Status
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Gene information
General information
TC031040
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003632
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 9785690..9839154
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031040
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03594

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    cuticle remnants - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva posterior not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not completely molted - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031040 RA
      MAAYAQFGY SYPSASQVL PLLVSGQPT TNSGSSPAS 
      GGGSLSPGG SAGVPGTLS PGGTSSTGP TQCCESGRP 
      LVTDPVTGQ SVCSCQYDP ARLAALQYP RSVGVYGAP 
      YPSTDQNPY PSIGVESSA FYSPLSNPY ALKDAGGTA 
      ADMSAWTSA GLQPTTGYY PYDPALAAY GYGAGYDLA 
      ARRKNATRE STATLKAWL NEHKKNPYP TKGEKIMLA 
      IITKMTLTQ VSTWFANAR RRLKKENKM TWEPKNKTD 
      DDDDALVSD SDDKEKDEE DKRDDTDMH GRIKSERNG 
      KDLDDDDMN DDDRKDDLL GNNMHHILG NTFGLKPEM 
      KTSDCGVPL PPSKPKIWS LADTAACKT PPPPPPQQQ 
      QWLGSNGFA LPSSRYAGS GFLPSHCQQ GFPEVQTDT 
      PPQTPPSMK LPSVAGNLP CIGTAAATA ANSGYSQSG 
      FLGGFGRLQ SPHRPPPPQ AQMTAPTTD SAAFKPFYK 
      RTVGTKRLR HSSLPTTEC GTHRATYKE EKKHFFLPK 
      HERRVRVAG VSRSQLDPI DPSARWMVA GGPPSPEPM 
      GKVQQIQIV WVWSPC
    • >TC031040 RB
      MAAYAQFGY SYPSASQLL VSGQPTTNS GSSPASGGG 
      SLSPGGSAG VPGTLSPGG TSSTGPTQC CESGRPLVT 
      DPVTGQSVC SCQYDPARL AALQYPRSV GVYGAPYPS 
      TDQNPYPSI GVESSAFYS PLSNPYALK DAGGTAADM 
      SAWTSAGLQ PTTGYYPYD PALAAYGYG AGYDLAARR 
      KNATRESTA TLKAWLNEH KKNPYPTKG EKIMLAIIT 
      KMTLTQVST WFANARRRL KKENKMTWE PKNKTDDDD 
      DALVSDSDD KEKDEEDKR DDTDMHGRI KSERNGKDL 
      DDDDMNDDD RKDDLLGNN MHHILGNTF GLKPEMKTS 
      DCGVPLPPS KPKIWSLAD TAACKTPPP PPPQQQQWL 
      GSNGFALPS SRYAGSGFL PSHCQQGFP EVQTDTPPQ 
      TPPSMKLPS VAGNLPCIG TAAATAANS GYSQSGFLG 
      GFGRLQSPH RPPPPQAQM TAPTTDSAA FKPFYKRTV 
      GTKRLRHSS LPTTECGTH RATYKEEKK HFFLPKHER 
      RVRVAGVSR SQLDPIDPS ARWMVAGGP PSPEPMGKV 
      QQIQIVWVW SPC
    CDS sequence
    • >TC031040 RA
      atggcagcc tatgcgcag tttgggtac tcgtatccg 
      tcggcttct caggtactt cctctgttg gtgtcgggg 
      cagccgacg acgaactcg gggtcgtcg cccgcgtcc 
      gggggcggc tcgctgagc cccggcggg tcggcgggc 
      gtccccggg acgctgtca cctgggggc acgtcctcg 
      acgggcccg acgcagtgc tgcgagtcg gggcgaccc 
      ctcgtcacg gaccccgtg acgggccaa agcgtctgc 
      tcttgtcag tacgatcca gcgcgactt gcggccctg 
      cagtacccc cgaagtgtc ggtgtctac ggcgccccc 
      tacccctcc acggaccaa aacccctat cccagtata 
      ggcgtcgaa agttcggcc ttttactct cccctgagc 
      aatccctac gctttgaag gatgctggc gggacagca 
      gcagacatg tctgcgtgg accagcgcc ggcctccag 
      cccacgacg ggttattac ccctacgat ccggccctg 
      gcagcctac gggtacggt gccgggtac gatctggcg 
      gcgaggagg aagaacgcc acgagagag tcgacggcc 
      accctcaaa gcctggctc aacgagcac aagaaaaat 
      ccatatccg accaaaggc gagaagatc atgcttgcg 
      attatcacc aagatgacg ctgacgcaa gtctcgacg 
      tggtttgcg aacgcgcga cgaaggctc aaaaaggag 
      aacaaaatg acgtgggag cccaagaat aaaacggac 
      gatgacgac gacgcttta gtctccgac agcgacgac 
      aaggagaaa gatgaagag gacaaacgg gacgacaca 
      gacatgcac ggccggata aaatcggaa cgaaacggc 
      aaagattta gacgacgac gacatgaac gacgacgac 
      cgaaaggac gatttgttg ggcaacaac atgcaccac 
      atcctcggc aacaccttc ggcctcaag ccggagatg 
      aagacgtcg gactgcggc gtgccgctg ccgccctcc 
      aagccgaag atttggtcg ctggcggac accgccgcc 
      tgcaagacg ccgccgccc ccgccgccg cagcagcag 
      cagtggctg gggagcaac ggcttcgcc ttgccgagc 
      tcgaggtac gcggggagc ggcttcttg cctagtcac 
      tgtcagcag gggtttccc gaagtgcag acggacacg 
      ccgccccag acgccgccc agcatgaag ctgcccagt 
      gtggcgggg aatctgccc tgcatcggc acggcggcg 
      gccacggcg gcgaatagc ggatactcg cagtcgggg 
      ttcttgggc ggcttcggc cggctgcag tcgccccat 
      cggccgccg ccgccgcaa gcacagatg accgccccc 
      acgacggac agtgctgcc ttcaaaccc ttctacaaa 
      aggacagtg ggcactaaa aggctgcga cattcaagt 
      ttgcccaca accgaatgc gggacgcac cgagccaca 
      tataaggaa gaaaaaaaa catttcttt ctcccaaag 
      catgaacgg agggttcgt gtcgccggt gtgagccgc 
      tcccagctg gaccccatc gacccatcc gctagatgg 
      atggtcgcc ggagggccc ccgtcacca gaaccaatg 
      ggcaaagtg caacaaata caaatagtg tgggtgtgg 
      agcccgtgt taa
    • >TC031040 RB
      atggcagcc tatgcgcag tttgggtac tcgtatccg 
      tcggcttct cagctgttg gtgtcgggg cagccgacg 
      acgaactcg gggtcgtcg cccgcgtcc gggggcggc 
      tcgctgagc cccggcggg tcggcgggc gtccccggg 
      acgctgtca cctgggggc acgtcctcg acgggcccg 
      acgcagtgc tgcgagtcg gggcgaccc ctcgtcacg 
      gaccccgtg acgggccaa agcgtctgc tcttgtcag 
      tacgatcca gcgcgactt gcggccctg cagtacccc 
      cgaagtgtc ggtgtctac ggcgccccc tacccctcc 
      acggaccaa aacccctat cccagtata ggcgtcgaa 
      agttcggcc ttttactct cccctgagc aatccctac 
      gctttgaag gatgctggc gggacagca gcagacatg 
      tctgcgtgg accagcgcc ggcctccag cccacgacg 
      ggttattac ccctacgat ccggccctg gcagcctac 
      gggtacggt gccgggtac gatctggcg gcgaggagg 
      aagaacgcc acgagagag tcgacggcc accctcaaa 
      gcctggctc aacgagcac aagaaaaat ccatatccg 
      accaaaggc gagaagatc atgcttgcg attatcacc 
      aagatgacg ctgacgcaa gtctcgacg tggtttgcg 
      aacgcgcga cgaaggctc aaaaaggag aacaaaatg 
      acgtgggag cccaagaat aaaacggac gatgacgac 
      gacgcttta gtctccgac agcgacgac aaggagaaa 
      gatgaagag gacaaacgg gacgacaca gacatgcac 
      ggccggata aaatcggaa cgaaacggc aaagattta 
      gacgacgac gacatgaac gacgacgac cgaaaggac 
      gatttgttg ggcaacaac atgcaccac atcctcggc 
      aacaccttc ggcctcaag ccggagatg aagacgtcg 
      gactgcggc gtgccgctg ccgccctcc aagccgaag 
      atttggtcg ctggcggac accgccgcc tgcaagacg 
      ccgccgccc ccgccgccg cagcagcag cagtggctg 
      gggagcaac ggcttcgcc ttgccgagc tcgaggtac 
      gcggggagc ggcttcttg cctagtcac tgtcagcag 
      gggtttccc gaagtgcag acggacacg ccgccccag 
      acgccgccc agcatgaag ctgcccagt gtggcgggg 
      aatctgccc tgcatcggc acggcggcg gccacggcg 
      gcgaatagc ggatactcg cagtcgggg ttcttgggc 
      ggcttcggc cggctgcag tcgccccat cggccgccg 
      ccgccgcaa gcacagatg accgccccc acgacggac 
      agtgctgcc ttcaaaccc ttctacaaa aggacagtg 
      ggcactaaa aggctgcga cattcaagt ttgcccaca 
      accgaatgc gggacgcac cgagccaca tataaggaa 
      gaaaaaaaa catttcttt ctcccaaag catgaacgg 
      agggttcgt gtcgccggt gtgagccgc tcccagctg 
      gaccccatc gacccatcc gctagatgg atggtcgcc 
      ggagggccc ccgtcacca gaaccaatg ggcaaagtg 
      caacaaata caaatagtg tgggtgtgg agcccgtgt 
      taa
    mRNA sequence
    • >TC031040 RA
      tcgctatca gatcgcagt ggcgtatag atcgtatgg 
      tgtttgttt tgcatgtga cacgatgga ttttgaggc 
      gagaatata gacatgtca tgaagcttt gtgttgtgg 
      gaccactcg gatttttag tgtgtgggt gactgtgat 
      ttgttcggg gcgagtttt tagactgag tgcaatggc 
      agcctatgc gcagtttgg gtactcgta tccgtcggc 
      ttctcaggt acttcctct gttggtgtc ggggcagcc 
      gacgacgaa ctcggggtc gtcgcccgc gtccggggg 
      cggctcgct gagccccgg cgggtcggc gggcgtccc 
      cgggacgct gtcacctgg gggcacgtc ctcgacggg 
      cccgacgca gtgctgcga gtcggggcg acccctcgt 
      cacggaccc cgtgacggg ccaaagcgt ctgctcttg 
      tcagtacga tccagcgcg acttgcggc cctgcagta 
      cccccgaag tgtcggtgt ctacggcgc cccctaccc 
      ctccacgga ccaaaaccc ctatcccag tataggcgt 
      cgaaagttc ggcctttta ctctcccct gagcaatcc 
      ctacgcttt gaaggatgc tggcgggac agcagcaga 
      catgtctgc gtggaccag cgccggcct ccagcccac 
      gacgggtta ttaccccta cgatccggc cctggcagc 
      ctacgggta cggtgccgg gtacgatct ggcggcgag 
      gaggaagaa cgccacgag agagtcgac ggccaccct 
      caaagcctg gctcaacga gcacaagaa aaatccata 
      tccgaccaa aggcgagaa gatcatgct tgcgattat 
      caccaagat gacgctgac gcaagtctc gacgtggtt 
      tgcgaacgc gcgacgaag gctcaaaaa ggagaacaa 
      aatgacgtg ggagcccaa gaataaaac ggacgatga 
      cgacgacgc tttagtctc cgacagcga cgacaagga 
      gaaagatga agaggacaa acgggacga cacagacat 
      gcacggccg gataaaatc ggaacgaaa cggcaaaga 
      tttagacga cgacgacat gaacgacga cgaccgaaa 
      ggacgattt gttgggcaa caacatgca ccacatcct 
      cggcaacac cttcggcct caagccgga gatgaagac 
      gtcggactg cggcgtgcc gctgccgcc ctccaagcc 
      gaagatttg gtcgctggc ggacaccgc cgcctgcaa 
      gacgccgcc gcccccgcc gccgcagca gcagcagtg 
      gctggggag caacggctt cgccttgcc gagctcgag 
      gtacgcggg gagcggctt cttgcctag tcactgtca 
      gcaggggtt tcccgaagt gcagacgga cacgccgcc 
      ccagacgcc gcccagcat gaagctgcc cagtgtggc 
      ggggaatct gccctgcat cggcacggc ggcggccac 
      ggcggcgaa tagcggata ctcgcagtc ggggttctt 
      gggcggctt cggccggct gcagtcgcc ccatcggcc 
      gccgccgcc gcaagcaca gatgaccgc ccccacgac 
      ggacagtgc tgccttcaa acccttcta caaaaggac 
      agtgggcac taaaaggct gcgacattc aagtttgcc 
      cacaaccga atgcgggac gcaccgagc cacatataa 
      ggaagaaaa aaaacattt ctttctccc aaagcatga 
      acggagggt tcgtgtcgc cggtgtgag ccgctccca 
      gctggaccc catcgaccc atccgctag atggatggt 
      cgccggagg gcccccgtc accagaacc aatgggcaa 
      agtgcaaca aatacaaat agtgtgggt gtggagccc 
      gtgttaagc gattgcaat ctgttgtat atttgtgta 
      aaagcattt atttatatc tgtgataaa gaacaaaat 
      gtctaccaa gtggcgttg acgtttttc ggcaatggg 
      acttgtagt ttcttgaaa atagaagcg agcacttac 
      gtttgtaac ttgttgtgt atcatcaca agtcaagct 
      agttgtatt tcaggattc acctatttt attgttacg 
      ttcttttaa ataattaga aaattgttg tgtatatta 
      aaagtacca tatcatgga tttattatt aaactaaca 
      tttattttg ttggctcct agccgcaaa ttgtacatt 
      attttatct gtttgtaaa tagatcagt gtgtataga 
      aggatatta taattataa ttgtcttga ttaaaatat 
      taacagaga c
    • >TC031040 RB
      tcgctatca gatcgcagt ggcgtatag atcgtatgg 
      tgtttgttt tgcatgtga cacgatgga ttttgaggc 
      gagaatata gacatgtca tgaagcttt gtgttgtgg 
      gaccactcg gatttttag tgtgtgggt gactgtgat 
      ttgttcggg gcgagtttt tagactgag tgcaatggc 
      agcctatgc gcagtttgg gtactcgta tccgtcggc 
      ttctcagct gttggtgtc ggggcagcc gacgacgaa 
      ctcggggtc gtcgcccgc gtccggggg cggctcgct 
      gagccccgg cgggtcggc gggcgtccc cgggacgct 
      gtcacctgg gggcacgtc ctcgacggg cccgacgca 
      gtgctgcga gtcggggcg acccctcgt cacggaccc 
      cgtgacggg ccaaagcgt ctgctcttg tcagtacga 
      tccagcgcg acttgcggc cctgcagta cccccgaag 
      tgtcggtgt ctacggcgc cccctaccc ctccacgga 
      ccaaaaccc ctatcccag tataggcgt cgaaagttc 
      ggcctttta ctctcccct gagcaatcc ctacgcttt 
      gaaggatgc tggcgggac agcagcaga catgtctgc 
      gtggaccag cgccggcct ccagcccac gacgggtta 
      ttaccccta cgatccggc cctggcagc ctacgggta 
      cggtgccgg gtacgatct ggcggcgag gaggaagaa 
      cgccacgag agagtcgac ggccaccct caaagcctg 
      gctcaacga gcacaagaa aaatccata tccgaccaa 
      aggcgagaa gatcatgct tgcgattat caccaagat 
      gacgctgac gcaagtctc gacgtggtt tgcgaacgc 
      gcgacgaag gctcaaaaa ggagaacaa aatgacgtg 
      ggagcccaa gaataaaac ggacgatga cgacgacgc 
      tttagtctc cgacagcga cgacaagga gaaagatga 
      agaggacaa acgggacga cacagacat gcacggccg 
      gataaaatc ggaacgaaa cggcaaaga tttagacga 
      cgacgacat gaacgacga cgaccgaaa ggacgattt 
      gttgggcaa caacatgca ccacatcct cggcaacac 
      cttcggcct caagccgga gatgaagac gtcggactg 
      cggcgtgcc gctgccgcc ctccaagcc gaagatttg 
      gtcgctggc ggacaccgc cgcctgcaa gacgccgcc 
      gcccccgcc gccgcagca gcagcagtg gctggggag 
      caacggctt cgccttgcc gagctcgag gtacgcggg 
      gagcggctt cttgcctag tcactgtca gcaggggtt 
      tcccgaagt gcagacgga cacgccgcc ccagacgcc 
      gcccagcat gaagctgcc cagtgtggc ggggaatct 
      gccctgcat cggcacggc ggcggccac ggcggcgaa 
      tagcggata ctcgcagtc ggggttctt gggcggctt 
      cggccggct gcagtcgcc ccatcggcc gccgccgcc 
      gcaagcaca gatgaccgc ccccacgac ggacagtgc 
      tgccttcaa acccttcta caaaaggac agtgggcac 
      taaaaggct gcgacattc aagtttgcc cacaaccga 
      atgcgggac gcaccgagc cacatataa ggaagaaaa 
      aaaacattt ctttctccc aaagcatga acggagggt 
      tcgtgtcgc cggtgtgag ccgctccca gctggaccc 
      catcgaccc atccgctag atggatggt cgccggagg 
      gcccccgtc accagaacc aatgggcaa agtgcaaca 
      aatacaaat agtgtgggt gtggagccc gtgttaagc 
      gattgcaat ctgttgtat atttgtgta aaagcattt 
      atttatatc tgtgataaa gaacaaaat gtctaccaa 
      gtggcgttg acgtttttc ggcaatggg acttgtagt 
      ttcttgaaa atagaagcg agcacttac gtttgtaac 
      ttgttgtgt atcatcaca agtcaagct agttgtatt 
      tcaggattc acctatttt attgttacg ttcttttaa 
      ataattaga aaattgttg tgtatatta aaagtacca 
      tatcatgga tttattatt aaactaaca tttattttg 
      ttggctcct agccgcaaa ttgtacatt attttatct 
      gtttgtaaa tagatcagt gtgtataga aggatatta 
      taattataa ttgtcttga ttaaaatat taacagaga 
      c
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