaggagc ttttaatcg aacaggaga 
      tcccgagtg agtgcattt cactgcctc agatattaa 
      aatataaag tcaaacaag aggtaagtg ataaataaa 
      aataatcta atacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY