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Gene information
General information
TC003668 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 12087356..12157333
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003668
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03596

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    head partiallyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen partially constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003668 RA
      MNNNGTTMS DYERIRLVI LGGAGVGKS CIIKRFLMN 
      TYSDKYRST IEDLYNREY DLGHMTLKV DILDTAGDM 
      QFPAMRRLC IATAHAFLL VYAVTSAPS FDCIKQCFE 
      EIREQRGDY QEIPIVVAG NKLDLTSTH REVRIEDVS 
      EWVYCELPK LRVKVLECS AKDDINIKE IFRSFLTLS 
      RIFPRDTED SSGLKRRSS AYVSASKGA RRAASPAPE 
      KDKQGGSSE ARETAKPRS RSLIRRASR KTKQQIRDA 
      HNGVEECTV S
    CDS sequence
    • >TC003668 RA
      atgaacaac aacggcacg accatgtcc gactacgag 
      cgcatccgc ctggtgatc ctcggcgga gccggcgtc 
      ggcaaaagc tgcatcatc aagcgcttc ctgatgaac 
      acctactcg gacaagtac cgcagtacc atcgaggac 
      ctctacaac cgcgagtac gacctcggc cacatgacg 
      ctcaaagtg gatatcctg gacacggcc ggagatatg 
      caattcccg gcgatgagg aggctgtgt atcgccaca 
      gctcacgcg tttctcctg gtgtatgct gtgacgtcg 
      gcgccgtcg tttgactgc atcaagcag tgtttcgag 
      gagattagg gaacagagg ggagactac caggaaata 
      cccatcgtg gtggccggg aataagctg gacttgacg 
      tccacgcac agggaggtc aggatcgag gatgtgtcc 
      gaatgggtc tactgcgag ttgcccaag ctgagagtg 
      aaagtgcta gagtgttcg gcaaaggac gacatcaac 
      atcaaggaa atattccgt tcgtttctg acgctctcg 
      aggatattt cctcgagat accgaagat tccagcggc 
      ttgaaacgc cgatccagc gcgtacgtt tcagcgagc 
      aaaggggcc agaagggcg gccagtcca gcccccgaa 
      aaggacaaa cagggcggc tcatcggag gccagagag 
      acggccaaa cccaggtcg aggtccctc atacggagg 
      gccagcagg aagactaaa cagcagatc agggacgcg 
      cataacggc gtcgaggag tgcaccgtc tcgtaa
    mRNA sequence
    • >TC003668 RA
      gtacttttg cagcgctgt gatattggc tcacggagc 
      ttgaagtgc attttgcga ctcgacgct tgtgagttg 
      cttttgcca gtgattgcc tcaaaacca tcaaaggga 
      ccagatgcc tctcctgta gaccatcca ccacatttc 
      caccctcca acatgctga aatgaagga ataagcgcc 
      acccctcgg ctccacccc caatgaaca acaacggca 
      cgaccatgt ccgactacg agcgcatcc gcctggtga 
      tcctcggcg gagccggcg tcggcaaaa gctgcatca 
      tcaagcgct tcctgatga acacctact cggacaagt 
      accgcagta ccatcgagg acctctaca accgcgagt 
      acgacctcg gccacatga cgctcaaag tggatatcc 
      tggacacgg ccggagata tgcaattcc cggcgatga 
      ggaggctgt gtatcgcca cagctcacg cgtttctcc 
      tggtgtatg ctgtgacgt cggcgccgt cgtttgact 
      gcatcaagc agtgtttcg aggagatta gggaacaga 
      ggggagact accaggaaa tacccatcg tggtggccg 
      ggaataagc tggacttga cgtccacgc acagggagg 
      tcaggatcg aggatgtgt ccgaatggg tctactgcg 
      agttgccca agctgagag tgaaagtgc tagagtgtt 
      cggcaaagg acgacatca acatcaagg aaatattcc 
      gttcgtttc tgacgctct cgaggatat ttcctcgag 
      ataccgaag attccagcg gcttgaaac gccgatcca 
      gcgcgtacg tttcagcga gcaaagggg ccagaaggg 
      cggccagtc cagcccccg aaaaggaca aacagggcg 
      gctcatcgg aggccagag agacggcca aacccaggt 
      cgaggtccc tcatacgga gggccagca ggaagacta 
      aacagcaga tcagggacg cgcataacg gcgtcgagg 
      agtgcaccg tctcgtaaa gacttttgt ggagaaagc 
      tcgagatgg tttgcaacc gcacaatgt aacgaattt 
      tactctaag tgtatcctg tatcaaggt ttagcatta 
      caagccgtc ccaactgaa aaatcctcc tcagtgtag 
      tgtgtcttg ttcaaagtg tttatttac atcgggtct 
      acatgcatg actgtttca gttggcaat actgaagtg 
      gcgaaactg ccaaatatc tcaccagtg ttgcttgct 
      aactcaaac aatcgttcg caactctcg tactcccga 
      tatatcaac aaaaaatag tcggagata cagcgtgtg 
      ccagcatta ttgaccttc aatatcaca aaatcaata 
      accaaggtt cacaatttt ttggacaca ctttgtaaa 
      tgtgcgtaa tcatactca tacacggtg actttttaa 
      aacgagcca aataaaatg taagtttga aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY